chemical mediation of reproductive processes

... majority of meroplankton in the world’s oceans are comprised of the propagules of algae or the eggs and larvae of benthic invertebrates and fish Little is known of the chemical defenses of the propagules ... are aware of no laboratory studies of the chemical feeding-deterrent properties of meroplankton that have an adult planktonic phase, nor of meroplankton comprised of the propagules of marine ... recent study of chemical defenses of the lecithotrophic progeny of a marine invertebrate is that of Slattery et al.,70 who examined the developing embryos and larvae of the tropical soft coral Sinularia...

Ngày tải lên: 12/08/2014, 00:21

... mineralization [1] Page of Figure qRTPCR analysis of FGF-2 regulated genes associated with mineralization; qRTPCR analysis of gene reduction of genes over a 24 hours of treatment with FGF-2 shows ... result of a specific sequential regulation of gene expression from the early phase of osteoblast proliferation to the final steps of mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: ... expression of mineralizing genes Hughes-Fulford and Li Journal of Orthopaedic Surgery and Research 2011, 6:8 http://www.josr-online.com/content/6/1/8 Figure qRTPCR analysis of gene induction of proliferation...

Ngày tải lên: 20/06/2014, 04:20

... mineralization [1] Page of Figure qRTPCR analysis of FGF-2 regulated genes associated with mineralization; qRTPCR analysis of gene reduction of genes over a 24 hours of treatment with FGF-2 shows ... result of a specific sequential regulation of gene expression from the early phase of osteoblast proliferation to the final steps of mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: ... expression of mineralizing genes Hughes-Fulford and Li Journal of Orthopaedic Surgery and Research 2011, 6:8 http://www.josr-online.com/content/6/1/8 Figure qRTPCR analysis of gene induction of proliferation...

Ngày tải lên: 20/06/2014, 07:20

... purity of the cell preparation This method typically yields a cell suspension containing 70–100% of monocytes with a contamination range between 0–30% of lymphocytes Purity of 88%– 100% of monocytes ... the absence of IL-12 mediated induction of endogenous IFNγ secretion when compared with A549/PBMC co-cultures (See also Table 1) % inhibition of IP-10 secretion in co-cultures Effects of IFN-γ antibody ... with a presence of lymphocytes, most likely to provide a source of IFN-γ The interaction of monocytes and lung epithelial cells are made by direct cellcell contact (A) Addition of recombinant...

Ngày tải lên: 12/08/2014, 15:21

... globular domain of Rpb1 and will be the focus of future studies to determine biological significance of this post-translational modification Mark G Goebl, PhD Chair v TABLE OF CONTENTS LIST OF TABLES ... comparison of detected proteins from MS/MS data 78 viii LIST OF FIGURES PDB-Viewer/Pov Ray generated model of complete ribbon structure for RNAPII complex 2 Model of regulation of ... number of biochemical activities including: suppression of promoter-proximal stalling of RNAPII, promoter DNA melting, RNA Polymerase II loading onto the template strand, and stimulation of productive...

Ngày tải lên: 24/08/2014, 09:44

... experimental failures more bearable i TABLE OF CONTENTS Acknowledgements Table of Contents Summary List of Tables List of Figures List of illustrations Abbreviations List of publications Chapter Introduction ... (TEMED) 2.1.19 Stacking gel (5%) 0.83 ml of 30% acrylamide mix with 0.63 ml of M Tris-HCl, pH 6.8, 3.4 ml of water, 25 µl of 20% SDS and 50 µl of 10% APS with µl of TEMED 32 2.1.20 Protein loading ... in level of Bubr1 and Mad2 after knockdown of p73 105 Fig 3.28: Different mechanism of action between p73 and Bubr1 and Mad2 in regulation of mitotic cell death 107 Fig 3.29: Knockdown of Bubr1...

Ngày tải lên: 13/09/2015, 21:21

... the Decree of the Council of Ministers of the USSR, June 1990, ‘On Measures of Securing the Implementation of the Edict of the Presidium of the USSR Supreme Soviet of 26 November 1984 “On Intensifying ... accompaniment of the CCGS John A 40 41 42 43 See Arts and 10 of the NSR Regulations; Arts 14 and 15 of the Economic Zone Edict; Arts and 15 of the Environmental Edict; and Art 11 of the Decree of the ... (Article 7); control of navigation (Article 8); temporary suspension of navigation in particular areas of the NSR (Article 9); removal of vessels off the NSR (Article 10); limitation of liability for...

Ngày tải lên: 01/11/2013, 09:20

... KIND2-binding specificity of residues 702–744 of MAP2, we performed a pulldown assay of combinations of EGFP-fused KIND1 or KIND2 of v-KIND with GST-fused CD2-1-6-1 or CD2-1-6-2 of MAP2 We successfully ... series of GST-fused deletions of the CD2-1 region (Fig 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the ... full-length v-KIND; DKIND1, deletion of KIND1; DKIND2, deletion of KIND2; DKIND1 + 2, deletion of both KIND1 and KIND2; DRasN, deletion of RasN; DGEF, deletion of RasGEF; KIND1, KIND1 domain; KIND2,...

Ngày tải lên: 14/02/2014, 19:20

... genetic diseases [47] Most analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target gene expression, have ... treatment of the chick face with RA, and this is accompanied by an increase in apoptosis [73] The authors of this study ascribe the regulation of outgrowth of limb buds and patterning of the digits ... A.-K Bosserhoff growth retardation, AP-2s play vital roles in cell proliferation Besides the functions of AP-2s in physiological processes, they have also crucial roles in pathological processes...

Ngày tải lên: 16/02/2014, 09:20

... of actin with RNA polymerases and components of the transcription machinery in the nucleus The role of actin in transcription and its regulation Actin is both a major cytoskeletal component of ... transcription of all three RNA polymerases Specifically, b-actin has been identified as a component of RNA polymerase II (Pol II) pre-initiation complexes (PICs) Injection of anti-actin Ig into the nuclei of ... component of pre-mRNP particles, and is incorporated into pre-mRNAs by binding to a specific subset of RNA-binding proteins [4,22]; and (h) actin is a component of PICs and depletion of actin prevents...

Ngày tải lên: 07/03/2014, 01:20

... concentrations of chemical ligand for induction, and show high background activity of the reporter or transgene in the absence of ligand [1,2,12,30,31] We have previously demonstrated the utility of a ... of the transient expression studies, we selected a double mutant (V395I + Y415E) of CfEcR (CfEcRVY) for additional stable transformation experiments to evaluate regulation of the expression of ... Improvement of EcR gene switch Fig Schematic representation of gene switch constructs (A) The pK80VCH9 VP16 AD fusion of RXR CH9 was cloned into the pKYLX80 (pK80) vector (B–E) GAL4 DBD fusions of the...

Ngày tải lên: 16/03/2014, 06:20

... group of LeuB387 together with O atom of c-OH of SerB386; Ng2 is in H bonding with Od1 of AsnB241 In the free enzyme, a bridging water molecule W360 bonded with c-OH of SerB386 and Ng2 of ArgB263, ... structure [19] give evidence of polar environment around ArgB263 It consists of O atoms of the main chain CO groups of LeuB387 and TrpB240, Od1 atom of AsnB241 and Oc atom of SerB386 and is expected ... part determines the position of the substrate within the S1¢-binding subsite O atoms of the main chain CO groups of LeuB387 and TrpB240, Od1 atom of AsnB241, and Oc atom of SerB386 and is expected...

Ngày tải lên: 16/03/2014, 16:20

... translocation of AR to the nucleus Facilitation of nuclear transport of AR by HIP-1 depends on its C-terminal nuclear localization signal for HIP-1 In addition to that of AR, mediation of transcriptional ... may not be independent of each other Detailed descriptions of these processes are beyond the scope of this review In the present review, we specifically focus on the role of Htt-interacting protein ... dominant nature of the disease suggests a toxic gain -of- function of the mutated protein that disrupts normal cellular functions and causes neuronal death [3] Loss -of- function of the wild-type...

Ngày tải lên: 23/03/2014, 07:20

... protection of DNA from nucleases is a general property of NAPs, regardless of the type of nuclease tested (NAPs have been shown to prevent exonuclease III – another type of nuclease – degradation of ... levels of spermine in solution rendered the genomic DNA extremely sensitive to the action of DNase I We believe that this is a result of the resolubilization of DNA and the massive exposure of the ... form of NAP The concentration of the 8000 Da form of NAP in the nucleus did not vary throughout the phases of the cell cycle In this study, based on the investigation (by gel electrophoresis) of...

Ngày tải lên: 23/03/2014, 15:20

... 2003 Ó FEBS 2003 nucleotides upstream of the STOP codon of the ORF of interest and on the 3¢ end, 65–70 nucleotides of the genomic region downstream of the ORF of interest Transformants were recovered ... upstream of SFH1 Name SFH1/1D SFH1/1R Downstream of SFH1 SFH1/2D SFH1/2R Control primer 5¢ upstream of SFH1 SFH1/3D Control primer inside SFH1 SFH1/3R 5¢ upstream of SFH2 SFH2/1D SFH2/1R Downstream of ... the lipid composition of the respective deletion strains To correlate not only the function of Sec14 homologues to that of Sec14p, but also to compare the sites of action of these polypeptides,...

Ngày tải lên: 23/03/2014, 18:20

... energy status of the cell is an important determinant of GAL gene induction [15,25–27] This suggests that the availability of metabolic energy is a rate-limiting step in the synthesis of GAL enzymes ... 5% of the total soluble proteins when the cell grows on galactose as sole carbon source [28,29] Phosphorylation of S699 of Gal4p has been shown to be important for activating transcription of ... methods MRG19 as a regulator of iso-1-cytochrome C Disruption of MRG19 results in the de-repression of the CYC1 promoter We reported previously that multiple copies of MRG19 suppress CYC1 driven...

Ngày tải lên: 31/03/2014, 08:20

... number of degrees of freedom of the whole system is the sum of degrees of freedom of individual nodes corrected for variables common to neighbouring nodes This approach is useful in education of chemical ... accounting purposes monitoring of heat recovery systems monitoring of operation of chemical reactors regular balancing of separation systems balancing as a part of control of processes If the balancing ... balance of even very complicated processes The other area of mass and energy balancing concerns the analysis of operating processes The advent of process computers brought the huge amount of process...

Ngày tải lên: 01/04/2014, 10:15

... properties of denture base material related to the effect of level of residual monomer, and concluded that the percentage of higher levels of residual monomer effected on the tensile properties of denture ... from injection of the sample as shown in the diagram The average of 12 readings of each sample of heat-cure and self-cure denture base materials were shown in Table The results of MMA content ... comparison of 11 soft lining materials as a function of accelerated aging J Prosthetic Dentistry, 69, 114–119 Sanders, J.L, Levin, B & Reitz, P.V (1991) Comparison of the adaptation of acrylic...

Ngày tải lên: 07/08/2014, 14:20

... Labsystems, Uxbridge, UK) using the Delta soft II.4 software programme (DeltaSoft Inc, Hillsborough, NJ, USA) Results are expressed as the mean concentration of triplicate cultures ± standard deviation ... differential utilization of NFκB for the expression of CC and CXC chemokines in this system We also examined the role of NFκB in the Tck-cell contactdependent production of chemokines in monocytes ... regulation of macrophage chemokine production is a general property of activated T cells Several other groups have also shown the importance of T-cell contact in regulating the production of cytokines...

Ngày tải lên: 09/08/2014, 08:23

... is one of the rarest codons (8.7% of all serine codons and 0.45% of all 61 codons) The UCA codon is exclusively translated by the tRNA UGA [17] The genome of E coli has only one copy of this ... characteristics did not affect the fitness of the host cell We did find, however, that the extent of utilization of two particular codons (UCA and CAU) does correlate Page of 10 negatively with a cell’s ... investigate the effect of regions with less than optimal codons, for each gene we defined the ‘bottleneck’ as a region of a fixed number of codons, n, where the (harmonic) mean of the codons’ tAI...

Ngày tải lên: 09/08/2014, 22:23