checking the integrity of a genomic sequence between two markers

Báo cáo khoa học: Insights into the design of a hybrid system between Anabaena ferredoxin-NADP+ reductase and bovine adrenodoxin pot

Ngày tải lên : 31/03/2014, 07:20

... mixed in the cell measuring compartment and lM CYP1 1A1 was placed in the second compartment After the samples were made anaerobic, an excess of NADPH was added to the FNR/Adx mixture to allow Adx ... represents the initial rate of the reaction, vs the Adx concentration shows an almost linear correlation (Fig 3A) , suggesting that the formation of the optimal complex between the two proteins limits the ... Kinetic parameters for the anaerobic reaction between FNRrd and Adxox Data calculated from steady-state spectra recorded in Fig 2A at 414 nm Adx concentration dependence of (A) kobs1, kobs2 and V0...

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Báo cáo khoa học: " In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence" docx

Ngày tải lên : 12/08/2014, 18:22

... 5'CAGTTAAGCTTCGCAGAGCTCGTTTAGTGA-3' and 5'CAGTTGGATCCAGTGTCGACGACGGTGAC-3', primers that included splice sites The PCR product was digested A Kozak sequence adjacent to the ATG start codon greatly ... 5'-GGTGTGAATTCCATGAAGTGGGTAACCTTTAT-3' and 5'-GGTGTCTCGAGCGTAAGCCTAAGGCAGCTTGA-3' and cloned in frame with V5 epitope and polyhistidine tag The CMV intron A was amplified by PCR from VR1012 (Vical) ... when the gene was amplified from H1 The HSA gene, V5 and His6 tags were amplified using 5'-GGTATGCGGCCGCTTATGAAGTGGGTAACCTTTAT-3' without Kozak or using 5'-GTATGCGGCCGCCACCATGAAGTGGGTAACCTTTAT-3'...

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Báo cáo y học: " Modification of a loop sequence between -helices 6 and 7 of virus capsid (CA) protein in a human " pot

Ngày tải lên : 12/08/2014, 23:21

... Igarashi T, Martin MA, Khamsri B, Hatcho K, Yamashita T, Fujita M, Uchiyama T, Adachi A: Generation of HIV-1 derivatives that productively infect macaque monkey lymphoid cells Proc Natl Acad ... Structure of the chimeric HIV-1/SIVmac clones and a summary of their replication capabilities Structure of the chimeric HIV-1/SIVmac clones and a summary of their replication capabilities White bars ... Plishka RJ, Buckler-White A, Shibata R, Martin MA: Determination of a statistically valid neutralization titer in plasma that confers protection against simian-human immunodeficiency virus challenge...

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Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

Ngày tải lên : 07/03/2014, 21:20

... shown Black geometrical shapes are additional domains, as indicated ANOGA, Anopheles gambiae; ASHGOS, Ashbya gossypii; BRARE, Brachydanio rerio; CANDGLA, Candida glabrata; CIONA, Ciona intestinalis; ... translocation to the nucleus Although functional analyses of individual domains have not been addressed, the global context of the domain analyses allows us to draw a more general picture of the ... DIDO-related apoptosis occurs as a result of alterations in DNA regulation caused by chromatin instability Computational analyses Although all splice variants share common domains, long regions of the...

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Báo cáo khoa học: Functional importance of a conserved sequence motif in FhaC, a prototypic member of the TpsB/Omp85 superfamily ppt

Ngày tải lên : 23/03/2014, 03:20

... well between the two proteins, arguing against a major conformational change of the loop Structural data are available in the Protein Data Bank under the accession numbers 2QDZ (FhaCWT) and 3NJT ... was used as a template with the following primers : R450AUp (5¢-GACGAGTACACGGT GGCCGGATACAACCTCAGGA-3¢) and R450ALo (5¢-TC CTGAGGTTGTATCCGGCCACCGTGTACTCGTC-3¢); Y452AUp (5¢-ACACGGTGCGCGGAGCCAACCTCAAG ... by the rmsds for ˚ the Ca superimposition of the barrel (0.44 A) and the ˚ ) The overall rmsd is 0.53 A ˚ two POTRAs (0.61 A (Fig 4A) The relative orientation of the two POTRA domains is the same...

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Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins ppt

Ngày tải lên : 31/03/2014, 01:20

... purpurea) NP_053827, Athal2 (Arabidopsis thaliana IscA2) AC005825.3, Athal3 (Arabidopsis thaliana IscA3), AC006921.5, A_ vinIscA (Azotobacter vinelandii IscA) T44283, A_ vinOrf6 (Azotobacter vinelandii ... GATAATCTGAAAACC-3¢, MOslr1417-4 5¢-GGAT TTACCACAACCAgctGTTTGATTAGCATT-3¢ and MOslr1417-5 5¢-CCAAAGGATTTACCggcgCCACAGG TTTG-3¢ (lower case type indicates the introduced mutations, new cleavage sites ... variant C4 4A was compared it appeared that the cluster formation was retarded (data not shown) This demonstrates that the cluster formation at the variant C4 4A was impaired The variant C34/44/7 5A in...

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Báo cáo toán học: "A Combinatorial Proof of the Log-concavity of a famous sequence counting permutations" potx

Ngày tải lên : 07/08/2014, 08:22

... and decrease the entry of p that was one larger than p1 by one Let the obtained permutation be p Similarly, decrease q1 by 1, and increase the entry of q that was one larger than q1 by Let the ... = 1, then remove these entries, to get the permutations p∗ and q∗ (After natural relabeling, these are both permutations of length n − 1.) Because of the extreme values of at least one of the ... Rule was used In that case, to get the preimage of (t, u), we need to remove the first entry of t and the first entry of u, swap the permutations, and find the preimage of the resulting pair (¯,...

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Báo cáo toán học: "The degree of a q-holonomic sequence is a quadratic quasi-polynomial" docx

Ngày tải lên : 08/08/2014, 14:23

... equal to a quasi-polynomial if they agree for all but ﬁnitely many values Theorem 1.1 (a) The degree of a q-holonomic sequence is eventually a quadratic quasipolynomial (b) The leading term of a ... together with a set of multiplicities that satisfy a balancing condition Abstractly, a tropical curve is a balanced rational graph, and vice-versa; see [RGST05, Thm.3.6] There is a duality between ... independent of the spacial variables x of a diﬀerential operator The meaning of the variable q can be explained by Quantization, or from Tropical Geometry (where it is usually denoted by t) or from the...

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báo cáo khoa học: " The first three-dimensional visualization of a thrombus in transit trapped between the leads of a permanent dual-chamber pacemaker: a case report" docx

Ngày tải lên : 11/08/2014, 02:22

... reconstruction of the two- dimensional transesophageal data Three-dimensional offline reconstruction of the two- dimensional transesophageal data helped us to identify the origin of the thrombus 3D visualization ... interpreted patient data regarding the cardiac disease and was a major contributor in writing the manuscript PM performed the transthoracic echocardiography PM, TB and MP performed the transthoracal and ... the patient for publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this journal Maagh et al Journal of...

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Báo cáo y học: "The genome of Apis mellifera: dialog between linkage mapping and sequence assembly" pdf

Ngày tải lên : 14/08/2014, 18:20

... (1.43 and 3.37), orientation of a long scaffold (10.30) and remnants in a false position of the break of scaffold 6.37 This generally excellent co-linearity pleads in favor of the quality of the two ... mistakes remain within scaffolds, they should be below the level of resolution of the map (average 93 kb) This agreement could seem to be a circular argument as the map is the framework of the assembly ... Consortium, Waterston RH, Lindblad-Toh K, Birney E, Rogers J, Abril JF, Agarwal P, Agarwala R, Ainscough R, Alexandersson M, et al.: Initial sequencing and comparative analysis of the mouse genome Nature...

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The Duality of Memory and Communication in the Implementation of a Multiprocessor Operating System

Ngày tải lên : 12/09/2012, 15:05

... dirty pages • Data manager floods the cache This is rather similar in nature to a data manager which fails to free data, but is easier to detect and prevent • Data manager changes data A malicious ... service a data request for another thread in that task • Server tasks which cannot tolerate changing data, or which must ensure that all of the necessary data is available, may use a separate thread ... copied) data in a message • Data manager backs its own data Deadlock may occur if a data manager becomes blocked in a page fault waiting for data which it provides Fortunately, there are several techniques...

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List the components of a radio system

Ngày tải lên : 13/09/2012, 10:52

... – Advantages • Can carry up to three times the amount of data as TDMA • Transmissions are much harder to eavesdrop on • A would-be eavesdropper must also know the exact chip in which the transmission ... data to be sent – Imprints a unique address on the data – The longer the code is, the more users will be able to share the same channel – Number of chips in the code • Determines the amount of ... Divides the transmission time into several slots – Each user is assigned the entire frequency for the transmission • For a fraction of time on a fixed, rotating basis – Advantages • Uses the bandwidth...

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Cambridge.University.Press.Africans.The.History.of.a.Continent.Aug.2007.pdf

Ngày tải lên : 21/09/2012, 10:39

... languages belonged to a third, Nilo-Saharan family, which may have originated in the broad Saharan region at least as early as Afroasiatic Nilo-Saharan may be distantly related to the fourth family, ... taken Carthage a decade later, and extended their power across the region sub-saharan africa Whether Carthage transmitted metalworking to sub-Saharan Africa is one of the mysteries of African ... religions, and alphabetic literacy that sub-Saharan Africa partially shared with the Eurasian core Partial isolation meant that cultural phenomena took distinctively African forms Partial integration...

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Guest the meaning of a word

Ngày tải lên : 02/10/2012, 12:07

... (not, reversal) ex- (out of, former) pre- (before) re- (again, restore) un- (do the opposite of) ad- (to, toward) com-, con-, co- (with, together) en-, em- (in, into, to cover or contain) in- (into, ... (with, together) en-, em- (in, into, to cover or contain) in- (into, not) pro- (in favor of, before) sub- (under, beneath) ...

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Báo cáo y học: "The Impact of a Nationwide Antibiotic Restriction Program on Antibiotic Usage and Resistance against Nosocomial Pathogens in Turkey"

Ngày tải lên : 25/10/2012, 11:00

... non-parametric correlations A P value of less than 0.05 was regarded as significant Software package STATA 9.0 (USA) was used for the analysis Materials and Methods Results Hospital setting and antibiotic ... financial cost and resistance patterns of leading nosocomial pathogens (gram) and number of boxes were calculated from two databases, 1) Hospital pharmacy computer databases, and 2) International ... decreased and the trend of resistance rates became downwards after implementation of NARP The amount of money saved increased further After two years of NARP 5,389,155.82 USD saving occurred in the...

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The closeness of a foreign sales contract in Binh Minh Household Joint Stock Company

Ngày tải lên : 18/04/2013, 08:57

... engages mainly in the industry of household appliances, as well as the domains of real estate and logistics The group is also one of the largest manufacturing and exportation bases of electric appliances ... Generally, if possible, it is wise to adopt the internal law as governing law to the contract On the other hand, the parties can agree to adopt a certain world law regarding international trade ... days after of the buyer’s advice ARTICLE 8: ARBITRATION During the performance of the contract, if any dispute arises, the parties shall discuss to find solution in an amicable way If the parties...

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Experimental study on the performance of a prism-shaped integrated collector-storage solar water heater

Ngày tải lên : 05/09/2013, 15:28

... water ascends to the top of the tank It is originated from the fact that, after gaining heat from the solar radiation, the tank wall heats a thin vertical layer of water along the tank wall Part ... function of the density, specific heat capacity and the initial and massweighted average temperatures of water; with all the values, apart from the mass-weighted average temperature, are the same ... solar radiation and average temperature of the present experimental study and the theoretical results of [1] in January Figure 12 The time variation of solar radiation and stored energy of the...

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Factors influencing borrower’s behavior and decision making patterns in the success of a micro finance model

Ngày tải lên : 06/09/2013, 05:48

... populations The data was tabulated and analyzed through qualitative analysis of the gathered data, which reveal the behaviors and decision making patterns in lower income populations towards ... behavioral and attitudinal aspects of individuals An in-depth analysis in each of the broad parameters revealed the following: Educational Facet Education plays a vital role in shaping up a personality ... expectations The academia in Pakistan may facilitate the MFIs in producing relevant research work in understanding the broader social paradigms of borrowers, which may actually lead to the success...

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Tài liệu The message of a master - By John McDonald pdf

Ngày tải lên : 15/12/2013, 06:15

... the great masses of humanity are using the Law destructively, or partially so, and the scales are balanced against them Here and there, among the masses, we find an occasional outstanding figure ... inferior creatures as the beasts of the field, the birds of the air and the fish of the sea are bountifully supplied For any man, no matter what his station in life, to take the stand that it is the ... we know what we are talking about when we speak of the chaos of thoughts in the air http://www.RetrieveALover.com Page 33 The Message of a Master On the other hand, the man who has a set definite...

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