characterization of glycosaminoglycans by tandem vibrational microspectroscopy and multivariate data analysis

... extracts using GC-MS and multivariate data analysis Journal of Separation Science 2012, 35: 53-59 J Ching*, H.S Lin*, C.H Tan, H.L Koh Quantification of α- and βamyrin in rat plasma by gas chromatography-mass ... 118 4.4.2.1 Validation of GC-MS method for MVDA study 118 4.4.2.2 GC-MS analysis of all extracts and fractions 119 4.4.2.3 PCA and PLS-DA analysis of the extracts and fractions 122 4.4.2.4 ... known as Aryuveda) TCM and Aryuveda have been practiced for thousands of years, and their remedies usually comprise of mixtures of plant and/ or animal parts The combination of different components...

Ngày tải lên: 10/09/2015, 08:25

... matries similar to EBN, thereby resolving the issues of quality control and species of origin of EBN Classification of EBN according to its coloration, country of origin and production site could ... LIST OF TABLES LIST OF TABLES Table Examples of food authentication with the use of GC/MS and LC/MS 28 Table Examples of food authentication with the different types of multivariate ... acid data (C) milk and (D) infant formula from monosaccharide data 70 XII LIST OF FIGURES Figure 15 Column plots of (A) total EAA and (B) total amino acids of different types of samples...

Ngày tải lên: 09/09/2015, 11:13

... as a result of overpressure and upon the opening of the closed Letlok cell [7–9] The morphologies of the products were observed by SEM, HRSEM, TEM and HRTEM analysis The morphologies of the Nb2O5@C ... existence of 69.0 wt% of Nb and 31 wt% of O, which is very close to the theoretical value of Nb2O5 (Nb = 69.9 wt% and O = 30 wt%) The carbon peak originates from the carbon shell The peaks of copper ... excitonic or interband (valence conduction band) transitions of Nb2O5, which allows us to calculate the band gap Figure depicts the optical DRS of the Nb2O5 An estimate of the optical band gap is obtained...

Ngày tải lên: 22/06/2014, 22:20

... binding and identification of other IN-interacting cofactors involved in this process will contribute to a better understanding of the action of IN during HIV-1 replication and aid in development of ... than that of KR186,7AA The K186 and R187 of IN, by crystallographic studies, are known to lie in the dimer-dimer interface of IN [42,43] and F185 has been implicated for tetramerization of IN [44] ... T7-LEDGF/ p75 and YFP-IN in each sample was detected by WB with corresponding antibodies (Fig 2A, middle and lower panel) The host chromatin and LEDGF/p75 cofactor interaction data of all the IN...

Ngày tải lên: 12/08/2014, 04:20

... in 13C japonica and Z marina and Z marina LS: leaves and stems LS: leaves and stems RR: roots and rhizomes RR: roots and rhizomes is disturbed by breaking waves Typically, δ13C of plants decrease ... and rhizomes Meanwhile, Z marina in the leaves and stems has -9.7 ± 1.0‰, and in the roots and rhizomes has -9.6 ± 1.0‰ Fig shows that the leaves and stems as well as the roots and rhizomes of ... leaves and stems and about 36 kinds of fatty acids in the roots and rhizomes of Z japonica We focused on the fatty acid content of Z japonica , because we needed to exclude the influence of a...

Ngày tải lên: 05/09/2013, 10:17

... monstrosities and dermoid cysts, for example, we seem to catch forbidden sight of the secret workroom of Nature, and drag out into the light the evidences of her clumsiness, and proofs of her lapses of ... an unseen hand reaching down into the world of matter and the workings of bodily organization, and reining them to impersonal service and far-off ends All ethnologists and students of primitive ... encyclopedic collection of rare and extraordinary cases, and of the most striking instances of abnormality in all branches of medicine and surgery, derived from an exhaustive research of medical literature...

Ngày tải lên: 17/02/2014, 07:20

... edge and postspawning gonads Metamorphosis represents the ultimate stage of oyster development, and is characterized by the degeneration of larval tissues, such as the velum and the foot, and ... remodelling of larval tissues to produce adult tissues (i.e the development of the gills and the production of an adult shell), which is accompanied by significant growth of the soft body parts ... shell formation and body growth by the secretion of shell organic matrix and by cell proliferation As Cg-Clp2 appears to be expressed in both epithelial and conjunctive cell types of the mantle...

Ngày tải lên: 19/02/2014, 00:20

... 4, and were isolated by HPLC and used as substrates for AUH The AUH assay contained mM of the respective isomers of 3-MG-CoA in a total volume of 25 lL The reaction was started by addition of ... crotonyl-CoA, 3-hydroxybutyryl-CoA and 3-methylcrotonyl-CoA were used for the kinetic characterization of AUH The data are summarized in Table Overexpression of a mutant form of AUH and its activity on (E)-3-MG-1-CoA ... for h and the CoA products were HPLC-detected by their absorbance at 260 nm Peak produced small amounts of HMG-CoA and large amounts of free CoA Peak produced HMG-CoA and also large amounts of free...

Ngày tải lên: 19/02/2014, 07:20

... structure of munc18 according to the Ó FEBS 2002 Phosphorylation of syntaxin-1A by CKI and CKII (Eur J Biochem 269) 911 Fig Phosphorylation of syntaxin-1A (1–265, 1–190, 191–265) by CKI and CKII ... peptide from the HPLC (data not shown) To identify the site(s) of syntaxin-1A that are phosphorylated by CKIa, constructs 1–190 and 1–265 of syntaxin-1A were phosphorylated by CKIa and subjected to ... the residue(s) of syntaxin-1A phosphorylated by CKII Phosphorylated forms of syntaxin-1A (1–190 and 1–265) were digested with trypsin and the peptides were separated by HPLC (data not shown)...

Ngày tải lên: 22/02/2014, 04:20

... GluRS and GluTR, whose spectrum and DLS signal are shown in Fig S11 The migration positions of GluTR and GluRS, were determined by comparison with those of a homogeneous sample of GluRS (S) and of ... sheets, subjected to TLC and quantication of radiolabelled ADP and AMP The kinetics of [3H]AMP and [3H]ADP formation in the absence of tRNAGlu (black symbols) or of both L-Glu and tRNAGlu (grey symbols) ... ubiquity and essentiality of aaRS makes them of interest as targets of new anti-infectives [3] Their reaction formally consists of the activation of the amino acid by adenylation (Eqn 1) followed by...

Ngày tải lên: 07/03/2014, 03:20

... happened by the way; and we arrived on a morning of blue and gold We put up at a private hotel out of the way from fashionable thoroughfares; and, as my childhood and early youth were passed in England, ... THE CAR OF DESTINY BY C N AND A M WILLIAMSON Illustrations by Armand Both iv The Car of Destiny NEW YORK THE McCLURE COMPANY MCMVII OTHER BOOKS BY THE SAME AUTHORS Lady Betty ... nor looks were out of the way Yet what I said and did was said and done with no more guidance of the mind than the gestures and speech of a mechanical doll I was conscious only of the girl's eyes,...

Ngày tải lên: 07/03/2014, 11:20

... compilation ª 2006 FEBS M Burow et al Analysis of glucosinolate hydrolysis products by GC Dichloromethane extracts of ESP and NSP assays were analysed by GC-MS and by GC with flame ionization detection ... addition of Fe2+) to 87.2% (0.01 mm Fe2+) and 93.9% (0.5 mm Fe2+) of the total amount of hydrolysis products No epithionitrile was formed by myrosinase in the presence of Fe2+ without addition of ESP, ... of ESP measured in crude seed extracts of Lepidium sativum has been shown to increase in the presence of both Fe2+ and Fe3+ [24], whereas no effect of Fe2+ on the activity of ESP in Crambe abyssinica...

Ngày tải lên: 23/03/2014, 11:20

... GELGIYLVGDDSLFPWR and IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band ... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... substrate specificity and function of the Ndh complex We hope that further analysis of the Ndh subunits by N-terminal sequence analysis or mass spectrometry (MALDI-TOF-MS and MS ⁄ MS) will reveal...

Ngày tải lên: 23/03/2014, 13:20

... is replaced by isoleucine in HvTrxh2 (C) HvTrxh1 and HvTrxh2 are indicated Cereal sequences are in bold and indicated by lled circles Species are identied by initials and isoforms by italicised ... nmolặs)1ặnmol)1 and 13 lM Again, HvTrxh2 and TrxTa resembled each other with Vmax of 2.0 and 1.5 nmolặs)1ặnmol)1 and Km of 44 lM and 37 lM, respectively In the same assay, a Km value of  lM was ... appearance, and biochemical properties Further characterization of both proteins and identication of specic interaction partners will contribute to a fuller understanding of the functions of thioredoxin...

Ngày tải lên: 23/03/2014, 17:22

... oversaturated with ANS and fluorescence was measured with an excitation wavelength of 388 nm and a band pass of nm, and an emission wavelength of 473 nm with a band pass of nm Measurements were ... dehydrogenase, 150 kDa; apoferritin, 443 kDa; and thyroglobulin, 669 kDa are indicated by numbered arrows induced by expression of G and TS were similar to each other (P > 0.05) and significantly higher ... intensities, with the peak of G and TS at 348 nm and R at 360 nm The fluorescence intensity of R was the lowest and it was red-shifted in comparison to other samples The extent of tertiary structure...

Ngày tải lên: 30/03/2014, 20:20

... absence of specific cytokines and diffusible signaling proteins, VEGF by itself is not sufficient to trigger expression of numerous enzymes and proteins required for the development of a network of ... current analyses regardless of the intensity of the stain All protein profiles from the HIV-infected and uninfected cells were compared and analyzed by a variety of subtractive computer-based ... for the range of each protein data Full protein names and accession #s of each protein are provided in Table tant for the survival and maintenance of functional changes in the CD4 and CD8 cells...

Ngày tải lên: 18/06/2014, 15:20

... resolution [1,2] and condensation/packaging of the viral genome in IV particles [3] This is followed by encapsidation of a transcription complex, formation of a defined core, and reorganization of virion ... http://www.virologyj.com/content/4/1/78 of each A12L cleavage product will elucidate more details of regulation and function of VV proteolysis immunoblot analysis with A12L antisera and preimmune serum was used as a control (data ... β-mercaptoethanol, and 2% SDS), followed by treatment with Equilibration buffer (EB) I and II, which contained 200 mg of DTT and 250 mg of Iodoacetamide respectively in 10 mL of EB (6 M Urea, 2%...

Ngày tải lên: 18/06/2014, 18:20

... resolution [1,2] and condensation/packaging of the viral genome in IV particles [3] This is followed by encapsidation of a transcription complex, formation of a defined core, and reorganization of virion ... http://www.virologyj.com/content/4/1/78 of each A12L cleavage product will elucidate more details of regulation and function of VV proteolysis immunoblot analysis with A12L antisera and preimmune serum was used as a control (data ... β-mercaptoethanol, and 2% SDS), followed by treatment with Equilibration buffer (EB) I and II, which contained 200 mg of DTT and 250 mg of Iodoacetamide respectively in 10 mL of EB (6 M Urea, 2%...

Ngày tải lên: 20/06/2014, 01:20

... are of great interest and utility to a large cross-sections of agricultural and biomedical research community, selection of some isolates with natural improved characteristics and definitive characterization ... seuquence and its deduced polypeptide and results of these analyses are shown in Figs (5 and 6) In case of Polh-cr nucleotide seuquence, a phylogenetic tree was generated from sequence data of 38 ... analysis of data on the progression of mortality of S littoralis larvae, following exposure to the LC50s dosage using probit analysis modified for multiple observations over time [24] and Mathematica...

Ngày tải lên: 20/06/2014, 01:20

... positive: Most of us enjoy the extra comfort and the feeling of accomplishment that comes with both the process of saving and with the results—having more freedom of choice both now and in the future ... Jr and E B White’s classic book, The Elements of Style, you will recognize one of the original sources of inspiration for this book and why we are so brief If you are unfamiliar with Strunk and ... RULE OF 72 Do you know the amazing Rule of 72? If not, learn it now and remember it forever It’s easy, and it unlocks the mystery of compounding Here it is: X ϫ Y ϭ 72 That is, X (the number of...

Ngày tải lên: 21/06/2014, 08:20