... Each directory has a file ‘ ’ in it which is a reference to its parent directory After changing into the directory with chdir , i.e chdir paper you can return to the parent directory by doing chdir ... special meaning either to the shell or to UNIX These characters are called metacharacters If it is necessary to place these characters in arguments to commands without them having their special ... commands which are connected together, the standard output of each connected to the standard input of the next, is called a pipeline The pipe mechanism used to connect these commands is indicated...
Ngày tải lên: 15/12/2013, 20:15
... so-called "real world." Our thoughts cause our physical world to take form according to the electromagnetic or attractor fields we create According to the law of cause and effect, we attract ... or perceive it Atoms consist mainly of empty space! Since atoms continually exchange their subatomic particles with each other, there is a physical connection between all objects “We are all ... experiences, they easily relate to the concept that their negative thoughts cause their brain to secrete chemicals that immediately impair their performance This awareness helps them to be more objective...
Ngày tải lên: 27/01/2014, 02:20
Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspecific alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf
... Note the decrease in the aggregate on the stacking gel Next, we introduced the third mutation into TNSALP (1559delT -C5 06S ⁄ C5 2 1C ⁄ C5 77S) The aggregation state was dramatically changed in the cells ... decreased as the chase time elapsed However, this decline is not simply accounted for by the secretion of the mutant protein into the medium, as no band was detectable even in the h chase culture ... 1) This 66 kDa product becomes the 80 kDa form in the presence of the microsome Probably the increase in molecular mass is solely due to the acquisition of N-linked oligosaccharides, as supported...
Ngày tải lên: 19/02/2014, 17:20
Báo cáo khoa học: Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin potx
... of the MUC5AC sequence With use of the primer pair 5¢-TATTCTAGAG AAGAGGGCCT GGTGTGCCGG AACCAGGACC AGCAGGGACC CTTCAAG-3¢ (GH262) and 5¢-ACGCGCTAGC TCAATGATGA TGATGATGGT GCATGGGGGA CACTGGGACG CC-3¢ ... a cDNA clone corresponding to the 3¢-end of human MUC5AC inserted into a pBluescript vector, was used as a template for PCR With use of the primer pair 5¢-GCTTCTAGAC ACGAGAAGAC AACCC ACTCC C- 3¢ ... in the 3¢-end of the PCR product The PCR product was ligated into the XbaI site of the previously described pSM vector [26] This vector is based on the pEGFP -C1 vector (Clontech, Palo Alto, CA,...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... Ure2p, critical for assembly into fibrils To further identify the regions involved in Ure2p–Ssa1p interaction, we set up a chemical cross-linking strategy coupled to the identification of the chemically ... are denoted T and CT, respectively The protonated monoisotopic experimental masses (MH+exp) and the calculated mass difference (p.p.m.) with the theoretical monoisotopic mass of the identified peptide ... of the low abundance of cross-linked peptides and potential changes in their ionization properties [32] Because changes in the reactivity of amino-acid residues to the cross-linkers can be efficiently...
Ngày tải lên: 15/03/2014, 00:20
Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt
... HPLC-purified primer WT (5¢-AATTGATCCCGCCCG CCTCGTTTTCATCGATGAGACCTGGACGAAGACGA ACATGGCGCCGCTGCGGGGC-3¢) using 50 lCi of [c- 32P]ATP (3000 CiÆmmol)1; GE Healthcare) and 100 units of T4 polynucleotide ... a template for amplification of a 70 bp PCR product with [5¢-32P]-labeled S70ds ⁄ UP (5¢-AATTGATCCCGCCCGCCTC-3¢) and S70ds ⁄ DN (5¢-GCCCCG CAGCGGCGCCATGTT-3¢) primers For PCR, we added 2.5 · 10)3 ... product Other bands correspond to nonspeci c cleavage products The products expected from partial reverse splicing of the intron RNA into the insertion site in the DNA are shown at the top Solid...
Ngày tải lên: 15/03/2014, 09:20
Báo cáo Y học: Incorporation of 3-nitrotyrosine into the C-terminus of a-tubulin is reversible and not detrimental to dividing cells potx
... Tecnologica (BID 802/OC-AR), Consejo Nacional de Investigaciones Cientı´ ficas y ´ ´ Tecnicas (CONICET), Secretarı´ a de Ciencia y Tecnica de la Univer´ ´ sidad Nacional de Cordoba y Agencia Cordoba Ciencia ... Agencia Nacional de Promocion Cientı´ fica y Tecnologica de la Secretarı´ a de Ciencia y Tecnologı´ a del Ministerio de Cultura y ´ ´ ´ Educacion en el marco del Programa de Modernizacion Tecnologica ... care has reinforced efforts to understand mechanisms of cell injury and death during pathological conditions Idriss hypothesized [19,20] that the selective cytotoxic effect of TNFa (tumor necrosis...
Ngày tải lên: 17/03/2014, 10:20
C#1 introduction to programming and the c language potx
... Excellent Economics and Business programmes at: Please click the advert The perfect start of a successful, international career.” CLICK HERE to discover why both socially and academically the ... Introduction to C Introduction to C# A computer program is a family of commands executed in a speciic order that together solves a speciic task A program is written as a text document that contains ... it is because the compiler allocates space in the machine’s memory, and that when the name appears somewhere in the code, the translator must know the name’s meaning in order to check if the variable...
Ngày tải lên: 18/03/2014, 02:20
Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx
... primer (5¢-CCAAGCTTGTCGACGATGAGTGAGATATCC CGG-3¢) and a reverse primer (5¢-CGGGAATTCCTG CAGTTGCTTTCTCGCAGCAAC-3¢), and then cloned between the SalI and PstI sites of the DHFR fusion vector pJWL1030folA ... that is likely to further reduce the capacity of the fragment to bind metals The conformational change could move E271 away from the active site, hence stabilizing the structure of the domain In ... shown that ZnCl2 inhibits the activity of AMPP [1] Here, the presence of ZnCl2 in the dialysis buffer led to the precipitation of each of the three proteins Neither the intact protein nor the FEBS...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: The C-terminus of viral vascular endothelial growth factor-E partially blocks binding to VEGF receptor-1 docx
... the following primers: mV-for 5¢ (5¢-CATGGCGCGCCTGATGAAC TTTCTGCTGTCTTGG-3¢) and mV-NZ 2C 3¢ (5¢-CTGAC GCGTGCGGCGTCTTCTGGGCGGCCTTGTGGTCGT CGGTGGCGTGGTTGTGAACTTTGGTCTGCATTCA CATCGGCT-3¢) The PCR ... AGTTGCTCGTCGGCATAC-3¢, AscI site underlined) and NZ2-DC 3¢ (5¢-ACTCGAACGCGTTCGTGGTCTA CAATCGCA-3¢, MluI site underlined) A DNA fragment containing nucleotides 4–458 of VEGF-A and 55 nucleotides from the C- terminus ... described previously [15] A DNA fragment containing nucleotides 4–368 of ORFVNZ2VEGF was amplified by PCR from viral DNA with the following primers: NZ2-DC 5¢ (5¢-AGCGCCCGGCGCGCCAGA AGTTGCTCGTCGGCATAC-3¢,...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo Y học: The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains pptx
... (5¢-CGTTCTAGAGT GAGCAAGGGCGAGGAGCTG-3¢) and reverse primer B (5¢-ATCTACGTCGGCTAGCCTTGTACAGCTCGT CCATGCCGAG-3¢) The fragment was then ligated into the NheI site of the plasmid encoding His-tag-D4 The ... reaction primers used were 5¢-CTCAGGC TAGCAAGGGAAAAATAAACTTAGATC-3¢ (for D4 and DC-D4), 5¢-TCAGAGCTAGCAGTGGAGCCTATG TTGCACAG-3¢ (for DN-D4) and 5¢-TGGTGGTGG TGCTCGAGTGC-3¢ For the construction ... h at C in a SW55 rotor, 11 fractions of 0.4 mL each were collected from the top and the pellet was suspended in 0.4 mL of TNE buffer Fluorescence microscopy For fluorescence microscopic observation,...
Ngày tải lên: 23/03/2014, 21:20
Chapter 1 Introduction to the C Language
... (just-in-time) compiles this CIL into native code Program CIL native code • Only at this point can the OS execute the application Garbage Collection • This is the NET method of making sure that the ... semicolon (;) 11 Basic concepts in C# (cont.) • Namespace (p.51) • Namespaces are used as a means of categorizing items • Within a namespace, you can declare: • • • • • • another namespace class ... writing code • Properties Window toolbar – Alphabetic icon: arranges the properties alphabetically – Categorized icon: arranges the properties by category – Event icon: allows reactions to user actions...
Ngày tải lên: 13/05/2014, 11:30
The Strategy Gap Leveraging Technology to Execute Winning Strategies ppt
... hence, their strategic goals so they can act early to bring the tactical plan back on target Technology clearly has a role to play in facilitating this communication Failure to effectively communicate ... advance can be selected or corrections to the existing plan can be made to avoid or exploit the impact of any variances The ability to recognize and exploit changing business conditions is the driving ... direction continually varies because of changing customer requirements, competitors’ actions, or other occurrences in the business environment On this journey, the business objective rests on the...
Ngày tải lên: 28/06/2014, 08:20
The Strategy GapLeveraging Technology to Execute Winning StrategiesMichael Coveney Dennis potx
... hence, their strategic goals so they can act early to bring the tactical plan back on target Technology clearly has a role to play in facilitating this communication Failure to effectively communicate ... advance can be selected or corrections to the existing plan can be made to avoid or exploit the impact of any variances The ability to recognize and exploit changing business conditions is the driving ... direction continually varies because of changing customer requirements, competitors’ actions, or other occurrences in the business environment On this journey, the business objective rests on the...
Ngày tải lên: 28/06/2014, 22:20
Báo cáo khoa học: "The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin" pptx
... the binding of LigBCen and LigBCtv to Fn, Fig The binding of LigBCen or LigBCtv to the MDCK cells reduced leptospiral adhesion (A) Binding of LigBCen or LigBCtv to the MDCK cells Various concentrations ... added to each well and incubated for h at 37 C (Fig 3C) To determine the binding inhibition of Leptospira to the MDCK cells by LigBCen or LigBCtv by CLSM, the MDCK cells (10 ) were preincubated ... epifluorescence microscopy (EPM) and confocal laser-scanning microscopy (CLSM) To measure the binding of Leptospira to Fn by EPM, Leptospira (10 ) were added to each well (eight well culture slides) coated...
Ngày tải lên: 07/08/2014, 20:23
Báo cáo khoa hoc:" Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule" pptx
... not in the extracellular media Thus, the granulysin-GFP fusion construct correctly directs the biosynthesis of the chimeric molecule into the secretory pathway A previous publication demonstrated ... two color immunofluorescent confocal microscopy using a polyclonal antisera reactive to granulysin and a monoclonal antibody reactive to perforin, a well characterized constituent of cytolytic ... indicating that the chimera is altered in its subcellular distribution in comparison to the native molecule Conclusions to be drawn from these data regarding the mechanism(s) of sorting to cytolytic...
Ngày tải lên: 11/08/2014, 08:20
Báo cáo y học: " Role of the C-terminal domain of the HIV-1 glycoprotein in cell-to-cell viral transmission between T lymphocytes" potx
... cellular receptors (CD4, coreceptor) and cytoskeletal proteins (F-actin, talin) typically accumulate at the target cell contact [12,14-18] Even though some host cell signalling cascades that ... infected cells was subjected to intracellular p24 FACS to establish the percentage infected cells The total cell densities and the percentages of cells infected in the respective cultures were equalised ... http://www.retrovirology.com/content/7/1/43 Such polarisation includes accumulation of the viral structural proteins Gag and Env as well as the microtubule organising centre (MTOC) at the donor cell contact, while cellular...
Ngày tải lên: 12/08/2014, 23:23
Báo cáo y học: "Contribution of the C-terminal region within the catalytic core domain of HIV-1 integrase to yeast lethality, chromatin binding and viral replication" doc
... 5'ACTGACGCTCTCGCACCCATCTCTCTC-3' (nt position 329) PCR products were visualized in 1% agarose gel 10 11 12 Competing interests The authors declare that they have no competing interests 13 Authors' contributions ... partially complemented by the IN class I catalytic mutant D64E These results highlight the importance of the C- terminal region of the catalytic core domain of HIV-1 IN in its association with the chromatin ... amino acid E152, another crucial residue important for IN catalytic activity, did not disrupt IN's lethal activity in yeast cells [30] To test whether the IN catalytic mutants could induce the lethal...
Ngày tải lên: 13/08/2014, 05:21
head first iphone development a learners guide to creating objective c applications for the iphone 3 phần 2 ppsx
... dispatch events Interface Builder lists which events a component can trigger We need to attach the right component event to the code We wrote an action method earlier that we can connect the button ... how you click a button on the iPhone, the actual click inside the button isn’t what matters: it’s when you remove your finger (touch up) that the actual tap occurs Connecting an event to a method ... variable can point to a specific object in the nib (like a text field or something), and code (yes, probably your code) can use me to change the UI, set a text field’s content, change colors, etc Oh—I...
Ngày tải lên: 14/08/2014, 20:21
head first iphone development a learners guide to creating objective c applications for the iphone 3 phần 3 potx
... connect the outlet to the code Connect the picker to our outlet You’re probably expecting this by now! Back into Interface Builder to make the connection from the UIPickerView to the IBOutlet ... reference to the picker to get the actual values Mike selects We need to reimplement the sendButtonTapped method to pull the values from the picker Looking at the UIPickerView documentation, the ... iPhone by TuMuch Monee”]; 104 Chapter Reference count will be because alloc automatically sets count to “second” will have a retain count of after this block of code: from the alloc, from inserting...
Ngày tải lên: 14/08/2014, 20:21