cfu f assay data acquisition and analysis

Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc

Ngày tải lên : 30/03/2014, 04:20

... growth factor combination [HGF and ﬁbroblast growth factors and (FGF1 and FGF4)] to induce mouse ES cells to develop into functional hepatocytes These factors, named HIFC (hepatic induction factor ... hepatic induction factors (a combination of HGF, FGF1 and FGF4) were clearly up-regulated in the injured mouse liver Using a modiﬁed HIFC differentiation system, human AT-MSCs can be differentiated ... A B D C Fig Hepatic differentiation of human AT-MSC (A) Schematic illustration outlining the differentiation protocol The CD105+ fraction was isolated from whole fraction of AT-MSCs of using...

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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... a final concentration of μg/mL Cell culture media was changed the day after transfection with fresh culture media and green fluorescence was identified in cytoplasm of cells 48 h after transfection ... passage of ASCs expressed CD44, CD90 and CD105, and were negative for CD14, CD34, CD45 (Fig 3) Fig Flow cytometric analysis of surface-marker expression on ASC The seventh passage of ASCs expressed ... Kruskal-Wallis analysis for Olby scores and oneway ANOVA for SEP values and Luxol fast blue positive areas were used p-value ＜ 0.05 was considered significant Results Differentiation test of ASCs Adipogenic...

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Investigation of osteogenic characteristics of human adipose derived stromal cells

Ngày tải lên : 08/11/2015, 17:01

... member of ATF5, ATF4 is an important regulator of osteogenic differentiation in non-osteogenic cell lines To further understand the role of ATF5 gene, ATF5 was silenced with RNAi and its effect ... specific markers for osteogenesis Fig 4.11 B1 and B2 show the two different isoforms of osteopontin obtained As a control ß-actin (Fig 4.11 C) and hFOBs cell line (Fig 4.11 D) were used Fig 5.1 ... Biological and molecular mechanisms of osteogenic differentiation Bone formation has been thought to occur by lineage specific differentiation of a pool of precursor cells, which under the influence of...

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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx

Ngày tải lên : 10/08/2014, 10:20

... part of the cells (Figure 4A) The size of lipid droplets increased after two weeks, and most of the differentiated cells showed red lipid droplet throughout the cytoplasm (Figure 4B) After induction ... evaluated for markers of endothelial differentiation Yang et al Journal of Biomedical Science 2011, 18:59 http://www.jbiomedsci.com/content/18/1/59 Page of Figure Adipogenic differentiation of hADSCs ... but the purity of isolated hADSCs is not high and the methods for identification have not been fully developed Therefore, developing high efficient methods to isolate and identify hADSCs would...

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Characterization of human adipose tissue derived adult multipotent precursor cells

Ngày tải lên : 12/09/2015, 09:55

... A et al., 2001) The frequency of CFU- F and CFU- AP of ADSPC is in the order of in 100 – some 500-fold more than that found in marrow (Fraser JK et al., 2006) ADSC and MSC both possess the ability ... fixation was ineffective and must be replaced Vascularized bone autografts, had been used to enhance the biologic environment and fill bone defects Unfortunately, a finite amount of bone graft ... mature and healthy state Scaffolds must provide sufficient initial mechanical strength and stiffness to substitute for the mechanical function of the diseased or damaged tissue with the aim of repair...

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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf

Ngày tải lên : 13/08/2014, 13:20

... cocktail at 25°C for 25 Thereafter, 45 ml of PBS containing 2% FBS was added to dilute the buffy coat After gentle mixing, we layered 30 ml of the diluted buffy coat on top of 15 ml of Ficoll Hypaque ... phosphatase) Analysis of PLAP activity on day demonstrated transduction efficiency of > 80% (Fig 1B) DC functions through surface receptor signaling To see if LVs affected DC surface marker expression, ... staining (ICCS) and flow cytometry (FACS) analysis The d5 DCs were transduced by LV-PLAP and 48 hr later, analyzed by PLAP enzyme assay (B) FACS analysis of DC surface markers after viral transduction...

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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps

Ngày tải lên : 14/08/2014, 08:21

... specifically cleaves insulin-like growth factor binding protein (IGFBP)-4 and IGFBP-5 Proteolysis of IGFBPs regulates the bioavailability of IGFs, and because of the association between IGF levels ... in data analysis and design SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB ... University of Aarhus, Denmark, for his gift of antisera against the PAPPA protein and the staff of the Biology Technology Facility, University of York Apoptosis assay Unselected cells were treated for...

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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys

Ngày tải lên : 09/09/2015, 10:19

... fibroblast growth factor (FGF)-2, insulin-like growth factor (IGF)-1, hepatocyte growth factor (HGF), TGF-β1, and platelet-derived growth factor (PDGF-A and PDGF-B) In addition, primers amplifying genes ... affect HPTC, is helpful for improving the performance of this cell type in in vitro applications The central role of TGF-β1 and its antagonists in regulating HPTC performance was further confirmed ... Effects of BMP-7 and BMP-2 on HPTC 67 4.3.1 Effects of BMP-7 on the maintenance of epithelia formed by HPTC 67 4.3.2 Effects of BMP-2 treatment 70 4.3.3 Quantification of α-SMA...

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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY

Ngày tải lên : 09/09/2015, 11:18

... as the amount of enzyme causing the hydrolysis of µmol of p-nitrophenyl phosphate per minute at pH 9.8 and 25 oC (glycine buffer) dfddbFGF encapsulated in the scaffolds and bFGF provided as a ... understand the hepatic potential of ADSCs in 3D 10 scaffolds and thus more such studies need to be performed in scaffolds made of various biomaterials Such differentiated hepatocyte-scaffold constructs ... (n=3); % and $ represents P

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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx

Ngày tải lên : 10/08/2014, 23:21

... growth factors including TGFb, IGF, FGF, and PDGF A literature review of the data on the uses of PRP showed that it has a positive effect on the stimulation of bones and blood vessels and chondrocytes ... factor (TGF)b, insulin-like growth factor (IGF), fibroblast growth factor (FGF), and platelet-derived growth factor (PDGF) A literature review of the data on PRP shows that it has a positive effect ... the tropic effects of ADSCs on the existing tissues Further, various elements of the local environment can affect the differentiation of MSCs [6] Also, it is believed that a scaffolding material...

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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx

Ngày tải lên : 18/06/2014, 19:20

... generated from combined FLT3L and TGFb treatment (Figure 5B) These data suggest that FLT3L and TGFb are present and sufficient for CD11b+ MDSC induction, but technical difficulties in abolishing FLT3L, ... with FACS buffer, and resuspended in FACS buffer for analysis For intracellular staining, cells were fixed and permeabilized using Fixation/Permeabilization Kit (eBioscience, San Diego, CA) after ... Samples were run on a BD FACSCalibur flow cytometer and data acquisition and analysis were performed as above Data are from three unique donors and expressed as a fraction of labeled cells within...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... selected for embryo transfer or cryopreservation Our work confirms the importance of identifying factors that facilitate growth and inhibit differentiation of ESCs Liberation from a feeder cell ... group and the single blastomere culture group were disaggregated from their respective blastocysts and re-plated on to fresh feeder cells for confirmation and further analysis; no cells were frozen ... Baart EB, Van Opstal D, Los FJ, Fauser BC, Martini E: Fluorescence in situ hybridization analysis of two blastomeres from day frozen-thawed embryos followed by analysis of the remaining embryo on...

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Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model

Ngày tải lên : 11/09/2015, 10:02

... maturation……………………….………… 121 Figure 6.1 CLEC graft as a monolayer of cells on a PET membrane scaffold………127 Figure 6.2 Detection of CLEC-DsRed2 fluorescent clusters……………………… 129 Figure 6.3 Vascularization of transplanted ... CLECs from graft……………………………………………137 Figure 6.7 Survival of CLECs and keratinocytes in co-transplantation…………… 139 Figure 6.8 Detection of CLMC-eGFP fluorescent clusters………………………… 141 Figure ... EF elongation factor eGFP enhanced green fluorescent protein ELISA enzyme-linked immunosorbent assay ESC embryonic stem cell FACS fluorescence-activated cell sorting FBS fetal bovine serum FCS...

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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx

Ngày tải lên : 18/06/2014, 15:20

... after mesodermal induction for osteogenic, adipogenic, and chondrogenic differentiation The multilineage differentiation was performed for independent lineages of htMSCs, and no evident difference ... expansion, and differentiation into muscle, fat, cartilage, and bone of a new rich source of mesenchymal progenitor cells from normal adult hFTs Tissue fragments of hFTs, which are usually discarded after ... color Myogenic Differentiation For myogenic differentiation hFTs cells were cultured in myogenic differentiation medium consisting of 50% induction medium and 50% fresh DMEM /F- 12 (Invitrogen,...

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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx

Ngày tải lên : 09/08/2014, 10:23

... present in the IPFP tissue of fibroblast growth factor-2 (FGF-2) expansion Chondrogenic cultures of infrapatellar fat pad (IPFP) cells and effects of fibroblast growth factor-2 (FGF-2) expansion ... was unaffected by FGF-2 and was maintained in extended culture fat pad (IPFP) cells Cell surface characterisation for 3G5 in clonally expanded infrapatellar fat pad (IPFP) cells Cell surface staining ... diverse and sometimes paradoxical effects on cell proliferation and differentiation which are cell-type-dependent [29] This highlights the need for caution in extrapolating the effects of FGF-2 from...

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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf

Ngày tải lên : 13/08/2014, 05:22

... infection by HIV GFP The relative efficiency of infection of the clones is visually illustrated in Figure trates the results of a qPCR analysis for HIV-1 in genomic DNA of parental and mutant cells ... different between the parental and mutant cells To examine if the defect in infection was in the early stages of the life-cycle we next examined the extent of integration of HIV-1 DNA after infection ... we quantified HIV DNA 36 hours after infection in cytoplasmic and nuclear extracts of parental and 42-7 cells This analysis (Fig 6D) reveals up to a fold difference in the accumulation of HIV DNA...

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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"

Ngày tải lên : 25/10/2012, 11:10

... effect of time on loading efficiency and loading process was done for the previous concentrations for different times (15, 30, 60, 120 minutes) and compare the results [24] Study the effect of ... centrifugation for at 5000 rpm [22] Study the effect of concentration To determine the effect of drug concentration on loading efficiency we use different drug concentrations (2 mg, mg, mg, and ... determine differences between mean values * Significantly different according to time at p < 0.01, # significantly different according to concentration at p < 0.01 Table 2, Effect of pravastatin...

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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

Ngày tải lên : 19/02/2014, 02:20

... St Louis, MO, USA) and · of the provided buffer All PCR reactions were performed with one cycle of 94 °C for 60 s, followed by 45 cycles of 15 s at 94 °C and 60 s at 60 °C FEBS Journal 274 (2007) ... S Ruggieri for helpful comments and suggestions and T Begley (Department of Chemistry and Chemical Biology, Cornell University, New York, NY) for the gift of the plasmid pKLC100 References Bender ... removal of EDTA and addition of metal-ions Figure shows the hydrophobicity proﬁles of human and P ﬂuorescens ACMSD, performed according to Kyte and Doolittle [37] By comparing the mean A Fig Hydrophobicity...

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