cellular molecular markers and mechanisms underlying implantation

... hundred base pairs and VNTR, for variations in the number of tandem repeats (Tab I) The molecular techniques used for genotyping will be adapted to the variation type and to the scale and throughput ... different species by using exactly the same reagents and conditions However, the drawback is that the markers are generally dominant and generated at random The dominance problem can be partially overcome ... electrophoresis 4.2.2 Single strand DNA conformation and heteroduplexes Single strand conformation polymorphism (SSCP) is based on the specificity of folding conformation of single stranded DNA, when placed...

Ngày tải lên: 09/08/2014, 18:21

... carotenoid accumulation and flesh color co-localized with carotenoid-related genes: CMCRTR and BOH_1 in LG VI and CMBCYC and LYCB in LG VIII (Figures and 4), and as such become candidate genes for ... purified and recovered DNA was Sanger sequenced Proprietary programs selected sequences with SSRs and designed flanking primers Molecular markers A large proportion of molecular markers developed and/ ... additional markers and QTL Results and discussion Construction of the integrated map Anchor molecular markers Based on their previously observed even map distribution, polymorphism, and repeatability,...

Ngày tải lên: 11/08/2014, 11:21

... to study how pheromones influence innate behaviors and to understand the neuronal basis of pheromone perception The anatomical, cellular and molecular basis of chemosensory detection has been ... bulb cVA is a C-20 compound and is also known to function as a female aphrodisiac (Kurtovic et al., 2007), and is involved in aggression (Wang and Anderson, 2010) and aggregation (Bartelt et ... immature females and mated females are typically unreceptive and will exhibit different types of rejection behaviours (Connolly and Cook, 1973) These include running away from a male and kicking (exhibited...

Ngày tải lên: 09/09/2015, 08:15

... pyramidal cells (Blackstad and Kjaerheim, 1961; Andersen et al., 1966; Andersen et al., 1971) The MFs form distinct synapses with excitatory and inhibitory cells of the hilus and area CA3 It has been ... circuitry in relation to learning and memory formation requirements Furthermore, the molecular mechanisms underlying the MFs redistribution during learning and memory formation are still under ... synaptic protein rabphilin and Rab3A are required for the MFs LTP expression (Lonart et al., 1998a and 1998b) In summary, the biochemical mechanisms responsible for induction and expression of presynaptic...

Ngày tải lên: 09/09/2015, 18:55

... different molecular mechanisms involved in amygdala-related cuedor tone-dependent fear conditioning xi List of Tables Table 4.1 Average concentrations and standard errors of Fe Cu and Zn in different ... 110 5.1.3 Pharmacological studies on Cdk5 in learning and memory 112 5.1.4 Rational and objective .113 5.2 Materials and methods .113 5.2.1 Animals .114 ... training 115 5.2.3.2 Short-term contextual memory and cued memory test 115 5.2.3.3 Retention and extinction of long-term contextual memory and cued memory 116 5.2.4 Genotyping analysis...

Ngày tải lên: 09/09/2015, 18:55

... http://www.annals-general-psychiatry.com/content/4/1/8 MR GR NON-HANDLED NON-HANDLED HANDLED HANDLED 45 NO N HANDLED HANDLED 40 35 30 25 20 15 10 NUM R OF POSIT VE IVE CE L L S NUM R OF POSIT VE IVE CE L L S 45 NO N HANDLED HANDLED 40 35 30 ... http://www.annals-general-psychiatry.com/content/4/1/8 A 120 NO N HANDLED HANDLED 100 SECONDS 80 60 40 20 B NO N HANDLED HANDLED 25 SE CONDS 20 15 10 TARGET O PPO S ING Effects of handling on spatial learning and memory in the Morris ... NON HANDLED NON HANDLED HANDLED B HANDLED NUMBER OF POSITIVE CELLS 35 30 25 20 15 10 CA4 Figure handling on BDNF immunoreactivity in the CA4 region of the hippocampus Effect of3 Effect of handling...

Ngày tải lên: 08/08/2014, 21:20

... to pursue PhD and offering me the scholarship And last but not least, this dissertation is dedicated to my family My parents are always standing with me and giving me endless love and encouragement ... wife is always showing her patience and thoughtfulness to my work, and sharing the joy and happiness with me I feel fortunate and happy I am your son/husband Yours, Li Chunwei March, 2009, in ... reported profiles of cellular infiltration in Asian NP In this respect, this thesis focuses on the molecular mechanisms underlying the pathogenesis of Asian (especially Chinese) NP and its response...

Ngày tải lên: 10/09/2015, 15:49

... and Chang, 2002; Murthy and Wadsworth, 2005; Guha et al., 2005), and that actin assembly and disassembly are required for cytokinesis (O'Connell et al., 2001; Pelham and Chang, 2002; Murthy and ... Fluor 643 2.1.2.4 Goat (polyclonal) F-actin and DNA markers F-actin and DNA markers are listed in Table 27 1:100 1:100 1:100 1:100 Table 4: F-actin and DNA markers used in this study Final Name Source ... drugs used in this study: molecular mechanisms of action Name Molecular mechanisms of action Inhibit myosin II ATPase activity by binding to myosin II-ADP-Pi Blebbistatin and inhibit the release...

Ngày tải lên: 14/09/2015, 14:09

... Publication Lists Wei Z and Song J (2005) Molecular mechanism underlying the thermal stability and pH-induced unfolding of CHABII J Mol Biol 348 (1):205-18 Shi J., Wei Z and Song J (2004) Dissection ... at pH 6.3 and 293 K Proton Chemical Shifts of CHABII at pH 4.0 and 293 K Proton Chemical Shifts of [Phe21]-CHABII at pH 6.3 and 293 K Proton Chemical Shifts of [Phe21]-CHABII at pH 4.0 and 293 ... understanding of the molten globule state or partially-folded proteins In the present study, we expressed and refolded the recombinant proteins of CHABII and its mutant [Phe21]-CHABII, and subsequently...

Ngày tải lên: 26/11/2015, 12:54

... cells In our EMSA analyses, aside from the shifted band that contained USF1 and USF2, we observed other shifted bands (X and Y) As bands X and Y were only partially inhibited by 500-fold excess ... 70Z/3, J558L and A20) and four mouse epithelial cells (Y1, L cells, LA-4 and MMT060562) (Table 1) In both human [8] and mouse SHP-1 [9], alternative transcripts (both longer and shorter than the ... a probe Lanes: and 5, no nuclear extracts present in the reaction mix; lanes and 6, two specific complexes (A and B) formed when the reaction mix contains both nuclear extracts and labeled probe...

Ngày tải lên: 08/03/2014, 23:20

... identify the many genetic and environmental components that are involved in the pathogenesis of the problem and to carefully study the underlying molecular, cellular, and hormonal mechanisms On the basis ... volume book Thus, Nutrition and Metabolism: Underlying Mechanisms and Clinical Consequences offers only an up-to-date and authoritative review of the major scientific and clinical aspects of the ... chapters Nutrition and Metabolism: Underlying Mechanisms and Clinical Consequences, edited by Christos S Mantzoros, MD is a very welcome addition to the Nutrition and Health Series and fully exemplifies...

Ngày tải lên: 22/03/2014, 19:20

... AND PHARMACOLOGICAL MECHANISMS UNDERLYING BEHAVIOUR EDITED B Y P B B R A D L E Y Department of Pharmacology (Preciinical), The Medical School, University of Birmingham, Birmingham (England) AND ... HEBB, AND SMALLMAN, N (1956) Intracellular distribution of choline acetylase J Physiol C B (Lond.), 134,385-392 HEBB, AND WHITIAKERV P (1958) Intracellular distributions of acetylcholine and choline ... Isolation and characterisation of membranes H W W AND from rat cerebral cortex In Drugs and Cholinergic Mechanisms in the CNS, E HEILBRONN A WINTER (Eds.), Res Inst of Natl Def., Stockholm Almqvist and...

Ngày tải lên: 29/03/2014, 13:20

... enhance glucose metabolism and inhibit gluconeogenesis in the liver, and can thus reverse hepatic steatosis and fibrosis [20] Here, we explored the effects and molecular mechanisms of GH on AFLD ... study, Y.Q., and Y.P.T.; study concepts and design: Y Q., and Y.P.T.; data acquisition/analysis/interpretation: Y Q and Y.P T., statistical analysis: Y Q.; obtained funding: Y Q., and Y.P.T.; ... group were weighed and then euthanized, at which time blood, liver tissue, and adipose tissues were collected The perirenal and epididymal fat pads were pooled (visceral fat, VF) and weighed using...

Ngày tải lên: 18/06/2014, 16:20

... interleukin-6 (IL-6) and Intercellular Adhesion Molecule-1 (ICAM-1) as markers of inflammatory response, and matrix metalloproteinase (MMP-9) and cellular fibronectin (cFn) as markers of blood brain ... and designed the research; analyzed and interpreted the data; performed statistical analysis, handled funding and supervision and drafted the manuscript RRG, DB, OM, have acquired, analyzed and ... onset and lower levels of the inflammatory biomarkers ICAM-1 and IL-6 at 24 hours Previous studies by our group have reported associations between serum levels of these inflammatory markers and...

Ngày tải lên: 18/06/2014, 19:20

... interleukin-6 (IL-6) and Intercellular Adhesion Molecule-1 (ICAM-1) as markers of inflammatory response, and matrix metalloproteinase (MMP-9) and cellular fibronectin (cFn) as markers of blood brain ... and designed the research; analyzed and interpreted the data; performed statistical analysis, handled funding and supervision and drafted the manuscript RRG, DB, OM, have acquired, analyzed and ... onset and lower levels of the inflammatory biomarkers ICAM-1 and IL-6 at 24 hours Previous studies by our group have reported associations between serum levels of these inflammatory markers and...

Ngày tải lên: 20/06/2014, 03:20

... Charnie stand), 12 bands among 419, allowing discrimination between sessile and pedunculate oaks Among these 12 bands, two (F14a and 174g) were present in small frequencies in pedunculate oak and in ... populations (Roberts and Hiorns, 1965; Elston, 1971) study of the hybridization and morphology of the hybrid forms in oaks is imporThe tant to understand the evolution of these taxa and of their genetic ... seeds and natural regeneration of the same stand (Bacilieri et al, 1994) This result confirms that hybridization occurs in nature and hybrids survive to the first stage of life Both in sessile and...

Ngày tải lên: 08/08/2014, 18:21

... polymorphic molecular markers [9, 10, 12] makes it possible to establish a linkage between these mutations and anonymous markers, in order to connect both the former “classical” map and the molecular ... uropygial gland, U , lavander plumage color, LAV , and ametapodia, M P , mutations would be the last one not to be connected with the molecular map The connection between molecular and classical ... extraction and pool preparation Blood was sampled from paternal grand-parents, sire, dams and all progeny Individual extraction of high molecular weight DNA was performed according to standard procedures...

Ngày tải lên: 09/08/2014, 18:21

... bacteraemia and carcinoma of the colon and infective endocarditis is biotype-specific It was shown that there is 94% association between S bovis biotype I bacteraemia and infective endocarditis and 71% ... bacteraemia and colonic carcinoma while it is only 18% association between S bovis biotype II bacteraemia and infective endocarditis and 17% association between S bovis biotype II bacteraemia and colonic ... discovered and suggested mechanisms underlying the etiological association of S bovis/gallolyticus (SBG) bacteria with promoting, propagating, or initiating colorectal tumors, bacteremia, and endocarditis...

Ngày tải lên: 10/08/2014, 10:20

... for in vitro biomolecular profiling of cancer biomarkers, in vivo tumor imaging, and dual-functionality tumor-targeted imaging and drug delivery [7] Early detection of cancer and targeted drug ... nm, 15 mW) and detected through a 461/50 nm bandpass filter Samples were scanned in 0.5 μm steps and saved both as cytometric data and as PMT-reconstructed images Data was acquired and analyzed ... http://www.jnanobiotechnology.com/content/8/1/13 Page of QD cellular uptake and intracellular translocation process Figure Cellular uptake of QDs in human mammary non-tumorigenic MCF-10A cells and carcinoma MCF-7 cells The...

Ngày tải lên: 11/08/2014, 00:22

... conceived and designed the study and the website hosting the results JMJG also performed the assemblies, SNP mining, evolutionary analysis and molecular marker design and essays JNM conceived and participated ... amplifications we found 87% (220 of 261 LxH and 193 of 210 LxP) of the molecular markers to work as predicted It is worth noting that 10% of the successful markers showed bands corresponding to both alleles ... unigenes and perform gene evolution analysis and tests for positive selection We were able to suggest candidate genes and gene families that may be related to domestication of the cultivated tomato and/ or...

Ngày tải lên: 12/08/2014, 03:20