cellular effects of lipoic acid and its role in aging

... v-KIND KIND2 domain contain the MAP2-binding core site (A) Division of the KIND2 domain (456–620) into five subregions: KIND2-1 (amino acids 456–487), KIND2-2 (amino acids 456–521), KIND2-3 (amino ... and functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across a range of ... in the KIND2 domain of v-KIND contain the core MAP2 binding module To identify the core MAP2-binding site within the KIND2 domain (amino acids 456–620), we analyzed five EGFP-fused KIND2 domain...

Ngày tải lên: 14/02/2014, 19:20

... examined first [20] We observed that the intracellular calcium level increased in OccWT-overexpressing cells, and that 2-APB inhibited the effects of OccWT Therefore, the increase in intracellular ... mitochondria-dependent apoptosis and reduction of invasion, along with downregulation of MMP expression, we postulated that increased intracellular calcium might be involved in the effects of occludin In previous ... explain the induction of apoptosis and reduction of invasiveness mediated by occludin In previous reports, intracellular calcium concentrations were reported to in uence the onset of apoptosis and...

Ngày tải lên: 18/02/2014, 18:20

... stage of tetramerization Possibly, loss of copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain ... important in the brain, therefore the role of copper binding or loss of its binding could be related to specific cerebellar function(s) of stefin B [18], which remains to be seen by more in vivo ... effect of copper binding on the secondary structure was determined using CD in the far UV To prepare proteins without Cu2+, part of each protein 4252 Fig Comparison of copper binding by stefin A and...

Ngày tải lên: 19/02/2014, 06:20

... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important role in leucine catabolism AUH was overproduced in E coli and characterized by measuring ... (c.719C>T) in exon and an insertion mutation c.613–614insA This insertion causes a frameshift that starts at Met205 and leads to the introduction of a stop codon after four amino acids The intra-assay ... reaction of leucine catabolism at the protein and DNA levels and developed a novel assay for enzyme analysis in a diagnostic setting The human AUH protein was first recognized by its ability to bind...

Ngày tải lên: 19/02/2014, 07:20

... were incubated at 37 °C in a humidified 5% CO2 incubator in the absence (control) and presence of increasing concentrations of inhibitors for 10 days, and the number of molting larvae was determined ... constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations of PPi (0.05–0.5 ... concerning the kinetics and properties of the enzyme More strikingly, we show a novel role of the PPase enzyme in the development and molting process of A suum larvae in vitro constructed in UniZap...

Ngày tải lên: 20/02/2014, 11:20

... protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction between VBARP and Nef is not due to the lack of input proteins ... and domain distribution of VBARP-L translated amino acid sequences The grayshade regions in the amino acid sequence of VBARP-L protein represent the presence of ankyrin (ANK) repeats, amino acids ... host cellular functions including apoptosis in infected and uninfected bystander target cells through its interaction with host cellular proteins [22–25] However, it is not clear at this point...

Ngày tải lên: 07/03/2014, 21:20

... N-terminal domain of the polypeptide of each of these organisms is followed by a stretch of hydrophobic amino acids that is presumably involved in the protein’s anchoring to the membrane Also in ... retainment of newly synthesized proteins in the cytosol due to the inability to import matrix proteins by the fraction of growing and dividing glycosomes The miscompartmentation of PTS-1 and ... membrane-spanning domains in PEX14 have been unambiguously identified and it was observed in mammalian cells and S cerevisiae that both the N-terminal and the C-terminal domain are at the cytosolic face of...

Ngày tải lên: 23/03/2014, 17:21

... Biochem 269) To understand the role of MF in the growth and reproduction of crustaceans, considerable effort has been invested in studying the regulation of the biosynthesis of MF [1,4,6] Although ... During the injections and subsequent handling of the animal, principles of laboratory animal care and specific national laws were followed The rFAMeT used for the second and third booster injections ... NaCl/Pi for and incubated in the peroxidase substrate solution containing 0.01% H2O2 and 1.39 mM 3,3¢-diaminobenzidine (Sigma, St Louis, MO, USA) The colour reaction was terminated by rinsing the...

Ngày tải lên: 31/03/2014, 21:21

... acid deposition and Mg supply of the soil (Kaupenjohann, 1989) It is interesting to note that an increase of the acid load of the same soil (Fig 2) caused a higher release of both Mg and Ca into ... leaching, acidification of the tree’s rhizosphere, changes in soil chemistry and plant nutrition In addition to the proton deposition, N inputs have to be taken into account when investigating influences ... symptoms of needle yellowing and dieback of spruce stands in this region Furthermore, proton load, particularly of the canopy, decreases Ca uptake into roots There is no indication of an imbalancing...

Ngày tải lên: 09/08/2014, 04:20

... molecular chaperones in the folding of oxidized proteins Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein J Biol Chem 2001, 276:21337-21342 ... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... adrenomedullin and adrenomedullin-binding protein-1 in sepsis: down-regulation of proinflammatory cytokines Crit Care Med 2002, 30:2729-2735 Darby NJ, Creighton TE: Functional properties of the individual...

Ngày tải lên: 13/08/2014, 11:22

... metastasis in vivo The N-terminal fibronectin (FN) binding domain of TG2 (tTG 1-140), lacking both enzymatic and GTPase function, induced EMT in OC cells, suggesting the interaction with FN involved in ... 50% inhibitory concentration IF Immunofluorescence IFN-γ Interferon-gamma IGFBP-3 Insulin-like growth factor-binding protein-3 IκBα Inhibitor of kappa B alpha ILK Integrin-linked kinase IP3 Inositol ... reacts with a lysine-containing protein or peptide to cause crosslinking of the substrates TG2 reacts with a polyamine to cause amine incorporation into the glutamine-containing protein or peptide...

Ngày tải lên: 24/08/2014, 10:58

... predominantly in erythroid cells HRI is regulated by heme through the two heme-binding regions in HRI: an N-terminal domain of HRI and in an insert region in the protein kinase domain of HRI ... involved in amino acid biosynthesis and the uptake and salvaging of nutrients (67) In mammals, the related ATF4 is induced in response to a broader spectrum of stresses, leading to activation of genes ... cytoplasmic splicing of mRNA encoding the Xbox binding protein1 (XBP1) in mammals and homologue of the mammalian activating transcription factor/cAMP-response element (CRE)-binding protein (HAC1) in yeast...

Ngày tải lên: 24/08/2014, 12:32

... translated amino acid sequences of GGT proteins in different H pylori strains possessing different level of GGT activities Determining the subcellular localization of GGT in H pylori Examining the ... Sialic acid- binding adhesin The sialic acid- binding adhesin is a 70 kDa protein that belongs to the large Hop family of H pylori outer membrane protein, including BabA sabA is found to be present in ... of ammonia and indirectly by urease-induced stimulation of the inflammatory response, including recruitment of leukocytes and triggering of the oxidative burst in neutrophils Survey of Literature...

Ngày tải lên: 12/09/2015, 09:55

... important role in myelin formation and maintenance in oligodendrocytes 26 Aims and rationale of current work 1.3 Aims and rationale of current work One of the main research focuses of the lab ... brains were minced into sizes of about 1mm3 and digested in 0.25% of typsin/EDTA and 25 g/mL of DNaseI (Sigma: D5025), at 37oC for 10 minutes Typically, four-six brains would be harvested and ... digested in a total volume of 2mL of 0.25% of trypsin/EDTA and 25 g/mL of DNaseI After enzymatic digestion, the mixture of trypsin/EDTA and DNaseI would be removed and replaced with 2mL of plating...

Ngày tải lên: 12/09/2015, 09:55

... According to the type of amino acid phosphorylated, the protein kinases can be divided into two subgroups: protein serine/threonine kinases and protein tyrosine kinases Serine/threonine protein kinases ... discovered kinases that catalyze the phosphorylation of hydroxyamino acids All of the known protein kinases including tyrosine kinases share a related catalytic domain of about 260 amino acids and are ... domain of about 260 amino acids just like serine/threonine kinases A number of residues within this domain are highly conserved between both types of protein kinases, but the tyrosine kinases and...

Ngày tải lên: 12/09/2015, 21:10

... location of BGT-1 has to be addressed at protein level, in order to gain a thorough understanding of its function in the brain BGT-1 is capable of transporting both betaine and GABA, as examined in ... oocytes and various cell lines But the physiological substrate of BGT-1 in the brain remains to be determined Of particular interest is the elucidation of the role of BGT-1 in excitotoxic brain injury, ... decarboxylation of cysteine sulfinate by cysteine sulfinate decarboxylase (Foos and Wu, 2002; Tappaz, 2004) The role of taurine in osmoregulation in the brain has been well established Efflux of taurine in...

Ngày tải lên: 16/09/2015, 15:55

... critical role in regulating lead contamination in drinking water, a precise and fast method for its detection is required to determine its abundance in drinking water sample and assess its bioavailability ... solids, and (3) explore the stability of PbO2 in chloraminated water in the presence of Br- to advance our understanding of the role of PbO2 in lead release in drinking water Firstly, a modified iodometric ... change of disinfectant from free chlorine to monochlorine has been shown to alter the stability of PbO2 and trigger high levels of lead released into drinking water Lin and Valentine [21] investigated...

Ngày tải lên: 30/09/2015, 06:15

... expressing PLA2 isoform in the brain (Molloy et al., 1998; Yang et al., 1999b; Yang et al., 1999a) and its protein expression decreases during aging (Aid and Bosetti, 2007) It is present in all brain ... crucially involved in effecting the role of pancreatic sPLA2 1.7 Aims of the present study The above findings suggest an important role of members of the PLA2 family in neuronal plasticity and / ... hippocampus including CA1-3 fields, dentate gyrus and brainstem (Shirai and Ito, 2004) The significance of PLA2 in maintaining membrane lipid homeostasis of the brain is evident in all these...

Ngày tải lên: 04/10/2015, 17:04

... persistent and non-inundation) and types of inundation (rainy, stagnant-water, heavy-rainy, monsoon-flood and drainage-water inundations) Ten subdistricts were selected by integrating information ... defecating in open field Households with children defecating in room Households with children defecating in verandah Households with children defecating in latrine   defecating in latrine Households ... LMK PDL PHS PSM NCD Hand washing with soap before eating Hand washing without soap before eating No hand washing before eating Hand washing after defecation with soap Hand washing after defecation...

Ngày tải lên: 05/09/2013, 10:15

... transcript in the adult intestine and in the developing embryo, whereas Mxi1-SRb transcripts predominate in the adult liver and kidney [12] In our initial description of Mxi1-SRa and its comparison ... cellular processes including proliferation, apoptosis, differentiation, and so on Isoforms of numerous proteins have been studied and compared in this manner, including alternative isoforms of ... generated by introducing the WR cDNA containing the full 5¢-UTR in pcDNA3.1 The coding region of Mxi1-SRa and Mxi1-SRb were subcloned by PCR in a vector containing an amino terminal flag tag The...

Ngày tải lên: 18/02/2014, 16:20