cellular consequence of gag binding to pi 4 5 p2 and ps
Báo cáo y học: " Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: Novel insights into the regulation of Rev nuclear impo" doc
... transfected with 0 .5 μg of pCAGGSHA Rev, and/ or 0 .5 μg of pFLAG HIC, pFLAG-HIC(2 144 ), and/ or pFLAG-HIC( 144 - 246 ) 48 hours post-transfection, cells were fixed in 4% paraformaldehyde and permeabilised ... immunoprecipitates were analysed by Western-Blot (WB) to examine expression levels of Rev, HIC and its mutants, and co-immunoprecipitation of HIC, HIC ( 144 246 ) and HA-Rev, respectively Similar to previous ... pFLAG-HIC ( 144 - 246 ) Rev expression is shown in Red and HIC, HIC (2- 144 ) and HIC ( 144 - 246 ) expression is shown in Green Nuclei were counterstained with DAPI (Blue) Representative images of transfected...
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Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
... Sci USA 89, 58 9 59 3 49 Cheng, L & Kelly, T.J (1989) Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro Cell 59 , 54 1 55 1 50 Sock, E., ... the cellular restriction of virus propagation [ 54 ] Factors involved in this regulation might be cellular proteins such as puralpha, p53, or alternative splicing products of viral proteins [51 ,55 ,56 ] ... a-primase lacking TAg or vice versa; lanes and 4, 0.2 U and 0 .4 U of human; lanes and 6, 0.2 U and 0 .4 U of murine DNA polymerase a-primase The approximate sizes of the reaction products are indicated...
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Báo cáo Y học: A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter ppt
... Planta 203, 44 2 44 7 Liu, J & Zhu, J.K (1997) An Arabidopsis mutant that requires increased calcium for potassium nutrition and salt tolerance Proc Natl Acad Sci USA 94, 149 60– 149 64 43 Medina, ... shows the binding of the promoter with the p40 protein and not with PsCCaMK (Fig 7) The absence of binding with PsCCaMK could be due either to the fact that it does not bind to DNA at all, to a weak ... presence of 50 mM NaCl and eluted either using a salt gradient of 0. 05 1 M containing 0 .5% TritonX-100 and 10 mM 2-mercaptoethanol or with low pH glycine buffer (pH 2.9) Under both of these sets of...
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Báo cáo khoa học: RNA reprogramming of a-mannosidase mRNA sequences in vitro by myxomycete group IC1 and IE ribozymes pptx
... in this study Name 5 - to 3¢ sequence OP299 OP479 OP483 OP 757 OP 758 OP 759 OP760 OP761 OP762 OP763 OP7 64 OP7 65 OP766 OP816 OP887 OP888 OP889 OP890 OP891 OP892 OP893 OP8 94 OP8 95 OP896 OP897 OP898 ... Authors Journal compilation ª 2006 FEBS T Fiskaa et al RNA reprogramming of a-mannosidase mRNA sequences DiGIR2, Fse.L569, and Fse.L1898 are 81%, 79% and 1 04% for U1 357 , and 39%, 58 % and 96% for U1381, ... respect to the Tth.L19 25 ribozyme activity (100%) The raw yields of trans-spliced target RNA for the Tetrahymena ribozyme were found to vary from to 15% and from to 8% for the sites U1 357 and U1381,...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... stimulated with LPS, we examined the effects of inhibitors of PKA and PKC on TS-enhanced NO production As shown in Fig 5A, the PKA inhibitors KT -57 20 and H8 and the PKC inhibitors Ro31-2880 and GF109203X ... findings to ours They proposed that TS-induced apoptosis in hepatopoietic and cancer cell lines is caused by the prevention of PKC activity due to activation of PP2A, similar to a-T activation of PP2A ... Induction of cancer cell apoptosis by a-tocopheryl succinate: molecular pathways and structural requirements FASEB J 15, 40 3 41 5 14 You, H., Yu, W., Sanders, B.G & Kline, K (2001) RRR-aTocopheryl...
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Báo cáo khoa học: Nck-1 selectively modulates eIF2aSer51 phosphorylation by a subset of eIF2a-kinases docx
... medium and transformed with different individual plasmids (p425GAL1, p425GAL1-Nck-1, p426GAL1-PKR, p413GAL1-PKR) or cotransformed with p426GAL1-PKR and p425GAL1-Nck-1 or p413GAL1-PKR and p425GAL1-Nck-1 ... Nck-1 + - % of peIF2α / total eIF2α (Vector=100%) GCN2Δ WT A + peIF2αSer51 eIF2α Nck 140 120 100 80 60 40 20 Vector Nck WT + p425GAL1 B WT + p425GAL1-Nck-1 GCN2Δ + p425GAL1 Glucose Galactose +3AT ... previously [36], to generate p425GAL1-Nck-1 and p426GAL1-PKR p413GAL1 (generous gift of B Turcotte, McGill University, Montreal, Canada), a low copy number vector compared with p425GAL1 and p426GAL1,...
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Báo cáo Y học: Barley a-amylase Met53 situated at the high-affinity subsite )2 belongs to a substrate binding motif in the bfia loop 2 of the catalytic (b/a)8-barrel and is critical for activity and substrate specificity pot
... 11 43 17 11 12 34 24 19 18 16 48 17 67 167 167 667 667 667 833 50 00 PNPG5 Wild-type M53E M53A M53S M53G M53D M53Y M53Wa G G G G G PNP 14 44 41 32 53 15 40 44 16 48 35 16 40 44 16 39 46 15 ... GH77 47 63 46 62 6883 748 9 7186 6883 50 72 90113 6992 96119 1111 35 202217 48 66 78 95 78 95 812830 255 2 85 841 09 841 01 6896 1 952 14 47 749 4 200217 196213 57 75 557 3 6381 236 253 41 60 257 286 118 144 48 67 ... Modulation of 53 90 H Mori et al (Eur J Biochem 269) 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 activity and substrate binding modes by single and double subsites +1/+2 and )5/ )6 mutation of barley...
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Báo cáo hóa học: " Supporting QoS in MANET by a Fuzzy Priority Scheduler and Performance Analysis with Multicast Routing Protocols" docx
... End -to- end delay (s) 0.0 25 0.0 15 0.01 0.0 15 0.01 0.0 05 20 25 30 35 40 45 0.0 05 50 20 Number of senders NTPMR ODMRP CAMP 25 30 35 40 Number of senders 45 50 With FPS Without FPS Figure 11: Delay versus ... 2. 05 1. 75 2.3 2.2 2 .5 2. 25 3.2 2 .5 3.1 3. 15 2.9 2.3 3.0 3.0 FPS 1.9 1. 95 2.1 2. 25 2 .4 2. 95 FPS 2. 15 1.9 1. 85 2. 15 2. 35 2.1 Hence due to this, the dropping of packets experiencing multihops gets ... (packets/s) PS WHS RR GS 1.8 1.8 1. 75 1. 75 1.9 1. 95 1. 85 1. 85 1. 95 2.0 1.9 1. 95 2.1 2.2 2.1 2.1 2.3 2.3 2.3 2.3 2. 85 2.9 2.8 2. 75 Delay (s) PS WHS RR GS 2. 25 2. 25 2. 25 2. 25 2. 05 2.1 2.1 2.1 1.8 1.7 2.05...
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The Project Gutenberg EBook of Short Cuts in Figures, by A. Frederick Collins pdf
... 39 42 45 12 16 20 24 28 32 36 40 44 48 52 56 60 10 15 20 25 30 35 40 45 50 55 60 65 70 75 12 18 24 30 36 42 48 54 60 66 72 78 84 90 14 21 28 35 42 49 56 63 70 77 84 91 98 1 05 16 24 32 40 48 56 ... 72 84 96 108 120 132 144 156 168 180 12 13 26 39 52 65 78 91 1 04 117 130 143 156 169 182 1 95 13 14 28 42 56 70 84 98 112 126 140 1 54 168 182 196 210 14 15 30 45 60 75 90 1 05 120 1 35 150 1 65 180 ... 64 72 80 88 96 1 04 112 120 18 27 36 45 54 63 72 81 90 99 108 117 126 1 35 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 10 11 22 33 44 55 66 77 88 99 110 121 132 143 1 54 1 65 11 12 24 36 48 ...
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Ultrasonography in In Vitro FertilizationRoger A. PiersonDepartment of Obstetrics, Gynecology pptx
... tracking of the needles used for follicle aspiration was probably the single most important step in making IVF as safe and effective as it is today (Fig 3) ( 45 ,53 55 ,59 –63) Retrieval of oocytes ... Steril 19 85; 44 :1 95 199 19 Zegers-Hochschild F, Gomez Lira C, Parada M A comparative study of the follicular growth profile in conception and nonconception cycles Fertil Steril 19 84; 41 : 244 – 247 20 ... Gynecol 1987; 69: 6 65 668 53 Wikland M, Enk L, Hammarberg K Use of a vaginal transducer for oocyte retrieval in an IVF/ET program J Clin Ultrasound 1987; 15: 2 45 – 251 54 Wikland M, Enk L, Hammarberg...
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Báo cáo khoa học: "In vitro studies on the modification of low-dose hyper-radiosensitivity in prostate cancer cells by incubation with genistein and estradiol" pot
... Western-Blot analysis of p21 and actin in LNCaP after 24 h of hormonal incubation, irradiation with 0 .5 Gy and Gy Western-Blot analysis of p21 and actin in LNCaP after 24 h of hormonal incubation, ... 60% 40 % 20% 0% 60% 40 % 20% 0% 24 48 54 60 66 72 24 48 54 time [h] G0/G1-phase S-phase G2/M-phase G0/G1-phase 100% 66 72 S-phase G2/M-phase 100% 80% 60% cells [%] cells [%] Gy 60 time [h] 40 % ... 60 time [h] 40 % 20% 0% 24 48 54 60 66 72 50 % 0% 24 48 time [h] G0/G1-phase S-phase G2/M-phase G0/G1-phase 54 60 66 72 tim e [h] S-phase G2/M-phase Cell cycle distribution of PC-3 cells Figure Cell...
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Báo cáo y học: "Discovering and validating unknown phosphosites from p38 and HuR protein kinases in vitro by Phosphoproteomic and Bioinformatic tools" doc
... Journal of Clinical Bioinformatics 2011, 1:16 http://www.jclinbioinformatics.com/content/1/1/16 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 MAP kinase p38beta: different features of ... measurement of Ca Ca distances between residues E47(A)-K50(B) (red), S48(A)-S48(B) (blue) and E47(A)-K50(B) (green), during the MD trajectory Distortion of the HuR dimmer in the presence of Ser -48 (HUR ... residues (Glu47A-Lys50B, Ser48A-Ser48B and Lys50A-Glu48B) indicated a clear and irreversible displacement from the initial values during the first steps of the MD simulation (Figure 5C, upper plots)...
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Báo cáo khoa học:" In vitro host range, multiplication and virion forms of recombinant viruses obtained from co-infection in vitro with a vaccinia-vectored influenza vaccine and a naturally occurring cowpox virus isolate" pot
... 23 256 ± 18 244 ± 15 237 ± 16 2 35 ± 12 229 ± 14 240 ± 13 (0) 25 (50 ) ( 14) 18 (36) (16) ( 14) (6) 255 ± 28 272 ± 35 253 ± 19 251 ± 15 257 ± 11 257 ± 243 ± 29 2 64 ± 35 238 ± 18 238 ± 18 243 ± 243 ... Cryo-electron tomography of vaccinia virus Proc Natl Acad Sci USA 20 05, 102:2772-2777 http://www.virologyj.com/content/6/1 /55 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 Gallego-Gomez ... Res 20 04, 106:189-197 Page 12 of 13 (page number not for citation purposes) Virology Journal 2009, 6 :55 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 http://www.virologyj.com/content/6/1 /55 Smith...
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Báo cáo khoa học: " In vitro host range, multiplication and virion forms of recombinant viruses obtained from co-infection in vitro with a vaccinia-vectored influenza vaccine and a naturally occurring cowpox virus isolate" pps
... 23 256 ± 18 244 ± 15 237 ± 16 2 35 ± 12 229 ± 14 240 ± 13 (0) 25 (50 ) ( 14) 18 (36) (16) ( 14) (6) 255 ± 28 272 ± 35 253 ± 19 251 ± 15 257 ± 11 257 ± 243 ± 29 2 64 ± 35 238 ± 18 238 ± 18 243 ± 243 ... Cryo-electron tomography of vaccinia virus Proc Natl Acad Sci USA 20 05, 102:2772-2777 http://www.virologyj.com/content/6/1 /55 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 Gallego-Gomez ... Res 20 04, 106:189-197 Page 12 of 13 (page number not for citation purposes) Virology Journal 2009, 6 :55 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 http://www.virologyj.com/content/6/1 /55 Smith...
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Báo cáo y học: "Distribution of airway narrowing responses across generations and at branching points, assessed in vitro by anatomical optical coherence tomography" docx
... KCL 5 .4; MgSO4 1.2; NaHCO3 25; sodium morpholinopropane sulphonic acid buffer 5. 0; glucose 11 .5; and CaCl2 2 .5) at 37°C The preparation was stretched slightly to a length shown previously to approximate ... and NHMRC of Australia (No 40 39 15, 51 38 54 ) Salary for PBN was provided by an NHMRC Australian Based Biomedical Fellowship (No 51 3921), and for PRE by an NHMRC Senior Research Fellowship (No 51 37 04) ... Am Rev Respir Dis 1987, 1 35( 5):11 05- 1111 14 Bai Y, Zhang M, Sanderson MJ: Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways Am J Respir Cell Mol...
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USING BRAND AS AN EFFECTIVE WEAPON TO COMPETE IN THE MARKET: A CASE STUDY OF NHAT LINH COMPANY
... (Table 5. 2) Table 5. 1: Factors Affecting Consumers Choices of AVS Brand Factors N Mean Std Deviation Others 5. 0000 0000 Safety 181 4. 7 45 9 58 84 Reliability 183 4. 7 049 6633 Brand reputation 1 75 4. 5 54 3 ... - 45 6.1 Developing LiOA Brand Identity 45 6.1.1 The brand as a product 45 6.1.2 The Brand-as-organization 46 6.1.3 The Brand-as-person: Brand personality 46 6.1 .4 Band ... 3KVA NL-30,000W/3 30KVA NL -40 00 4KVA NL - 45 ,000W/3 45 KVA NL -50 00 5KVA NL- 75, 000W/3 75KVA NL- 750 0 7.5KVA NL-90,000W/3 90KVA NL-10000 10KVA NL- 150 ,000W/3 150 KVA NL- 150 00 15KVA NL-200,000W/3 200KVA...
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Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx
... an important vector in the transmission of cultural knowledge: they point to, and exploit, the shared cultural touchstones that speakers and listeners alike can use to construct and intuit meanings ... Poetic and Non-Poetic Simile: Structure, Semantics, Rhetoric Poetics Today, 14( 1) Douglas R Hofstadter 19 95 Fluid Concepts and Creative Analogies: Computer Models of the Fundamental Mechanisms of ... words and phrases which, when wrenched from their conventional contexts of use, are free to take on enhanced meanings and provide additional returns to the investor The realm in 15 which a maker of...
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Tài liệu In Rare Form A Pictorial History of Baseball Evangelist Billy Sunday pptx
... references and index isbn 0-87 7 45 - 959 -2 (pbk.) Sunday, Billy, 1862–19 35 States—Biography Evangelists—United Sunday, Billy, 1862–19 35 Homes and haunts I Title bv37 85. s8f57 20 05 269'.2'092—dc22 20 05 043 9 14 ... belonged to the Thompson family Two three-piece sets of Victorian furniture, both of which consist of two side chairs and a platform rocker, all came from the Thompson household The most elaborate of ... $1,000, and a total of $ 75, 000 was riding on the outcome of the race.12 Sunday had a change of heart after the wagering began and asked Anson to let him out of the arrangement, but Anson refused, and...
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Báo cáo khoa học: The pH dependence of kinetic isotope effects in monoamine oxidase A indicates stabilization of the neutral amine in the enzyme–substrate complex ppt
... 6 .5 7.0 7.2 7 .5 8.0 8 .5 9.0 9.2 9 .5 – 0. 042 0. 047 – 0. 041 0. 044 0. 0 45 0.082 – 0.261 0.213 0.1 34 0.136 0.138 0.0 34 0.033 0.039 0.079 0 .42 4 0.019 0. 1 45 – 0.122 0.078 0. 048 0.028 0. 042 0.268 38 54 ... 4. 0 3 .5 3.0 2 .5 2.0 1 .5 1.0 0 .5 0.0 6.0 6 .5 7.0 7 .5 8.0 8 .5 9.0 9 .5 10.0 6.0 6 .5 7.0 7 .5 8.0 8 .5 9.0 9 .5 10.0 pH D 140 120 100 80 60 40 20 -20 6.0 6 .5 7.0 7 .5 8.0 8 .5 9.0 9 .5 10.0 kred/Ks (s–1 ... half-reaction of benzylamine oxidation exhibited FEBS Journal 2 75 (2008) 3 850 –3 858 ª 2008 The Authors Journal compilation ª 2008 FEBS 3 851 A B 3.0 kcat (s–1) 2 .5 2.0 1 .5 1.0 0 .5 40 20 10 4. 0 3 .5 3.0 2.5...
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