cells in vitro differentiation and applications

... ,etahpsohp lorecylg -β fo detsisnoc taht muidem noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna ... ecnanetniam eht dna euqinhcet reporp eht ,erutluc yramirp ot gnilpmas morf emit eht taht thguoht saw tI ]72,22[ srotinegorp citeiopotameah rof esac eht sa emas eht ,srotinegorp lamyhcnesem ni ... ladiobuc wohs ton did sCSM BCU eht ,sCSM worram enob ekilnu ,revewoH )4 giF( )gniniats assoK-noV yb deifitnedi( laitnetop cinegoetso evah sCSM taht gnitseggus ,etitapayxordyh demrof sCSM BCU ehT enosahtemaxed...

Ngày tải lên: 07/08/2014, 18:21

... performance in applications in kidney tissue engineering and in vitro toxicology Identifying such factors would also help to understand the cross-talk between HPTC and endothelial cells, which ... change during passaging, and cells become increasingly senescent Furthermore, dedifferentiation or transdifferentiation processes can occur during the in vitro culture of primary cells (50, 54, 57-59) ... sensitivity of the cells, and thus, thorough characterization of the cells is essential for applications in in vitro toxicology and kidney tissue engineering 17 One of the most important applications...

Ngày tải lên: 09/09/2015, 10:19

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... 1995;The spatial and temporal expression of the alpha beta integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis Differentiation...

Ngày tải lên: 11/09/2015, 09:16

... and HepG2/ADM cells The drug-resistant HepG2/ADM cells were cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing ... mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study The nude mice inoculated with HepG2/ADM cells were divided into four groups with seven mice in each ... HepG2/ADM cells were washed with PBS and fixed in 100% methanol for 10 Cell monolayers were blocked in 5% BSA in PBS for 45 and incubated for h at room temperature with P-gp antibodies (Invitrogen,...

Ngày tải lên: 21/06/2014, 03:20

... nanoparticles in electroanalysis became an area of research which is continually expanding Within both the trend towards the miniaturisation of electrodes and the ever-increasing progress in preparation and ... Reactions in RDE (Rotatingof Electrochemical Reactions in RDE (Rotating Disk Electrode) and IRDE (Inverted Rotating Disk Electrode) Based Reactors Modeling and Quantication Disk Electrode) and IRDE (Inverted ... Reactions in RDE (Rotatingof Electrochemical Reactions in RDE (Rotating Disk Electrode) and IRDE (Inverted Rotating Disk Electrode) Based Reactors Modeling and Quantication Disk Electrode) and IRDE (Inverted...

Ngày tải lên: 28/06/2014, 17:20

... NY) containing 10% (v/v) heat-inactivated fetal bovine serum (Gibco, Grand Island, NY), penicillin (100 U/mL) and streptomycin (100 mg/mL), and they were maintained in a 37°C humidified incubator ... Apoptosis induction by isoproterenol or propranolol in combination with irradiation in BGC-823 and SGC-7901 cells There was an increasing rate of apoptosis in gastric cancer cell lines in response ... examined using flow cytometric (FCM) analysis with Annexin V-PI staining After propranolol pre-treatment (50 μM for 24 hr) and following irradiation, FCM demonstrated an increase in Annexin-V...

Ngày tải lên: 09/08/2014, 09:20

... cell line Washing and incubation with HFM was done in the maintenance cultures After 24 h cells were detached using trypsin/EDTA, counted, diluted and replated in 25 cm2 flasks in FMH + goserelin ... induced by goserelin incubation and irradiation in LNCaP cells In PC-3 cells, we found more expression of this protein after incubation with goserelin than after irradiation alone Goserelin incubation ... brown staining Changes in protein expression To test the functional activity of goserelin in the investigated cell lines, we analyzed protein expression and phosphorylation that are involved in functional...

Ngày tải lên: 09/08/2014, 10:21

... assay and GEM assay experiments, assisted in data analysis and interpretation and in writing and revising the manuscript SP and JG carried out the Western blot experiments CH and SA assisted in ... are involved in transferring electrons to and from NAD(P)H in normal and neoplastic hepatocytes [26] Moreover, DCPIP has demonstrated anticancer activity against human melanoma cells in vitro and ... proangiogenic and pro-inflammatory factors, VEGF, COX-2, IL-6 and IL-8, in cultured human colon cancer cells; (c) DCPIP and DCPIP NPs are similarly effective in inhibiting VEGF-induced angiogenesis in ovo;...

Ngày tải lên: 11/08/2014, 00:22

... number of cells detaching was measured in the cell supernatant Cells were grown in 24 well plates at a seeding density of 105 cells/ ml in complete media overnight; cells were washed in PBS and treated ... activation and inhibition on TNFα-induced injury and apoptosis in human intestinal epithelial cells Br J Pharmacol 2003, 140:41-52 Roberts NJ, Jones AC, Whelan CJ: Apoptosis in two cell lines after ... using the method described in the Cytochrome C release kit (Oncogene) Cells were grown in 75 cm2 flasks at a seeding density of × 10 cells in complete media overnight Cells were washed in PBS and...

Ngày tải lên: 11/08/2014, 08:21

... contribute to insulin resistance and atherosclerosis [34,35] Thrombin activation of adipose cells may contribute to the circulating inflammatory cytokines and insulin resistance found in obese individuals ... http://www.journal-inflammation.com/content/6/1/4 5000 Thrombin Thrombin Thrombin 1U 10 U + Lepirudin * 4000 ‡ 3000 2000 1000 PBS Thrombin Thrombin Thrombin 1U 10 U + Lepirudin PBS Thrombin Thrombin Thrombin ... Thrombin Thrombin Thrombin 1U + Lepirudin 10 U Thrombin Thrombin Thrombin 10 U + Lepirudin 1U 70000 6000 PBS PBS Adipose Tissue IL-6 pg/ml 4000 1000 Thrombin Thrombin Thrombin 10 U + Lepirudin 1U...

Ngày tải lên: 11/08/2014, 08:22

... nine of the genes down-regulated in response to seasonal H1N1 influenza virus (but not pdmH1N1) encode zinc finger proteins, including ZNF175 ZNF175 contains 13 zinc fingers and a KRAB domain, ... inoculum in the mock infected cells The cells were incubated with the virus inoculum for h in a water-jacketed 37°C incubator with 5% CO Then the cells were rinsed times with warm PBS and replenished ... functions of individual candidates in influenza pathogenesis will require further investigation Lack of the control of transcriptional suppression by zinc finger proteins in pdmH1N1 infected cells We...

Ngày tải lên: 12/08/2014, 11:22

... processing was cathepsin H [53,61] and inhibition of cathepsin H results in decreased production of mature SP-B Napsin A and pepsinogen C, asparatic proteases, are also involved in the processing ... IL-13 increases the intracellular SP-D protein level when compared to untreated cells in rat neonatal ATII cells in vitro [35] We also performed similar experiments in vitro with rat adult ATII cells ... detected in the lung epithelium, interstitium, and in mononuclear cells in surgical lung biopsies from patients with IPF [59] IL-13 is elevated after administration of bleomycin in murine lungs and...

Ngày tải lên: 12/08/2014, 11:23

... the distinctive staining of specific epithelial cells (dark brown) and the lack of staining in underlying cells and surrounding alveolar regions in both mRNAs was ultimately similar, induction ... Such increases in gene expression could play a role in regulating the cumulative increase in intracellular Muc2 and Muc5ac observed in these cells following exposure to pro-inflammatory cytokines ... determined whether C4 and 45M1 exhibitted differential staining in mucous cell-containing tissues, and whether C4 could distinguish differentially between sections from murine small intestine and...

Ngày tải lên: 12/08/2014, 16:20

... 7:39 turn, recruit eosinophils via the production of inflammatory mediators, including cytokines (IL-4 and IL-5) and chemokines (eotaxin) [4-7] The eosinophils upon activation and recruitment cause ... Pseudomonas aeruginosa-induced mucin overproduction in epithelial cells Proc Natl Acad Sci U S A 1998, 95:5718-5723 Steinke JW, Borish L: Th2 cytokines and asthma Interleukin-4: its role in the pathogenesis ... carcinoma cell line NCIH650 (ATCC, Manassas, VA) was cultured in serum free ACL-4 media supplemented with mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin and 0.02 mg/ml insulin Cells...

Ngày tải lên: 12/08/2014, 16:20

... medium containing glutamine, penicillin and streptomycin After washing with ice-cold PBS, stimulated cells were lysed with reducing sample buffer and incubated for 10 minutes on ice Proteins were ... were obtained after incubating cells with GSH (1.25 – mM) instead of NAC (data not shown) In contrast, in NCI-H292 cells but not in PBEC, depleting GSH using BSO resulted in a full inhibition ... buthionine sulphoximine (BSO) on CSC-induced epithelial wound closure in NCI-H292 and in PBEC Effect of buthionine sulphoximine (BSO) on CSC-induced epithelial wound closure in NCI-H292 and in PBEC...

Ngày tải lên: 12/08/2014, 18:20

... in vivo in xenograft tumors, observing a highly significant number of the same genes being regulated by E2 in vivo as well as in vitro Our findings of good agreement between the in vivo and in ... (clusters B and F); transcripts induced or repressed through 24 hours beginning at intermediate time points (around hours; clusters C and G); and transcripts induced or repressed beginning at later ... from in vitro models represents dynamic information, where cells can be readily manipulated and the effects observed Cells can be manipulated to a certain extent in vivo, for instance using the...

Ngày tải lên: 14/08/2014, 16:21

... containing large spherical mitochondria which, in the living state in vitro, are continually elongating, retracting, and reorienting themselves, leading to a wide variety of cell shapes (Steinman ... vaccination against infectious diseases Human CD8 T -cells can be distinguished into naive, effector and memory T -cells based on the expression of CCR7 and CD45RA; naive CD8 T -cells are CCR7+ and ... antigen and is followed by a contraction phase, when 90–95% of the effector cells die in the ensuing days and weeks and the remaining 5–10% become long-lived memory cells (Harty and Badovinac,...

Ngày tải lên: 09/09/2015, 18:58

... releasing human insulin (Kang, Kim et al 2009) The isolated cells were differentiated into insulin producing cells after a 2-stage differentiation protocol, including activin, nicotinamide, and/ or ... to induce differentiation into insulin producing cells in a chemical-defined culture system (Zhang, Jiang et al 2009) The differentiated cells comprised nearly 25% insulin-positive cells and ... nestin expression in insulin producing cells differentiation still remained to be elucidated Two reports 14 showed that the insulin expression of the cells derived from nestin-expressing cells...

Ngày tải lên: 09/10/2015, 11:18

... Summaries Series | Management accounting in networks: Techniques and applications Findings 4.1 Field study findings The main findings from the field study indicate that the relationships with ... elements being to: Present a generalisable framework for describing and understanding management accounting and control patterns in networked organisations Introduce new concepts and techniques ... partner, or to exploit linkages with the partner clients Conclusions In presenting a generalised framework for describing and understanding management accounting and control patterns in networked organisations,...

Ngày tải lên: 09/02/2014, 21:12

... with increasing grain size, and fine-graining transferring information with decreasing grain size Sometimes, upscaling and downscaling refer specifically to coarse-graining and fine-graining, ... done, finding the indefinite integrals of nonlinear models, in general, is a formidable task If the closed-form indefinite integrals cannot be found, approximating the double definite integral ... (e.g., grain, extent, coverage, spacing) To put the concept of scaling into action, one has to invoke specific scaling operations (e.g., extrapolation, coarse-graining, fine-graining, interpolation)...

Ngày tải lên: 17/02/2014, 19:20