cell surface aggregation phenomena

... measured in NaF-treated intact cells results from the cleavage of the disaccharide at the cell surface by an extracellular ‘acid trehalase’ pool [14] The cell- surface localization accounts for ... place in this subcellular compartment In contrast, targeting this enzyme at the cell surface is indispensable for growth of yeast cells on trehalose Ath1 domains relevant for cell- surface targeting ... found at the cell surface Controversy concerning the localization of Ath1 has been raised in two recent papers In a previous study, we suggested a localization for Ath1 at the cell surface based...

Ngày tải lên: 18/02/2014, 06:20

... the surface of a variety of cell lines [42,48,52,55,56] and on the surface of endothelial cells [57,58] To address whether nucleo4674 lin is also present on the surface of developing muscle cells, ... the surface of myotubes (arrow in Fig 6D), as well as on the surface of nonmyosinexpressing cells Some of the punctate localization also occurred at the cell cell interface between these cells ... and incubated with the cells for h at room temperature Cells were washed, mounted and analyzed as described above To semipermeabilize cells for intracellular staining, cells were incubated in...

Ngày tải lên: 19/02/2014, 05:20

... Sertoli cell cultures (2525 ‹ 257 and 2538 ‹ 246 ng per well in three different cell cultures) Cell surface HSPG are bFGF partners during Sertoli cell postnatal development Developing Sertoli cells ... In conclusion, bFGF requires cell surface HSPG for the inhibition of steroidogenesis in developing Sertoli cells Cell surface HSPG are bFGF targets during Sertoli cell postnatal development As ... Sertoli cells Our present study demonstrates that bFGF in¯uences this expression in 20-day-old-rat Sertoli cells During testicular development, the physiology of Sertoli cells is modi®ed The cell...

Ngày tải lên: 21/02/2014, 03:20

... membrane to the nucleus 1.2 Signal Processing Cell communications with other cells and the extracellular matrix involve cellsurface proteins, such as receptors, cell adhesion molecules, ion carriers, ... any multicellular organism, the body’s cell communicate Cell signaling enables the coordination of cell activities, from basic life to tissue development and remodeling These basic cellular activities ... hand, surface signaling, signaling during endocytosis into the receiving cell and receptor recycling for further delayed signaling are crucial steps of cell signaling In polarized cells, the cell...

Ngày tải lên: 05/03/2014, 22:21

... and cell- surface expression but is unable to regulate cellular iron uptake like the wild-type HFE protein [4,15] Although much insight into HFE function has been gained through studying the cellular ... number of cells displaying this ERC/punctate pattern simultaneously displayed reticular localization in the same cells (50 ± 2% of N110A-expressing cells, 50 ± 2% of N130A-expressing cells and ... of these cells simultaneously displaying reticular localization (55 ± 7% of NN110/130AA-expressing cells, 51 ± 4% of NN130/234AA-expressing cells and 67 ± 7% of NN234/110AA-expressing cells, n...

Ngày tải lên: 06/03/2014, 22:21

... the surfaces of endothelial cells The presence of the F1 b-subunit in retinoblastoma Y-79 cell surfaces is consistent with previously reported expression of F1Fo-ATP synthase in tumor cell surfaces ... synthase on HUVEC surfaces, leading to inhibition of migration and proliferation of endothelial cells [30–32] HUVECs possess high ATP synthesis activity on the cell surface [33] Extracellular ATP ... a protein(s) at the surface of endothelial cells To determine whether the endothelial PEDF-binding component was related to cell- surface F1Fo-ATP synthase, we prepared subcellular fractions of...

Ngày tải lên: 22/03/2014, 21:21

... number of cell surface receptors was assessed by binding of an anti-LDL-R monoclonal Ig (IgGC7) at °C A 1-h treatment of HepG2 cells with PMA resulted in a 30% decrease in the number of cell surface ... mean decrease in cell surface receptor number following PMA treatment was also seen in CHO cells transfected with human LDL-R (CHO-715); however, in these cells the number of surface receptors ... overexpression Furthermore, the cell surface cleavage mechanism appeared more sensitive to PMA regulation in HSF than in CHO cells Enzymes responsible for ectodomain shedding of cell surface proteins have...

Ngày tải lên: 23/03/2014, 12:20

... binding the cell- surface expressed nucleolin leading to the inhibition of HIV-attachment to the cell surface PTN binding to cells involves high and low affinitybinding sites even in cells that ... a receptor is the cell surface- expressed nucleolin Accordingly, by confocal immunofluorescence laser microscopy, we show that cell- surface bound PTN colocalizes with that of surface- expressed ... that PTN binding and internalization in cells is mediated by the cell- surface nucleolin Cross-linking of surface- bound PTN results in the clustering of surface nucleolin In general, the cross-linking...

Ngày tải lên: 30/03/2014, 20:20

... demonstrating reduced cell surface expression of CXCR4 in HIV-1 infected RH9 cells Immunofluorescence microscopy demonstrating reduced cell surface expression of CXCR4 in HIV-1 infected RH9 cells Panel ... reactivity (Fig 2A, B), all control cells expressed CXCR4 The CXCR4-specific MAb displayed cell surface membrane fluorescence in 100% of mock-infected cells (Fig 2C, D) Most cells in the HIV-1-infected ... Jurakt cells Panel E and G: CXCR4 staining of induced Jurkat cells The fluorescent cell in panel G is representative of a minor population of cells in the induced culture (

Ngày tải lên: 20/06/2014, 01:20

... also transported to the cell surface of SKI-1/S1P deficient cells (data not shown) The finding that only an endo H-sensitive form of Lassa virus GP-C was found at the cell surface suggests that ... sensitivity of cell surface transported Lassa virus Figure glycoprotein Glycosidase sensitivity of cell surface transported Lassa virus glycoprotein At 48 h post-transfection, Vero cells expressing ... post-transfection, cells were surface labelled with biotin Following cell lysis and immunoprecipitation using α-GP-2, samples were subjected to SDS-PAGE and blotted to nitrocellulose Surface biotin-labelled...

Ngày tải lên: 20/06/2014, 01:20

... cytometry analysis of cells Either isolated cells from OC were directly used for flow cytometric analysis or cells were used after adherence and cultivation, as described above Cells were washed ... using CELLQuest software (Becton Dickinson, Heidelberg, Germany) Dead cells were excluded by propidium iodide (Sigma) staining Cells were gated on forward and side scatter to exclude debris and cell ... Fluorescence-activated cell sorting For cell sorting, native isolated cells from OC were stained with saturating concentrations of CD9-FITC, CD90-allophycocyanine and CD166-PE Single cells were sorted...

Ngày tải lên: 09/08/2014, 01:23

... extracellular protein interactions Genome Res 2008, 18:622-630 Karaulanov EE, Bottcher RT, Niehrs C: A role for fibronectin-leucine-rich transmembrane cell- surface proteins in homotypic cell ... recognition events mediated through cell surface proteins technically challenging The aim of this study was to identify novel receptor interactions that are involved in neural cellular recognition events, ... expressed receptors, there are several other families of cell surface proteins that contribute to neural recognition processes A comprehensive extracellular network of interactions within the developing...

Ngày tải lên: 09/08/2014, 20:20

... virus and the cell surface The options to use unpurified viruses, low MOI and cell numbers indicate the opportunity to study virus host cell interaction even when the amount of cells and viruses ... TCID50 /cell) , the ability to study interaction using a limited amount of cells was also investigated A decreasing number of cells were incubated with CVB5 at a concentration of MOI 0.05 TCID50 /cell ... TCID50 /cell, was incubatedwith 2.5 × 105 cells and bound virus was analysed with RT-PCR B) Decreasing number of CHO and HeLa cells, 2.5 × 105-2.5 × 102, were incubated with MOI 0.05 TCID50/cell...

Ngày tải lên: 12/08/2014, 04:21

... inhibitor-treated E36 cells or Lec8 cells The human-derived XMV, XMRV, shows the PMV-like restriction of AKR6 in hamster cells; XMRV does not infect Lec8 cells or inhibitor-treated E36 cells (Tables ... CasE#1 efficiently infected M dunni cells (Xpr1sxv) and M pahari cells (Xpr1p) as well as rat and mink cells, but failed to infect hamster cells, NIH 3T3 (Xpr1n) and cells carrying Xpr1c (Table 2) ... infectivity on mouse cells carrying the variants of Xpr1 (Table 2) Both viruses infected NIH 3T3 (Xpr1n) and cells carrying Xpr1sxv, but did not infect cells of M pahari (Xpr1p) or cells carrying Xpr1c...

Ngày tải lên: 12/08/2014, 23:22

... also been linked to the downregulation of cell surface CD4 in primary CD4+ T-lymphocytes [14] This was confirmed in the SupT1 cell line, in which cell surface CD4 downregulation by Nef of unintegrated ... apoptosis, modulate viral transcription, and alter cellular chemotaxis in infected cells [39,40] Downregulation of cell surface CD4 by Nef in primary CD4+ T-cells by unintegrated DNA is well established ... downregulates cell surface CXCR4, CCR5 and CD4 expression on Rev-CEM cells The Rev-CEM cell line was derived by transducing the Rev and Tat dependent GFP vector pNL-RRE(SA) [18] into CEM-SS cells, resulting...

Ngày tải lên: 12/08/2014, 23:23

... conservation of unique cell surface antigen mosaics in cryopreserved PBMCs from HIV+ individuals Here, we have used an antibody microarray constructed on the surface of a nitrocellulose coated slide ... simultaneously analyze 135 different cell surface antigens (128 cluster of differentiation antigens plus other surface antigens) on peripheral blood CD4+ and CD8+ T cell subsets from HIV+ and HIV- ... expression of cell membrane receptors for cytokines and chemokines were more pronounced in CD4+ T cells Results The pairwise comparisons of groups identified 17 statistically differential cell surface...

Ngày tải lên: 13/08/2014, 05:22

... to HAART, cell surface expression of adhesion molecules and levels of circulating adhesion molecules requires further investigation Three cell surface antigens were associated with cell activation ... and its expression on CD8+ T cells identifies the mature cytolytic effector cells [37,38] CD57 expression on CD8+, CD4+ T cell and NK cells is a general marker of cell proliferative inability ... CD28changes in the PBMC cell surface antigens in HIV patients on HAART: (A) CD2, CD3epsilon, CD5 and CD95; (B) Time related and CD36 Time related changes in the PBMC cell surface antigens in HIV...

Ngày tải lên: 13/08/2014, 06:20

... score Furthermore, we evaluated the cell surface expression previously [31] and the oxidative metabolism in monocytes In the present study, the expression of cell surface receptors and ROS generation ... characterize the cell population of neutrophils and monocytes This approach avoids cellular stimulation, known to occur in cell isolation processes, while providing a good gating for both cell populations ... positive cells All events were acquired and stored Neutrophil analyses were performed using forward- and side-scatter parameters combined with CD66b positive and CD14 negative stained cells The surface...

Ngày tải lên: 13/08/2014, 10:20

... the extracellular matrix In mammals, some integrins are limited to certain cell types or tissues: αIIbβ3 to platelets; α6β4 to keratinocytes; αEβ7 to T cells, dendritic cells and mast cells in ... signals from within the cell [1] They serve as transmembrane links between extracellular contacts (other cells or the extracellular matrix) and the actin microfilaments of the cytoskeleton, whose behavior ... function Upon binding an extracellular ligand, integrins generate an intracellular signal and, conversely, their functioning can be regulated by signals from within the cell [1] They serve as transmembrane...

Ngày tải lên: 14/08/2014, 07:21

... Introduction 1.1 Overview of Cell Surface Structures The mechanics of the cell surface plays an important role in the morphological changes of the cells when the cells carry out biological processes ... of the cell surface structures during the biological process that involves significant morphological changes The model consists of two parts: The first one is the mechanics of the cell surface ... transported to different organelles of the cell such as endoplasmic reticulum (ER), Golgi apparatus and mitochondria Similar to the cell surface, the surfaces of these organelles are also made...

Ngày tải lên: 10/09/2015, 08:36