cell lines and their use in published research

... as the metal binding event would be totally independent of the interactions involved in the enzyme–substrate complex The binding of MUG and Mg2+ are not independent, however, but instead are highly ... donor involved in the transferase Fig Diagram showing the thermodynamic equilibria and their dissociation constants involved in the binding of UDP-Glc, MUG and Mg2+ to TcdA Values shown in parentheses ... spectroscopic handles that can be used to probe directly the Mg2+ binding to TcdA [29] Mg2+ binding to free TcdA is invisible to our assays Thus, in this study, we have resorted to looking at the...

Ngày tải lên: 08/03/2014, 22:20

... herefrom, that: 11 In polar coordinates, the sine-wave is represented in intensity and phase by a vector o c, and in combining or dissolving sine-wa/aes, they are to be combined or dissolved by ... space, and of which the complex quantity in a plane is only a special case M B STKINIHTTZ: In introducing j , first as distinguishing index and then defining it as V — my object was to introduce ... end of line, that is line grounded, and Line of infinite length are evidently of little practical interest, but of importance is only the case of a line feeding into an inductive or non-inductive...

Ngày tải lên: 04/06/2014, 12:33

... indicators and their use in sustainable agriculture and rural development 19 Land quality indicators and their use in sustainable agriculture and rural development 19 The context of indicators in FAO ... issues and participatory approaches Land quality indicators and their use in sustainable agriculture and rural development 11 CONCEPTS, DEFINITIONS AND PRINCIPLES "Land", the "functions of land", ... examples of improvements in land quality (e.g., results of soil conservation and land -use planning case studies); Land quality indicators and their use in sustainable agriculture and rural development...

Ngày tải lên: 06/07/2014, 08:20

... families for genotyping, selecting recombination events in recurrent backcrosses, using advanced intercross lines (AIL) or performing linkage disequilibrium and haplotype-based studies in outbread populations ... differences in the techniques used in different laboratories are minor ones, principally concerning the use or not of an automatic sequencing machine for size determination For SNP genotyping, this ... just using SNPs in animal genetics 277 standard oligonucleotides in combination (in addition to restriction enzymes in the case of AFLPs), considerably reducing the effort and consumables, and therefore...

Ngày tải lên: 09/08/2014, 18:21

... 2001) On the other hand, lapatinib can bind to the ATP-binding pocket of EGFR/HER2 protein kinase domain preventing self-phosphorylation, which in turn, resulting in the inhibition of subsequent ... into a wide range of intracellular responses MAPKs (Mitogen-activated protein kinases) are protein Ser/Thr kinases that can initiate signaling cascades in response to extracellular stimuli, and ... staining Cell cycle phase can be differentiated by measuring their DNA content, where G1 phase is 2n, S phase is between 2n and 4n, and G2 phase is 4n PI can diffuse into cell nucleus and binds...

Ngày tải lên: 10/09/2015, 09:09

... target, and the selection of aptamers with both fast and slow binding kinetics It also can be used to accurately determine the binding affinities, kinetics and specificity of aptamer sequences In ... nanomolar binding affinity and specificity towards leptin was found In the second project the use of non-SELEX to select aptamers which bind to human hemoglobin and bovine catalase protein was achieved ... the use aptamers as affinity probes in ACE include IgE, HIV type reverse transcriptase, thrombin and antithrombin III12-14 The use of aptamers in biosensors is becoming a huge area of interest in...

Ngày tải lên: 10/09/2015, 09:22

... hydrophobins Fig (Continued) in the knockout mutant hfbI –, only the respective mass peak was missing (Fig 3B) Deviating post-translational processing of hydrophobins in H jecorina strains To investigate ... hydrophobins HFBIII of H jecorina and T longibrachiatum, and SRH1 and HFB1 of T atroviride, involving signal peptides, Kex2type processing, and C-terminal amino acid cleavage (Table 2) In addition, the recorded ... H jecorina strain QM 9414 (D), and the mutant strains QM 9414 Dhfb1 (B) and QM 9414 Dhfb1Dhfb2 (A) HFB I and HFB II indicate the processed hydrophobins Whereas the HFB I peak is missing in the...

Ngày tải lên: 19/02/2014, 02:20

... included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) and maintained in our laboratory ... serum-supplemented L-15 (Gibco, Grand Island, NY, USA); SK-BR-3 cells were maintained in 10% fetal bovine serum-supplemented RPMI-1640 (Hyclone); T-47D and BT-474 cells were maintained in RPMI-1640 (Hyclone) ... USA) and MCF-7 cells were maintained in 10% fetal bovine serum-supplemented Dulbecco’s modified Eagle’s medium (DMEM) (Hyclone, Logan, UT, USA); MDA-MB-231 cells were maintained in 10% fetal bovine...

Ngày tải lên: 22/03/2014, 21:20

... CPPs and their cargo [7,14,24] Interestingly, most investigations involving CPPs have been carried out in mammalian cell lines and there are only few reports on translocation of CPPs in plant cells ... the combined use of cell permeabilizing agent and cell- penetrating peptides results in the delivery of such large sized cargoes in plant cells involving endocytic ⁄ macropinocytic pathways In conclusion, ... proteins by arginine-rich intracellular delivery (AID) and Tat-protein transduction domain has been reported in onion and corn root tip cells [28,29] Cationic oligopeptides such as polyarginine...

Ngày tải lên: 23/03/2014, 07:20

... Short interfering RNA (siRNA) transfection Curcumin suppressed cell growth and induced G1/S cell cycle arrest in both DNR-insensitive and -sensitive AML cell lines KG1a and Kasumi-1 cells were seeded ... siRNA caused significant apoptosis, similar to that seen in curcumin-treated cells, suggesting an important role for Bcl-2 in curcumin-induced apoptosis in these CD34+AML cell lines Accumulating ... participated in its design and coordination DX contributed effort in designing, performing, and analyzing the results and conclusion All authors read and approved the final manuscript Competing interests...

Ngày tải lên: 18/06/2014, 19:20

... /n 3.1 In this section, we obtain the application in the algebraic Riccati equation of our results including 3.1 Some of our results and 3.1 cannot contain each other Theorem 3.1 If 1/p and K ... Majorization and Its Applications, vol 143 of Mathematics in Science and Engineering, Academic Press, New York, NY, USA, 1979 17 C.-L Wang, “On development of inverses of the Cauchy and Holder inequalities,” ... Combine 3.11 and 3.13 , we obtain cp,q n2−1/q n i 1/p p λi R tr K − 2tr K λ n A − tr Q ≤ 3.14 Solving 3.14 for tr K yields the right-hand side of the inequality 3.2 Similarly, we can obtain the...

Ngày tải lên: 22/06/2014, 02:20

... Biosciences), which absorb and emit in higher wavelength light (ex/em, 774/789 nm), and investigated their applicability in cell labeling and in vivo imaging Results and discussions Preparation ... applied to in vitro cell labeling and in vivo imaging Methods Cells, animals and chemicals The RAW264.7 cell line was purchased from the China Center for Type Culture Collection, Chinese Academy ... Odyssey Infrared Imaging System Cell labeling with IRDy800CW-MNPs To investigate the labeling of cells with nanoparticles, RAW264.7 cells were seeded into cell culture plates and cultured in DMEM...

Ngày tải lên: 11/08/2014, 00:22

... waters are also monitored In this chapter, drinking water sources and the situation of drinking water supply in the Delta is presented Water using practice for drinking and selected pesticide residues ... pesticides directly by breathing in the chemicals while spraying or indirectly by drinking contaminated water or consuming foods products such as vegetables and fishes containing pesticide residues ... grown in all of the fields during the two cropping seasons, and these fields are fallow during flooding periods In the winter - spring crop, soil often experienced puddling before rice sowing when...

Ngày tải lên: 26/11/2014, 14:31

... live cell bioimaging In the first application for live cell bioimaging, a protein of interest having an Nterminal cysteine was expressed inside a live cell using intein-mediated protein splicing ... domain, 2) mini inteins, lacking the endonuclease domain, 3) trans-splicing inteins, where the splicing junctions are not covalently linked and 4) Alanine inteins, where alanine is the N-terminal ... multicolor cell labeling and imaging Proteins labeled with BIOTIN may be used to study protein-protein interactions by in vivo experiments utilizing biotin-avidin binding Probe C2FL may be used for...

Ngày tải lên: 08/11/2015, 16:30

... grove from points Sampling points St.1 and St.2 are in the same stream, and sampling point St.3 is in the other stream The two streams flow together to be one stream and the sampling point St.4 is ... and periphery of the Seto Inland Sea The location of the research area and the distribution of the citrus groves in Matsuyama region are shown in Fig Matsuyama is situated in Shikoku Island in ... grove were investigated and their behaviors in the collecting reservoirs were discussed The main results obtained from the investigation are as follows: 1) Nitrogen concentration in grove soil...

Ngày tải lên: 05/09/2013, 10:17

... interesting things Learning idioms and proverbs is not an easy task, so students often get confusing in defining, memorizing and using them To overcome these, some solutions are introduced in chapter ... an insight into the similarities and differences in the use of language and images in English and Vietnamese idioms and proverbs II Some popular English idioms and proverbs relating to food and ... elephants, and American proverbs, many collected and published by Benjamin Franklin, are about hard work bringing success Proverbs are used to support arguments, to provide lessons and instruction, and...

Ngày tải lên: 11/12/2013, 23:48

... learning and using possessive determiners in English Errors in using “a” and “the” in possessive determiners In process of studying “possessive determiners”, Vietnamese learners made some misuses ... determiners use for feelings and thoughts to the person [81] Too many people blame their failings and their unhappiness on luck Scientists have taken a great interest in his ideas (Collin Cobuild, ... DETERMINERS IN ENGLISH I Possessive determiners with noun indicating relationship between the noun and the person involved in Object in sb’s possession or ownership Possessive determiners are used...

Ngày tải lên: 11/12/2013, 23:53

... metalloproteinases in vitro and eliminates production of avb3 integrin, a protein missing in normal melanocytes but often manufactured during the invasive phase [141] Hsp27 over expression in melanoma cells ... identified, and the role of ATP-dependent chaperones in substrate release and refolding revealed sHSPs operate in the front lines of cell defense, protecting proteins during stress and providing opportunities ... aB-Crystallin R120 corresponds to aA-crystallin R116 and both are conserved a-crystallin domain arginines R120G aB-crystallin permits intermediate filament self association in vitro, although binding...

Ngày tải lên: 19/02/2014, 18:20

... other known cysteine-containing chitin-binding domains [14], such as plant chitin-binding proteins and horseshoe crab antimicrobial peptides with chitin-binding affinity [15–17] Rebers and Willis reported ... chitin-binding proteins in T tridentatus Amino acid compositions of high molecular mass (HMM) chitin-binding proteins HMM chitin-binding proteins resolved by 2D SDS ⁄ PAGE were categorized into ... with asterisks Numbers on the right indicate amino acid residue numbers and DE29 is involved in chitin binding In insects, cuticular proteins containing cysteine residues have not been reported,...

Ngày tải lên: 07/03/2014, 21:20