cell fate proliferation and migratory itinerary

... expression of genes that are involved in cell cycle progression and cell fate specification of the NSCs and differentiated cells leading to increased neuro- and gliogenesis In addition, high glucose ... apoptosis and proliferation of NSCs and differentiated cells in high glucose environment···················································································79 2.3 Cell fate specification ... involved in the proliferation and cell fate specification of embryonic NSCs ······························································99 1.1 High glucose impairs the proliferation and survival...

Ngày tải lên: 30/09/2015, 06:36

... required for cell proliferation and cell growth or whether its essential role in proliferation is restricted to specific cell types Control of Cell Proliferation and Growth by Myc Proteins 331 ... targets of Myc in cell proliferation and their proposed functions Control of Cell Proliferation and Growth by Myc Proteins 333 2003) There is clear evidence that this effect is direct and not mediated ... both cell growth and cell proliferation through multiple pathways and that there is neither a single critical effector gene nor a small group of such genes In cell culture, Myc can affect cell...

Ngày tải lên: 25/10/2013, 21:20

... the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also showed ... not PARP-1, was required for SMC proliferation, and identified a novel link between mono-ADP-ribosylation and c-fos gene expression Materials and methods Materials Cell culture materials (DMEM, fetal ... electrophoresis in 1.7% agarose gels and visualized with SYBR Green I Data measurement and statistical analysis Radiotracer, cell number and enzyme assay data were quantified and plotted as means ± SEM...

Ngày tải lên: 17/03/2014, 09:20

... endogenous p53 and p21 expression and decreased endogenous C-myc expression (Figure 4C) Thus, it can be inferred that the inhibition of cell proliferation and cell cycle arrest in CK2a knockdown cells ... http://www.translational-medicine.com/content/9/1/97 Cell lines and culture conditions The human colorectal cancer cell lines LoVo, SW480, HT29, HCT116 and LS174T were maintained in RPMI 1640 (Gibco, Grand Island, NY, USA) supplemented ... CRC cell proliferation compared to the control (nonspecific siRNA) (F = 32.854, P < 0.01 for LoVo cells; F = 32.655, P < 0.01 for SW480 cells), and treatment with emodin markedly reduced proliferation...

Ngày tải lên: 18/06/2014, 19:20

... material and immune cell development involves in human carcinogenesis In this study, we studied about the toxic effects of borax on immune cell proliferation (lymphocyte proliferation) and genotoxicity ... the correlation between the immune cell proliferation and the borax cytotoxicity Results Studying the effect of borax on immune cell proliferation (lymphocyte proliferation) The cultures of human ... effectiveness to lymphocyte proliferation in this study It was toxic for cellular proliferation Borax effects lymphocyte proliferation and it may cause cytotoxicity in immune cell (lymphocyte) SCE...

Ngày tải lên: 20/06/2014, 00:20

... FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization Journal of Orthopaedic Surgery and Research 2011 6:8 Submit your next manuscript to BioMed Central and take ... osteoblast-like cells in normal media (NM) consisting of alpha MEM medium with 10% fetal calf serum (FCS), 1% antibiotic solution and 1% glutamine solution and subcultured the cells every to days The cells ... minutes and resuspending at a concentration of × 105 cells/ml For experiments, we grew the cells in the NM above, using multi-well plates After three days, the cells reach confluence and mineralization...

Ngày tải lên: 20/06/2014, 04:20

... FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization Journal of Orthopaedic Surgery and Research 2011 6:8 Submit your next manuscript to BioMed Central and take ... osteoblast-like cells in normal media (NM) consisting of alpha MEM medium with 10% fetal calf serum (FCS), 1% antibiotic solution and 1% glutamine solution and subcultured the cells every to days The cells ... minutes and resuspending at a concentration of × 105 cells/ml For experiments, we grew the cells in the NM above, using multi-well plates After three days, the cells reach confluence and mineralization...

Ngày tải lên: 20/06/2014, 07:20

Ngày tải lên: 07/08/2014, 15:20

... study; and manuscript preparation TM and HK contributed to MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; and analysis of T -cell proliferation ... plates and grown to confluence Osteogenesis and adipogenesis were induced as described previously [29] and [30], respectively) Anti-CD3-induced cell proliferation CD4 + T cells and accessory cells ... contributed to isolation and characterization of MSCs; MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; analysis of T -cell proliferation; design...

Ngày tải lên: 12/08/2014, 11:23

... transcriptional factors C/EBPα and C/EBPβ control cell differentiation and proliferation in multiple cell types and are suggested to be associated with eIF2~P (87, 88) Isoforms of C/EBPα and C/EBPβ can be ... her support, encouragement, and understanding iv ABSTRACT Lakshmi Reddy Palam REGULATION OF CHOP TRANSLATION IN RESPONE TO eIF2 PHOSPHORYLATION AND ITS ROLE IN CELL FATE In response to different ... and ε in mammals, and the yeast counterparts Gcn3p, Gcd7p, Gcd1p, Gcd2p, and Gcd6p, respectively (6, 21, 22) The γ and ε subunits facilitate the catalytic function of eIF2B, while the α, β, and...

Ngày tải lên: 24/08/2014, 12:32

... below 2.1.2.1.3 Cell lysis CHO cells were collected and re-suspended in suitable amount of SPHK buffer (described below) Cells were lysed by several cycles of freeze-thawing and total cell lysates ... U937 cells viability 5,000 and 10,000 cells were treated with 25μM of DMS or different compounds for 24 hours or 48 hours Cells were then stained with Trypan blue and counted under microscope Cells ... compounds and DMS were tested for their cytotoxicity in U937 cells U937 cell were incubated with 25μM of compounds or DMS for 24 hours and 48 hours in 96-well plates (5,000 or 10,000 cells/well)...

Ngày tải lên: 11/09/2015, 16:06

... suppressing SPHK activity in the stem cells, the balance between proliferation and differentiation could be lost, the cell growth was arrested, and the cells would then enter into certain differentiation ... PBS, and 200μl of distilled water was added into each well and at least five rounds of freeze-thaw (-80°C and 37°C) were performed to lyse the cells thoroughly Cells were then centrifuged and ... inhibit at all cell growth compared with untreated cells (DMSO=0) 3.2.3 Function of CP6 and DMS in Human BM- and AD- MSCs Differentiation Human BM- and AD- MSCs are multipotent cells that are...

Ngày tải lên: 11/09/2015, 16:06

... stem cells proliferation, what it functioned as: did it function as an extracellular mediator to bind with its receptors and promote cells proliferation? Or it penetrated into the cells and bind ... BM- and AD- MSCs proliferation Human BM- and AD- MSCs were cultured in different culture conditions and cells proliferation were tested by PicoGreen assay Results are the average + the standard ... the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally grouped and cultured...

Ngày tải lên: 11/09/2015, 16:06

... able to induce an excellent level of cell proliferation in serum-reduced media (1% FBS), especially in the human AD-MSCs, where 1μM S1P and 1% FBS could promote around 50% more cells growth (Figure ... accumulated on cell expansion and differentiation might also be applicable in other types of stem cells Also, it should be noticed that both human BMand AD- MSCs are primary cells, and thus they may possess ... differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem cell proliferation 157 ...

Ngày tải lên: 11/09/2015, 16:06

... intracellular sphingosine-1-phosphate and promotes cell growth and survival." J Cell Biol 147(3): 545-58 Olivera, A and S Spiegel (1993) "Sphingosine-1-phosphate as second messenger in cell proliferation ... sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells." Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the treatment of muscle and connective ... "Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue." Stem Cells 25(5): 1298-306 Shmelkov, S V., S Meeus,...

Ngày tải lên: 11/09/2015, 16:06

... one hand, it could act as an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and promote cell survival and cell growth On the other hand, ... messenger and as an extracellular ligand for specific cell- surface receptors to regulate cell survival, in either case S1P has been shown to promote cell proliferation, morphological changes and migration ... S1P in stem cell proliferation and multipotency maintenance It is well known that in stem cells, there is a balance between cell proliferation and differentiation, which directs the cells to proliferate...

Ngày tải lên: 11/09/2015, 16:06

... muticellular organisms arise from a single cell During this process, the cell number increases by cell division and the cells become different from each other by cell differentiation How cell ... 1999), including cell fate regulation (Macaluso et al., 2006), senescence (Funayama and Ishikawa, 2007), and apoptosis (Harbour and Dean, 2000b) Its dual function in cell cycle and cell differentiation ... RBR and MSI1, which are likely amongst the pivotal controls linking cell division and cell fate RBR and MSI1 participate in multiple protein complexes with a broad range of cellular functions and...

Ngày tải lên: 12/09/2015, 09:28

... and proliferation of colon cancer cells both in vitro and in vivo65 In addition, GSK3β inhibition reduces pancreatic cancer cell survival and proliferation6 6, and studies conducted in hepatocellular, ... role in cell death and cell fate We show that GSK3 regulates GPC maintenance and survival Depletion of GSK3 activity impairs proliferation, triggers apoptosis and reduces tumor stem cell frequency ... dissolved in 100% DMSO and used at the concentrations specified in our study 2.3 Cell Proliferation and Viability Assays To assess cellular proliferation rates, cells were harvested and plated into...

Ngày tải lên: 03/10/2015, 21:57