cell electropermeabilization and cellular uptake of small molecules the electrochemotherapy concept

... Molecular and Cellular Biology of Neuroprotection in the CNS Molecular and Cellular Biology of Neuroprotection in the CNS Edited by Christian Alzheimer Institute of Physiology University of Kiel ... processes that determine the fate of CNS neurons in the face of acute or chronic insults The book is written mostly from the viewpoint of the basic scientist who works at the cellular and molecular level, ... sparse and there is less evidence for the implicated modes of cell death Some studies have suggested the sudden release of excitatory amino acids from the cytoplasm into the extracellular space and...

Ngày tải lên: 08/04/2014, 12:51

... decrease in the amount of precipitation in the presence of high concentrations of Arg-HCl in the absence of the chaperone precluded analysis of the effect of this concentration on the protective ... aB-crystallin (i.e the effect of the additive on the aggregation of the target protein is independent and different from that of the chaperone) The standard errors associated with this theoretical percentage ... for days either in (A) the absence or (B) the presence of aB-crystallin (0.4 mgÆmL)1) and either 250 mM of Gly (d), 250 mM of Lys-HCl ()), 250 mM of Arg-HCl ( ) or 100 mM of GdnHCl (h) The buffer-only...

Ngày tải lên: 18/02/2014, 16:20

... in all cases and also in the lysates of the CHO-cells In the lysates of the LKB-Ez7 cells on the other hand partial splitting of the disulfide bond in the cell interior throughout the 30 incubation ... with cell penetrating peptides and the negligible role of structure forming properties of the peptide upon the entry of oligonucleotides into the cell interior (Figs and 7) Conjugation of the ... quantitative deductions on the basis of CLSM measurements alone The analogous parallel assessment of the cellular uptake by CLSM and GCE-LIF of the peptide conjugates with the 24-mer PTO, however,...

Ngày tải lên: 23/03/2014, 21:21

... that of the wild type, the GABA -uptake activities of the N-glycosylation 1628 Fig Determination of expression of GFP-tagged GAT1 mutants on the surface of transfected CHO cells and measurement of ... represents the mean ± SEM of three separate experiments The total protein of the cell surface and intracellular bands of each wild type or mutant were set at 100% (C) Measurement of GABA -uptake by ... DEYPRLLRNRRELFC(409–423) and SEDIVRPENGPEQPQAC (584–599), corresponding to sequences in the extracellular and intracellular loops of GAT1, were synthesized and used for immunization of rabbits Specificity of the antiserum...

Ngày tải lên: 19/02/2014, 17:20

... or off in different cells of the body Once inactivation occurs in a cell, all of the future generations of cells coming from that cell have the same chromosome inactivated in them as well (either ... cancer  ous cells, the nucleus may occupy most of the cell s volume Tumor cells also often lack the dif  ferentiated traits of the normal cell from which they arose Whereas normal secretory cells pro  ... disease of mole  cules and genes, and we even know many of the molecules and genes involved In fact, our increas  ing understanding of these genes is making possi  ble the development of exciting...

Ngày tải lên: 15/03/2014, 00:20

... rRNA bands (C) Effect of SCL RNAi on cell growth, compared with 29-13 and noninduced cells The numbers of 29-13 cells (diamonds), noninduced cells (triangles) and those induced by the addition of ... the TAP tag study This work was supported by the Grant Agency of the Czech Republic 204 ⁄ 09 ⁄ 1667, the Ministry of Education of the Czech Republic (LC07032 and 2B06129 and 6007665801) and the ... from cells resistant to lgÆmL)1 tetracycline and used for immunodetection of the HA3-tagged protein Northern and western blots Detection of Nfs-like mRNA isolated from the noninduced cells and cells...

Ngày tải lên: 22/03/2014, 21:20

... construction and use of docks and vessels associated with them, is at the discretion of the states or other levels of government entrusted with the management of public resources, and the legitimate ... conducted surveys of the opinions of residents of coastal counties in the state (2001) and of dock owners (2002) Some of their major findings include: • 75% of the residents of coastal counties ... owners and the general public felt that docks are harmful to the aquatic environment • 20% of the owners and 25% of the general public felt that docks detracted from the view of the waterbody and...

Ngày tải lên: 23/03/2014, 11:20

... [19], and therefore the presence of this substitution in the LEC14 epitope, must not be neglected The ensuing comparison between the properties of the LEC14 dodecasaccharide epitope and the core-fucosylated ... highlighted by these results, distinct preference of binding was determined for the B- and T-lymphoblastoid cells among the set of leukemia ⁄ lymphoma lines and to the mammary carcinoma cells among the ... glycoproteins presented on a cell surface by adsorbing the neoglycoprotein to the plastic surface of microtiter plate wells The homogeneity of the structure of the synthetic N-glycan, rigorously...

Ngày tải lên: 30/03/2014, 16:20

... of serial sections of the eyestalk revealed the presence of the FAMeT in the sinus gland, the X-organ neurosecretory cell clusters together with the lamina ganglionaries and the nerve fibers of ... point in the regulation of MF biosynthesis, the biochemical and biological properties of the gene encoding the FAMeT have not been characterized Thus, the study of the enzymes of the MF biosynthetic ... 3A) The neurosecretory cells and the laminar structure of the onion bodies of the X-organ [24] show the highest immunoreactivity to the anti-rFAMeT serum in the M ensis eyestalk (Fig 3B) The...

Ngày tải lên: 31/03/2014, 21:21

... immunofluorescence staining assay, participated in the discussion of results and drafted the manuscript GT participated in the design and coordination of the study and in the discussion of the ... were immunoblotted with the anti-NFYB and the anti-Flag antibodies for the detection of the endogenous NFYB and of CIITA, respectively The expression of the proteins in whole cell extracts was also ... in the presence of CIITA were performed by immunofluorescence and confocal microscopy In the absence of CIITA, Tax-2 localized both in the cytoplasm and the nucleus in the great majority of the...

Ngày tải lên: 18/06/2014, 22:20

... clinical disease Taken together, the results of the present study and from previous ones on the role of CCR1 and its ligand CCL3 in the pathogenesis of MS [39,40,42] and EAE [22,44,46], have provided ... crucial for the understanding of therapeutic modulation and the validation of CCR1, CCR2 and CCR5 as feasible targets for therapeutic intervention in MS Background Multiple sclerosis (MS) is the most ... from Finland, suggesting that the lack of CCR5 does not protect from MS, but rather it may predispose to the chronic course of the disease [69] This would further imply that in view of the redundancy...

Ngày tải lên: 19/06/2014, 22:20

... immunofluorescence staining assay, participated in the discussion of results and drafted the manuscript GT participated in the design and coordination of the study and in the discussion of the ... were immunoblotted with the anti-NFYB and the anti-Flag antibodies for the detection of the endogenous NFYB and of CIITA, respectively The expression of the proteins in whole cell extracts was also ... in the presence of CIITA were performed by immunofluorescence and confocal microscopy In the absence of CIITA, Tax-2 localized both in the cytoplasm and the nucleus in the great majority of the...

Ngày tải lên: 20/06/2014, 04:20

... reported that the higher energy absorption of the lower relative density foams is a result of the larger deformation, the bending and buckling of the cell walls and edges.3 The differences in the absorbed ... direction Then, the micrographs were analysed using Image Pro Plus Software to determine the average size of the foam cells The average cell sizes of the samples were determined from measurements of ... indicates the measurement of the stiffness of the unvulcanised rubber at the lowest point of the cure curve.7 The results indicate that the blowing agent concentration did not affect the compound...

Ngày tải lên: 07/08/2014, 14:20

... tnedneped-ygrene ,evitceffe rof wolla yeht esuaceb stnega noitcefsnart sa desu eb nac sPNM eht taht tseggus ylgnorts seiduts ruO hcraeser lacigoloib rof lacitirc si ,erofereht ,noitcefsnart AND tneiciffE ekatpu ... ralullec wolla ot AND fo egrahc eht sksam dna tnemnorivne ralullecartxe eht ni AND eht stcetorp hcihw ,yteiom cinoitac a yb desnednoc si AND ,tnegaer noitcefsnart eht fo sseldrageR AND htiw dexelpmoc ... Co4 ta nim 03 lanoitidda na rof detabucni erew Co4 ta detabucnierp sllec( nim 03 lanoitidda na rof s)CTIR(2OiS@PNM htiw detaert neht erew dna ,ylevitcepser ,nim 01 rof ]2[ A nicymolifab Mµ 50.0...

Ngày tải lên: 07/08/2014, 18:21

... stluseR )C2 giF( snoitces eseht ni dnuof saw gnillebal cificeps on dna ,snoitces tset wef a rof dettimo saw ydobitna yramirp eht ,lortnoc a sA detnuom gnieb erofeb nilyxotameh htiw deniatsretnuoc ... noitagufirtnec yb detcelloc saw xelpmoc enummi ehT h rof esorahpeS-A nietorp fo yrruls %05 eht fo lµ 03 dna ydobitna DLP-itna eht htiw detabucni erew setasyl llec deraelcerP nim 03 rof esorahpeS-A ... yrtsimehcotsihonummi rof desu dna ,nisoe dna nilyxotameh htiw deniats erew snoitces niffaraP niffarap ni deddebme dna reffub etahpsohp ni edyhedlamrofarap %4 ni dexif saw sitset etisoppo ehT sisylana nietorp rof...

Ngày tải lên: 07/08/2014, 20:23

... identify the proportions of CD18+ and CD62L+ cells The discrimination between positive and negative cells was set using the isotype control The mean fluorescent intensity (MFI) of each cell in ... housing systems, feeding, climate and management Therefore, the aim of this study was to evaluate leukocyte numbers and the expression of the adhesion molecules CD62L and CD18 on blood neutrophils, ... affect the adhesion molecules of lymphocytes and therefore they can migrate into the tissues Our results have shown a marked decline at calving in serum concentrations of vitamins A and E, and in...

Ngày tải lên: 12/08/2014, 15:20

... favorable outcome of an assignment when they know how their effort can affect or bring to the general success of the company As long as they understand the importance of the task, they can adjust ... basis of the meaningfulness of their jobs 2.2 Theoretical and conceptual framework 24 “Adams (1965) equity theory as presented” in Spector (2008) and Herzberg’s “two-factor theory provided theoretical ... fair and equitable return for their contributions to their jobs Second, the theory assumes that people tend to compare what they perceive to be the exchange they have with their employers” The other...

Ngày tải lên: 23/08/2014, 01:57

... calculate the Z’ factor using the following formula: Z’ factor Where δp= standard deviation of the control reaction, δn= standard deviation of the inhibition reaction, µp= average of the points in the ... (Cys243) of ALDH3A1 and inhibits it activity (Khanna et al., 2011) The assay was carried out for 15 minutes and then the rates of the control and inhibition reactions (384 points for each set of reactions) ... concentration) was added to induce the synthesis of ALDH3A1, and the cells were incubated for an additional 16 hours at 16°C, and collected by centrifugation The cell pellets were resuspended in...

Ngày tải lên: 24/08/2014, 09:51

Ngày tải lên: 29/10/2014, 22:15

... the other hand, DMSO was the only chemical which reduced larval activity in the dark and was among the two substances (the other was SB) that exhibited the same effect in the light phase The other ... infants [12], and the association between the antibiotic chloramphenicol and the so-called “gray baby syndrome” [7] On the other hand, there are concerns over hazardous effects of some PPCP substances ... triplicate, consisting of 0.2 L of synthesised cDNA, 0.53 M of each primer and 7.5 µl reaction mix (containing reaction buffer and thermostable polymerase) in a total volume of 15 L The thermal cycle...

Ngày tải lên: 15/05/2015, 00:37