cell cycle regulated transcription in yeast

... the cell, working with other factors to halt the cell cycle and mediate DNA repair. Interestingly, a much higher proportion of NFT were labeled in AD cases than in control cases. These findings ... neuronal cell cycle are un- clear. In this regard, given that a dysregulated cell cycle is a characteristic of cancer, we speculated that altera- tions in oncogenic proteins may play a role in neurodegeneration. ... in all cases of Alzheimer disease. These findings not only further define the cell cycle reentry phenotype in Alzheimer disease but also indicate that the neurofibrillary tangles which define...

Ngày tải lên: 26/10/2012, 10:04

Ngày tải lên: 22/06/2014, 19:20

... gene transcription [3]. Interest in control mechanisms of cyclin B1 and cyclin B2 cell cycle- dependent transcription began early [3,30–33]. When investigating the regulation of human cyclin B1 transcription, ... alterations during the cell cycle, making it unlikely that protein binding to the CDE ⁄ CHR is affected by change in DNA methylation during different phases of cell division [34]. Another cell cycle gene, ... Promoter binding factors regulating cyclin B transcription in the sea urchin embryo. DNA Cell Biol 14, 869–881. 34 Tscho ¨ p K & Engeland K (2007) Cell cycle- dependent transcription of cyclin B2...

Ngày tải lên: 16/02/2014, 09:20

... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays. In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ... 2A,C; in C, only a faint signal was detected). The above results indicated that the C-terminus of Rio1p might play a role in the interaction with Cka2p in yeast cells. To determine domains of ... G 1 phase [11]. Keywords atypical protein kinase; casein kinase 2 substrate; cell- cycle phase-dependent degradation; protein–protein interactions; Rio1 protein kinase 2 1 Correspondence M. Angermayr E-mail:...

Ngày tải lên: 18/02/2014, 16:20

... of the geminin protein differs between cell line s, r esulting in gross differences in relative amounts of Cdt1 and its i nhibitor in different cell lines. I t would b e interesting to investigate ... during the cell cycle in human cells, accumulating only du ring the G1 ph ase, when licensing is legitimate [13]. When cells exit the cell cycle, licensing in lost, a nd is est ablished again ... full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti-Cdt1 and anti-geminin Ig. See Materials...

Ngày tải lên: 07/03/2014, 16:20

... proteins act as mediators of various cell cycle regulators, in- cluding Cdk activity. The CKS (Cdc28 kinase subunit) protein family is the mammalian homolog of the yeast Cdk binding proteins Cks1 ... Chaperone protein involved in protein folding, Dix et al. 1996 70 kDa protein 2 (HSP70-2) leads to spermatogenic arrest at metaphase stage in knockouts. Cell Cycle Regulation in Mammalian Germ Cells 351 Table ... However, understanding cell cycle regulation in mammalian germ cells has considerably lagged behind that of the mitotic cell cycle. Challenges to investigations of meiosis in mammals have included the...

Ngày tải lên: 25/10/2013, 21:20

... variant containing the DNA binding motif but not the pRb-binding or transactivation domain or introduction of a competitor plasmid containing multiple E2F binding sites, preventing the binding of E2F ... The pocket-domain is responsible for binding to proteins containing LXCXE motifs while the pocket-domain and the C-domain are involved in binding E2F proteins. Mdm2 (as well as c-Abl) binds to the C-domain. ... overlapping roles in controlling cell cycle exit upon growth-inhibiting signals. Only ablation of all pocket proteins fully alleviated a cell cycle arrest upon serum withdrawal, cell- cell contact inhi- bition,...

Ngày tải lên: 25/10/2013, 21:20

... for linking cell cycle regulation to internal and external signaling pathways via regulated protein kinase activities. 1 Introduction The importance of ubiquitin-mediated proteolysis for cell cycle ... remain to be elucidated (Kominami et al. 1998; Suzuki et al. 2000). Interestingly, there are three classes of related F- box proteins in both yeast and mammalian cells that are involved in cell cycle control. ... the spindle assembly checkpoint, which will be discussed in greater detail below, maintains Cdc20 in an inactive state until bipolar attachment of The ubiquitin-proteasome pathway in cell cycle...

Ngày tải lên: 25/10/2013, 21:20

... HeLa cell cycle in a site-specific manner. Cyclin-dependent protein kinase is involved in the phosphorylation of MCM4 To determine which kinase is involved in the phos- phorylation of MCM4 in phases ... of DNA in a single cell cycle. Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7]. Targets of the kinase in the regulation of DNA replication include ... proteins on licensed chromatin in the G 1 phase was detached from chromatin. Phosphorylation of MCM4 and other fac- tors may be involved in stronger binding of MCM4 protein to chromatin in the...

Ngày tải lên: 16/03/2014, 13:20

... protein in CHO-BP1a cells, p21 and p27 proteins were markedly induced, as shown by immunoblot analysis (Fig. 4B). In accordance with the induction of these cdk inhibi- tors, SREBP-1a-expressing cells ... activation of a cyclin E–cdk2 complex during the G 1 phase of the human cell cycle. Science 257, 1689–1694. 20 Weinberg RA (1995) The retinoblastoma protein and cell cycle control. Cell 81, 323–330. 21 ... (1998) Characterization of two Chinese hamster ovary cell lines expressing the COOH-terminal domains of sterol regulatory element-binding protein (SREBP)-1. Cell Struct Funct 23 , 187–192. 33...

Ngày tải lên: 23/03/2014, 07:20

... colony-forming cells was determined as indicated in the methods section. Fig. 6. Effect of fatty acid-unsaturation-inducer dioctylphtalate on h-Bax-induced cell death kinetics. WtB1cellsweregrowninacom- mercial ... measured in Yeast Nitrogen Base medium (7.05 ± 0.05; n ¼ 3). Kinetics of Bax-induced cell death varied accordingly to the extent of Bax-induced mitochondrial lipid oxidation. In the minimal medium without ... dioctylphtalate, Bax-induced cell death kinetics was much slower than in Yeast Nitrogen Base medium (Fig. 6). The addition of dioctylphtalate restored cell death kinetics comparable to experiments in Yeast...

Ngày tải lên: 23/03/2014, 21:20

... trends in the Stanford yeast cell- cycle data to uncover genome- wide oscillations in expression in  4500 of the 6178 gene expression profiles. These were typically of cell- cycle or half -cell- cycle ...  40-min cycles (Fig. 3A,C) are similar to one another in forming a bowl or conical shape around the steady state. In other projections of the phenelzine-treated cells, the increase in cycle ... the epithelial cells of the gastro-intestinal tract are similar – if on different time scales. In the gastrointestinal tract of mammals the cell- cycle time of a particular cell is in the range 5–10...

Ngày tải lên: 30/03/2014, 04:20

... colony as a whole. An intriguing candidate is the lectin-like protein involved in flocculation, a cell wall protein that binds to mannose chains on the surface of other cells to provide the physical ... cultured yeast cells in terms of transcriptional activity led to the notation that collective modes of whole population of cells indeed in uence transcriptional machinery. This synchronization, having a ... unity indicates the non-noisy character of the extracted modes, pointing to the highly coordinated gene expression behaviour of these cells. HeLa cells display whole genome cell cycle independent...

Ngày tải lên: 30/03/2014, 08:20

... cell cycle [11–15]. Cer- tain transacting proteins such as HuR were proposed as regulators of changes in mRNA stability during the cell cycle [15]. In this context it is of interest that during vertebrate ... elutriated cells. The cell cycle distribution of the cell fractions is shown in Fig. 4. AB CD Fig. 1. Steady-state levels of cyclin mRNAs in enriched cell cycle fractions of mouse EL4 cells. Cells ... an increase in late S and G 2 ⁄ M phase confirming previous observations [26,27], but no cell cycle- dependent change in stability. The cyclin E1 mRNA was increased in the G 1 phase of the cell cycle, ...

Ngày tải lên: 30/03/2014, 20:20

Ngày tải lên: 31/03/2014, 01:20

Ngày tải lên: 31/03/2014, 21:21

Ngày tải lên: 08/04/2014, 12:50