cdna and real time quantitative rt pcr
Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... continued interest and support We would also like to acknowledge the excellent technical support of C Saunders who performed the PBMC assays This work was partially supported by RR13154 and RR00166 ... within macaque RV1 rhadinoviruses In this paper, we report the development of a sensitive and specific TaqMan QPCR assay and its use in detecting and quantitating RV2 rhadinoviruses from different ... RV1 and RV2 rhadinovirus species from Macaca nemestrina, RFHVMn and MneRV2 The ORF 59 and ORF 60 genes show high levels of homology between the related rhadinoviruses, KSHV and RRV, with 52% and...
Ngày tải lên: 18/06/2014, 22:20
báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... continued interest and support We would also like to acknowledge the excellent technical support of C Saunders who performed the PBMC assays This work was partially supported by RR13154 and RR00166 ... within macaque RV1 rhadinoviruses In this paper, we report the development of a sensitive and specific TaqMan QPCR assay and its use in detecting and quantitating RV2 rhadinoviruses from different ... RV1 and RV2 rhadinovirus species from Macaca nemestrina, RFHVMn and MneRV2 The ORF 59 and ORF 60 genes show high levels of homology between the related rhadinoviruses, KSHV and RRV, with 52% and...
Ngày tải lên: 20/06/2014, 04:20
Báo cáo y học: "Human rheumatoid arthritis tissue production of IL-17A drives matrix and cartilage degradation: synergy with tumour necrosis factor-α, Oncostatin M and response to biologic therapies" ppt
... participated in study design and data analysis JMcC, MC and OS performed some experiments BB and OF participated in study design and data analysis UF and DV conceived the study and developed the study ... angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures Arthritis Rheum 2006, 54:3152-3162 Page 11 of 12 (page number not for citation purposes) Arthritis ... were pooled from each time point and stored at -80°C for analysis of proMMP-1, proMMP-13, TIMP-1, MMP-2 and MMP-9 Cartilage and RASFC experiments were performed in duplicate Cartilage explants were...
Ngày tải lên: 09/08/2014, 14:22
Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death
... support and love throughout my life Special thanks belong to my wife and two sons The knowledge of them being there has been of great encouragement and importance for me Without their support, ... shown to be linked to the intracellular levels of O2.- and H2O2 (Pervaiz S and Clement MV, 2002 and 2004; Clement MV and Pervaiz S, 1999 and 2001) Interestingly, an increase in intracellular O2.- ... HCO3dependent transporters (Cl HCO3- exchanger, Na+- HCO3- cotransporter) Na+-driven Cl HCO3- exchanger couples an influx of Na+ and HCO3- to an efflux of Cl- and H+ (so that NaHCO3 comes in and HCl goes...
Ngày tải lên: 16/09/2015, 08:31
Báo cáo y học: "Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline"
... forty reports of the successful in vitro uptake of exogene constructs (transgenes) by animal sperm cells [33, 34] A majority of these reports provide evidence of post-fertilisation transfer and ... non-prohibitive cost and expertise requirements; (c) minimal risk of causing insertional DNA damage; (d) low rate of mosaicism; (e) high DNA carrying capacity; (f) the ability to permit adequate and controlled ... implicates the particular RVVs employed in this trial [27, 28, 29] Indeed, clinical trials involving this particular RVV-based therapy have been halted pending further investigations and pre-clinical...
Ngày tải lên: 03/11/2012, 10:01
DEVELOPING, USING AND ANALYZING THE RESULTS OF INTERVIEWS TO DETERMINE THE FEELINGS OF FOREIGN TOURISTS ON THEIR VISIT TO VIET NAM
... countries at this time Therefore, they spend more time and money for travelling, and for relaxing in some Southern Eastern Asian countries: Thailand, Singapore, Laos, Cambodia, and Vietnam And the reason ... a Chart of Nationality This pie chart shows us the Nationality of the tourist coming to Vietnam at this time, and divided into three occupations: tourists from European, Oceanian and North American ... designing, asking questions and analyzing their results in a short report? So that, it is very interesting with the student because it helps student to developing higher skills and abilities, which...
Ngày tải lên: 25/04/2013, 15:42
A New Technique Using Headspace Gas Monitoring to Determine Carbon Source Addition in a BNR Process
... supported by grants form the National Science and Engineering Research Council (NSERC) of Canada and the Dayton and Knight Ltd., West Vancouver, BC, Canada The excellent technical support provided ... inside the reactor and the carrier gas flow rate were probably the key factors resulting in this delay However, according to ORP and pH profiles, this time lag seemed to be consistent and it did not ... 120 140 160 180 200 Time elapsed (min.) 7.30 100 120 140 160 180 200 Time elapsed (m in.) Figure CO2 and pH profiles of NaAc addition Table 1: Acetate concentration estimation and analytical verification...
Ngày tải lên: 05/09/2013, 08:40
Use of an extension of the park's transformation to determine control laws applied to a non sinusoidal permanent magnet synchronous motor
... take into account the coupling of d and q phases Figure 5b: "abc" frame vector control: torque and currents versus time for a controller wth slow response time (1 ms) Torque I T ref t t ib ia ... 5c: "abc" frame vector control: torque and currents versus time for a controller with fast response time (0.5 ms) With the controller with slow response time the currents are not able to follow ... ib and i, and the ideal currents i, rd, nf and i, nf (figure 6b) ib voltage during the s W y state The "dq" frame is better than " a h " frame It need more complex implementation, but the band...
Ngày tải lên: 03/01/2014, 19:50
Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... misfold and induce a cellular unfolded protein response We propose that glycosylation is important for the folding of HFE and is essential for transport and exit of the protein from the ER The importance ... anti-HA and anti-BiP Ig, and processed for fluorescence microscopy Co-localization masks were created as described in Materials and methods, and represent areas with overlapping green and red ... determined and although the two proteins interact over a relatively large interface, it has been shown that residues V100 and W103 (V78 and W81 of the mature protein) are of particular importance...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: The transcription factor ZBP-89 suppresses p16 expression through a histone modification mechanism to affect cell senescence doc
... isolated and reverse transcribed, and p16 mRNA was measured by real- time PCR b-Actin was used as an internal control **P < 0.01, *P < 0.05 (n = 3) (E, F) HDAC3 and HDAC4 participated in p16 regulation ... extraction and real- time quantitative PCR Total cellular RNA was extracted from the 293T cells according to the Promega Total RNA Isolation System manual RNA was resuspended in RNase-free water and ... by HDAC3 and HDAC4 overexpression in a dose-dependent manner (Fig 3B,C) The endogenous p16 mRNA level was also decreased upon HDAC3 and HDAC4 overexpression, as revealed by real- time PCR (Fig...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Differing molecular mechanisms appear to underlie early toxicity of prefibrillar HypF-N aggregates to different cell types potx
... presence of the redox fluorescent probe and measuring the fluorescence of DCFH-DA The data are reported as a proportion of the values determined at time and are expressed as means ± SD of four ... mitochondrial status, cytochrome c and AIF release, Bcl-2 expression, and caspase and caspase activation support different scenarios in the response of Hend and IMR90 cells to the same toxic aggregates ... Hend and IMR90 cells exposed to HypF-N aggregates, plated on glass coverslips and fixed at various exposure times (Fig 3) Indeed, in Hend cells we found an increase in free Ca2+ that was earlier and...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx
... cells were washed and then further incubated with nM tPA and lM TAPI-1 at 37 °C for h The incubated cells were collected, washed and dissolved in the SDS/PAGE sample buffer and subjected to immunoblotting ... culture was washed three times with NaCl/Pi containing mm CaCl2, mm MgCl2 and mm glucose, incubated with nm tPA and lm TAPI-1 as an MMP inhibitor at 37 °C for h, and washed three times The cells were ... and at 460 nm for emission, using a Plate Chameleon spectrofluorometer (Hidex, Turku, Finland) Acknowledgements We thank K Moriyama and A Kurosawa for technical support of protein sequencing and...
Ngày tải lên: 17/03/2014, 17:20
Báo cáo khoa học: Wild-type p53 enhances annexin IV gene expression in ovarian clear cell adenocarcinoma docx
... and RNA were extracted from two CCA (OVTOKO and OVISE) cells lines and one non-CCA (MCAS) cell line, and then ANX4 protein (A) and mRNA (B) levels were compared by western blotting and real- time ... mRNA and protein levels of ANX4 and p21 were measured by western blotting and real- time RT- PCR analyses The p53 protein levels were also assessed by western blotting to verify that MMC and nutlin-3 ... CCA than non-CCA cell lines and tissues [13] We confirmed this finding by western blotting and real- time RT- PCR analyses using cell lines originating from CCA, OVTOKO and OVISE cultured cell lines,...
Ngày tải lên: 22/03/2014, 16:20
Cancer Research UK’s strategy 2009–2014: Cancer Research UK’s aim is to reduce the number of deaths from cancer. Our future plans are ambitious, but they are in line with the challenge and the responsibility we face. docx
... opportunities via in-house development It will consolidate its operations in the US and Australia and clarify the proportion of time dedicated to third party business (developing cancer opportunities ... tests available today and potential new advances in treatment Understanding cancer We have made many significant breakthroughs in understanding how cancer starts and develops and helped characterise ... Maintain and, where possible, strengthen a broad and balanced portfolio of world-class research in the UK, directed at understanding the biology and causes of cancer - Maintain a strong portfolio...
Ngày tải lên: 22/03/2014, 16:21
Báo cáo khoa học: Transcription factor specificity protein 1 (SP1) and activating protein 2a (AP-2a) regulate expression of human KCTD10 gene by binding to proximal region of promoter pot
... mRNA and protein levels using quantitative real- time RT- PCR and western blot through overexpressing or suppressing AP-2a or Sp1 As shown in Fig 7A, mRNA levels in HeLa cells were measured using quantitative ... (Invitrogen); RT- PCR was carried out using a twostep strategy following the manufacturer’s manual (Promega) The primers for quantitative real- time RT- PCR of KCTD10 were shown in Table 1, and b-actin ... human KCTD10 after overexpressing, or suppressing Sp1 and AP-2a (A) mRNA levels in HeLa cells were measured using quantitative real- time RT- PCR, and presented as base 10 logarithms A1, mRNA levels...
Ngày tải lên: 30/03/2014, 02:20
Báo cáo khoa học: Leishmania donovani bisubunit topoisomerase I gene fusion leads to an active enzyme with conserved type IB enzyme function doc
... Frohlich RF, Andersen FF, Westergaard O, Andersen HA & Knudsen BR (2004) Regions within the N-terminal domain of human topoisomerase I exert important functions during strand rotation and DNA binding ... opening and closing of the protein clamp during binding and release of DNA [11,12] Champoux and his group have previously reported their findings on human topoisomerase I that has been artificially ... some biochemical properties and preferential sensitivities to CPT [14,15] Thus, the key questions arise of what will happen to the enzyme architecture and catalytic property if the two subunits...
Ngày tải lên: 30/03/2014, 10:20
introduction to p-adic numbers and p-adic analysis - a. baker
... many important topics such as differentiability, integration and so on For these I suggest you look at Koblitz [3] I particularly recommend his discussion of the Γ-function and integration and the ... {units} is the set of units in Z/n and {zero divisors} the set of zero divisors Furthermore, (a) z is a zero divisor if and only if gcd(z, n) > 1; (b) u is a unit if and only if gcd(u, n) = Proof ... following properties: (a) ordp x = ∞ if and only if x = 0; (b) ordp (xy) = ordp x + ordp y; (c) ordp (x + y) min{ordp x, ordp y} with equality if ordp x = ordp y Proof (a) and (b) are easy and left...
Ngày tải lên: 31/03/2014, 15:19
