cd123 nuclear relb dcs are located in perivascular regions of ra synovial tissue
Báo cáo y học: "Action of fibroblast growth factor-2 on the intervertebral disc" docx
Ngày tải lên :
09/08/2014, 10:23
... demonstrated the presence of FGF2 in human herniated IVD tissue [18,19] and in injured AF tissue in adult merinos [20] While these findings demonstrate the localization and/or expression of FGF2 in ... cell proliferation in bovine spine discs [52], it failed to increase ECM synthesis in parallel in our study, resulting in clustering of cells with little surrounding ECM – a hallmark of arthritic ... localization of an intracellular binding protein related to the fibroblast growth factor (FGF) receptor is temporally associated with the nuclear trafficking of FGF-2 in proliferating epiphyseal...
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Báo cáo Y học: The mechanism of nitrogen monoxide (NO)-mediated iron mobilization from cells NO intercepts iron before incorporation into ferritin and indirectly mobilizes iron from ferritin in a glutathione-dependent manner pot
Ngày tải lên :
08/03/2014, 22:20
... performed Examining the intracellular distribution of 59Fe in control cells, ferritin-59Fe increased as a function of reincubation time (Fig 3B) In fact, after a reincubation time of 240 in control ... incorporation into ferritin gradually increasing as a function of reincubation time (Fig 3B) In contrast, in cells treated with GSNO, ferritin-59Fe levels decreased after 90 of reincubation In fact, ... mechanism(s) involved in the intracellular trafficking and delivery of Fe to ferritin remain unclear, although intermediates of low Mr [46] or high Mr (e.g metal-binding chaperones) [47] could be involved...
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Báo cáo Y học: O-GalNAc incorporation into a cluster acceptor site of three consecutive threonines Distinct specificity of GalNAc-transferase isoforms pot
Ngày tải lên :
17/03/2014, 10:20
... GalNAc incorporation was Thr-2 resulting in the formation of FITC-PT*TT*°PLK in the case of the action of pp-GalNAc-T1 and T2 according to the protein sequencing analysis (Fig 6) Effects of modification ... Clausen, H & Irimura, T (1999) Incorporation of N-acetylgalactosamine into consecutive threonine residues in MUC2 tandem repeat by recombinant human N-acetyl-D-galactosamine transferase-T1, T2 and ... GRAMS/386 software Amino acid sequencing Pulsed liquid Edman degradation amino acid sequencing of glycopeptides was performed with the Applied Biosystems 490 Procise protein sequencing system (Perkin...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
Ngày tải lên :
23/03/2014, 07:20
... potential of these compounds by examining their scavenging effects on O2– from PMA-stimulated PMNs, with or without inhibition of NADPH oxidase as an index of translocation of p47phox The generation of ... were incubated with 100 lm each compound in 100 lL of RPMI-1640 medium containing 2% fetal bovine serum at 37 °C, in an atmosphere of 95% air and 5% CO2 After 24 h of incubation, we added 10 lL of ... reduction of H2O2, and a variety of organic hydroperoxides, resulting in effective elimination of various ROS in vivo and in vitro [23] The selenium molecule in GPX plays a crucial role in the metabolism...
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Báo cáo khoa học: The fate of newly synthesized V-ATPase accessory subunit Ac45 in the secretory pathway doc
Ngày tải lên :
31/03/2014, 09:20
... from adopting its normal conformation or from interacting with its binding partner Tunicamycin inhibits N-linked glycosylation and cleavage of intact Ac45 As N-linked glycosylation of intact Ac45 ... Leupeptin is a thiol protease inhibitor that inhibits degradation of proteins in lysosomes [43] The weak base chloroquine and the V-ATPase-specific inhibitor bafilomycin A1 (Baf) are known to inhibit ... shown), indicating that this product represents the N-terminal fragment of Ac45 The drugs used did not significantly change the amount of the 42–44-kDa Fig Effect of inhibitors of intracellular transport...
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Báo cáo y học: " Human immunodeficiency virus type 1 Vpr: oligomerization is an essential feature for its incorporation into virus particles" ppsx
Ngày tải lên :
12/08/2014, 04:20
... that Vpr mutants that are defective in virion incorporation are defective in Gag interaction and virion incorporation Figure Role of Vpr oligomerization in incorporation of Vpr into virus particles ... targeting single residues in Vpr indicated that amino acids in the N terminal region including the helical domains are essential for stability and virion incorporation and a region comprising the ... helical domain III (isoleucine-leucine residues) in Vpr are required for interaction with Gag, thus virion incorporation [26,39,48] Our results on Vpr-Gag interaction using BiFC (Table 2) are in agreement...
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Báo cáo y học: " The cellular source for APOBEC3G’s incorporation into HIV" pdf
Ngày tải lên :
13/08/2014, 01:20
... blot In these gradients, hA3G in the S1 was found in both LMM fractions (including fractions and 4) and HMM fractions (including fractions and 8), as shown in the top panel of Figure 1B hA3G in ... amount of radio-labeled hA3G in each fraction was determined by autoradiography and presented graphically in Figure 3B The total amount of hA3G in each fraction was set as 100% Results show that radio-labeled ... ability of hA3G to bind to Gag is insufficient for its incorporation into HIV-1 Several amino acid residues (i.e., W127) within the N-terminal part of the linker region play an important role in mediating...
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Báo cáo y học: "Tetherin does not significantly restrict dendritic cell-mediated HIV-1 transmission and its expression is upregulated by newly synthesized HIV-1 Nef" ppsx
Ngày tải lên :
13/08/2014, 01:20
... Nef-dependent induction of tetherin in DCs in addition to expressing Vpu as an antagonist of tetherin Given the apparent transient nature of the Nef-induced tetherin expression in DCs, it is possible ... cytokines act in an autocrine manner to induce transient tetherin expression in DCs as part of an innate immune response to HIV-1 infection Moreover, an increase in cellular content of tetherin ... replication in mature DCs Intriguingly, we found that tetherin was induced by HIV-1 infection of iDCs in a Nef-dependent manner, suggesting that tetherin upregulation is an innate immune response of DCs...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
Ngày tải lên :
21/02/2014, 03:20
... chlorate, in inducing structural alteration of HS chains, prevents the bFGF binding Our results indicate that the presence of sodium chlorate abolishes the bFGF biological effects in developing ... cells were incubated for 24 h with testosterone substrate (200 ngáml)1) in the presence or not (control) of FSH (100 ngáml)1) and of bFGF increasing concentrations Determination of estradiol production ... (control) in the presence of mM dbcAMP or 10 lgámL)1 cholera toxin and/or ngámL)1 of bFGF Estradiol production was determined by radioimmunoassay Values are expressed in pg per 106 cells and are representative...
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Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt
Ngày tải lên :
16/03/2014, 16:20
... (visualized with iodine vapour) were scraped into vials for determination of the radioactivity by liquid scintillation counting using Lumagelsafe scintillant (Perkin Elmer Life Sciences) Preliminary experiments ... [3H]oleate incorporation into lipids was linear over a period of h under the conditions used Incorporation of [3H]glycerol into triacylglycerol was determined using a modification of the method ... lipoproteins which pass into the blood via the thoracic duct and are then rapidly metabolized by lipoprotein lipase in extrahepatic capillary beds, removing some of the triacylglycerol and leaving...
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Báo cáo khoa học: Translational incorporation of L-3,4-dihydroxyphenylalanine into proteins docx
Ngày tải lên :
16/03/2014, 22:20
... Mobilities of marker proteins (sizes in kDa; prestained in B) were as indicated 3164 Fig Proteins are synthesized in good yields in vitro using DOPA in place of tyrosine Duplicate in vitro synthesis ... during the reaction DOPA is incorporated into proteins during cell-free synthesis To show that DOPA was incorporated into the translated proteins, we first used a redox staining method employing ... of substitution of DOPA for tyrosine was investigated in a preparative in vitro protein synthesis FEBS Journal 272 (2005) 3162–3171 ª 2005 FEBS Translational incorporation of DOPA into proteins...
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Báo cáo khoa học: Calcium-induced activation and truncation of promatrix metalloproteinase-9 linked to the core protein of chondroitin sulfate proteoglycans pot
Ngày tải lên :
17/03/2014, 10:20
... hemopexin-like domain (C domain) of human gelatinase A (matrix metalloproteinase-2) requires Ca2+ for fibronectin and heparin binding: binding properties of recombinant gelatinase AC domain to extracellular ... shortening of the C-terminal domain It appeared that the core protein of the CSPG was cleaved prior to the processing and shortening of the C-terminal hemopexin-like domain, as the 100 kDa form appeared ... separating gel Fig Urea inhibits both the CaCl2-induced release of low Mr forms of the gelatinase and cABC degradation of the CS-chains in the complex Four micrograms of purified CSPG was incubated...
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Báo cáo Y học: Incorporation of 3-nitrotyrosine into the C-terminus of a-tubulin is reversible and not detrimental to dividing cells potx
Ngày tải lên :
17/03/2014, 10:20
... Rate of increase of Glutubulin during in vitro incubation was the same in both cases (data not shown), indicating that replacement of tyrosine by 3-nitrotyrosine at the C-terminus of a-tubulin ... behavior of nitrotyrosinated tubulin (and of tyrosinated tubulin for comparison) during reconstitution of microtubules from soluble rat brain extract For monitoring the tubulin isospecies, we used radioactive ... parameters using F12 medium We found that tubulin Ó FEBS 2002 Incorporation of nitrotyrosine into a-tubulin (Eur J Biochem 269) 5043 Fig Reversible incorporation of nitrotyrosine into tubulin...
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Báo cáo khoa học: Dissection of LolB function – lipoprotein binding, membrane targeting and incorporation of lipoproteins into lipid bilayers potx
Ngày tải lên :
23/03/2014, 05:22
... indicated times in the presence of the indicated concentrations of arabinose by inoculation of portions of the cultures into fresh medium (B) The same amounts of cells grown in the presence of ... present in the outer membrane and be involved in the membrane incorporation of lipoproteins However, as mLolB was found to be able to catalyse the incorporation of lipoproteins, even into liposomes, ... mLolB-dependent membrane incorporation of lipoproteins (A) The incorporation of Pal into liposomes was examined at 30 °C for the specified times, as in Fig 5B, with spheroplast supernatants containing the...
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Báo cáo khoa học: Incorporation of ZP1 into perivitelline membrane after in vivo treatment with exogenous ZP1 in Japanese quail (Coturnix japonica) doc
Ngày tải lên :
23/03/2014, 07:20
... successfully incorporate into the PL Results Incorporation of exogenously injected ZP1 into quail PL To determine whether ZP1 injected intravenously into a bird was incorporated into the PL of the ... that the C-terminal half of the ZP domain of ZP1 contains a binding site for ZP3 [19] Similarly, Okumura et al also demonstrated that ZP1 in the serum of laying hens specifically interacts with ZP3 ... purified from the PL of the largest follicles was injected intravenously into birds, it did not become incorporated into the PL This was not a result of denaturation of the protein during the purification...
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Báo cáo sinh học: " Genetic incorporation of the protein transduction domain of Tat into Ad5 fiber enhances gene transfer efficacy" docx
Ngày tải lên :
18/06/2014, 18:20
... higher cell-binding activity in almost all of the cells we examined, including both highCAR and low-CAR containing cells Shown in Fig are results obtained in two representative cell lines: high-CAR ... interactions of HIV-I Tat protein with size-defined heparin oligosaccharides J Biol Chem 1999, 274(40):28198-28205 Spillmann D, Witt D, Lindahl U: Defining the interleukin-8-binding domain of heparan ... viruses interact with their native receptor CAR via the fiber knob domain We thus examined whether incorporation of PTDtat into the knob domain interfered with the Ad5-CAR interaction An enzymelinked...
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Báo cáo sinh học: " The 3'''' sequences required for incorporation of an engineered ssRNA into the Reovirus genome" pot
Ngày tải lên :
19/06/2014, 08:20
... vivo with 32P following infection of L-ST3.S2 cells and analyzed by SDS-PAGE Autoradiograms of these cells demonstrating migration rates/patterns of individual viruses are shown in the bottom panels ... the ssRNA obtained following transcription of the indicated cDNA template The fourth and bottom panel is an autoradiogram following SDS-PAGE generated by in vivo labeling with 32P of the dsRNA ... the incorporation of in vitro generated ssRNAs into new reovirus progeny viral particles This work identifies the 3' downstream flanking sequence required to ensure incorporation of an engineered...
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Báo cáo hóa học: " Genetic incorporation of the protein transduction domain of Tat into Ad5 fiber enhances gene transfer efficacy" ppt
Ngày tải lên :
20/06/2014, 01:20
... higher cell-binding activity in almost all of the cells we examined, including both highCAR and low-CAR containing cells Shown in Fig are results obtained in two representative cell lines: high-CAR ... interactions of HIV-I Tat protein with size-defined heparin oligosaccharides J Biol Chem 1999, 274(40):28198-28205 Spillmann D, Witt D, Lindahl U: Defining the interleukin-8-binding domain of heparan ... viruses interact with their native receptor CAR via the fiber knob domain We thus examined whether incorporation of PTDtat into the knob domain interfered with the Ad5-CAR interaction An enzymelinked...
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Báo cáo y học: "CXCL12 is displayed by rheumatoid endothelial cells through its basic amino-terminal motif on heparan sulfate proteoglycans" ppsx
Ngày tải lên :
09/08/2014, 07:20
... The capacity of cultured RA ECs to bind biotinylated CXCL12α was similar to that of OA ECs but in contrast to that of non -synovial ECs (HUVECs), in which constitutive binding of biotinylated CXCL12α ... vessels (nine of nine tissues) in contrast to OA vessels, in which it was only rarely (three of eight tissues) and weakly detected (Figure 1) HSPGs were detected with a diffuse pattern involving all ... Several observations have indicated the importance of chemokine docking on the luminal surface of ECs on the chemokine activation of firm adhesion and transendothelial migration of circulating...
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