cd123 and cd11c sf dcs are efficient apcs

... involved are multiple and complex, and further investigation should be pursued to help gain a better understanding of the signaling cascades governing the interactions between FGF2, MMP-13 and BMP7 ... analysis and interpretation of the data, and manuscript preparation EJT participated in the study design and manuscript preparation DKP and RKU participated in the acquisition of tissues and helped ... Alginate beads and monolayers were made for long-term and short-term analysis, respectively For alginate bead cultures, isolated NP cells and AF cells were resuspended in 1.2% alginate, and beads...

Ngày tải lên: 09/08/2014, 10:23

... hydrazone (PIH) and its analogue, 2-hydroxy-1-naphthylaldehyde isonicotinoyl hydrazone (311), were synthesized by standard techniques [34] Both PIH and 311 are strong Febinding ligands [34] and were ... concentrations of both physiological chelators (apolactoferrin and apoTf) and synthetic chelators (EDTA and DTPA) were used, and for all ligands no significant potentiation of 59Fe mobilization was ... (0.01–1 mM) The media and cells were separated and the 59Fe levels in each assessed Cells were lysed and the cytosols subjected to native PAGE-59Fe-autoradiography (see Materials and methods) (B)...

Ngày tải lên: 08/03/2014, 22:20

... two, or three glycosylated residues (Fig 2) Thus, peaks (b) and (c) apparently contained two GalNAc residues and peaks (d) and (e) apparently contained a single GalNAc residue Peak (a) appears ... residues, namely, peaks (c) and (e), was unsuccessful because of their minute quantity According to their retention times, peaks (e) and (c) are likely to contain FITC– PTTT*PLK and FITC–PT*TT*PLK, respectively, ... pp-GalNAc-T1, T2, and T3 were prepared as described previously [43] Briefly, each of the plasmids pAcGP67-GalNAc-T1-sol, pAcGP67-GalNAcT2-sol, and pAcGP67-GalNAc-T3-sol were cotransfected with Baculo-Gold...

Ngày tải lên: 17/03/2014, 10:20

... generation and oxidative stress with ageing and exercise: differential effects in the myocardium and liver Acta Physiol Scand 169, 343–351 Møller P & Loft S (2006) Dietary antioxidants and beneficial ... compounds are shown in Fig The 50% inhibitory concentration (IC50) of each compound is shown in Table Selenourea A and selenourea B significantly inhibited CL as compared with selenoamide A and selenoamide ... selenoureas and selenoamides using three cell lines, HaCaT, HEL, and HEp-2, and PMNs, by the microtiter tetrazolium method [41–43] The cytotoxicities of the compounds in each cell line are shown...

Ngày tải lên: 23/03/2014, 07:20

... antiserum 1311C as a diffuse band of 42–44 kDa (Fig 2, lanes 11 and 12) With antisera 1311N and 1311NC, we could not precipitate this product (Fig 2, lanes and 3, and 20 an 21), possibly because ... antisera (1311N, 1311NC and 1311C) immunoprecipitated this fragment in its deglycosylated forms, namely as proteins of  23 and  24 kDa (Fig 2, lanes 5, 14 and 23, and 6, 15 and 24, respectively) ... POMC that bound nonspecifically (asterisk) Ó FEBS 2002 h and h, giving rise to products of  61 and  63 kDa (Fig 2, compare lane with and 10 with 11) This minor shift in mobility is likely due...

Ngày tải lên: 31/03/2014, 09:20

... Vpr and p24 levels (data not shown) The results indicate that comparable levels of Vpr and p24 were observed in Discussion HIV-1 and are members of lentivirus family of retroviruses and are grouped ... antibody Similarly, cells were pelleted and cell lysates were used to detect the presence and expression of Gag and Vpr in transfected culture Results Construction and characterization of chimeric Vpr ... Vpr) and compared with BiFC by microscopy (Fig 4C) Results indicate that Vprwt, E48A and R80A exhibited 35, 22 and 39% BiFC positive cells respectively, whereas mutants A30L, Δ44, L68E and H71R...

Ngày tải lên: 12/08/2014, 04:20

... were lysed in hypotonic TE buffer, and the S1 fractions were either untreated (lane and 2) or treated with 0.5% Triton X-100 at 4°C (lane and 4) and 37°C (lane and 6), respectively Following the ... using a site-directed mutagenesis kit (Stratagene) and confirmed by sequencing Cell, transfection and virus purification The culture and transfection of HEK-293T cells with these plasmids using ... JM, XYL and JX have made equal contributions to conception and design, acquisition of data, and analysis and interpretation of data JM was involved in drafting the manuscript QZ, ZLL and PPJ...

Ngày tải lên: 13/08/2014, 01:20

... transmembrane protein [26,27] and is expressed by a wide-range of human and animal cells [28,29] Mouse and human pDCs [30,31] and human monocyte-derived DCs (MDDCs) [29] express endogenous tetherin, ... between tetherin and the inhibition of HIV-1 replication and cell-to-cell transmission in DCs by assessing the levels of tetherin expression in iDCs and mature DCs We observed that iDCs were extremely ... tetherin expression in DCs, DCs from different donors were treated with IFNa and LPS and analyzed for tetherin expression on the surface and in whole cell lysates by flow cytometry and immunoblotting,...

Ngày tải lên: 13/08/2014, 01:20

... Sertoli cells are bFGF producing cells [2,33] and are the target of this growth factor as bFGF inhibits FSH-induced estradiol synthesis ([5], and our results) HSPG and especially glypicans and syndecans ... ngáml)1), FSH (100 ngáml)1) and/ or Ro-201724 (250 lM) and/ or bFGF (5 ngáml)1) Estradiol production was determined by radioimmunoassay Values are expressed in pg per 106 cells and are representative of ... (100 ngámL)1) and/ or bFGF (5 ngámL)1) and/ or sodium chlorate (10 mM) during 24 h Estradiol production was determined by radioimmunoassay Values are expressed in pg per 106 cells and are representative...

Ngày tải lên: 21/02/2014, 03:20

... [40,41], and they also contained human apoE Extensive previous studies in both humans and experimental animals have shown that model particles of this type are cleared from the blood and metabolized ... in our laboratory and by others has demonstrated that chylomicron remnants are able to induce foam cell formation in human monocyte-derived macrophages (HMDM) [21] and in human and murine macrophage ... Characteristics of CRLPs and pCRLPs The lipid content of the CRLPs and pCRLPs used is shown in Table The total cholesterol, triacylglycerol and phospholipid content, and the triacylglycerol/total...

Ngày tải lên: 16/03/2014, 16:20

... 3.0) Peaks in the transformed spectra are indicated, and their observed masses and compositions are indicated in the key shown below Weak peaks in the spectra at higher masses are presumed to be ... in the cases of GFP and hCypA and mostly soluble protein for HMP and PpiB Given that the chromophore in GFP involves a tyrosine (Tyr66), and that its photophysical properties are particularly sensitive ... in the fluorophore, two of which (Tyr92 and Tyr106) are completely buried in the structure hCypA and PpiB are highly homologous proteins that contain two and three tyrosine residues, respectively...

Ngày tải lên: 16/03/2014, 22:20

... electrophoresis, both these bands either disappeared or were strongly reduced, and new bands with lower Mr appeared (Fig 3A, lane 2) Two weak bands at 80 and 85 kDa were seen along with a strong doublet ... degradation and removal of CS-chains from the complex resulted in the disappearance or a large reduction of the bands in the stacking gel and the band at Ó FEBS 2003 300 kDa, and new bands around ... R-250 (30% methanol) and destained in a solution containing 30% methanol and 10% acetic acid Gelatinase activity was evident as cleared (unstained) regions The area of the cleared zones was analyzed...

Ngày tải lên: 17/03/2014, 10:20

... peroxinitritemediated oxidative and/ or protein nitration events The interest in oxidative biology in many areas of health care has reinforced efforts to understand mechanisms of cell injury and death during ... to compare the abilities of tyrosinated and nitrotyrosinated tubulin to assemble into and to disassemble from microtubules, by monitoring radioactivity and immunoreactivity (see Materials and methods) ... collected 15 days after each injection and tested for affinity and specificity and stored at )20 °C Mouse monoclonal antibodies against Tyr-tubulin (Tub1A2) and total a-tubulin (DM1A), peroxidase-conjugated...

Ngày tải lên: 17/03/2014, 10:20

... lipoprotein from LolA and somehow incorporates it into the outer membrane The overall structures of LolA and LolB are very similar [4] They comprise 11 antiparallel b-strands, which fold into ... b-barrel, and two loops covering the barrel The barrel and the loops containing three a-helices form a hydrophobic cavity, which has recently been found to undergo opening and closing on binding and ... immunodetection system, as described in (B) The densities of the bands were determined and plotted as a function of the amounts of LolB and mLolB LolB and mLolB (each lg) were analysed by SDS–PAGE, followed...

Ngày tải lên: 23/03/2014, 05:22

... 7A, reruns of the purified quail (lanes and 4), chicken (lanes and 5) and guinea fowl (lanes and 6) ZP1 proteins showed a dominant band after western blotting and probing using antiZP1 serum (lanes ... blotting and probed with anti-DIG immunoglobulin (Fig 2B), immunoreactive bands migrating at around 175 and 97 kDa, which corresponds to the apparent molecular mass values of dimeric and monomeric ... light and 10 h darkness and were provided with tap water and a commercial diet (Tokai-Kigyo, Toyohashi, Japan) ad libitum The birds were killed by bleeding from the carotid artery, and the serum and...

Ngày tải lên: 23/03/2014, 07:20

... receptor CAR, and delivered transgenes into both high-CAR and low-CAR cells more efficiently than the unmodified Ad5 vector Our data further showed Ad5.PTDtat infected cells via both CAR and PTDtat ... the design of the study and manuscript preparation HW conceived of the study, participated in its design and coordination, and drafted the manuscript All authors read and approved the final manuscript ... Indeed, both genetic and non-genetic strategies have been shown to successfully retarget Ad5 vectors For example, bi-specific adapter proteins that bind both the knob domain and an alternative...

Ngày tải lên: 18/06/2014, 18:20

... molecules are the (+) strand and function as mRNA Each of the virions' RNA transcripts can code for the synthesis of at least one polypeptide Twelve reovirus-specific polypeptides are synthesized ... gene and protein σ2 to study the function of this protein and its role in reovirus replication and host cell interaction Additionally, it will now be possible to use reovirus as a vaccine and ... are displayed autoradiogram panels Within each panel, the ssRNA, dsRNA and CAT dsRNA panels are Northerns analyzing RNA extracted from cells lipofected 12 hours earlier with wildtype ssRNAs and...

Ngày tải lên: 19/06/2014, 08:20

... receptor CAR, and delivered transgenes into both high-CAR and low-CAR cells more efficiently than the unmodified Ad5 vector Our data further showed Ad5.PTDtat infected cells via both CAR and PTDtat ... the design of the study and manuscript preparation HW conceived of the study, participated in its design and coordination, and drafted the manuscript All authors read and approved the final manuscript ... Indeed, both genetic and non-genetic strategies have been shown to successfully retarget Ad5 vectors For example, bi-specific adapter proteins that bind both the knob domain and an alternative...

Ngày tải lên: 20/06/2014, 01:20

... authors declare that they have no competing interests 13 Authors' contributions BS performed flow cytometry and immunofluorescence studies, FB and FAS prepared the peptides and antibodies used and contributed ... natural ligand of the G-protein-coupled receptor CXCR4 and exhibits both homeostatic and proinflammatory functions in humans The main cellular sources of CXCL12 are resident stromal fibroblasts and ... contributed to drafting and reviewing the manuscript, GP and IGC contributed to the collection and culture of human EC lines, JCR participated in the design of the study and data analysis, and JLP conceived...

Ngày tải lên: 09/08/2014, 07:20