cd11c and cd123 dcs in ra sf

... pulposus cells and (b) annulus fibrosus cells isolated from bovine intervertebral disc were cultured for days in 1.2% alginate in serum-free medium with mini-insulin–transferrin–selenium (control) ... of FGF2 in the spine disc Interestingly, Valverde-Franco and colleagues found that, in the absence of signaling from FGFR3, a compensatory increase in interaction is seen between FGF2 and FGFR1, ... JA: Aging and degeneration of the human intervertebral disc Spine 1995, 20:1307-1314 Freemont TJ, LeMaitre C, Watkins A, Hoyland JA: Degeneration of intervertebral discs: current understanding...

Ngày tải lên: 09/08/2014, 10:23

... Determination of intracellular iron distribution using native-PAGE-59Fe-autoradiography Native-PAGE-59Fe-autoradiography was performed using standard techniques in our laboratory [38] Bands on X-ray ... mechanism(s) involved in the intracellular trafficking and delivery of Fe to ferritin remain unclear, although intermediates of low Mr [46] or high Mr (e.g metal-binding chaperones) [47] could be involved ... incorporation into ferritin gradually increasing as a function of reincubation time (Fig 3B) In contrast, in cells treated with GSNO, ferritin-59Fe levels decreased after 90 of reincubation In fact,...

Ngày tải lên: 08/03/2014, 22:20

... H & Irimura, T (1999) Incorporation of N-acetylgalactosamine into consecutive threonine residues in MUC2 tandem repeat by recombinant human N-acetyl-D-galactosamine transferase-T1, T2 and T3 FEBS ... 0.05%) in the linear mode with the delayed extraction setting Recorded data were processed by using GRAMS/386 software Amino acid sequencing Pulsed liquid Edman degradation amino acid sequencing ... flanking amino acid sequences surrounding the Thr residue It has not been demonstrated that the order of GalNAc incorporation into three Thr and/ or Ser residues in the vicinity is strictly determined...

Ngày tải lên: 17/03/2014, 10:20

... antinociceptive, neuroprotective and anti -in ammatory properties in different experimental models [30–35] Ebselen inhibits leukocyte in ltration and activation, leading to elimination of H2O2 in ... (2007) Free radicals and antioxidants in normal physiological functions and human disease Int J Biochem Cell Biol 39, 44–84 Flora SJ (2007) Role of free radicals and antioxidants in health and disease ... exercise enhance free radical generation in rat skeletal muscle J Appl Physiol 87, 465–470 Bejma J, Ramires P & Ji LL (2000) Free radical generation and oxidative stress with ageing and exercise: differential...

Ngày tải lên: 23/03/2014, 07:20

... from adopting its normal conformation or from interacting with its binding partner Tunicamycin inhibits N-linked glycosylation and cleavage of intact Ac45 As N-linked glycosylation of intact Ac45 ... antisera raised against more C-terminally located regions of Ac45 (1311C and 1311NC, Figs and 2, lanes 10–15 and 19–24) Finally, the N-terminal Ac45 fragment contains one potential N-linked glycosylation ... antisera 1311C and 1311N, directed against a synthetic peptide comprising the 12 C-terminal amino-acid residues of Xenopus Ac45 and against a recombinant fragment of Xenopus Ac45 (comprising amino-acid...

Ngày tải lên: 31/03/2014, 09:20

... virion incorporation are defective in Gag interaction and virion incorporation Figure Role of Vpr oligomerization in incorporation of Vpr into virus particles (A) Visualization of Vprwt and Gag interaction ... acids in the N terminal region including the helical domains are essential for stability and virion incorporation and a region comprising the Helix III and the C terminal region determines the ... plasmid transfection suggesting that Vpr-Gag interaction is specific as reported previously [26,40] Subcellular distribution of Gag-Gag interaction and Gag-Vpr interaction resulted in cytosolic and...

Ngày tải lên: 12/08/2014, 04:20

... gradients, hA3G in the S1 was found in both LMM fractions (including fractions and 4) and HMM fractions (including fractions and 8), as shown in the top panel of Figure 1B hA3G in the P100 was ... anti-Caveolin-1 (lipid raft marker), anti-membrane transferrin receptor (TFR, a cytoplasm membrane bound protein) and anti-HA As shown in Figure 2A, total HMM hA3G was fractionated into raft (lane and ... within the N-terminal part of the linker region play an important role in mediating the hA3G/Gag interaction hA3G missing in this region will not likely bind to Gag, thus abolishing its incorporation...

Ngày tải lên: 13/08/2014, 01:20

... trafficking and transmission in mature DCs Tetherin knockdown in mature DCs modestly enhances HIV-1 transmission to CD4+ T cells To examine the role of tetherin in mature DC-mediated HIV-1 transmission ... HIV-1 infection affects the level of tetherin expression in DCs, iDCs, mDC-LPS and mDC-IFNa were separately infected with WT NL(AD8) and ΔVpu, and the expression of tetherin and HIV-1 Gag in DCs ... gel (Bio-Rad) and transferred to polyvinylidine fluoride membrane (Millipore) using an Electrophoretic Transfer Cell or Semi-Dry Elecrophoretic Transfer Cell (Bio-Rad) Blots were stained with...

Ngày tải lên: 13/08/2014, 01:20

... age The bFGF binding requires speci®c motifs on highly sulfated HS chains Sodium chlorate, in inducing structural alteration of HS chains, prevents the bFGF binding Our results indicate that ... on proteins and carbohydrate residues in intact cells without inhibiting cell growth or protein synthesis [35±37], and proteoglycan sulfation in cultured Sertoli cells from 20-day-old rats [38] ... transcription factors that bind to AP-1 or FIN-1 or that inactive binding or transactivation capacity of, for example, FIN-1 and USF-1, could explain activation or inhibition of FiRE From our...

Ngày tải lên: 21/02/2014, 03:20

... lipoproteins which pass into the blood via the thoracic duct and are then rapidly metabolized by lipoprotein lipase in extrahepatic capillary beds, removing some of the triacylglycerol and leaving ... Fidge, N.H & Yin, J (1982) Specific, saturable binding and uptake of rat chylomicron remnants by rat skin fibroblasts J Lipid Res 23, 638–644 42 Redgrave, T.G., Ly, H.L., Quintao, C.R., Ramberg, C.F ... demonstrated that chylomicron remnants are able to induce foam cell formation in human monocyte-derived macrophages (HMDM) [21] and in human and murine macrophage cell lines [22–24], and that, in...

Ngày tải lên: 16/03/2014, 16:20

... naturally in humans by tyrosinase-catalysed oxidation of tyrosine in melanocytes for melanin production, and by tyrosine hydroxylase in the brain for biosynthesis of catecholamine neurotransmitters ... detects proteins containing o-catechols, like DOPA, after their separation by SDS ⁄ PAGE and western transfer to poly(vinylidene difluoride) membranes [14,17] The staining method was verified using purified ... spectra of crude mixtures containing PpiB that had been synthesized in the presence of tyrosine or DOPA using amino acid mixtures that contained both (15N)cysteine and (15N)phenylalanine in place...

Ngày tải lên: 16/03/2014, 22:20

... containing 0.1% (w/v) gelatin in both the stacking and the separating gel, and 7.5% (w/v) of polyacrylamide, respectively The gelatin zymograms were calibrated with both human gelatinase standards ... (matrix metalloproteinase-2) requires Ca2+ for fibronectin and heparin binding: binding properties of recombinant gelatinase AC domain to extracellular matrix and basement membrane components J ... obtained if the degraded CS-chains were removed or not removed from the sample, demonstrating that the CS-chains were not involved in the calcium-induced processing and release of the gelatinase...

Ngày tải lên: 17/03/2014, 10:20

... column and used to incorporate [14C]nitrotyrosine with incubation conditions as described in Materials and methods (A) Incorporation of radioactive nitrotyrosine into tubulin was determined in 0.1 ... [14C]-3-nitrotyrosine (6.7 lM, )1 450 lCiÆlmol ) was incorporated into protein (Fig 1A) Inclusion of mM nonradioactive nitrotyrosine in the incubation system led to reduced incorporation of radioactivity In ... nitrotyrosinated tubulin (and of tyrosinated tubulin for comparison) during reconstitution of microtubules from soluble rat brain extract For monitoring the tubulin isospecies, we used radioactive amino...

Ngày tải lên: 17/03/2014, 10:20

... lipoprotein from LolA and somehow incorporates it into the outer membrane The overall structures of LolA and LolB are very similar [4] They comprise 11 antiparallel b-strands, which fold into an incomplete ... present in the outer membrane and be involved in the membrane incorporation of lipoproteins However, as mLolB was found to be able to catalyse the incorporation of lipoproteins, even into liposomes, ... membrane-specific AcrA remained in the inner membrane Therefore, lipoproteins are localized in the correct membranes in the steady state, even when mLolB functions in the periplasm To examine the sorting of...

Ngày tải lên: 23/03/2014, 05:22

... binding was inhibited by incubating the membrane with 5% non-fat skim milk in saline buffered at pH 7.4 with 10 mm Tris–HCl containing 0.1% Tween 20 (blocking buffer), and the membrane was incubated ... glycoproteins (ZP1, ZP2 and ZP3) in mouse [4,8] and four glycoproteins (ZP1, ZP2, ZP3 and ZP4) in several other organisms, including humans, bonnet monkeys and rats [9–11] The ZP gene family proteins ... to Incorporation of ZP1 into perivitelline membrane demonstrate that exogenous ZP1 derived from the serum, but not from the PL, can be successfully incorporate into the PL Results Incorporation...

Ngày tải lên: 23/03/2014, 07:20

... we examined, including both highCAR and low-CAR containing cells Shown in Fig are results obtained in two representative cell lines: high-CAR expressing Hela cells, and low-CAR expressing U118MG ... viruses interact with their native receptor CAR via the fiber knob domain We thus examined whether incorporation of PTDtat into the knob domain interfered with the Ad5-CAR interaction An enzymelinked ... intrinsic to these non-genetic modification strategies, the efficiency of retargeting depended on the affinity and stability of protein-protein interactions, and thus may be highly variable in...

Ngày tải lên: 18/06/2014, 18:20

... ST3 ssRNA (obtained from in vitro transcription from reovirus cores) and 0.1 µg of the indicated s2 ssRNA (obtained from in vitro transcription using T7 RNA polymerase and the indicated cDNA ... vivo with 32P following infection of L-ST3.S2 cells and analyzed by SDS-PAGE Autoradiograms of these cells demonstrating migration rates/patterns of individual viruses are shown in the bottom panels ... Reovirus ST3 strain Dearing and reovirus ST2 strain Jones were used Both were grown in L929 mouse fibroblasts in MEM or RPMI supplemented with 5% FBS The recombinant viruses containing the CAT gene...

Ngày tải lên: 19/06/2014, 08:20

... we examined, including both highCAR and low-CAR containing cells Shown in Fig are results obtained in two representative cell lines: high-CAR expressing Hela cells, and low-CAR expressing U118MG ... viruses interact with their native receptor CAR via the fiber knob domain We thus examined whether incorporation of PTDtat into the knob domain interfered with the Ad5-CAR interaction An enzymelinked ... intrinsic to these non-genetic modification strategies, the efficiency of retargeting depended on the affinity and stability of protein-protein interactions, and thus may be highly variable in...

Ngày tải lên: 20/06/2014, 01:20

... diffuse pattern involving all vessels in both RA and OA sections Colocalization of HSPGs and CXCL12 was confirmed in RA vessels, although many vessels in RA sections and all vessels in most OA sections ... (Figure 1) In both RA and OA sections, CXCL12 was detected in lining synoviocytes and scattered fibroblasts in the sublining, although RA sections showed a higher proportion of labelled cells and some ... enhanced interactions with EC CXCR4 Consistently with previous studies in a human EC cell line [17], CXCL12α docking on RA EC membrane relies on interactions between its first β strand basic motif and...

Ngày tải lên: 09/08/2014, 07:20