cationic lipospheres as delivery systems for nucleic acid molecules

... Phenolic acids C6–C1 Salicylic acid D Acetophenones C6–C1 3-Acetyl-6-methoxybenzaldehyde E Phenylacetic acids C6–C2 p-Hydroxyphenylacetic acid F Hydroxycinnamic acids C6–C3 Caffeic acid Chlorogenic acid ... liquid form and are usually prescribed by a physician for speciÞc diseases or disorders Medical foods are primarily intended for patients in hospitals or for individuals with rare diseases and ... cited in basic nutrition texts as being necessary for the metabolism of carbohydrates for energy Thus the association: because the drink contains these vitamins, energy is made available faster Some...

Ngày tải lên: 25/10/2013, 21:20

... cellular delivery of nucleic acids have caused various problems for successful clinical gene therapy [15,16] 13 Gene delivery systems Viral gene delivery systems Viral gene delivery systems are based ... delivery systems for gene therapy of genetic or acquired human diseases has motivated the search for, and utilisation of, safer carrier molecules Non-viral delivery systems such as naked or formulated ... uptake and efficient endosomal release Besides their efficiency as pDNA delivery systems, lipid-based formulations were reported as efficient delivery systems for siRNA in vitro and in vivo [105-107]...

Ngày tải lên: 23/08/2015, 17:29

... tri-phasic burstlike profile that was absent in SBF SBF-soaked scaffolds showed sustained release of the growth factors and total release was not achieved, whereas total release was realized for ... of PCL-TCP scaffolds 164 9.1.3 PCL-TCP scaffolds as delivery systems for a single growth factor 166 9.1.4 PCL-TCP scaffolds as delivery systems for multiple growth factors 167 9.1.5 Rat femoral ... thesis showed for the first time that threedimensional, bioactive polycaprolactone scaffolds can serve effectively as protein delivery systems for bone regeneration and has the potential for clinical...

Ngày tải lên: 15/09/2015, 17:10

... designated as feasible candidates for drug delivery systems, anti-cancer agents and vaccine immunotherapy For example, DNA vaccine delivery to APCs has been facilitated by microencapsulation of plasmid ... lysosome B) peptide-based nucleic acid delivery systems: Both covalent attachment and/or non-covalent complexes of peptideDNA are acting similar to lipid-based systems The designed cationic peptides ... of California, San Diego; ID: NCT00849524] Electroporation Over the past decades, electroporation (EP) technology has remained a reliable laboratory tool for the delivery of nucleic acid molecules...

Ngày tải lên: 06/03/2014, 02:21

... suitable containers and autoclaved before use Glassware and other suitable equipment should be baked at 180°C for at least h If desired, glassware can be acid- washed prior to baking Other equipment, ... protocol for an isothermal chain reaction that makes useof the presenceof DNAprimers, T7 promoters, T7 polymerase, RNaseH, and AMV reverse transcriptase to produce large quantities of nucleic acid ... 50-mL disposable plastic tube and centrifuge at 1500g for 10 mm at room temperature to separate the two phases Remove the lower phenol phase by gentle aspiration through a Pasteur pipet attached...

Ngày tải lên: 11/04/2014, 10:14

... suitable candidates for a nanoparticle-based drug delivery system to improve their pharmacological performance For this purpose, a “longboat delivery system” has been prepared for the platinum warhead ... nanohorns for the enhancement of their dispersibility This approach is also true for CNTs [72] Polymeric drug conjugates, as a new class of systems, have been envisaged for tumor tissue-specific delivery ... of CNTs as gene carriers in gene delivery has been considered quite promising Gene therapy involves not only the gene-based treatment for cancers but also that for the infectious diseases by...

Ngày tải lên: 20/06/2014, 22:20

... light delivery systems In this thesis a new light delivery system consisting of the nano-aperture vertical-cavity surface emitting laser (VCSEL) as a potential candidate for an alternative light delivery ... hysteresis loop for a ferromagnetic material is shown in Figure 1.3 The material has zero net magnetization before applying an external magnetic field The magnetization increases as the external ... which was emerging at that time [7, 8] In 1937, Victor Atanasoff tried to use Poulsen’s magnetic drum as a memory device for computers but he could not succeed due to the inability to perform magnetic...

Ngày tải lên: 09/09/2015, 08:17

... pertussis as a live delivery system 31 1.5.2.1.4.1 Integration of foreign genes into B pertussis genome 31 1.5.2.1.4.2 Virulence factors as carriers for antigen presentation 33 1.5.3 Lactic acid bacteria ... OF EDIII AS A SUBUNIT VACCINE CANDIDATE 4.2 146 BORDETELLA PERTUSSIS AS A LIVE VECTOR FOR THE PRODUCTION AND MUCOSAL DELIVERY OF DENV2 EDIII 4.3 147 LACTOCOCCUS LACTIS AS A LIVE VECTOR FOR THE ... D2S10 [54] Vascular leakage is a hallmark of severe dengue disease in humans; development of vascular leak in AG129 therefore significantly increased the relevance of this model for the study...

Ngày tải lên: 09/09/2015, 08:18

... identified for both breast and prostate cancer, for example In contrast, fewer numbers of skin metastases than expected based on blood-flow patterns were found for osteosarcomas, stomach and testicular ... or other sites Metastases form more quickly than in spontaneous assays and in greater numbers, facilitating statistical analysis However, a drawback of experimental metastasis assays is that only ... tumor metastasis Tumor cells can hardly extravasate into the circulation system significantly until angiogenesis has been induced, and the augment in tumor associated neovasculature also increases...

Ngày tải lên: 11/09/2015, 09:58

... the malignant tumours was the increased vasculature Therefore, targeting tumour vasculature rather than the tumour cell itself as a treatment for cancer has gained increasing interest in recent ... growth which was more pronounced than the use of prodrug alone Unfortunately, for reasons unknown this inhibition in tumour growth did not persist However, it was clear that C sporogenes has a great ... oncolyticum/sporogenesrecombinant for E coli colicin E3 C beijerinckii (acetobutylicum) recombinant for E coli cytosine deaminase (CDase) C beijerinckii (acetobutylicum) recombinant for Nitroreductase (NTR) In vitro...

Ngày tải lên: 14/08/2014, 19:22

... activities CNBP was reported as single-stranded nucleic acid binding protein able to recognize RNA as well as single-stranded DNA molecules in electrophoretic mobility shift assays (EMSA) [10] ... phosphorylation on nucleic acid binding by means of EMSAs following reported protocols [7,10] We assayed two ssDNA probes representative of the informed CNBP singlestranded nucleic acid targets, the ... concentration) Except for heparin and PKI kinase phosphorylation inhibition assays, 4% (v ⁄ v) dimethylsulfoxide was added to kinase reaction mixture as control Electrophoretic mobility shift assay (EMSA)...

Ngày tải lên: 16/03/2014, 12:20

... the saturated FAs lauric acid, myristic acid (tetradecanoic acid) , palmitic acid (hexadecanoic acid) and the unsaturated FAs oleic acid [(Z)-octadec-9-enoic acid] and arachidonic acid (all-cis-5,8,11,14-eicosatetraenoic ... specificity for arachidonic acid than the already established arachidonic acid x-hydroxylase CYP4A11 [44,45] The formation of x-hydroxylated arachidonic acid (20-hydroxyeicosatetraenoic acid) plays ... x-oxidation In the case of mitochondrial FA b-oxidation disorders, there is accumulation of certain FAs either as free Fatty acid oxidation disorders FAs, or in an esterified form as in CoA and carnitine...

Ngày tải lên: 22/03/2014, 16:21

... metastases less than 250 copies/μl for CK19 were evaluated as negative result for OSNA (unpublished data) Therefore results were classified as (-) for CK 19 mRNA copies/μL less than 250 (for ... called One Step Nucleic Acid Amplification (OSNA) has recently been indicated as a fast molecular diagnostic approach for the detection of LN metastases [20,21] The aim of our study was to apply ... design for lymph node workup To compare OSNA with histology, the CK19 mRNA based OSNA procedure was performed as described elsewhere [20] CK 19 was identified as the most sensitive marker for OSNA...

Ngày tải lên: 18/06/2014, 16:20

... amplified cDNA was 434 bp PCR was performed as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) ... PCFC-g-PEI as gene delivery vectors, the inhibition effect was not obvious too (data not shown) Therefore, among the three modified PEI, the tumor was obviously suppressed when using FAPEAs as a gene delivery...

Ngày tải lên: 18/06/2014, 19:20

... amplified cDNA was 434 bp PCR was performed as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) ... PCFC-g-PEI as gene delivery vectors, the inhibition effect was not obvious too (data not shown) Therefore, among the three modified PEI, the tumor was obviously suppressed when using FAPEAs as a gene delivery...

Ngày tải lên: 20/06/2014, 03:20

... care (64% vs 44%), but not for acute care Moreover, the "differences were greatest in areas where the VHA has established performance measures and actively monitors performance." From the standpoint ... and information systems Other answers could come from comparing performance improvement for the eight topic areas created by QUERI with others not so targeted but still part of the VHA performance ... of performance measures to compare 12 community samples with 12 VA systems [2] Patients from the VHA scored significantly higher for adjusted overall quality (67% vs 51%), chronic disease care...

Ngày tải lên: 11/08/2014, 05:21

... three flasks, A, B and C Folic acid (0.01 moles, 0.28 g) was added directly to Flask B and the solution was stirred for 15 EDC (0.1 g) and DMAP (0.1 g) were added to flask C and the solution was stirred ... prepared by passing H2 Te gas through an aqueous basic solution containing Cd(ClO4)2, thioglycolic acid (TGA) stabilizer The resultant mixture was heated under reflux for hours The solution was then ... FA, a competition assay was performed with free FA In the case of QD (C) internalisation was reduced by free FA to the same level as QD (A) alone As expected the free FA molecules could block the...

Ngày tải lên: 11/08/2014, 08:20

... DNase I digestion, whereas plasmid DNA was degraded completely Armored DNA and plasmid DNA indicate samples without DNase I digestion methods, and are likely to be compatible with all HBV DNA nucleic ... in plasma The stability of armored DNA in plasma was poor The high-copy Armored DNA was not stable for more than days at 4°C, 37°C, or room temperature; the low-copy armored DNA in plasma was not ... (gt11 -for and gt11-rev), and 1.25 U of PrimerStart Taq DNA polymerase (TaKaRa, Japan) After an initial incubation at 94°C for min, 35 cycles were performed as follows: 94°C for 30 s, 58°C for 30...

Ngày tải lên: 12/08/2014, 04:21

... designed for the detection of both clades [19,20] In addition, clinical evaluation has not been performed for most of these assays [15,18-20] Recently, the locked nucleic acid (LNA) technology has ... viruses in South East Asia has highlighted the need for RT-PCR assays that allow detection of both genetic clades We developed a single step rRT-PCR assay using an LNA TaqMan probe for direct detection ... Indonesia for his support for this work, and Dr Maciej Boni from the Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Dist 05, Ho Chi Minh City, Viet Nam for...

Ngày tải lên: 12/08/2014, 04:21

... mouse Hyal2 chimeras and assayed them for receptor function (Fig 2) Hyal2 was divided into four domains based on the availability of convenient restriction enzyme sites in the cDNAs at positions ... high as those of human Hyal2 following expression plasmid Figure transfection Cell-surface mouse Hyal2 expression levels are at least as high as those of human Hyal2 following expression plasmid ... selection during the evolution of mammals for Hyal2 variants that cannot act as receptors for these viruses Evidence for such selection has been found for other proteins involved in virus defense,...

Ngày tải lên: 13/08/2014, 09:21