cationic lipospheres as delivery systems for nucleic acid molecules
Beverages as Delivery Systems for Nutraceuticals
... Phenolic acids C6–C1 Salicylic acid D Acetophenones C6–C1 3-Acetyl-6-methoxybenzaldehyde E Phenylacetic acids C6–C2 p-Hydroxyphenylacetic acid F Hydroxycinnamic acids C6–C3 Caffeic acid Chlorogenic acid ... liquid form and are usually prescribed by a physician for speciÞc diseases or disorders Medical foods are primarily intended for patients in hospitals or for individuals with rare diseases and ... cited in basic nutrition texts as being necessary for the metabolism of carbohydrates for energy Thus the association: because the drink contains these vitamins, energy is made available faster Some...
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Linear and branched chitosan oligomers as delivery systems for pDNA and siRNA in vitro and in vivo
... cellular delivery of nucleic acids have caused various problems for successful clinical gene therapy [15,16] 13 Gene delivery systems Viral gene delivery systems Viral gene delivery systems are based ... delivery systems for gene therapy of genetic or acquired human diseases has motivated the search for, and utilisation of, safer carrier molecules Non-viral delivery systems such as naked or formulated ... uptake and efficient endosomal release Besides their efficiency as pDNA delivery systems, lipid-based formulations were reported as efficient delivery systems for siRNA in vitro and in vivo [105-107]...
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The evaluation of bioactive polycaprolactone scaffolds as protein delivery systems for bone engineering applications
... tri-phasic burstlike profile that was absent in SBF SBF-soaked scaffolds showed sustained release of the growth factors and total release was not achieved, whereas total release was realized for ... of PCL-TCP scaffolds 164 9.1.3 PCL-TCP scaffolds as delivery systems for a single growth factor 166 9.1.4 PCL-TCP scaffolds as delivery systems for multiple growth factors 167 9.1.5 Rat femoral ... thesis showed for the first time that threedimensional, bioactive polycaprolactone scaffolds can serve effectively as protein delivery systems for bone regeneration and has the potential for clinical...
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Improvement of different vaccine delivery systems for cancer therapy pptx
... designated as feasible candidates for drug delivery systems, anti-cancer agents and vaccine immunotherapy For example, DNA vaccine delivery to APCs has been facilitated by microencapsulation of plasmid ... lysosome B) peptide-based nucleic acid delivery systems: Both covalent attachment and/or non-covalent complexes of peptideDNA are acting similar to lipid-based systems The designed cationic peptides ... of California, San Diego; ID: NCT00849524] Electroporation Over the past decades, electroporation (EP) technology has remained a reliable laboratory tool for the delivery of nucleic acid molecules...
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protocols for nucleic acid analysis by nonradioactive probes
... suitable containers and autoclaved before use Glassware and other suitable equipment should be baked at 180°C for at least h If desired, glassware can be acid- washed prior to baking Other equipment, ... protocol for an isothermal chain reaction that makes useof the presenceof DNAprimers, T7 promoters, T7 polymerase, RNaseH, and AMV reverse transcriptase to produce large quantities of nucleic acid ... 50-mL disposable plastic tube and centrifuge at 1500g for 10 mm at room temperature to separate the two phases Remove the lower phenol phase by gentle aspiration through a Pasteur pipet attached...
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Báo cáo hóa học: " The application of carbon nanotubes in target drug delivery systems for cancer therapies" docx
... suitable candidates for a nanoparticle-based drug delivery system to improve their pharmacological performance For this purpose, a “longboat delivery system” has been prepared for the platinum warhead ... nanohorns for the enhancement of their dispersibility This approach is also true for CNTs [72] Polymeric drug conjugates, as a new class of systems, have been envisaged for tumor tissue-specific delivery ... of CNTs as gene carriers in gene delivery has been considered quite promising Gene therapy involves not only the gene-based treatment for cancers but also that for the infectious diseases by...
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THE STUDY OF NOVEL LIGHT DELIVERY SYSTEMS FOR HEAT ASSISTED MAGNETIC RECORDING
... light delivery systems In this thesis a new light delivery system consisting of the nano-aperture vertical-cavity surface emitting laser (VCSEL) as a potential candidate for an alternative light delivery ... hysteresis loop for a ferromagnetic material is shown in Figure 1.3 The material has zero net magnetization before applying an external magnetic field The magnetization increases as the external ... which was emerging at that time [7, 8] In 1937, Victor Atanasoff tried to use Poulsen’s magnetic drum as a memory device for computers but he could not succeed due to the inability to perform magnetic...
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Development of live bacterial delivery systems for presentation of dengue EDIII to the mucosal immune system 2
... pertussis as a live delivery system 31 1.5.2.1.4.1 Integration of foreign genes into B pertussis genome 31 1.5.2.1.4.2 Virulence factors as carriers for antigen presentation 33 1.5.3 Lactic acid bacteria ... OF EDIII AS A SUBUNIT VACCINE CANDIDATE 4.2 146 BORDETELLA PERTUSSIS AS A LIVE VECTOR FOR THE PRODUCTION AND MUCOSAL DELIVERY OF DENV2 EDIII 4.3 147 LACTOCOCCUS LACTIS AS A LIVE VECTOR FOR THE ... D2S10 [54] Vascular leakage is a hallmark of severe dengue disease in humans; development of vascular leak in AG129 therefore significantly increased the relevance of this model for the study...
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Drug delivery systems for antiangiogenesis and anticancer activities
... identified for both breast and prostate cancer, for example In contrast, fewer numbers of skin metastases than expected based on blood-flow patterns were found for osteosarcomas, stomach and testicular ... or other sites Metastases form more quickly than in spontaneous assays and in greater numbers, facilitating statistical analysis However, a drawback of experimental metastasis assays is that only ... tumor metastasis Tumor cells can hardly extravasate into the circulation system significantly until angiogenesis has been induced, and the augment in tumor associated neovasculature also increases...
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Báo cáo sinh học: " Clostridial spores as live ''''Trojan horse'''' vectors for cancer gene therapy: comparison with viral delivery systems" pptx
... the malignant tumours was the increased vasculature Therefore, targeting tumour vasculature rather than the tumour cell itself as a treatment for cancer has gained increasing interest in recent ... growth which was more pronounced than the use of prodrug alone Unfortunately, for reasons unknown this inhibition in tumour growth did not persist However, it was clear that C sporogenes has a great ... oncolyticum/sporogenesrecombinant for E coli colicin E3 C beijerinckii (acetobutylicum) recombinant for E coli cytosine deaminase (CDase) C beijerinckii (acetobutylicum) recombinant for Nitroreductase (NTR) In vitro...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... activities CNBP was reported as single-stranded nucleic acid binding protein able to recognize RNA as well as single-stranded DNA molecules in electrophoretic mobility shift assays (EMSA) [10] ... phosphorylation on nucleic acid binding by means of EMSAs following reported protocols [7,10] We assayed two ssDNA probes representative of the informed CNBP singlestranded nucleic acid targets, the ... concentration) Except for heparin and PKI kinase phosphorylation inhibition assays, 4% (v ⁄ v) dimethylsulfoxide was added to kinase reaction mixture as control Electrophoretic mobility shift assay (EMSA)...
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Báo cáo khoa học: Fatty acid omega-oxidation as a rescue pathway for fatty acid oxidation disorders in humans pot
... the saturated FAs lauric acid, myristic acid (tetradecanoic acid) , palmitic acid (hexadecanoic acid) and the unsaturated FAs oleic acid [(Z)-octadec-9-enoic acid] and arachidonic acid (all-cis-5,8,11,14-eicosatetraenoic ... specificity for arachidonic acid than the already established arachidonic acid x-hydroxylase CYP4A11 [44,45] The formation of x-hydroxylated arachidonic acid (20-hydroxyeicosatetraenoic acid) plays ... x-oxidation In the case of mitochondrial FA b-oxidation disorders, there is accumulation of certain FAs either as free Fatty acid oxidation disorders FAs, or in an esterified form as in CoA and carnitine...
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Báo cáo hóa học: "One Step Nucleic Acid Amplification (OSNA) - a new method for lymph node staging in colorectal carcinomas" docx
... metastases less than 250 copies/μl for CK19 were evaluated as negative result for OSNA (unpublished data) Therefore results were classified as (-) for CK 19 mRNA copies/μL less than 250 (for ... called One Step Nucleic Acid Amplification (OSNA) has recently been indicated as a fast molecular diagnostic approach for the detection of LN metastases [20,21] The aim of our study was to apply ... design for lymph node workup To compare OSNA with histology, the CK19 mRNA based OSNA procedure was performed as described elsewhere [20] CK 19 was identified as the most sensitive marker for OSNA...
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Báo cáo sinh học: "Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" doc
... amplified cDNA was 434 bp PCR was performed as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) ... PCFC-g-PEI as gene delivery vectors, the inhibition effect was not obvious too (data not shown) Therefore, among the three modified PEI, the tumor was obviously suppressed when using FAPEAs as a gene delivery...
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báo cáo hóa học:" Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" pot
... amplified cDNA was 434 bp PCR was performed as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) ... PCFC-g-PEI as gene delivery vectors, the inhibition effect was not obvious too (data not shown) Therefore, among the three modified PEI, the tumor was obviously suppressed when using FAPEAs as a gene delivery...
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Implementation Science Debate BioMed Central Open Access Lessons for non-VA care delivery systems pdf
... care (64% vs 44%), but not for acute care Moreover, the "differences were greatest in areas where the VHA has established performance measures and actively monitors performance." From the standpoint ... and information systems Other answers could come from comparing performance improvement for the eight topic areas created by QUERI with others not so targeted but still part of the VHA performance ... of performance measures to compare 12 community samples with 12 VA systems [2] Patients from the VHA scored significantly higher for adjusted overall quality (67% vs 51%), chronic disease care...
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Báo cáo khoa hoc:" Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics" pdf
... three flasks, A, B and C Folic acid (0.01 moles, 0.28 g) was added directly to Flask B and the solution was stirred for 15 EDC (0.1 g) and DMAP (0.1 g) were added to flask C and the solution was stirred ... prepared by passing H2 Te gas through an aqueous basic solution containing Cd(ClO4)2, thioglycolic acid (TGA) stabilizer The resultant mixture was heated under reflux for hours The solution was then ... FA, a competition assay was performed with free FA In the case of QD (C) internalisation was reduced by free FA to the same level as QD (A) alone As expected the free FA molecules could block the...
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Báo cáo y học: " Nuclease-resistant double-stranded DNA controls or standards for hepatitis B virus nucleic acid amplification assays" pptx
... DNase I digestion, whereas plasmid DNA was degraded completely Armored DNA and plasmid DNA indicate samples without DNase I digestion methods, and are likely to be compatible with all HBV DNA nucleic ... in plasma The stability of armored DNA in plasma was poor The high-copy Armored DNA was not stable for more than days at 4°C, 37°C, or room temperature; the low-copy armored DNA in plasma was not ... (gt11 -for and gt11-rev), and 1.25 U of PrimerStart Taq DNA polymerase (TaKaRa, Japan) After an initial incubation at 94°C for min, 35 cycles were performed as follows: 94°C for 30 s, 58°C for 30...
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Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... designed for the detection of both clades [19,20] In addition, clinical evaluation has not been performed for most of these assays [15,18-20] Recently, the locked nucleic acid (LNA) technology has ... viruses in South East Asia has highlighted the need for RT-PCR assays that allow detection of both genetic clades We developed a single step rRT-PCR assay using an LNA TaqMan probe for direct detection ... Indonesia for his support for this work, and Dr Maciej Boni from the Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Dist 05, Ho Chi Minh City, Viet Nam for...
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Báo cáo y học: " Amino acid residues that are important for Hyal2 function as a receptor for jaagsiekte sheep retrovirus" potx
... mouse Hyal2 chimeras and assayed them for receptor function (Fig 2) Hyal2 was divided into four domains based on the availability of convenient restriction enzyme sites in the cDNAs at positions ... high as those of human Hyal2 following expression plasmid Figure transfection Cell-surface mouse Hyal2 expression levels are at least as high as those of human Hyal2 following expression plasmid ... selection during the evolution of mammals for Hyal2 variants that cannot act as receptors for these viruses Evidence for such selection has been found for other proteins involved in virus defense,...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose