... to stemcells In the first, stemcells themselves are implanted in a diseased or injured organ in the hope that they will give rise to the mature cells needed by that organ In the second, thestem ... fat cells, various brain and nervous system cells, insulin-producing cells of the pancreas, bone cells, hematopoietic cells, yolk sac, endothelial cells, primitive endodermal cells, and smooth and ... millimeter) The outer layer of the blasotocyst is called the trophoblast, andthe cluster of cells inside the sphere is called the inner cell mass At this stage, there are about 70 trophoblast cells and...
... human embryonic blood stemcells Blood 96, 1740 - 1747 IItskovitz - Eldor, J and et al (2000).; Differentiation of human embryonic stemcells into embryoid bodies comprising the three embryonic ... J A., et al (1998); Embryonic stemcells lines derived from human blastocysts Science 282, 1145 - 1147 12 Raisman, G., (2001); Olfactory ensheathing cells - another miracle cure for spinal cord ... 814 - 822 Jones, G M., et al (2000); Human embryonic stemcells technology Semin Reprod Med 18, 219 - 223 Orlic D et al (2001); Bone marrow cells regenerate infarcted myocardium nature 410, 701...
... All the patients underwent radical surgery, and none of them had undergone either chemotherapy or radiotherapy before the collection of the samples The histopathologic characteristics of the ... MCCstroma, the count of tryptase-positive mast cells in thecancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ... MCCstroma, the count of tryptase-positive mast cells in thecancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ...
... normal stemcells to cancerstemcells Adult normal stemcells represent one purported origin of cancerstemcells What causes the transformation from normal stemcells to cancerstem cells? ... isolated cancerstemcells may be more accurately referred to as cancer stemloids (or stem cell-like cancer cells) (Blagosklonny, 2007) Although these cells may exist only as a minority within thecancer ... cancerstemcells Is the genome inherently unstable in stem cells? If the answer is yes, cancerstemcells may have two reasons for having an unstable genome: being a stem cell as well as a cancer...
... communication and adhesion was associated with thecancerstem cell population These expression products have been implicated in tissue integrity [30] andthe normal stem cell 'niche' [31,32] The gene ... primitive cancercells PTL induces apoptosis in primitive cancercells Percent viability of prostate cancercellsandcells from a patient with BPH treated with increasing concentrations of PTL Cells ... of thecancerstem cell expression signature Functional annotation of thecancerstem cell expression signature (a,b) Functional concepts over-represented in cancer relative to BPH within the stem...
... ischemic, stemcells in circulation are adherent to the vascular endothelial cells, cross the endothelial cells, migrate and finally reached at ischemic sites Inflammatory may be observed in the local ... culture, the medium was refreshed, and then the medium changed every other day Cell passaging was performed when cell confluence reached 85% The purity and immunophenotype of MSCs and their potentials ... 1B) The infection rate was over 70% Flow cytometry showed the MSCs had no expressions of CD34, CD45 and HLA-DR, but high expressions of CD29 and CD44 Furthermore, the purity of cells with these...
... A549 cells, further supporting its use as a model for endothelial cells In EA.hy.926 cells, cPLA2-a was seen to relocate to structures resembling the ER andthe inner and outer surfaces of the ... in endothelial cells A S Grewal et al B C Fig The location and relocation of cPLA2-a in Triton X-100- and digitonin-permeabilized cells (A) Cells were grown on coverslips overnight and either directly ... phospholipase A2 is in the nucleus of subconfluent endothelial cells but confined to the cytoplasm of confluent endothelial cellsand redistributes to the nuclear envelope and cell junctions upon...
... All the patients underwent radical surgery, and none of them had undergone either chemotherapy or radiotherapy before the collection of the samples The histopathologic characteristics of the ... MCCstroma, the count of tryptase-positive mast cells in thecancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ... MCCstroma, the count of tryptase-positive mast cells in thecancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ...
... receptors and ligands of the IGF system Purple represents the alpha and beta subunit of IGF-1R; red represents the alpha and beta subunit of the IR-B; orange represents the alpha and beta subunit of the ... breast cancercellsand fibroblast from the surrounding stroma It has many advantages compared to the standard 3D culture: the culture volume andthe number of needed cells are smaller than in standard ... been the backbone of cancer research They have provided the understanding of systems at the molecular and cellular levels Cell lines are used in the vast majority of research labs to understand the...
... developments and ES cells would be isolated from the ICMs of the cloned preimplantation embryo When applied in a therapeutic setting, these cells would carry the nuclear genome of the patient; therefore, ... the body and large numbers of ES cells can be relatively easily obtained in vitro culture Therefore, ES cells could be the choice of cells in stem cell therapy for various diseases One of the critical ... cellsand cartilage-forming cells) A potential advantage of using adult stemcells is that the patient's own cells could be expanded in culture and then reintroduced into the patient without immune...
... oocytes and donor cells from slaughtered ovaries Therefore, we attempted to optimize autologous pig SCNT by examining the effects of electrical fusion and donor cell type on cell fusion andthe subsequent ... Preparation of donor cells Four types of somatic cells were used as donor cells: 1) denuded cumulus cells (DCCs); 2) cultured cumulus cells (CCCs); 3) cultured follicular cells (CFCs); and 4) adult skin ... is not clear whether this discrepancy between the results of the present study and those of the previous study is due to species difference The fusion rate of uncultured cumulus cells (DCCs) was...
... adenocarcinoma (HE × 200) The glands are lined by neoplasticcells with hyperchromatic nuclei The tumor was accompanied with small mucinous lakes lind by well-differentiated epithelial cellsThe tumor invades ... Oncology ColonCancer V.3.2009 Compton CC, Greene FL: The staging of colorectal cancer: 2004 and beyond Ca Cancer J Clin 2004, 54:295-308 International Union Against Cancer (UICC): Colon and rectum ... infiltrating the rectum muscularis propria from outside Core needle biopsy demonstrated the presence of a mucus producing adenocarcinoma with the same histological futures with the primary tumor and therefore...
... Isolation, culture and cell surface epitope characterisation of IPFP cellsThecells isolated from the IPFP proliferated in culture and reached confluence by day 14 The yield of cells by the end of passage ... CD105 (markers for mesenchymal stem cells) , and for CD29 (β1 integrin) Thecells stained poorly for LNGFR and STRO-1 (markers on freshly isolated bone marrow stem cells) and sparsely for 3G5 (marker ... cytometry of the IPFP cell population showed a similar staining pattern to that of bone marrow-derived stem cells, and although they stained poorly for STRO-1 and for LNGFR, the expression of these...
... give a higher yield of stemcellsand there is reduced pain and morbidity associated with the harvest of cells [8] In preliminary work, we identified perivascular cells in the IPFP tissue which ... [20] Materials and methods The defining properties of stemcells are self-renewal and multipotency Unfortunately, these crucial properties in adult stemcells show donor variability and may become ... (endothelial cell marker) stained endothelial cells in the tunica intima (Figure 1c,d) and anti-αSMA (smooth muscle cell marker) stained cells in the tunica media, forming the muscular wall of the...
... Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stemcells (AD-MSC) and annulus cells compared with separate culture of AD-MSC and annulus cells alone Data from ... contributions HEG and ENH conceived the study and participated in its design and co-ordination HT and HEG wrote the manuscript HT, MK and RD performed all experiments and assays HT and RD retrieved ... either cell type enhancing the overall ECM production, or by modification of the microenvironment of the 3D matrix through deposition of ECM components by either the AD-MSC or the annulus cells...
... formation by the transformed P63+ basal /stem cells in the metastatic loci The data evidently demonstrated both cancer cell andstem cell characteristics of the transformed P63+ basal /stem cells, supporting ... formation and evaluate candidate therapeutic agents [23,24] In this study, we used the Hh overexpression mouse model to elucidate whether the PCSCs arise from P63+ basal /stem cellsand to examine whether ... prostatic cancerstemcells [20] However, the tumorigenic activation of basal/ stemcellsand their progression toward a metastatic status under the influence of Hh signalling remain to be further...
... AK1C3 [19] and CD44 [20] have been proposed to be the markers of cancerstemcells Their expression in tumor cells could correlate with their aggressive biological behavior, drug resistance and poor ... and clinical data to assess the precise function of these marker candidates and confirm them as real biomarkers, this proteomic analysis was effective to detect them and will be applied to other ... and SCLC, 93 in the overlap only between LCNEC and LCC, and 453 in the overlap among three groups; SCLC contained a total of 1,096 including 362 unique, 100 in the overlap only between SCLC and...
... enriched for characteristics of stemcells compared to the non-SP cells, we further characterized the SP cells from the fetal liver cellsand HCC cells by analyzing the presence of markers known ... long-lived somatic stemcells Therefore, markers of “normal” stemcells are being sought with the expectation that these molecules are also expressed by cancer Page of 10 stem cells, and can be used ... liver cellsand in primary rodent HCC cancercells induced by chemical carcinogens Since the HCC cancercellsand fetal liver cells used in our study originated from the same inbred rat strain, the...