cancer stem cells and the microenvironment

... to stem cells In the first, stem cells themselves are implanted in a diseased or injured organ in the hope that they will give rise to the mature cells needed by that organ In the second, the stem ... fat cells, various brain and nervous system cells, insulin-producing cells of the pancreas, bone cells, hematopoietic cells, yolk sac, endothelial cells, primitive endodermal cells, and smooth and ... millimeter) The outer layer of the blasotocyst is called the trophoblast, and the cluster of cells inside the sphere is called the inner cell mass At this stage, there are about 70 trophoblast cells and...

Ngày tải lên: 15/03/2014, 09:20

... human embryonic blood stem cells Blood 96, 1740 - 1747 IItskovitz - Eldor, J and et al (2000).; Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic ... J A., et al (1998); Embryonic stem cells lines derived from human blastocysts Science 282, 1145 - 1147 12 Raisman, G., (2001); Olfactory ensheathing cells - another miracle cure for spinal cord ... 814 - 822 Jones, G M., et al (2000); Human embryonic stem cells technology Semin Reprod Med 18, 219 - 223 Orlic D et al (2001); Bone marrow cells regenerate infarcted myocardium nature 410, 701...

Ngày tải lên: 02/04/2014, 22:20

... Telmer and Eva Turley Breast Cancer Stem Cells 235 Chapter 11 The Microenvironment of Breast Cancer Stem Cells Deepak Kanojia and Hexin Chen Chapter 12 Involvement of Mesenchymal Stem Cells in ... receptors and ligands of the IGF system Purple represents the alpha and beta subunit of IGF-1R; red represents the alpha and beta subunit of the IR-B; orange represents the alpha and beta subunit of the ... for their growth and the role of oestrogen receptors In this study, the co-culture of MCF-7 and MDA-MB-231 (respectively ER+ and 30 Breast Cancer – Focusing Tumor Microenvironment, Stem Cells and...

Ngày tải lên: 27/06/2014, 17:20

... tumor Cancer stem cells express known prostate cancerassociated genes The cancer phenotype was validated by confirming the expression levels of several established prostate cancer markers from the ... primitive cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells ... of the cancer stem cell expression signature Functional annotation of the cancer stem cell expression signature (a,b) Functional concepts over-represented in cancer relative to BPH within the stem...

Ngày tải lên: 14/08/2014, 08:21

... ischemic, stem cells in circulation are adherent to the vascular endothelial cells, cross the endothelial cells, migrate and finally reached at ischemic sites Inflammatory may be observed in the local ... culture, the medium was refreshed, and then the medium changed every other day Cell passaging was performed when cell confluence reached 85% The purity and immunophenotype of MSCs and their potentials ... 1B) The infection rate was over 70% Flow cytometry showed the MSCs had no expressions of CD34, CD45 and HLA-DR, but high expressions of CD29 and CD44 Furthermore, the purity of cells with these...

Ngày tải lên: 25/10/2012, 11:18

... All the patients underwent radical surgery, and none of them had undergone either chemotherapy or radiotherapy before the collection of the samples The histopathologic characteristics of the ... MCCstroma, the count of tryptase-positive mast cells in the cancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ... MCCstroma, the count of tryptase-positive mast cells in the cancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ...

Ngày tải lên: 18/06/2014, 19:20

... All the patients underwent radical surgery, and none of them had undergone either chemotherapy or radiotherapy before the collection of the samples The histopathologic characteristics of the ... MCCstroma, the count of tryptase-positive mast cells in the cancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ... MCCstroma, the count of tryptase-positive mast cells in the cancer stroma of the primary colon tumor; MCCadjacent, the count of tryptase-positive mast cells in the normal mucosa adjacent to the colon cancer; ...

Ngày tải lên: 20/06/2014, 03:20

... Isolation, culture and cell surface epitope characterisation of IPFP cells The cells isolated from the IPFP proliferated in culture and reached confluence by day 14 The yield of cells by the end of passage ... CD105 (markers for mesenchymal stem cells) , and for CD29 (β1 integrin) The cells stained poorly for LNGFR and STRO-1 (markers on freshly isolated bone marrow stem cells) and sparsely for 3G5 (marker ... cytometry of the IPFP cell population showed a similar staining pattern to that of bone marrow-derived stem cells, and although they stained poorly for STRO-1 and for LNGFR, the expression of these...

Ngày tải lên: 09/08/2014, 10:20

... give a higher yield of stem cells and there is reduced pain and morbidity associated with the harvest of cells [8] In preliminary work, we identified perivascular cells in the IPFP tissue which ... [20] Materials and methods The defining properties of stem cells are self-renewal and multipotency Unfortunately, these crucial properties in adult stem cells show donor variability and may become ... (endothelial cell marker) stained endothelial cells in the tunica intima (Figure 1c,d) and anti-αSMA (smooth muscle cell marker) stained cells in the tunica media, forming the muscular wall of the...

Ngày tải lên: 09/08/2014, 10:23

... Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells compared with separate culture of AD-MSC and annulus cells alone Data from ... either cell type enhancing the overall ECM production, or by modification of the microenvironment of the 3D matrix through deposition of ECM components by either the AD-MSC or the annulus cells ... contributions HEG and ENH conceived the study and participated in its design and co-ordination HT and HEG wrote the manuscript HT, MK and RD performed all experiments and assays HT and RD retrieved...

Ngày tải lên: 09/08/2014, 10:23

... formation by the transformed P63+ basal /stem cells in the metastatic loci The data evidently demonstrated both cancer cell and stem cell characteristics of the transformed P63+ basal /stem cells, supporting ... formation and evaluate candidate therapeutic agents [23,24] In this study, we used the Hh overexpression mouse model to elucidate whether the PCSCs arise from P63+ basal /stem cells and to examine whether ... prostatic cancer stem cells [20] However, the tumorigenic activation of basal/ stem cells and their progression toward a metastatic status under the influence of Hh signalling remain to be further...

Ngày tải lên: 10/08/2014, 05:21

... AK1C3 [19] and CD44 [20] have been proposed to be the markers of cancer stem cells Their expression in tumor cells could correlate with their aggressive biological behavior, drug resistance and poor ... and clinical data to assess the precise function of these marker candidates and confirm them as real biomarkers, this proteomic analysis was effective to detect them and will be applied to other ... and SCLC, 93 in the overlap only between LCNEC and LCC, and 453 in the overlap among three groups; SCLC contained a total of 1,096 including 362 unique, 100 in the overlap only between SCLC and...

Ngày tải lên: 10/08/2014, 09:22

... Preface Cancer Stem Cells Theories and Practice does not “boldly go where no one has gone before!” Rather, Cancer Stem Cells Theories and Practice boldly goes where the cutting edge of research theory ... sharply focused as they ponder, “What causes the transformation from normal stem cells to cancer stem cells? ” The authors suggest that cancer stemloid (or stem cell-like cancer cells) ” might be ... to suspect they may be the source of these cells. ” What is more, “[c]ommon anticancer treatments such as radiation and chemotherapy not eradicate the majority of cancer stem cells. ” And making...

Ngày tải lên: 12/08/2014, 06:20

... normal stem cells to cancer stem cells Adult normal stem cells represent one purported origin of cancer stem cells What causes the transformation from normal stem cells to cancer stem cells? ... isolated cancer stem cells may be more accurately referred to as cancer stemloids (or stem cell-like cancer cells) (Blagosklonny, 2007) Although these cells may exist only as a minority within the cancer ... cancer stem cells Is the genome inherently unstable in stem cells? If the answer is yes, cancer stem cells may have two reasons for having an unstable genome: being a stem cell as well as a cancer...

Ngày tải lên: 12/08/2014, 06:20

... www.adultpdf.com 80 Cancer Stem Cells Theories and Practice Discovery of neural and glioma stem cells The discovery of adult neural stem cells paved the way for the glioma stem cell field Until the mid-20th ... neural stem cells and glioma stem cells, and may identify new CD133- glioma stem cells 4.4 New markers: Podoplanin and Integrin Alpha There are two new promising cancer stem cell markers The first, ... version www.adultpdf.com 90 Cancer Stem Cells Theories and Practice Fig CSCs and cancer therapy The CSC model helps us to understand why current cancer therapies fail and aids development of novel,...

Ngày tải lên: 12/08/2014, 06:20

... www.adultpdf.com Cancer Stem Cells in Lung Cancer: Distinct Differences between Small Cell and Non-Small Cell Lung Carcinomas 133 [90] Li, L., Neaves, W.B (2006) Normal stem cells and cancer stem cells: the ... demonstrated by Sutherland and colleagues (Inch et al., 1970; Sutherland This is trial version www.adultpdf.com 140 Cancer Stem Cells Theories and Practice et al., 1971) The floating cancer spheroids ... cancer stem cells Science, 317, 337 [19] Lewis, M.T (2008) Faith, heresy and the cancer stem cell hypothesis Future Oncol, 4, 585-589 [20] Yoo, M.H., Hatfield, D.L (2008) The cancer stem cell theory:...

Ngày tải lên: 12/08/2014, 06:20

... on their survival rate (Fan et al., 2004) Furthermore, the other novelty is the role of the miRNA 199b-5p in the impairing of the MB cancer stem cells by decreasing both SP cells and CD133+ cells ... self-renewal and decreased levels of apoptosis within cancer stem cells This results in further cancer progression (Modified from DeSano and Xu, "MicroRNA regulation of cancer stem cells and therapeutic ... this link between cancer stem cells and miRNAs Yet, the question remains – how does this link translate and occur within the cancer stem cells themselves? Most researchers believed and thus previous...

Ngày tải lên: 12/08/2014, 06:20

... different settings and tumor types In this chapter we will review this issue with depth Stem cells and cancer stem cell theory Stem Cells In order to understand cancer stem cells a brief description ... Cancer Stem Cells Cancer Stem Cells (CSCs) are tumor cells different from the rest of the tumor bulk in that they can drive the growth and spread of a tumor (Lobo et al 2007) They share their ... (2010) Metformin and cancer: Doses, mechanisms and the dandelion and hormetic phenomena Cell Cycle, in press This is trial version www.adultpdf.com 376 Cancer Stem Cells Theories and Practice McDermott,...

Ngày tải lên: 12/08/2014, 06:20