cancer initiating cells in colorectal cancer

... NHS National Institute for Clinical Excellence Guidance on Cancer Services Improving Outcomes in Colorectal Cancers Manual Update Improving Outcomes in Colorectal Cancers Cancer service guidance ... three main tasks The first was to breathe new life into dialogue about services for managing bowel cancer by highlighting key issues and enhancing and clarifying the original text In refocusing attention ... cancer deaths in the 34 35 36 37 38 20 Bingham SA, Day NE, Luben R, Ferrari P, et al Dietary fibre in food and protection against colorectal cancer in the European Prospective Investigation into...

Ngày tải lên: 28/03/2014, 23:20

... during CRC carcinogenesis, an effective cytotoxic effect induced by 5-FU in the KIAA0247expressing cells could be crucial in controlling the G2/ M checkpoint and in decreasing the number of cells ... Chloroquine potentiates the anticancer effect of 5-fluorouracil on colon cancer cells BMC Cancer 2010, 10:370 35 Cunningham D, Atkin W, Lenz HJ, Lynch HT, Minsky B, Nordlinger B, Starling N: Colorectal ... prostate carcinoma DU145 cells to doxorubicin-induced growth inhibition, G(2)-M arrest, and apoptosis Clin cancer res., 8: 3512-3519, 2002 Clin Cancer Res 2002, 8:3311-3314 Owa T, Yoshino H, Yoshimatsu...

Ngày tải lên: 18/06/2014, 19:20

... during CRC carcinogenesis, an effective cytotoxic effect induced by 5-FU in the KIAA0247expressing cells could be crucial in controlling the G2/ M checkpoint and in decreasing the number of cells ... Chloroquine potentiates the anticancer effect of 5-fluorouracil on colon cancer cells BMC Cancer 2010, 10:370 35 Cunningham D, Atkin W, Lenz HJ, Lynch HT, Minsky B, Nordlinger B, Starling N: Colorectal ... prostate carcinoma DU145 cells to doxorubicin-induced growth inhibition, G(2)-M arrest, and apoptosis Clin cancer res., 8: 3512-3519, 2002 Clin Cancer Res 2002, 8:3311-3314 Owa T, Yoshino H, Yoshimatsu...

Ngày tải lên: 20/06/2014, 03:20

... after culturing with any combination of drug in HCT116 cells, but were significantly increased in GEO cells treated with the combination of CDO and 5-FU (Fig 6A) In both HCT116 and GEO cells, there ... that CDO alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating the presence of senescence ... unchanged in HCT116 cells (B) Standard immunoblots for cyclin and CDK levels in GEO cells (C) Immunoprecipitation experiments highlighting cyclin/CDK interactions in the GEO cell line (i–ii)...

Ngày tải lên: 07/03/2014, 15:20

... declined in gastric cancer [35], we speculated that PTEN might participate in the VD3-induced apoptosis in gastric cancer cells To investigate this, undifferentiated HGC-27 adenocarcinoma cells ... application of vitamin D as a novel molecular target in gastric cancer therapies in association with the use of TSA ⁄ NaBu and 5-Aza Results Vitamin D induced apoptosis in gastric cancer cells through ... melanoma cells, including those in myeloid leukemia, and breast, prostate, colon, skin and brain cancer [4– 8] Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was originally discovered...

Ngày tải lên: 22/03/2014, 21:20

... These surprising findings suggest that TCF4 might be the main binding partner for b-catenin during development and progression of colorectal cancer whereas an enhancement of Wnt/bcatenin transcriptional ... TCF4 expression in colorectal cancer To investigate the localisation of LEF-1 and TCF4 in human colorectal cancer, we evaluated the expression of these proteins by immunostaining on tissue microarrays ... (A) nor in cells of the invasion front (B) 46% of cases showed TCF4 expression which could be found either only in main tumour areas (C) which occurred in 28 cases or only in cells of the invasive...

Ngày tải lên: 18/06/2014, 16:20

... expression in colorectal cancers, adenomas and adjacent normal colorectal mucosa Staining was (A) negative in normal colorectal epithelium cells, (B, C) weak to moderate in the nuclei of colorectal ... in colorectal cancer CK2a protein expression was analyzed in 144 patients (104 with CRC and 40 with colorectal adenoma) Staining for CK2a was nearly negative in all of the normal Page of 11 colorectal ... Murray T, Thun MJ: Cancer statistics, 2008 CA Cancer J Clin 2008, 58:71-96 Sung JJ, Lau JY, Goh KL, Leung WK: Increasing incidence of colorectal cancer in Asia: implications for screening Lancet Oncol...

Ngày tải lên: 18/06/2014, 19:20

... apoptosis in colorectal cancer cell lines [13,14] In this study we investigated whether doxycycline works in synergy with cisplatin and oxaliplatin or not in HT 29 colorectal cancer cell line Methods ... not reveal much increase in caspase activity levels in cells treated with cisplatin and oxaliplatin in combination with doxycycline (p = 0.001 and p = 0.039 respectively) These findings corresponded ... with the findings obtained in caspase gene expressions Discussion Colorectal cancer constitutes the third most common cancer and the second leading cause of cancer deaths in UK About one in 20 men...

Ngày tải lên: 09/08/2014, 04:20

... Apoptosis induction by isoproterenol or propranolol in combination with irradiation in BGC-823 and SGC-7901 cells There was an increasing rate of apoptosis in gastric cancer cell lines in response ... activity in cancer cells, thus making the cells radioresistant [12] Activation of NF-B by various stimuli, including inflammation, stress and radiation, involves degradation of the inhibitory ... preventing pancreatic carcinoma by aspirin Cancer 2005, 103:2485-2490 26 Takada Y, Kobayashi Y, Aggarwal BB: Evodiamine abolishes constitutive and inducible NF-κB activation by inhibiting InBa kinase...

Ngày tải lên: 09/08/2014, 09:20

... Germany) At least 30,000 cells (8.0mm2) were collected directly into a 1.5mL low-binding plastic tube Proteins were extracted and digested with trypsin using Liquid Tissue™ MS Protein Prep kits (Expression ... errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids as variable ... illustrated in a three-dimensional plot to judge whether a protein is specifically expressed in a given cancer group Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23...

Ngày tải lên: 10/08/2014, 09:22

... suppression in colorectal cancer patients Clin Cancer Res 2010, 16:4105-4112 Ohtani H: Focus on TILs: prognostic significance of tumor infiltrating lymphocytes in human colorectal cancer Cancer Immun ... Foxp3+ cells This indicates that the Treg population was not diminished in IBD patients, a finding in direct contrast to Clarke et al We are currently investigating this further to examine the role ... T cells in peripheral blood and tumour infiltrating lymphocytes in colorectal cancer patients Cancer Immun 2007, 7:7 Clarke SL, Betts GJ, Plant A, Wright KL, El-Shanawany TM, Harrop R, Torkington...

Ngày tải lên: 10/08/2014, 10:21

... transmembrane glycoproteins: the extracellular domain has the ligand binding site and also contains specific sequences that are involved in dimerization, while the intracellular domain is a catalytic ... detected in many human cancers, including CRC Monoclonal antibodies that target EGFR can be effective as anticancer therapy in several ways They can block ligand binding to the receptor, aborting ... predictors for response to cetuximab containing chemotherapy in colorectal cancer patients Identification of new KRAS mutation in codon12 ASCO 2008 Gastrointestinal Cancers Symposium 2008 Tejpar S, Peeters...

Ngày tải lên: 10/08/2014, 22:20

... level of 10 cells per ml of blood (Figure 1) In samples containing cell per ml of blood (10 cells total), marker expression was detected in 2/3 samples indicating some loss of cells during the immunobead ... markers of disseminated breast cancer cells Endocr Relat Cancer 2006, 13:1033-1067 Slade MJ, Coombes RC: The clinical significance of disseminated tumor cells in breast cancer Nat Clin Pract Oncol ... samples containing an estimated 100 or 1000 seeded cells were positive for all markers in 3/3 replicates In samples containing an estimated 10 seeded cells, TACSTD1 was detected in 2/3 replicates,...

Ngày tải lên: 10/08/2014, 22:20

... Angiogenesis in Gastric Cancer Cancer Microenviron 2009 in press 14 Waugh DJ, Wilson C: The interleukin-8 pathway in cancer Clin Cancer Res 2008, 14(21):6735-41 15 Nishimoto N: Interleukin-6 in rheumatoid ... factor induces protein kinase D-dependent production of proinflammatory cytokines in endothelial cells Am J Physiol Cell Physiol 2009, 296(4):C821-7 13 Kitadai Y: Cancer- Stromal Cell Interaction ... angiogenesis in gastric cancer [13], and acquisition of chemotherapeutic resistance in androgenindependent proliferation of prostate cancer cells [14] Finally, there is extensive experimental and clinical...

Ngày tải lên: 11/08/2014, 00:22

... of deviating skipping -3 -2 -1 Relative amounts of deviating inclusion skewed TIN (sTIN): Preferential exon skipping or inclusion overall TIN (oTIN): High or low total amounts of deviating exon ... deviating skipping 1.5 0.5 -2.5 -2 -1.5 -1 -0.5 0.5 Relative amounts of 1.5 deviating inclusion -0.5 -1 -1.5 -2 skewed TIN (sTIN): Preferential exon skipping or inclusion overall TIN (oTIN): High ... (Table S5A in Additional file 2) However, patients with either sTIN or oTIN cancers (a) Splicing Stage MSI-status sTIN oTIN (b) Stage II CRC / MSS tumors / no sTIN / no oTIN Splicing Stage MSI-status...

Ngày tải lên: 11/08/2014, 12:21

... peritoneal carcinomatosis from gastrointestinal cancer Cancer Invest 2005, 23(2):155-172 Sugarbaker PH: New responsibilities in the management of colorectal cancer with peritoneal seeding Cancer Invest ... DC+AK: dendritic cells and activated killer cells; ELISA: enzyme-linked immunosorbent assay; IFN-gamma: interferon gamma; IL2: interleukin 2; IL12: interleukin 12; TDL: tumor draining lymph nodes; ... Figure Cytological findings of ascites Cytological findings of ascites (a) Papanicolaou stain of ascites on February 2007 (before treatment) Carcinoma cells varying in size with prominent nucleoli...

Ngày tải lên: 11/08/2014, 19:21

... tetraploid intermediate Another study investigated both tetraploid cervical cells and HPV state in precancerous cervical cells, results showed that tetraploid cervical cells were elevated in women ... formation or tumorigenic ability in nude mice between normal HaCaT cells expressing all three oncogenic proteins and E5-induced bi-nucleated HaCaT cells expressing E6 and E7 In our hypothesis, cell fusion ... mechanisms by which HPV induces cervical cancer According to this hypothesis, blocking HPV E5-induced cell fusion is a promising way to prevent the progression of cervical cancer Additionally, establishment...

Ngày tải lên: 12/08/2014, 01:21

... 10% of all cancer incidence and mortality in 20132 Consistent with trends in developed countries, CRC is the most frequent cancer in Singapore, accounting for 14% to 18% of all cancer incidence ... 2005-20093 Colorectal cancers usually begin as non-cancerous polyps in the inner lining of the colon or rectum4 Over many years, adenomatous polyps in the dysplasic colon may develop into pre-neoplastic ... invasion (A-B) STMN1 overexpression in weakly invasive HCT116 cells significantly increased matrigel invasion (*** p

Ngày tải lên: 09/09/2015, 11:25

... and the role of this pathway in rapamycin sensitivity in cancer therapy; To examine the combination therapy by using PDK1 inhibitor and mTOR inhibitor in colorectal cancer The present study identifies ... signaling and oncogenic transformation, making PIK3CA a druggable target in cancer therapy (Liu et al., 2009) Many PI3K inhibitors have been developed in clinical setting, including pan-PI3K inhibitors, ... strategy in treating rapamycin-resistant colorectal cancer 32 Chapter II: Materials and Methods 33 2.1 Cell lines and cell culture 2.1.1 Colorectal cancer cell lines The human colorectal cancer...

Ngày tải lên: 09/09/2015, 18:52