callus and cell suspension cultures
Báo cáo y học: "In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures" pps
Ngày tải lên :
13/08/2014, 15:21
... Pharmaceutical (China) Cell lines and cell culture Malignant cell lines in this study include human lung adenocarcinoma epithelial cell line A549 (ATCC CCL-185), human breast adenocarcinoma cell line MCF-7 ... euchroma (Royle) Johnst cell suspension cultures exhibits specific in vivo and in vitro antitumor effects Abbreviations A549: human lung adenocarcinoma cell line A549; Bcl-2: B cell lymphoma/lewkmia-2; ... the cell lines of A549, Bel-7402, MCF-7 and LLC Acetylshikonin was diluted and added to target cells in triplicates with final concentrations at 25.6, 12.8, 6.4, 3.2, 1.6, 0.8, 0.4 μg/ml The cells...
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báo cáo khoa học: " UV-B-induced signaling events leading to enhanced-production of catharanthine in Catharanthus roseus cell suspension cultures" pps
Ngày tải lên :
12/08/2014, 05:20
... (a), MBPK activity (b), Tdc and Str gene expression (c) and accumulation of catharanthine (d) in cell suspension cultures of C roseus Six-day-old cell suspension cultures were pre-treated with ... (a), MBPK activity (b), Tdc and Str gene expression (c) and accumulation of catharanthine (d) in cell suspension cultures of C roseus Six-day-old cell suspension cultures were preincubated with ... inhibitor) and SB 600125 (JNK inhibitor) were a kind gift from Prof Anjali Karande, I.I.Sc, Bangalore Cell culture and treatments of cells with UV-B and chemicals C roseus suspension- cultured cells...
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Tài liệu Báo cáo " Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis Ha et Grushv " doc
Ngày tải lên :
12/02/2014, 10:20
... suspension culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of Ngoc Linh ginseng cell culture Materials and methods Induction of callus Fresh mountain ... on cell growth and ginsenoside production The effect of initial sucrose concentration (0, 20, 30, 50, 60 and 70 g/L) was also investigated in suspension cultures of P vietnamensis for biomass and ... concentration on cell growth and ginsenoside production The effect of the initial nitrogen concentration in the medium for cell growth and metabolite production was studied in P vietnamensis cell cultures...
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Báo cáo " Cell suspension culture Panax ginseng C. A. Meyer: Role of plant growth regulators and medium composition on biomass and ginsenoside production " docx
Ngày tải lên :
14/03/2014, 10:20
... saponin and polysaccharide by cell cultures of P quinquefolium Pro Biochem., 33: 671-675 Liu S and J.J Zhong 1997 Simultaneous production of ginseng saponin and polysaccharide by suspension cultures ... increase biomass and ginsenoside yield of ginseng cell culture Materials and methods Stock cell culture and culture condition Stock suspension cells of P ginseng were maintained in MS medium The ... C.O., S Liu, Q.S Li, J.T Yu and J.J Zhong 1999 Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng production by suspension cultures of P ginseng Pro...
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Báo cáo " Effects of macro elements on biomass and ginsenoside production in cell suspension culture of Ngoc Linh ginseng " docx
Ngày tải lên :
28/03/2014, 10:20
... 1.5, and 2.0) of MgSO4 were more favorable for both cell biomass growth and ginsenoside accumulation, as seen by the highest cell biomass DW (10.4 g) and ginsenoside content (5.5 mg/g DW) Cell ... metabolite biosynthesis In cell suspension cultures of P ginseng and P notoginseng, a low initial concentration of phosphate in the medium sufficiently promoted both cell growth and ginsenoside accumulation ... cell suspension cultures of Nicotiana tabacum Planta Med 42 (1981) 167 [12] S.H Mantell, H Smith., Cultural factors that influence secondary metabolite accumulation in plant cell and tissue cultures, ...
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Báo cáo y học: " The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures" potx
Ngày tải lên :
12/08/2014, 15:21
... lymphocytes and 5–20% monocytes The cells were resuspended in cell media with 1% FCS to million white blood cells/ml and plated in 48 well cell culture polystyrene clusters (Corning) and cultured ... Calu-3 cells Conditioned media and transwell studies PBMCs and lung epithelial cells were cultured alone in cell media with 1% FCS for 18 hours The cells were centrifuged at 200 g for minutes and ... secretion from PBMC and lung epithelial cell co -cultures (Table and Figure 6.) The IFN-γ (10 ng/ml) mediated IP-10 secretion in both A549/PBMC co -cultures and Calu-3/PBMC co -cultures was dose dependently...
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Senescence and Cell Cycle Control
Ngày tải lên :
25/10/2013, 21:20
... suppression and cell mortality Cell 85:27–37 Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW (1997) Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a Cell ... human fibroblasts by p16 and the polycomb protein Bmi-1 Mol Cell Biol 23:389–401 Itahana K, Campisi J, Dimri GP (2004) Mechanisms of cellular senescence in human and mouse cells Biogerontology 5:1–10 ... in cultured mouse cells p19ARF is predominantly upregulated during senescence by oncogene activation and/ or oxidative stress Senescence and Cell Cycle Control 265 In human cells undergoing replicative...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
Ngày tải lên :
19/02/2014, 05:20
... the daughter cells at the end of cell division and in cellular autolysis [9], where it mediates the release of toxins that damage the host tissues and allows the entry of pneumococcal cells into ... analysis of ligand binding to C-LytA (A) Conformational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM choline; ... ipratropium and 30 mM atropine Bars represent lm (B) Cell viabilities of the cultures at and h (black and gray shading, respectively) after the compounds (50 mM choline, 30 mM atropine, and 20 mM...
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Báo cáo khoa học: The dipeptidyl peptidase IV family in cancer and cell biology pot
Ngày tải lên :
06/03/2014, 09:22
... endothelial cells, pancreas, liver astrocytes Acts on activated T cells Acts on lymphocytes, dendritic cells, haematopoetic cells LD78b (CCL3 ⁄ L1) Eotaxin (CCL11) Expressed by T cells, B cells and monocytes ... recruitment of CD26 and CD45 to these rafts [80] and induces T -cell activation DPIV family in cancer and cell biology Fig Features of the effects of DP upon T -cell activation ⁄ function Cell- surface ... literature that DPIVand FAP-exerted effects on cell behaviour are cell- type dependent For example, while FAP overexpression in 293T cells was associated with decreased cell adhesion and cell migration,...
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Understanding the participatory news consumer - How internet and cell phone users have turned news into a social experience pdf
Ngày tải lên :
06/03/2014, 21:20
... incomes, and broadband and wireless users—report more frequent chance encounters with news as well as more frequent hunting and gathering experiences The youngest adults (those under age 30) and cell ... groups: women, African-Americans, and older Americans (those 65 and older) By comparison, those who are internet users and those who have a cell phone but no landline are less likely to get local ... the cell- only population (those who have dropped their landline and rely exclusively on their cell phone) get radio news on a typical day, compared with 39% of those who rely exclusively on landlines...
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Báo cáo khoa học: Linking pseudouridine synthases to growth, development and cell competition potx
Ngày tải lên :
06/03/2014, 22:21
... were chosen, and their total wing area, anterior (A) and P compartments area and cell density were measured (see Experimental procedures) Cell size was then estimated as the inverse of cell density ... from defects in cell adhesion and ⁄ or cell communication mfl silencing triggers apoptosis and sorting out of D cells towards the V compartment To assess the specific effects on cell apoptosis, ... cells expressing different levels of Mfl or still unexplained cell nonautonomous effects, such as defects in cell communication and ⁄ or cell affinity In order to evaluate the growth of mfl 05 cells...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... l-glutamine and 5% fetal bovine serum) and maintained at 37 C and 5% CO2 For translocation experiments, cells were removed with 0.05% trypsin EDTA, and plated with cell solution of ã 106 cellsặmL)1 ... prepared for cell assay and was added following the same procedure described for the time course cell assay The cells were washed with NaCl Pi and xed after 400 min, and the standard immunocytochemical ... regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report the results of cell- based, in vitro and in silico experiments...
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Báo cáo khoa học: Inhibition of urokinase receptor gene expression and cell invasion by anti-uPAR DNAzymes in osteosarcoma cells pot
Ngày tải lên :
16/03/2014, 22:20
... control and mutant DNAzyme-treated cells (Fig 5) Inhibition of Saos-2 cell invasion by Dz720 The plasminogen activator system can promote the breakdown of ECM and facilitate cell invasion and metastasis ... controls (P < 0.01) (Fig 6) Saos-2 cells treated with the active Dz720 had 42% and 17% fewer cells on the lower side of the filter compared with untreated control cells and cells treated with Dz720 mutant, ... uPA and uPAR to further inhibit cancer cell invasion and metastasis complex, the medium was replaced with DMEM containing 10% fetal bovine serum Cells were collected and assayed by northern and...
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Anatomy, Histology, and Cell Biology PreTestTM Self-Assessment and Review pptx
Ngày tải lên :
22/03/2014, 21:20
... with glands in the fundus and body that produce mucus (surface and neck cells), pepsinogen (chief cells), and acid and intrinsic factor (parietal cells) Intrinsic factor binds to vitamin B12 and ... (humoral immunity), T lymphocytes (cellular immunity), macrophages (phagocytic and one of several types of antigen-presenting cells), and mast cells Immunity and T and B cells There are two types of ... infection and release histaminases to counteract basophils and mast cells; and basophils, which contain histamine and heparin and assist mast cell function Bone marrow is the site of blood cell development...
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Targeting New Pathways and Cell Death in Breast Cancer Edited by Rebecca L. Aft docx
Ngày tải lên :
23/03/2014, 17:20
... glutamine, cysteine, and glycine It is the most abundant intracellular small molecule thiol present in mammalian cells and it serves as a potent intracellular antioxidant protecting cells from toxins ... tumor suppressors p53 and PTEN, and cell cycle inhibitors p21 Cip1/WAF1 and p27Kip1 (Park et al, 2008) Whereas the molecular machinery utilized by HER2 to promote cell migration and invasion remains ... Leu525 and Leu540, helix 12 and ligands, indicating, that these ligands most likely bind to ER’s conformation more closely related with the antagonist form X-ray crystallography of ER-4OHTAM and...
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Báo cáo khoa học: ERK and cell death: Mechanisms of ERK-induced cell death – apoptosis, autophagy and senescence doc
Ngày tải lên :
29/03/2014, 08:20
... Daniels MA (2009) ERK and cell death: ERK location and T cell selection FEBS J Martin P & Pognonec P (2009) ERK and cell death: cadmium toxicity, sustained ERK activation and cell death FEBS J Taylor ... ⁄ and MEK5 in those cells FEBS Journal 277 (2010) 2–21 ª 2009 The Authors Journal compilation ª 2009 FEBS 13 ERK and cell death S Cagnol and J.-C Chambard Fig The hallmarks of ERK-mediated cell ... of cell death that correlated with extensive cell rounding and the formation of cytoplasmic macrovacuoles, which pushed the nucleus and the cytoplasm to the side of the dying cell Although cell...
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Báo cáo khoa học: ERK and cell death: ERK1⁄2 in neuronal death Srinivasa Subramaniam1 and Klaus Unsicker2 pptx
Ngày tải lên :
29/03/2014, 08:20
... membrane damage and independently of caspase-3 J Cell Biol 165, 357–369 35 Mebratu Y & Tesfaigzi Y (2009) How ERK1 ⁄ activation controls cell proliferation and cell death: is subcellular localization ... hand, in non-neuronal cells, cytoplasmic retention of ERK1 ⁄ is required for death-associated protein kinase-mediated cell death [35] Thus, ERK1 ⁄ might promote cell death depending upon the cell ... multiple activators and targets, it is possible that activation and its cell death-promoting function may involve multiple partners and regulations [5] ERK1 ⁄ in neurodegeneration and ischemia ERK1...
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Báo cáo khoa học: ERK and cell death: ERK location and T cell selection pdf
Ngày tải lên :
29/03/2014, 08:20
... problem Interestingly, JNK activity and subcellular location are the same regardless of the ligand strength, ERK location and T cell selection whereas ERK activity and location change depending of ... a cell It was recently shown, that in thymocytes, positive and negative selecting ligands induce the localization of the components of the Ras ⁄ ERK cascade and active ERK into distinct subcellular ... negative selecting ligand Note the separate location of pJNK and pERK, and Ras-GRP1 ⁄ Grb2 ⁄ SOS ⁄ Ras ⁄ Raf at the plasma membrane lead to such distinct cell fates as survival and death Much is...
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Báo cáo khoa học: ERK and cell death: cadmium toxicity, sustained ERK activation and cell death pot
Ngày tải lên :
29/03/2014, 08:20
... J & Lenormand P (2009) Total ERK1 ⁄ activity regulates cell proliferation Cell Cycle 8, 705–711 ´ Lefloch R, Pouyssegur J & Lenormand P (2008) Single and combined silencing of ERK1 and ERK2 reveals ... cells [40] and in astrocytes This increase in turn leads to an increase in ROS and to mitochondrial impairment [41] Pretreatment with a calcium chelator reduced ROS production and increased cell ... inhibitor that is very effective in protecting cells from a caspase8-induced apoptosis [43], does not protect cells from cadmium toxicity, as cell phenotype and cell counts FEBS Journal 277 (2010) 39–46...
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