calcium signalling induced by cold or chilling stresses

... dithiothreitol), incubated with or without mm of the respective nucleotide or phosphate compound for h at °C, and stored for at least weeks at )80 °C before use for kinetic and molecular mass analyses ... tetrameric and dimeric forms in Figs and exhibited low or high catalytic efficiency Therefore, the various incubated hTK1 forms from Figs and were analyzed for their kinetic behavior with dThd, and ... form is more open than hTK1 Therefore, at present, it appears that the adenosine group is very loosely bound to hTK1 In the present work, the part of the phosphate donor that is necessary for...

Ngày tải lên: 18/02/2014, 12:20

... pCIneo-EGFP vector (EGFP vector) or the vector encoding httEx1-25Q-EGFP (25Q EGFP vector), httEx1-72Q-EGFP (72Q EGFP vector) or httEx1-103Q-EGFP (103Q EGFP vector) Cotransfections were performed using ... EGFP) or httEx1-103Q EGFP (103Q EGFP) Forty-eight hours after transfection, cells were washed with NaCl ⁄ Pi, and incubated with 2¢,7¢-dichlorofluorescein (DCFH-DA), and fluorescence was monitored by ... pCIneoEGFP vector (EGFP vector) or the vector encoding httEx172Q EGFP (72Q EGFP) In addition, cotransfections were performed using vectors encoding Hsp70 ⁄ Hdj-1, Hsp70 ⁄ Hdj-2, Hsp27 or mutant Hsp27(C137A)...

Ngày tải lên: 19/02/2014, 06:20

... micromanipulator and a 5246 transjector (Eppendorf) Immunofluorescence staining and confocal microscopy Cells were fixed in NaCl/Pi containing 3.7% paraformaldehyde for h, permeabilized for 30 with ... drastically reduced calcium binding activity D-BAPTA or the corresponding EGTA derivative could be used in calcium signalling experiments as controls for the known and unknown calcium independent ... thank Dr M Albrieux for help in calcium imaging, T Lorca for providing Xenopus extracts, peptide myosin light chain kinase and plasmid CaMKII, C Arnoult for advice and N Collomb for technical assistance...

Ngày tải lên: 19/02/2014, 16:20

... synthesis induced by VEGF165 and VEGF121 PF-4 does not affect VEGF121 -induced KDR phosphorylation We analysed the effects of PF-4 on the signalling pathways induced by VEGF165 and VEGF121 by investigating ... VEGF165 -induced phosphorylation of the KDR was inhibited by 45%, whereas VEGF121 -induced phosphorylation was unaffected (Fig 2A,B) Interestingly, the level of KDR phosphorylation induced by VEGF121 ... phosphorylation by 66% (Fig 3B) In contrast, the phosphory- Ó FEBS 2004 Inhibition of VEGF -induced ERK activation by PF-4 (Eur J Biochem 271) 3313 Fig Effect of PF-4 on KDR phosphorylation induced by...

Ngày tải lên: 19/02/2014, 16:20

... InsP3 or ryanodine receptors [14,21,24] Furthermore, NAADP-mediated Ca2+ release is neither potentiated by Ca2+ or Sr2+, nor inhibited by Mg2+ [14,21,29] Therefore in contrast to 4599 Calcium signalling ... of InsP3Rs and RyR antagonists or thapsigargin, but are abolished by agents that dissipate storage of by acidic organelles, such as the vacuolar H+ pump inhibitor, bafilomycin A1 This has been ... 2005 FEBS 4601 Calcium signalling by NAADP activated through both high and low-affinity sites on cholecystokinin A receptor This same study also demonstrated receptor specificity for the production...

Ngày tải lên: 20/02/2014, 01:20

... calcium after had little effect on the level of receptor- and calcium- induced Ins(1,4,5)P3, the removal of both calcium and CCh reduced the receptor- and calcium- induced Ins(1,4,5)P3 to 31% By ... to calcium or incapable of retaining it By contrast, once the calcium- dependent process has been activated by both receptor and calcium, the effects of calcium are retained, even after the calcium ... activated, by calcium The effect of calcium on Ins(1,4,5)P3 production was further investigated by separately analyzing receptor- and calcium- induced Ins(1,4,5)P3 increases The level of receptor-induced...

Ngày tải lên: 30/03/2014, 03:20

... harvested from the donor mouse and flushed with cold saline solution and stored in 4°C saline for either 20 or 60 before transfer into the recipient The recipient infrarenal abdominal aorta was isolated ... Cardiothoracic Surgery 2011, 6:2 http://www.cardiothoracicsurgery.org/content/6/1/2 Page of Figure Prolonged cold ischemia leads to earlier lesion formation Aortic allografts were exposed to 20 cold ... situation, with 20 in cold saline being the standard cold ischemic time in our laboratory and 60 in cold saline being considered our upper limit for immediate graft function Moreover, we were able...

Ngày tải lên: 10/08/2014, 09:23

... al.: Hypocellular scar formation or aberrant fibrosis induced by an intrastromal corneal ring: a case report Journal of Medical Case Reports 2011 5:398 Consent Written informed consent was obtained ... for publication of this case report and accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this journal Abbreviations Intacs: Intrastromal corneal ... after intracorneal ring segment implantation in keratoconic human corneas J Cataract Refract Surg 2007, 33:247-253 Fink AM, Gore C, Rosen ES: Corneal changes associated with intrastromal corneal ring...

Ngày tải lên: 10/08/2014, 23:20

... membrane regions (or rafts) are suggested in a recent report [42] which shows that signalling induced by receptors expressed in these regions differs from signalling induced by receptors expressed ... complex and thorough control of CCR5 signalling receptor activity, while bypassing clearance by D6 or DARC regulatory receptors (Table Page of 12 (page number not for citation purposes) Journal of ... chemokine receptor in model system to compare to regulatory receptors In order to investigate U83A activities on regulatory receptors and to compare them to those on signalling receptors, U83A binding...

Ngày tải lên: 11/08/2014, 08:22

... resisted the induced hypocalcaemia for more than hours The later decline in total calcium might be due to the excretion of EDTA bound calcium by the kidney Desmecht et al (1995) infused EDTA for 3-4 ... due to absorption of dietary calcium The absorbed calcium probably replaces EDTA-chelated fraction although this effect might be temporary The lack of correlation between pre infusion calcium concentrations ... comparison of methods involving monitoring of calcium homeostatic mechanisms Ionized calcium not total calcium monitoring may serve as a tool in monitoring the level of induced hypocalcaemic state in...

Ngày tải lên: 12/08/2014, 15:20

... supported by four legs Kneeling Body weight supported by front carpal joints and hind legs Dog-sitting Body weight supported by hindquarters and front legs Ventral lying, belly Body weight supported ... technician, for your excellent work in the field Also thank you Eva Norling for your help with the fieldwork Thanks to Boehringer Ingelheim Vetmedica for providing Metacam® The authors would also ... Body weight supported by belly Lateral lying, side Body weight supported by side Non-specific behaviour Walking/running Moving walking, trotting or galloping By udder Activity by the udder: suckling,...

Ngày tải lên: 12/08/2014, 18:22

... ice for 30 min, http://www.medsci.org Int J Med Sci 2011, 356 vortexed for 30 s after addition of 10% NP-40, then centrifuged at 5,000g for at 4℃ The pellet (nuclei) was resuspended in cold buffer ... protective in surgery- or trauma- related organ injuries occurring secondary to hepatic I/R Emulsified isoflurane reduced lung injury induced by hepatic I/R, as evidenced by amelioration of lung edema ... applicable to the clinical treatment of organ injury caused by hepatic surgery, transplantation or hemorrhagic shock Acknowledgements The study was supported by the National Natural Science Foundation...

Ngày tải lên: 25/10/2012, 11:00

... caused by VNR infusion, a 0.8-mg/mL solution of DEX was infused for 15 at mL/kg/hr just before (pre-treatment) or just after (post-treatment) the infusion of VNR In the control group, normal saline ... before and after VNR Tononi et al reported that post-treatment with DEX reduced phlebitis caused by VNR, so we tried to clarify the difference in the effect of DEX administration before VNR or ... with normal saline (Otsuka Normal Saline, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan) to provide a 0.6 mg/mL solution, while a 10 mg vial of DXR was dissolved and diluted with normal...

Ngày tải lên: 26/10/2012, 09:57

... TMAO before intact cells were assayed for TMAO:BV oxidoreductase activity (B) E coli strain RJ607 (/torA::hybO, DhybA) was transformed with a pUNIPROM vector expressing either torD (‘TorD’), or the ... 940 MÆcm)1 66 350 MÆcm)1 for FMalE-TorAhis Experimental procedures for TorDhis and Plasmids Overproduction of TorDhis was achieved from plasmid pQI-TorD [19], which incorporates a C-terminal hexahistidine ... authors and readers, this journal provides supporting information supplied by the authors Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or...

Ngày tải lên: 16/02/2014, 09:20

... supplied by the authors Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset Technical support issues arising from supporting information ... 0.5, or lM) for 96 h in the presence or absence of 10 lM GW9662 (G) Akt-1 in PGJ2 -induced 3T3-L1 adipocytes Cells were treated with PGJ2 at the indicated concentrations (0, 10 or 25 lM) for 96 ... 1B,C) Troglitazoneinduced adipocyte apoptosis could be detected by acridine orange (AO) ⁄ ethidium bromide (EB) staining or Hoechst 33258 staining (Fig 1B,C) Quantitative analysis by flow cytometry...

Ngày tải lên: 16/02/2014, 09:20

... e110 OsiS: oxidative stress -induced cysteine desulfurase 49 Miller JH (1972) Experiments in Molecular Genetics Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 50 Yoshida A & Freese ... RT-PCR experiments Gene Primers (5¢- to 3¢) rnpB Forward: Reverse: Forward: Reverse: Forward: Reverse: Forward: Reverse: Forward: Reverse: Forward: Reverse: isiA alr2495 alr3088 alr1457 Cysteine ... OsiS were used to record the respective baselines 10 Acknowledgements We are grateful to B Py for valuable discussions We thank the members of the Gudicci–Orticoni Laboratory for their help with...

Ngày tải lên: 18/02/2014, 04:20

... examined as a control for equal loading (lower) As a control for MKK4 and MKK7 activation, parallel cultures were treated with 0.5 M sorbitol for 30 or with 0.5 M sodium chloride for 15 (B) The cellular ... expression vector, and Y Inoue, Y Kaji and Y Hasegawa for technical assistance This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, ... Wakeling A, Korsmeyer SJ & Streuli CH (2002) Activation of BAD by therapeutic inhibition of epidermal growth factor receptor and transactivation by insulin-like growth factor receptor J Biol Chem...

Ngày tải lên: 18/02/2014, 13:20

... cells from apoptosis induced by the DNAdamaging agent ADR Pretreatment of the cells with 10 or 100 ngÆmL)1 EGF for 48 h markedly suppressed the cell death induced by 10 or 20 lm ADR, whereas ... TMK-1 cells against apoptosis induced by ADR (A) TMK-1 cells were pretreated or not with 1, 10 or 100 ngÆmL)1 EGF for 48 h Cells were then treated with 10 or 20 lM ADR for h and incubated in ADR-free ... factor, insulin-like growth factor, and fibroblast growth factor have been reported to transmit survival signals through the phosphorylation of Bad [34] Another target is caspase 9, phosphorylation...

Ngày tải lên: 19/02/2014, 06:20

... of the donor uorescence and acceptor absorption, and j2 is the orientation factor and accounts for relative orientation of the donor emission and acceptor absorption transition dipole Generally, ... corrected for sample dilution and the inner lter effect, introduced by cAMP and DNA at the excitation wavelength, according to the following formula [23] Fcor ẳ F 10PỵDAị=2 2ị where F and Fcor ... Dr S Garges for supplying us with the plasmid for production of CRP This work was supported by grant no P04A 031 16 from the State Committee for Scientic Research References Busby, S & Ebright,...

Ngày tải lên: 20/02/2014, 23:20

... protein kinase inhibitors on the enhancement by TS of LPS/IFN -induced NO production Protein kinases such as PKA and PKC, and various factors such as tumor necrosis factor receptor (TRAF6) and myeloid ... on LPS/IFN -induced NO production in VSMC Therefore, we used a concentration of 10 lM TS in this study, except in some cases Furthermore, TS enhanced the amount of iNOS protein induced by LPS/IFN ... is important for upregulation of PKC Recently, Neuzil et al reported the opposite findings to ours They proposed that TS -induced apoptosis in hepatopoietic and cancer cell lines is caused by the...

Ngày tải lên: 22/02/2014, 04:20