calcium phosphate cements in fracture repair

... action of the Golgi kinase and casein kinase on selected secreted phosphoproteins CSN1S1, aS1-casein; CSN1S2, aS2-casein; CSN2, b-casein; IBSP-II, integrin-binding sialophosphoprotein II; MEPE, matrix ... C-terminal sequence of OPN and the N-terminal sequence of dentin matrix acidic phosphoprotein (DMP1) [27,28] and long sequences of Glu residues in, for example, integrin-binding sialophosphoprotein ... supersaturated solution (A) In the absence of a competent sequestering peptide [i.e a in Eqn (5) is innite], ACP nuclei grow and mature into a crystalline or poorly crystalline calcium phosphate; under...

Ngày tải lên: 29/03/2014, 23:20

... stored in 10% neutral buffered formalin The material included in paraffin was prepared with a microtome, following the femoral long axis Sections were stained with hematoxylin and eosin, and ... cells during in vitro culture on calcium phosphate ceramics, showing that the cells populated the pores of the material Santos, in 2002 [22], developed a new cement based on alpha-tricalcium phosphate ... Congresso Internacional de São Paulo, São Paulo, RS, Brazil; 2005:20 virtual book, chapter XX Garg AK: Grafting materials in repair and restoration In Tissue Engineering: Applications in Maxillofacial...

Ngày tải lên: 11/08/2014, 20:20

... fracture healing in the group receiving an injection of 100 μg TP508 into the fracture gap This is in agreement with previous studies showing the benefit of TP508 in enhancing healing of various ... structural integrity of the fracture site The external fixators were removed by cutting through the pins using a diamond cutting disc and a haemostat to prevent pin spinning The pin remnants ... μg TP508 in 20 μl PBS into the fracture gap and Group IV as the control group received an injection of 20 μl PBS saline into the fracture gap Mechanical testing After termination, the skin over...

Ngày tải lên: 20/06/2014, 01:20

... knee flexion having been regained by months post-revision surgery However, in August 1998 he began developing some medial tibial pain and mild swelling in the right knee, which continued and by ... medial pain and swelling in the left knee A bone scan showed increased activity in the medial femoral condyle indicating possible localised osteolysis, and there was some low-grade uptake in the ... unable to find any data in the literature pertaining to the use of bone cements in the context of osteolytic cysts in knee arthroplasty, however these analogues have been successfully used in the...

Ngày tải lên: 20/06/2014, 04:20

... 545 nm The intensity of the colour is directly proportional to calcium concentration in the sample Inorganic phosphate was determined using the phosphomolybdate/UV method of Daly and Ertingshausen ... Biochem 1967, 18:521-531 Daly JA, Ertingshausen G: Direct method for determining inorganic phosphate in serum with the "CentrifiChem" Clin Chem 1972, 18:263-265 Mancini G, Carbonara AO, Heremans JF: ... attending the Obstetrics and Gynecology Clinics, São Lucas Hospital, in Porto Alegre city, South region of Brazil All of the subjects were healthy, functioning individuals attending the clinics...

Ngày tải lên: 11/08/2014, 23:22

Ngày tải lên: 05/10/2012, 08:21

... potentially involved in proteinprotein interactions [13] This method indicates that among other candidates, residues Lys128 and Arg197 may be involved in protein protein interactions These residues being ... directed against recombinant C reinhardtii CP12 (1 : 2000) or a rabbit antiserum directed against recombinant C reinhardtii GAPDH (1 : 5000) Antibody binding was revealed using alkaline phosphatase ... Prediction of proteinprotein interactions by docking methods Curr Opin Struct Biol 12, 2835 Gontero, B., Lebreton, S & Graciet, E (2001) Proteinprotein interactions in plant metabolism In Annual Plant...

Ngày tải lên: 19/02/2014, 16:20

... skeletal muscle troponin C J Biol Chem 263, 2371–2376 28 Ramos, C.H.I (1999) Mapping subdomains in the C-terminal region of troponin I involved in its binding to troponin C and thin filament J Biol ... & Reinach, F.C (1999) Regulatory properties of recombinant tropomyosins containing 5-hydroxytryptophan: Ca2+-binding to troponin results in a conformational change in a region of tropomyosin outside ... The interaction of the calcium binding troponin (troponin C) with bivalent cations and the inhibitory protein (troponin I) Biochem J 137, 145–154 Hartree, E.F (1972) Determination of protein:...

Ngày tải lên: 20/02/2014, 11:20

... ribozyme metal-binding site utilizes both nonbridging oxygen atoms of the A9 phosphate as well as nitrogen N7 of the subsequent guanosine unit G10.1 [38] We were interested in determining whether ... coordinated the metal ion, suggesting that the sulfur atoms in both the proRP and proSP positions hindered direct contact with metal ions Interestingly, this PS enzyme regained its activity in ... only a minor effect both in the presence of Mg2+ and in the presence of Mn2+, causing a 30–60% increase in krel, the effect of the double substitution P1 ⁄ P8 was strikingly different, depending...

Ngày tải lên: 07/03/2014, 10:20

... aluminium block engines, prior to refitting the cylinder head, check the cylinder liner protrusion as described in Part C of this Chapter Refitting 11.28 TU engine series gasket markings A Engine ... centre timing belt cover as described in Section TU engine in- car repair procedures 2A•7 Loosen the timing belt tensioner pulley retaining nut Rotate the pulley in a clockwise direction, using a ... contacting the pistons when refitting the cylinder head), or refitting the timing belt When the timing holes are aligned with the special holes in the cylinder head and the front of the cylinder...

Ngày tải lên: 07/03/2014, 11:20

... [51] Enzymes in boxes were subjected to metabolic engineering in this study Metabolites and enzymes in italics were measured in wild-type and engineered strains Enzymes involved in polyol formation ... by a three- to sevenfold increase in 6PG and a slight tendency to an increase in NADPH in the Gsd11 strain It is possible that PPP flux is increased in the Gnd20 strain and that a higher G6PDH ... strains were obtained by cotransformation of the uridine requiring strain [39] NW129 (cspA1 goxC17 pyrA6) with lg of the plasmid pGW635 containing the pyrA gene and 20 lg of a plasmid containing...

Ngày tải lên: 07/03/2014, 17:20

... al., 1998 fracture healing single linear elastic principal tensile strain and hydrostatic stress Loboa et al., 2001 oblique fracture healing single linear elastic principal tensile strain and hydrostatic ... rapid than in primary healing Stages of repair during secondary fracture healing The process of bone repair by secondary healing can be divided into three overlapping stages – the inflammatory, ... returned to the preinjury state There are two main types of fracture healing: Primary and secondary 2.2.1 Primary healing Primary fracture healing, also known as direct healing, involves intramembranous...

Ngày tải lên: 09/03/2014, 00:20

... peak intensities increased with increasing CayAs molar ratios for all slurries tested This apparent peak increase indicated that additional amounts of this precipitate formed with increasing CayAs ... Soluble As concentrations following the aging step are also summarized in Table and plotted in Fig After months of aging, soluble As concentrations decreased with increasing CayAs molar ratios for ... xxx–xxx 11 Table Mineral formations and TCLP As concentrations in lime–As–kaolinite slurries following month curing CayAs molar ratio Lime–NaAsO2 –kaolinite Phases Lime–Na2HAsO4•7H2O–kaolinite Phases...

Ngày tải lên: 15/03/2014, 23:48

... camshaft runs in bearing caps which are bolted to the top of the cylinder head The inlet and exhaust valves are each closed by coil springs, and operate in guides pressed XU engine in- car repair procedures ... remaining cylinder head cover retaining bolts, along with their sealing washers 12 Refer to the information given in Chapter on depressurising the fuel system Slacken the retaining clips, and ... hoses, inlet duct and throttle housing duct to the cover, tightening their retaining clips securely Reconnect the battery 37 Fit the rear fuel hose retaining clip, and securely tighten its retaining...

Ngày tải lên: 16/03/2014, 12:20

... on phosphate binding Although having very different oxygen-binding properties, both Hbs respond to an increase in water activity with an increase on oxygen afnity, indicating preferential binding ... also indicating preferential binding of water molecules to the R state The allosteric effectors signicantly affect water and O2 binding (Fig 2), and both chloride and 0.1 mM ATP induced an increase ... by linear regression around half saturation Hemoglobin and methemoglobin concentrations were estimated using the extinction coefcients for human Hb [11] Data obtained from samples containing...

Ngày tải lên: 23/03/2014, 13:20

... present not just in transcription factors, but in basically all families of proteins involved in maintaining genomic stability, including DNA repair proteins and cell cycle control proteins [18] This ... elucidated In the cytosol of cells, MT is present in excess over DNA repair proteins; however, owing to the coupling of zinc-binding structures (either in MT or in zinc finger proteins) by selenium ... XPA protein were investigated in the second model In vivo, XPA is absolutely required for incision during NER by co-ordinating the binding of ERCC1– XPF and presumably activating XPG [49] In vitro,...

Ngày tải lên: 30/03/2014, 15:20

... 0.5–0.8 lM) in EGTA, weaker binding was evident in the presence of mM calcium (Fig 5) In addition a change in the interface occurs during calcium binding to IIG6 site (Fig 6) The interaction ... C (2001) Binding of gelsolin domain to actin An actin interface distinct from that of gelsolin domain and from ADF/cofilin Eur J Biochem 268, 6165–6175 18 Lin, K.-M., Mejillano, M & Yin, H.L (2000) ... is in interaction with the C-terminal a-helix of G6 domain The sequence 159–193, including the second actin interface, appears also in interaction with G6 domain Therefore, we tested the interaction...

Ngày tải lên: 31/03/2014, 01:20

... receptors, leading to intracellular calcium increase Involvement of extracellular calcium entry in the extracellular calcium- induced increase in [Ca2+]i To examine whether the increased [Ca2+]i ... proteins detected in rat cardiomyocytes Extracellular calcium, gadolinium and spermine induced changes in intracellular calcium concentration Elevating [Ca2+]o from mM to > 1.5 mM evoked an increase ... gadolinium also induced a concentration-dependent increase in intracellular calcium (Fig 4D) With mM Ca2+ in the bath solution, spermine from 1–10 mM induced a time- and concentration-dependent increase...

Ngày tải lên: 31/03/2014, 01:20

... essential cysteines and/or other residues in zinc finger structures interfering in metal binding domain Taken together, the above mentioned mechanisms indicate that DNA repair, zinc homeostasis, ... for toxic metal compounds and are involved not only in DNA binding but also in protein-protein interactions Thus, there is an increasing evidence for zinc binding as structures very sensitive ... zinc finger structures in their DNA binding motifs Within these structures, zinc is complexed to four cysteines and/or histidines, folding different structural domains mediating DNA-protein as...

Ngày tải lên: 27/06/2014, 19:20

... might allow inflammation to be inhibited with minimal effects on certain homeostatic mechanisms, their role in bone metabolism and repair remains unclear Review of the evidence To determine the role ... role of COX-2 in bone repair, investigators have studied fracture healing in animal models of COX-2 inhibition or deletion Although several studies have been reported at scientific meetings, only ... manage pain induced by skeletal surgery or a fracture has raised concerns On the basis of animal studies, COX-2 inhibitors would seem to be contraindicated in patients undergoing bone repair However,...

Ngày tải lên: 09/08/2014, 01:21