... region showed intense nestin immunostaining At the recovery stage of EAE (day 21 PI), the nestin immunostaining pattern was similar to the pattern at the peak stage of EAE, with fewer nestin-positive ... were found to express nestin and/or vimentin in rats with EAE This finding is in part Embryonic Intermediate Filaments, Nestin and Vimentin, Expressioninthe Spinal Cords of Rats with Experimental ... Tae-kyun Shin, Yong-duk Lee and Ki-bum Sim nestin, vimentin and GFAP in EAE lesions inthe spinal cords of Lewis rats to evaluate the activation capacity of brain cells during autoimmune inflammation...
... determined as the slope b ofthe linear relationships between the log values of relative conductivity (RC; %) ofthe incubation solution and the duration of bark tissue incubation (h) during the investigation ... differences inthe slope b (Fig 2) The method employed to assess the frost hardiness ofthe bark tissue (determination of an index of injury) was suitable to reveal the periods of frost hardening (in ... determination of frost hardiness We hypothesize that complete defoliation of oaks inthe spring – especially when occurring in consecutive years – reduces the frost hardiness ofthe bark in the...
... regulators of gene expressionThe findings of this study implicate a role for miRNAs in schizophrenia, and lead us to the hypothesis that there is altered processing of miRNAs during the miRNA ... miRNAs; the others yield one Furthermore, miR-7-2, miR-9-2, miR-9-3, miR-30b, and miR-30d are intergenic, and the others are intronic in coding genes Using a combination of approaches, we found the ... background) inthe PFC tissueof ≥60% ofthe study subjects These included robust detection of miRNAs previously known to be expressed inthe brain (for example, let-7a to let-7i) as well as brain-specific...
... whether P63+ basal/stem cells were activated under the influence of Hedgehog overexpression, we examined the pCX-shh-IG-injected prostates during the progression of PIN toward CaP to gain further ... expressed inthe P63+ basal/stem cells ofthe pCX-shh-IG-injected prostates, being located inthe cell membrane of P63+ basal cells in regions of primary PIN lesions (Figure 3B; arrow in (b) indicated ... shown inthe upper pictures Hedgehog signalling members were expressed inthe PIN (F-J) and CaP (K-O) ofthe pCX-shh-IG-injected prostates, in contrast to the absence of evident signal inthe pCX-IG-injected...
... After each injection, the 31-gauge needle was maintained inthe spinal cord for an additional to reduce the possibility ofthe leakage ofthe injected fluid from the site The procedure of animal ... resolution inthe alkaline ranges of IPGs [25,26] As shown in Figure 1B, the poor focusing for the three protein spots in 2DE could gain inaccurate quantification of their spot intensity Third, the ... Based on the spatial and temporal levels of 40-kDa CSPGs inthe injured spinal cord, injection into the injured spinal cord with chABC at the different time points post SCI was performed The hindlimb...
... genes These findings strongly suggest that miRNAs play a most important role in driving tissue terminal differentiation and maintaining tissue identity rather than in determining or regulating tissue- specific ... for determining the timing oftissue differentiation during larva period of Drosophila development and maintaining thetissue identity during the adulthood It should be noted that the human microarray ... comparing the distribution ofthe rank number ofthe targets between different tissues, we could find the relative expression levels ofthe total targets of a miRNA in each tissue compared to other...
... spherulin 3a and NKA48 contain only a single domain fold The stability of these two proteins is highly dependent on calcium binding [25] The typical domain motives contain a ‘tyrosine corner’ inthe ... result of loss of induction at the transcriptional level, of loss of activation ofthe synthetase protein itself, or of derepression of enzyme(s) catalysing PMLA degradation An interesting interplay ... express a distinct fragmin-like actin-binding Cell type expressionof spherulin 3b and polymalatase protein that controls in vivo phosphorylation of actin by the actin-fragmin kinase Eur J Biochem...
... unique expression profile indicates the importance of EcR isoform expressionin TCCs and OLCs during larval-pupal metamorphosis, although the roles ofthe individual isoforms in these cells remain ... arrows in (D) and (E) indicate the EcR-A- and EcR-B1-producing cells inthe tritocerebral region ofthe pupal brain, respectively Red arrows indicate the EcR-Aor EcR-B1-producing cells inthe subesophagal ... reorganization ofthe brain 20E induces differentiation ofthe optic lobe and adult eye in Manduca [5,36], while EcR is expressed during the puparium stage inthe optic lobe of Drosophila [16] Inthe present...
... be flavinylated either inthe WTHA strain or inthe flx1D-HA strain These experiments also showed that the availability and attachment of flavin cofactors are not involved inthe regulation of Sdh1p ... 5¢-UTR of methionine synthetase mRNA [46] Sequence analysis ofthe 5¢-UTR of this mRNA also reveals the presence of two upstream ORFs involved in regulating the translational efficiency ofthe main ... for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 55 Laemmli UK (1970) Cleavage of structural proteins during the...
... variants ofthe murine fucokinase genes were expressed in COS-7 cells in frame with a 10-amino acid E2Tag present inthe pQM vector The molecular masses of fucokinase proteins were determined by ... amino acid sequence of mouse L-fucokinase The predicted amino acid sequence for the coding area ofthe long splice variant of fucokinase consists of 1090 amino acids Due to alternative splicing, ... human fucokinase cDNA is similar to the long splice variant of mouse fucokinase cDNA at the 3¢ splice region The first and third methionines inthe murine sequence, inthe upstream end ofthe CDS,...
... that inflammatory cells may stimulate theexpressionof OPN in macrophages and astrocytes, in either an autocrine or paracrine manner Since theexpressionof OPN paralleled the clinical course of ... expressed inthe white matter ofthe normal spinal cord in our previous study [13] and inthe present study In rats having spinal cord infiltrates of inflammatory cells during EAN, intense CD44 ... spinal roots These two molecules may be involved in cell migration into the spinal cord inthe early stages of EAN Further study ofthe functional role of OPN will be required to determine whether...
... results and those of Mondal et al [26] in terms ofthe number and the contents ofthe OXA-IR neurons, the increase-tendency inthe number ofthe OXAIR neurons inthe LHA ofthe fasting rats was consistent ... starvation for survival The increase of NPY expressioninthe ARC may be stimulated by the decrease of leptin in blood at 84 h of fasting, but not on the OXA Theexpressionof OXA and NPY may rise ... study is to investigate the effect ofthe high-fat diet on theexpressionof OXA and NPY inthe hypothalamus ofthe induced SD obese rats as well as the effect ofthe fasting on normal SD rats...
... rh 84 rof SBP ni esorcus %03 ni detcetorpoyrc neht dna C ta thginrevo evitaxif emas siht ni dexif-tsop erew seussiT desu erew ,2.7 Hp ,SBP M 1.0 ni edyhedlamrofarap %4 evitaxif yb dewollof ,)2.7Hp ... epotipe na sezingocer dna seidobitna lanolcylop deifirup ytiniffa si ydobitna yramirp sihT )ASU ,zurC atnaS( 001 : detulid ,nilubolgonummi AkrT-itna tibbar htiw C ta rh 84-42 rof detabucni erew ... ,rotceV( seidobitna tibbar itna yradnoces detalynitoib htiw C ta thginrevo detabucni dna ,)spets gniwollof eht fo hcae neewteb demrofrep saw erudecorp hsaw emas( TR ta hcae nim 01 ,SBP ni semit dehsaw...
... decreasing 3, 4-estradiol quinone or indirectly via the inactivation of E2 The importance ofthe CYP1A1/CYP1B1 ratio in- vivo is evident inthe increased presence of 4-OH-E2 in breast cancer tissue ... changes inthe estrogen receptor have been found inthe breasts of iodine deficient rats compared to normal euthyroid animals suggesting that the iodine pathway may augment the synthesis ofthe estrogen ... which iodine/iodide could enhance the efficacy of Tamoxifen therapy: 1) having an additive effect on estrogen inhibition and 2) inhibiting theexpressionof CCND1 thus preventing or slowing the development...
... describe staining of study cases The criteria for scoring staining intensity were listed in table 2: To calculate the IRS, we assigned the following points for staining distribution: 1, 1-25% of cells; ... Interpretation of immunostaining scoring We employed the planimetric measurement features by using the “PALM RoboSoftware 3.2” (PALM MicroLaser Systems) software to determine the immunostaining ... binding of integrins to their ligands or on integrin-mediated static cell adhesion to the extracellular matrix [35] Instead, they regulate post-ligand binding events, including intergrinmediated...
... mechanisms regulating overexpression of CXCR3/CXCL10 might help in defining the role of these molecules in synovial inflammatory responses, offering new insights into elements controlling the immune ... evaluated the role of CXCR3/CXCL10 interactions inthe regulation of T-cell migration into the joints of patients with JIA We have demonstrated the presence of IP-10/CXCL10 inthe synovial tissue ... were incubated with Tris-buffered saline containing isotype-matched antibodies instead ofthe primary antibody; they were invariably negative (data not shown) The intensity of antibody staining...
... aggrecan in this tissue (see below) Within these structures, there were significant deposits of BMP-2 and BMP-7, as visualized by indirect immunofluorescence staining The staining was concentrated in ... Because the extraction process does not enable determination ofthe original site ofexpression within the bursa tissue, we tested frozen sections of bursa tissue for their ability to induce AP ... causes ofthe chondroid transformation ofthe supraspinatus tendon because the expanded bursa tissue can act on the tendon and surrounding tissues Expansion ofthe bursa, however, does not explain...
... metastases are increasing in incidence, we detail the radiation methods used to treat these intracardiac metastases, including specific precautions taken for the pacemaker leads within the field of radiation ... are increasing in incidence, have various methods of spread and can affect any anatomic region ofthe heart There have been several reports of cardiac metastases inthethe literature [3,4] Intracardiac ... additional mm margin in all dimensions Inthe free-breathing CT scan, which was used for the final treatment planning, the lateral left ventricle and medial left ventricle (including the interventricular...
... consequence of insulin therapy in diabetes altered the binding parameters of Muscarinic M1, M3 receptors and gene expressionof α7nAchR, AchE, ChAT and GLUT3 inthe cerebellum and AchE activity inthe ... piperidine mustard; D + IIH: Insulin induced hypoglycemia in diabetes; C + IIH: Insulin induced hypoglycemia in Control Competing interests The authors declare that they have no competing interests ... excitatory and inhibitory control over central and peripheral tissues Inthe present study, enhanced muscarinic M1 and M3 receptor binding inthe cerebellum of insulin induced hypoglycemia in both diabetic...