cage illumina microarray and real time rt pcr

... 2008, 6:61 Part 2: IFNγ real time RT- PCR validation Specificity IFNγ real time RT- PCR specificity is defined as lack of response to irrelevant peptides and HIV negative control peptide and positive ... to TIL/1000 PBMC) (B) Expanded dose response: Graph in (A) is expanded to show details at the lower doses Figure IFNγ real time RT- PCR specificity IFNγ real time RT- PCR specificity TIL cells ... http://www.translational-medicine.com/content/6/1/61 Figure IFNγ real time RT- PCR standard curve (linearity) IFNγ real time RT- PCR standard curve (linearity) Linear response (IFNγ plasmid copy number...

Ngày tải lên: 18/06/2014, 15:20

... with automation to further improve the timeframe for genotyping a NoV infection Conclusion A real time RT- PCR assay and a RLBH assay were developed and utilised to identify and genotype the causative ... with three previously published RT- PCR assays [7-9] A Real- Time SYBR green RT- PCR assay was then developed based on the primer pairs to detect and quantify both GI and GII NoV in the Irish population ... of human NoV by Real- Time RT- PCR 56 samples were taken from two outbreaks of NoV in two Irish hospitals in 2005 and 2006 These samples were applied to the GII NoV real- time assay and 26 samples...

Ngày tải lên: 18/06/2014, 18:20

... with automation to further improve the timeframe for genotyping a NoV infection Conclusion A real time RT- PCR assay and a RLBH assay were developed and utilised to identify and genotype the causative ... with three previously published RT- PCR assays [7-9] A Real- Time SYBR green RT- PCR assay was then developed based on the primer pairs to detect and quantify both GI and GII NoV in the Irish population ... of human NoV by Real- Time RT- PCR 56 samples were taken from two outbreaks of NoV in two Irish hospitals in 2005 and 2006 These samples were applied to the GII NoV real- time assay and 26 samples...

Ngày tải lên: 20/06/2014, 01:20

... Primers P2FW-Standard-EGY, P2FW-Variant-EGY, P2RV-Standard-EGY and P2RV-Variant-EGY and probe PRO2-EGY were used for detection of both lineages via the ROX channel Real time RT- PCR optimization ... commercial poultry and backyard birds in Egypt in 2006- 2010 by multiplex H5 RT- qPCR and a generic H5specific RT- qPCR [20] Additional file 2: Analytical specificity of the multiplex RT- qPCR for Egyptian ... multiplex RT- qPCR for detection and differentiation of clade 2.2.1p proper (black triangles) and 2.2.1v variant HPAIV H5N1 strains (open triangles) from Egypt compared to standard H5 RT- qPCR [20]...

Ngày tải lên: 12/08/2014, 01:22

... negative control (Fig and Fig 3) Comparison of real- time qRT -PCR and conventional RT- PCR Next, the detection limit of the real- time qRT -PCR assay and the conventional RT- PCR assay were compared ... -70°C for further use Table 3: Diagnostic field samples tested positive by real- time qRT -PCR or conventional RT- PCR Tissue samples Number Conventional RT- PCR positive Real- time qRT -PCR positive ... Figure Standard ch-1a curve of the PRRSV real- time qRT -PCR assay A 10-fold serial dilutions ranging from 9.6 to 9.6 × 105 copies of PRRSV RNA were tested in the real- time qRT -PCR The standard formula...

Ngày tải lên: 12/08/2014, 04:20

... copies/μl) and were used in analytical sensitivity tests All experiments were done in duplicate Real- time RT- PCR Real- time RT- PCR was performed using iScript™ OneStep RT- PCR Kit Probes in Chromo real time ... Page of conventional RT- PCR, rRT -PCR has a smaller risk of cross-contamination, higher sensitivity and specificity, and shorter per sample laboratory turnaround time Several rRT -PCR assays for H5N1 ... into real- time PCR, enabling a more flexible primer and probe design and improving amplification efficiency [21-23] In this study, we describe the use and evaluation of an LNA TaqMan rRT -PCR for...

Ngày tải lên: 12/08/2014, 04:21

... number of real- time RT- PCR experiments focusing on grape berries has been published Based on microarray and real- time RT- PCR data, UBQ-L40 [14] and one paralog of EF1-α [2] were previously reported ... levels of an aquaporin and a sucrose transporter during grape development Results and discussion Microarray datasets can be a rich source of information for selecting real- time RT- PCR reference genes, ... designed and conducted the real- time RT- PCR experiments, conducted real- time data analyses, and assisted in drafting the manuscript JS was responsible for coordinating and carrying out grape tissue...

Ngày tải lên: 12/08/2014, 05:20

... (11) Real- time RT- PCR and plants The detection and quantification of foreign DNA present in plants is becoming increasingly important Plants are subject to a myriad of infections, and real- time RT- PCR ... application of real- time RT- PCR involves analysis of dynamic changes in expression of tumorassociated antigens and cytokine expression during immunization (11) 230 PCR Real- time RT- PCR can also ... theory behind real- time RT- PCR However, to give an idea of the 226 PCR more practical aspects of the technique and to become more familiar with data sets a typical real- time RT- PCR experiment...

Ngày tải lên: 25/10/2013, 22:20

... đường chuẩn cho phương pháp định lượng real time PCR chẩn đoán PRRSV Đoạn mồi phản ứng realtime PCR sử dụng EvaGreen Mồi F7 R7 cho phản ứng realtime PCR theo Martınez cs, 2007 Trình tự mồi vị trí ... lượng PRRSV kỹ thuật realtime sử dụng mẫu dò (probe) Trong đó, kỹ thuật realtime PCR sử dụng thuốc nhuộm kỹ thuật định lượng tránh vấn đề Do đó, nghiên cứu quy trình realtime RT- PCR với EvaGreen ... hiệu phản ứng real time RT- PCR định lƣợng PRRSV Để kiểm tra tính đặc hiệu phản ứng, thực phản ứng real time RT- PCR với mẫu dương tính PCV âm tính với PRRSV (được xác định phương pháp PCR) , mẫu dương...

Ngày tải lên: 25/03/2014, 04:21

... fo stimil eht rof hcraes ruo ni lortnoc evitisop a sa desu saw gurd hcae fo noitulos lm/gµ 05 a dna lortnoc evitagen eht sa desu saw retaw dellitsiD selpmas lortnoC la te idnatihS olakanA 871 ... ,lm/gµ naht retaerg snoitartnecnoc ta tceted ylno dluoc ti hcihw ,revewoh ,muilorpma ot ytivitisnes fo kcal a detartsnomed msinagro siht ,yduts ruo nI lm/gµ naht rewllams snoitartnecnoc ta enodilozaruf ... deviecer noitartnecnoc gurd hcae fo etacilper a gniniatnoc ebut hcae taht hcus sebut eht ot dedda saw tnatanrepus ehT noitulos fo tuo llaf ot sirbed eussit wolla ot nim rof degufirtnec erew selpmas...

Ngày tải lên: 07/08/2014, 18:21

... development and subsequently as daily run control References RNA extraction with NucliSens EasyMAG extractor and real- time RT- PCR analysis on LightCycler 480 showed a higher sensitivity and precision and ... single-tube real- time reverse transcription -PCR assay Journal of Clinical Microbiology 2001, 39:4093-4096 Verstrepen WA, Bruynseels P, Mertens AH: Evaluation of a rapid real- time RT- PCR assay for ... Control was 1.64% and 0.82% for primer-probe set and respectively Table 2: Mean Ct/Cp values and %CV values for the undiluted OptiQual Enterovirus Run Control in different real- time RT- PCR settings...

Ngày tải lên: 12/08/2014, 04:21

... development and subsequently as daily run control References RNA extraction with NucliSens EasyMAG extractor and real- time RT- PCR analysis on LightCycler 480 showed a higher sensitivity and precision and ... single-tube real- time reverse transcription -PCR assay Journal of Clinical Microbiology 2001, 39:4093-4096 Verstrepen WA, Bruynseels P, Mertens AH: Evaluation of a rapid real- time RT- PCR assay for ... Control was 1.64% and 0.82% for primer-probe set and respectively Table 2: Mean Ct/Cp values and %CV values for the undiluted OptiQual Enterovirus Run Control in different real- time RT- PCR settings...

Ngày tải lên: 12/08/2014, 04:22

... biển gene cytokine: - Phương pháp RT- PCR real- time RT- PCR: phương pháp phản ánh mức độ biểu gene mã hóa cho cytokine mức phiên mã Những năm gần real- time RT- PCR ngày sử dụng nhiều để định lượng ... cs, 2007) Trong đề tài này, sử dụng kỹ thuật real- time RT- PCR để phát thay đổi mức độ biểu gene cytokine quan trọng hoạt động miễn dịch Vì real- time RT- PCR sử dụng rộng rãi để định lượng cytokine ... GraphPad Prism 29 3.2 Áp dụng quy trình real- time RT- PCR với số chất thử nghiệm Đầu tiên, kiểm tra khả phát biểu gene IL-2 TNF-α quy trình real- time RT- PCR vừa xây dựng với hai chất làm chứng...

Ngày tải lên: 07/02/2015, 15:05

... nhân gây BTCM EV71 kỹ thuật Realtime RT- PCR Định chuẩn xét nghiệm Real- time RT- PCR Qui trình chẩn đoán EV71 ba bước gồm phản ứng RT- PCR kết hợp với phản ứng Real- time PCR nghiên cứu chứng minh ... Phản ứng Real- time RT- PCR xem xác có tương đồng với kết phản ứng RT- PCR định tính EV71 Phản ứng Real- time RT- PCR sử dụng đoạn mồi EV có độ xác tốt bắt buột phải dương tính phản ứng RT- PCR mẫu ... phát triển kỹ thuật Real- time RT- PCR rút ngắn thời gian trả kết nhanh so với RT- PCR cổ điển (2 so với 10 giờ) Các nghiên cứu Đài loan Singapore [12], [17] cho thấy Real- time RT- PCR có độ nhạy cảm...

Ngày tải lên: 07/02/2015, 23:32

... Paisley, UK) and incubated at 37°C for hour Two μl of cDNA were used for each PCR amplification by "real time" technology (7300 Real Time PCR system, Applied Biosystems, Rotkreuz, Switzerland) according ... http://www.translational-medicine.com/content/6/1/58 Authors' contributions ES-T and NR performed qRT -PCR assays in WB, DMF designed the study and participated in the performance of qRT -PCR assays and in writing the paper DM helped collecting ... vaccinated donors and performed serological studies CF-M helped designing the qRT -PCR strategy GCS provided funding and helped designing the study and writing the paper PZ designed the qRT -PCR strategy,...

Ngày tải lên: 18/06/2014, 15:20

... by real- time PCR of Page of which two were positive by pp65 antigenemia assay and 12 were negative by both real- time PCR and pp65 antigenemia assay One donor was positive only by realtime PCR ... antigenemia and real - time PCR The highest copy number detected by real - time PCR was 29, 768 copies/ml and lowest was copies/ml The highest positive pp65 cell count recorded among them was 78 cells and ... immediately for pp65, and real time PCR for HCMV (qRT -PCR) The study was approved by both the institute’s research and ethics committee and informed consent was obtained from the donors and the renal...

Ngày tải lên: 12/08/2014, 02:20

... (miR159a and miR159b) have been sequenced more than 5,000 times in mature pollen Since the expression of these two miRNAs was not detected by either real- time PCR or regular RT- PCR (Table and Figure ... inflorescence and mature pollen by RT- PCR Expression of 27 miRNAs in inflorescence and mature pollen by RT- PCR The adaptor sequence is 46 bp long Depending on the length of the miRNA and the number ... our samples by using real time- PCR based on TaqMan MicroRNA assay (Applied Biosystems, Foster City, CA) TaqMan miRNA assay is based on stem-loop RT- PCR detection method, and the TaqMan probe...

Ngày tải lên: 12/08/2014, 03:20

... cells The real- time PCR (RT- PCR) technology utilizes the standard PCR method with the addition of measuring the accumulation of amplified DNA in real- time by a fluorescent signal RT- PCR uses the ... viruses and RT- PCR give results that are comparable with labeled and purified viruses, but with less effort and expense Altogether, these results clearly indicate the capacity of the RT- PCR technology ... we demonstrate that RT- PCR is an alternative, rapid and efficient method to study viral interaction with the cell surface RTPCR can complement or replace the expensive and timeconsuming methods...

Ngày tải lên: 12/08/2014, 04:21

... 26.66 28.80 28.95 Volume 8, Issue 2, Article R19 comment Sample Standard1 Standard1 Standard2 Standard2 Standard3 Standard3 Standard4 Standard4 Standard5 Standard5 Genome Biology 2007, R19.4 Genome ... quantification in real- time RT- PCR Nucleic Acids Res 2001, 29:e45 Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real- time quantitative RT- PCR ... exchange of qPCR data under the form of XML files [15] Data can be imported from a number of data formats Two standards (qBase internal format and RDML (Real- time PCR Data Markup Language)) and a number...

Ngày tải lên: 14/08/2014, 17:22