ca2 decreased on the cacc activity
Báo cáo y học: "Abnormal spatial diffusion of Ca2+ in F508del-CFTR airway epithelial cells" pptx
Ngày tải lên :
12/08/2014, 14:20
... cells) Then, the Ca2+ response produced by the NP-EGTA photolysis was the consequence of the presence and activity of IP3Rs This explains that the Ca2+ increase observed is only measured during the ... stimulated the activity of CaCC by the Ca2+ ionophore A23187 No variation was detected following the treatment of cells by miglustat (Fig 9) Then we examined the activity of CaCC stimulated by ER Ca2+ ... 10 μm the cell (Fig 8B) Thus treatment of CF cells with the corrector miglustat induces an ER spreading throughout the cells What is the consequence of the ER Ca2+ decreased on the CaCC activity? ...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
Ngày tải lên :
19/02/2014, 12:20
... effects on the activity of RTA Whereas, one had no effect on the activity of the enzyme (R258A), others reduced activity by one (R213A, R258D, R48A) or by two (R213D) orders of Ó FEBS 2003 Mutations ... In the second model (Fig 1B) the structure of the hexanucelotide is, on the whole, unchanged and the majority of the interactions that were seen in the first model are maintained However, the ... substitutions at either Asp75 or Asn78, both of which are highly conserved in the RIP family and were proposed to make interactions with the second G4 in the GAGA tetraloop in the second of the models...
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Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt
Ngày tải lên :
21/02/2014, 03:20
... ligands on the iron atom, and the only one that enters the active site bind on the less hindered face of the porphyrin which bears only one ortho-COOH substituent (Fig 7C) The inhibition of the peroxidase ... face of the porphyrin and, in the more favorable conformation, H2O2 binds to the iron on the same face of the porphyrin as the catalytic COOH residue, the imidazole binding to the iron on the opposite ... to the iron(III) of Fe(ToCPP) and Fe(ToCPP))13G10 The second part of our results concerns the binding of imidazole derivatives on the iron of Fe(ToCPP) either alone in solution or inside the...
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báo cáo hóa học: " The effect of hip abduction on the EMG activity of vastus medialis obliquus, vastus lateralis longus and vastus lateralis obliquus in healthy subjects" pot
Ngày tải lên :
19/06/2014, 10:20
... diminished EMG activity of the VMO in relation to that of the VL [7-9], and the delayed onset of VMO activation in relation to the VL [1012] caused by the inhibition of pain, effusion and atrophies[13] ... flexion compared with the higher activity of the VLL at 15° of flexion, demonstrating that the VMO and VLO muscle have the same behavior suggesting a synchronic antagonist stability role of the ... be related to the non collection of gluteus medius EMG activity during the three exercises tested Normalization methods using other types of muscle contractions or other angles of the knee joint...
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Investigations on the antiplasmodial activity of ferrocenyl chalcones
Ngày tải lên :
16/09/2015, 08:31
... zone existed where there was an interplay between these two proposed contributions of the metallocene The extent of each contribution may depend on the biological activity being considered The ... of the larger dihedral angle between the protons The coupling constants of the vicinal protons indicated that the ferrocenyl chalcones were obtained in the E configuration When the double bond ... Characterization by proton NMR spectroscopy The α,β-unsaturated linkage in the ferrocenyl chalcone gave rise to two doublets (δ – ppm) due to the vicinal protons on the double bond The trans (E) configuration...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 3
Ngày tải lên :
17/09/2015, 17:18
... ANTIMALARIAL ACTIVITY OF CHALCONES 6.1 Introduction The mode of action of chalcones in malaria remains uncertain As described in the Introduction (Section 1.3.1 ) the antimalarial activity of chalcones ... Effects on falcipain-2 and associated changes in the food vacuole on incubation The same chalcones that were tested for activity on the crude plasmodial extract were investigated for their effects on ... from the following evidences Firstly, inhibition of the enzymatic activity of the plasmodial extract was observed at a relatively high concentration (100 µM) of the chalcone, and even then, the...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 2
Ngày tải lên :
17/09/2015, 17:18
... drug On the other hand, there might be reasons to focus on only one activity to the exclusion of the other Regardless of the objective, defining structure -activity relationships for each activity ... that the alignment of the chalcones would be based on the α,β-unsaturated carbonyl linkage as it is the most consistent feature in all the chalcones In the case of antimalarial activity, the most ... chalcones, the energy-minimized conformations of the chalcones were aligned using the geometry of the α,β-unsaturated carbonyl linkage of the most potent member for antimalarial activity (27) In the...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 1
Ngày tải lên :
17/09/2015, 17:18
... bond (J Hα-Hβ =11-18 Hz) than the cis double bond (J Hα-Hβ = 5-10 Hz) By monitoring the coupling constants of the protons on the double bond in the 1H-NMR, it was concluded that all the chalcones ... electronic factors 3.3.2 Characterization of chalcones The successful synthesis of the targeted chalcones was confirmed by monitoring the position of the α,β-unsaturated carbonyl linkage in the ... 3.1 Introduction In this section, the rationale behind the design of the chalcones is presented The synthetic methods employed in the preparation of the chalcones are described and the evidence...
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Tài liệu The Influence of Human Activity on the Environment ppt
Ngày tải lên :
26/01/2014, 10:20
... stations produces carbon dioxide, sulphur dioxide and other greenhouse gases • carbon dioxide traps heat in the atmosphere and causes the temperature of the earth to rise • This leads to disruption ... growing population This causes several problems Burning the timber increases the level of carbon dioxide in the air Less trees means less carbon dioxide absorbed for photosynthesis Soil is eroded ... crops • They also kill harmless insects or can get washed into rivers and pollute the water • They may even end up in the food chain In the 1960s, DDT in the food chain threatened bird populations...
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Báo cáo khoa học: Effect of annealing time of an ice crystal on the activity of type III antifreeze protein pdf
Ngày tải lên :
07/03/2014, 05:20
... that the TH value reflects the fraction of ice-bound AFPs on the flat ice plane Indeed, TH increases in proportion with the total concentration of nfeAFP8, as can be seen in Fig 3A The fraction of ... type III AFP onto the flat ice plane (B) Overgrowth of the convex ice front, giving a low TH value (C) Possible secondary ice binding of type III AFP onto the convex ice front during the annealing ... stabilizes the convex ice front (Fig 5D), leading to the time-dependent change in TH activity This concept further explains the increased level of time-dependent amplification of TH at the lower...
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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
Ngày tải lên :
22/03/2014, 16:20
... efavirenz on the wild-type RT RDDP activity using the YonetaniTheorell plot [25] The results showed that when the HIV-1 RT RDDP activity was measured in the presence of increasing concentrations of one ... inhibition potency on the polymerase function; and (c) the K-61 analogue showed a fourfold reduction in its RDDP activity inhibition potency By contrast, when the AQ derivatives were tested on the ... al [30] the NNRTI binding pocket most closely resembles the RT unliganded conformation Therefore, the AQ derivatives were docked into the K103N RT in this conformation Under these conditions, we...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
Ngày tải lên :
23/03/2014, 04:21
... considered to be 100% in the control reaction mixture lacking heliquinomycin, and the proportions in the mixtures containing heliquinomycin were calculated in relation to the control value The ... solution (0.4 lL) containing or lacking heliquinomycin was added to the reaction mixture The final concentrations of heliquinomycin added to the reaction mixture are indicated at the top The DNA ... was synthesized only in the presence of the above complex The synthesis of the RNA primer was inhibited in the presence of heliquinomycin at an IC50 value of 25 lm The DNA polymerization activity...
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Báo cáo khoa học: Effect of magnesium ions on the activity of the cytosolic NADH/cytochrome c electron transport system pptx
Ngày tải lên :
23/03/2014, 06:20
... added after the mitochondria It also provides direct evidence that, in these latter conditions, the addition of magnesium mimics the results obtained with the addition of cyto-c, the only difference ... after the mitochondria (Fig 4, trace e) The data reported in Fig 4B are consistent with and similar to those in Fig on the oxidation of exogenous NADH as the expression of the activity of the complete ... present in the hypotonic medium before the addition of mitochondria (Mg-m), the release of cyto-c was significantly lower and amounted to 21% (the additional release was only 7%) The decrease in the...
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Báo cáo khoa học: The ‘pair of sugar tongs’ site on the non-catalytic domain C of barley a-amylase participates in substrate binding and activity potx
Ngày tải lên :
23/03/2014, 07:20
... oligosaccharide at the sugar tongs in the structure [3] In the present work, the sugar tongs site was demonstrated by site-directed mutagenesis of Tyr380 to be involved in enzymatic activity and conrmed ... Remarkably, comparison of the sugar tongs region indicated no conformational differences between AMY1 wild-type, S378P and Y380A acarbose complexes The only obvious difference was found for the Ala211-Pro218 ... to the active site cleft and the sugar tongs [21] The three rings dened at the Trp278Trp279 site corresponded to acarbose with the reducing-end glucose cleaved off and with the same orientation,...
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Báo cáo khoa học: The antibiotic activity of cationic linear amphipathic peptides: lessons from the action of leucine/lysine copolymers on bacteria of the class Mollicutes doc
Ngày tải lên :
23/03/2014, 17:21
... interestingly, dansylation increased its activity whilst the same modification decreased the activity of the 15-residue peptide Shorter peptides (8 or residues) displaying a b conformation in the membrane-bound ... spreading on agar plates cells treated for h with different peptide concentrations The minimal lethal concentration was defined as the lowest peptide concentration capable of killing 99% of the cells ... orientation should thus be considered the Ó FEBS 2003 most stable one for LK peptides, even if other orientations are possible (see below) As suggested by the ÔcarpetÕ model, aggregation on the membrane...
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Báo cáo khoa học: On the mechanisms of bananin activity against severe acute respiratory syndrome coronavirus pptx
Ngày tải lên :
28/03/2014, 23:20
... interface in the case of the former helicase Furthermore, the absence of the S259 OH group in the mutated viral enzyme prevents the formation of one hydrogen bond with bananin, accounting for the reduced ... accession number AY304495) on fetal rhesus monkey kidney cell line FRhK-4 in the presence of high concentrations of this adamantine derivative The half maximal effective concentration of bananin on ... deeper insight into the function ⁄ structure correlations for native and mutated forms of the viral enzyme Preliminary analysis on the frequency of amino acid substitutions among the 78 complete...
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Báo cáo khoa học: The two IQ-motifs and Ca2+/calmodulin regulate the rat myosin 1d ATPase activity pptx
Ngày tải lên :
30/03/2014, 16:20
... the concentration of free Ca2+ Next, we determined the ATPase activities for the different Myo1d constructs as a function of the free Regulation of Myo1d ATPase activity Ca2+ concentration (0.001158 ... deletion of the two C-terminal amino acids of the converter domain in the construct Myo1dDLV-IQ2 lead to a pronounced inhibition of the actin-activated ATPase activity while the basal ATPase activity ... inhibited the ATPase activity in a Ca2+ -independent manner to a similar extent as observed with Ca2+ for the other constructs containing either one or two IQ-motifs Ca2+ -independent and Ca2+ -dependent...
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Báo cáo khoa học: Effects of a tryptophanyl substitution on the structure and antimicrobial activity of C-terminally truncated gaegurin 4 doc
Ngày tải lên :
31/03/2014, 09:20
... helical conformation in the C-terminal part The disordered conformation in the C-terminal region of D24)37 GGN4 is probably due to the break of the peptide bond at position 23 In contrast, the finally ... tryptophanyl substitution at position 16 on the biological activity was confirmed for the native GGN4 Consistent with the results of the C-terminally truncated GGN4, the D16W substitution in the native GGN4 ... truncation In line with the CD results, the solution structures clearly support the idea that the D16W substitution of D24)37 GGN4 contributed to the restoration of the antimicrobial activity, ...
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Báo cáo Y học: Effect of coenzymes and thyroid hormones on the dual activities of Xenopus cytosolic thyroid-hormone-binding protein (xCTBP) with aldehyde dehydrogenase activity potx
Ngày tải lên :
31/03/2014, 21:21
... Kinetics of the inhibition of xCTBP/xALDH1 by T3 when NAD+ concentration was varied within the reaction mixture The reaction was performed at 24 °C with lg of xCTBP/xALDH1 The concentration of retinal ... NAD+ concentration within the reaction mixture The Km value, lM, was independent of the concentration of T3, but the Vmax value decreased from 0.18 to 0.08 lmolÆ min)1Æmg)1 with increasing concentrations ... xALDH1, were in the 10-7)10-4 M range These are high concentrations, even if the local distribution or accumulation of intracellular thyroid hormones was considered The present studies demonstrate that...
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