... P jacquemontii lectin is sufficiently evident from its inhibition and hemagglutinatin study The lectin is unique from that of other sialic acidspeci c lectins O-Acetyl sialic acid-speci c lectin ... O-acetyl sialic acid The HAI results when summarized suggest that the crab lectin was sialic acid-speci c with a high affinity for O-acetylated NeuAc Discussion The presence of naturally occurring ... fractions collected on ice in polypropylene tubes containing 100 lL of 100 mM CaCl2 at a rate of 0.3 mLÆmin)1 The presence of calcium chloride was required in the collected fractions because the...
... ‘nested PCR’, using the first PCR product as template To make use of the BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product of the nested PCR was replaced with the corresponding ... Technology, for the synthesis of TFM and constructive discussions This work was supported in part by Grants-in-Aid for Scienti c Research fromthe Ministry of Education, Culture, Sports, Science ... excessive amounts of bacteria or host cells This can be interpreted as follows to explain the regulatory mechanism of the intracellular Met concentration Under physiological Met or Cys concentrations,...
... acid sequence of novel COX is 97% identical with the COX sequence fromthe same coral species collected in the Florida Keys forming 15R-configuration products (Fig 1) Similar to the 15R-speci c ... (Eur J Biochem 271) characterize the COX enzyme fromthe S-variety of P homomalla, compare its primary structure and catalytic properties with its 15R-speci c counterpart, and locate the residues ... products formed from [1 4C] arachidonic acid with wild-type and mutant P homomalla 15SCOX expressed in Sf9 cells Incubations of [1-1 4C] arachidonic acid with recombinant coral COX proteins were carried...
... at the branching point Elucidation of the structure of the O-speci c polysaccharide by NMR spectroscopy The 1 3C NMR spectra of OPS-I and OPS-II were essentially identical, and therefore only the ... polysaccharide was studied further The 1 3C NMR spectrum (Fig 2) contained signals for four anomeric carbons in the region d 94.8–99.8, three CH3 -C groups (C6 of Rha and Fuc), one C- CH2 -C group ... Hydrolysis conditions: A, 2-M CF3CO2H, 120 C, h; B, 10-M HCl, 80 C, 0.5 h; C, 0.5-M CF3CO2H, 100 C, h DA, O-deacetylated; OPS, O-speci c polysaccharide; SD, Smith-degraded GLC detector response...
... Leu Ile Leu a Calculated using the program DSSP b Calculated according to Facchiano et al [48] c Calculated according to Facchiano et al [48] d Calculated using the program AVP sequence identity, ... with the expected mass calculated fromthe amino acid sequence The identity of the protein was checked by N-terminal sequencing and was conrmed by MALDI-MS analysis of the HPLC puried protein The ... using the crystal structure of CU-NH from S solfataricus Escherichia coli pyrimidine-specic NHs Yeik [29] and Ybek [30] as templates The structure provided insight into the active site architecture...
... (C) phosphocellulose, (D) dsDNA-cellulose, and (E) and ssDNA-cellulose columns The detailed description of each chromatographic procedure is given in the text The dotted line indicates the KCl ... activity of PDH120 by actinomycin C1 (B), ethidium bromide (C) , daunorubicin (D) and nogalamycin (E) The DNA helicase reactions were performed in the presence of increasing concentrations of the ... by including different concentrations of actinomycin C1 (Fig 6B), ethidium bromide (Fig 6C) , daunorubicin (Fig 6D), and nogalamycin (Fig 6E) in thestandard helicase reactions The titration curve...
... to the respiratory tract, cataracts, cancer 17 Health Effects of Other Pollutants Compound Mechanism Health Effect Volatile Organic Compounds Combine with NOx Significantly reduce lung function ... represents the highest excess cancer risk for a p g receptor fromthe refinery source category with a 70 year period exposure period taking into account the distance fromthe refinery to the receptor ... depressurization work practice standard for delayed coking units (DCU) The NOX limit for fluid catalytic cracking units (FCCU) The particulate matter (PM) limit for FCCU 10 EPA’s decision not to promulgate...
... the heme (Fig 4D) From solvent accessibility calculations using the program naccess v2.1.1 (http://wolf.bms.umist.ac.uk/naccess) the position of the K99 side chain in conformer A results in the ... solved by molecular replacement using the program molrep [56] fromthe CCP4 program suite [57] using the ferricyt c- 550 structure from P denitrificans (PDB code 1cot [15]) as the search model A solution ... studies of cyt c- 550 Fig Overlay of the main-chains of three bacterial c- type cytochromes with the highest structural homology to P versutus cyt c 550 Yellow P denitrificans cyt c- 550 (1cot, 1.7...
... CS060# and CS#60# Residue Carbon CS040# (p.p.m.) CS060# (p.p.m.) CS#60# (p.p.m.) DUA (C) C1 C2 C3 C4 C5 C6 C1 C2 C3 C4 C5 C6 CH3 C O C1 C2 C3 C4 C5 102.83 71.54 67.51 109.41 147.01 – 102.97 55.02 ... 4-sulfated GalNAc residue Table Carbon chemical shift Dd values between CS040# and CS060# Residue Carbon Calculated (p.p.m.) Observed (p.p.m.) DUA (C) C1 C2 C3 C4 C5 C1 C2 C3 C4 C5 C6 +1.1 +0.9 ... of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(b1– 3)GalNAc(b1-]n, which may be O-sulfated on the C4 and ⁄ or C6 of GalNAc and C2 of GlcA...
... statistics could not be used for other locations or Crop Broccoli (Brassica oleracea L.), lettuce (Lacuca dativa L.), and onion (Allium cepa L.) Soybean (Glycine max L Merr cv Clark) Cotton (Gossypium ... the United States The European approach has centered around the concept of a “critical level” (CL), which is based on a cumulative exposure above a cutoff concentration below which only an acceptable ... into the plant rather than just ambient O3 concentration or cumulative exposure This article focuses on research that has been conducted on the exposure of agricultural crops to enhanced concentrations...
... to consult your Visual C+ + documentation for details.) To compile MyProg.cpp using C+ + Builder, use this command line C: \ >bcc32 Sample.cpp The output from a C+ + compiler is executable object code ... Inheritance is the process by which one object can acquire the properties of another object The reason this is important is that it supports the concept of hierarchical classification If you think ... with CThe reason for this is simple: C+ + is built upon the foundation of C In fact, C+ + is a superset of C (Indeed, all C+ + compilers can also be used to compile C programs!) Specifically, C+ +...
... another member of the infantry, carries not only hatchet with which he cuts off a thumb of an enemy Harry Dobbins carried his girlfriend's panties around his neck, and Dave Jansen carried ... not now a factor" (288) O'Brien's use of the term "freedom birds" is appropriate when referring to the jets that take troops away because it carries them away, far away from where they don't want ... seemingly reaching out to him, she in fact had no deep feeling for him Lieutenant Jimmy Cross, in the end, realized the mistakes he's made He sees that he has unknowingly threatened the lives of the men...
... Jolla, CA, USA) with forward primer 5¢-GAAACGATGC ATTTGTCCTATGCCGACCGTGCGTC-3¢ and reverse primer 5¢-GACGCACGGTCGGCATAGGACAAATGCA TCGTTTC-3¢ The sequence of the second PCR product was also confirmed ... bound to the catalytic pocket As described above, for E coli GGT it has been well established that the catalytic reaction proceeds in the active site pocket, which is shielded from solvent by the ... anthracis CapD catalyzes the cleavage of the c- glutamyl bond but, unlike GGTs, transfers the poly -c- d-glutamic acid to the peptidoglycan cell wall, and is therefore involved in linking the capsule...
... interaction may be an artifact caused by the crystallization of lactose-liganded EW29Ch because: (a) in the other EW29Ch molecule of the crystal structure (each crystal contained two molecules ... increasing concentrations of each sugar For the progressive chemical-shift changes of EW29Ch under conditions of fast exchange on the chemical-shift timescale, 15N and 1HN chemical-shift changes ... interacting with the protein, because the resonance signals of the glucose residue overlapping with those of the galactose residue within the lactose affected the subtraction of the free induction...
... substrate to the carboxylase and also activates the enzyme The discovery of c- carboxylated conotoxins and, more recently, the cloning and characterization of the c- carboxylase from cone snails ... (5¢-CTCTGAGGGCGCCAAACATGTCG-3¢) and GSP2 (5¢-CGACATGTTTGGCGCCCTCAGAG-3¢) in 5¢- and 3¢-RACE PCR that employed a C textile RACE library as the template Amplification parameters were as indicated by the manufacturer ... Gla(3)–TxVI contain a motif –cCCS– that is found in four other Gla-containing peptides, TxVIIA fromC textile, c- PnVIIA fromC pennaceus, d7a fromC delessertii and as7a fromC austini [1,21,22,29] Conotoxins...
... 5¢-ATIACITGGGA (C/ T)ACITA (C/ T)TA (C/ T)(A /C) G-3¢; band D Fig 1A, 5¢-TT (C/ T)GA (C/ T)CA(A/G)ATITA (C/ T)CA (C/ T )C- 3¢; and a generic vector primer (T7 primer), 5¢-GTAATACGACTCACTATAGGGCG-3¢ PCR products ... (http://www.bdbiosciences.com/clontech/) and the genespeci c primer: 5¢-CCTCGTCAATGGAGTACTCGTAG CCATCAG-3¢ The amplified product was cloned in pCR2.1 as described for DNA sequencing DNA sequences were determined by standard ... (C- terminal) restriction sites: Forward, 5¢-CGCGCTGCA GGTCATGAGAAATATGAAGGA-3¢; Reverse, 5¢-GC GCGTCGACTGAATAGTTTTGCAAGACGTACTG-3¢ They were designed to allow the amplified sequences encoding the proproteins...
... Student Characteristics Definted: SES (Socioeconomic Status): An index composed of five components: 1) father's education; 2) mother's education; 3) parents' income; 4) father's occupation; 5) ... RACIAL/ETHNIC GROUP: Collapsed grouping based on respondent's answer to race/ethnic question The category "Hispanic" includes those who answered Mexican-American or Chicano, Puerto Rican, or other ... Latin American origin "Oriental or Asian-American" andl "Other" were excluded fromthe race/ethnic distribution ACHIEVEMENT/ABILITY: From information collected in the Student's School Record Information...
... reference For the glycosylated peptide, the molecular mass calculated fromthe chemical structure is still almost in the error range of the molecular mass calculated fromthe diffusion constant for ... 1 3C chemical shifts from random-coil values The latter were calculated on the basis of the random-coil values of completely denatured model peptides [15], which were corrected for the effects ... of the random-coil values for the a-protons, a-carbons and b-carbons Graphs show the difference between the chemical shifts Dd of Cam-HrTH-I and Cam-HrTH-II and the sequence corrected random coil...