... cells were loaded with calcein by incubating cells with 0.1 μM of Calcein-AM for 10 in 0.15 M NaCl-20 mM Hepes buffer, pH 7.4, with 0.1% BSA at 37°C followed by extensive washing with NaCl-Hepes ... prostate cancer cell line PC-3 and the immortalized, non-malignant prostate cell line 267B1 cells grown in serum-free RPMI1640 with 0.1% bovine serum albumin were treated with 0.1% DMSO or with or ... SEM of 3-4 independent observations with four replicates in each observation) *: P < 0.05, **: P < 0.01 comparing with or without Fe conditions by 2way ANOVA with Bonferroni’s posttests In iron...
Ngày tải lên: 10/08/2014, 10:21
Differences Between ActionScript 1.0 and 2.0
... 1.0 and ActionScript 2.0, but this brief overview should provide you with enough insight to get started if you've worked with ActionScript 1.0 < Day Day Up > ... you more control over setting a variable's data type Here's how it works When creating a variable with ActionScript 2.0, you use this syntax not only to create the variable, but also to assign its ... can all those extra keystrokes be an enhancement? Well, there are several ways If you're familiar with the Actions panel's ability to provide code hints (which we will discuss shortly), you'll be...
Ngày tải lên: 24/10/2013, 12:15
listen and speaking 1 and 2
... page 194 Blond woman with glasses = 40 openings Young man in tie = 50 openings Brunette woman with glasses = 240 openings Woman wearing a white shirt = 2002 openings Woman with short, blond hair ... intelligent guesses and to become more comfortable with some ambiguity in their understanding This will assist them in being more successful in their encounters with English outside your classroom Using ... topics can be done in several ways with students working individually, in pairs, small groups, or as a whole class Chapter Overview Title page photo or art with accompanying activity: The photo...
Ngày tải lên: 14/12/2013, 15:07
... have audible and visual alarms associated with them when specified preset limits are exceeded These temperatures may have control functions associated with them so that equipment is started or ... bridge current The regulated current is divided between the branch with the fixed resistor and range resistor R1, and the branch with the RTD and range resistor R2 As the electrical resistance of ... tip of the tube traces a curve that is the result of the change in angular position with respect to the center Within limits, the movement of the tip of the tube can then be used to position a...
Ngày tải lên: 28/12/2013, 22:07
(1) how learners approach learning, both in and out of classrooms, and (2) the kinds of strategies and cognitive processing they use in second language acquisition
... and evaluate their own performance Learners should contact with native speakers and cooperate with them Learners must become acquainted with the target culture (Stern 1992: 265-266) e Affective ... (C3), reading without looking up every new words (C4) and using words and phrases with the same meaning (C6) were the most frequently used compensation strategy among the students with 58.3 %, ... results of Chang, Liu & Lee’s 49 (2007) study with Taiwanese college EFL students, Leyla’s (2004) study with Turkish adult students and Lee’s (2003) with Korean students Affective strategy was...
Ngày tải lên: 29/01/2014, 00:23
Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx
... NaCl) supplemented with 0.05% (v/v) Tween-80 (TBS-T), preincubated for h at room temperature with blocking buffer [5% (w/v) skimmed milk powder (Oxoid) in TBS-T], supplemented with 0.5 mM Na3VO4, ... mean 3908 J J Hornberg et al (Eur J Biochem 271) with TBS-Triton (TBS, supplemented with 0.1% (v/v) Triton X-100) Cells were then incubated with 100% (v/v) methanol for 10 at )20 °C in order ... TBS-Triton (1 : 400) Cells were washed for 15 with TBS-Triton, for 15 with 0.1% (w/v) BSA in TBS-Triton and incubated for h at room temperature with Cy5TM-labeled goat anti-(mouse Ig) (Amersham)...
Ngày tải lên: 19/02/2014, 16:20
Alfred Russel Wallace: Letters and Reminiscences, Vol. 1 (of 2) pot
... portion seen perfectly with both eyes is less than with one Thus [see diagram on next page] we only see from A to A with both our eyes, the two side portions Ab Ab being seen with but one eye, and ... me with admiration A paradoxical mixture of sound and silence pervades the shady parts of the wood yet within the recesses a universal silence appears to reign such a day as this brings with ... upon his own tact and patience, combined with firmness, in his dealings with the natives On one occasion he was taken ill, and had to remain six weeks with none but native Papuans around him,...
Ngày tải lên: 06/03/2014, 23:21
Casa Braccio, Volumes 1 and 2 (of 2) potx
... warm with the afternoon sun Of what use was life, if it was to be lived in the tomb with the accompaniment of a lifelong funeral service? Why should not God be as well pleased with suicide as with ... live half dead, and to praise Him with voices that crack with the cold in winter, and to kneel till we faint with the heat in summer, and to wear out our bodies with fasting and prayer and penance, ... cried Annetta, with real or feigned astonishment, and she tossed a knife and fork angrily into a plate, with a loud, clattering noise "I am speaking with you," answered her father, without raising...
Ngày tải lên: 17/03/2014, 13:20
Báo cáo khoa học: Barley polyamine oxidase isoforms 1 and 2, a peculiar case of gene duplication docx
... site within the gene sequence A HvPAO1 promoter sequence subclone inserted in pGEM-Teasy vector was digested with EcoRI and filled in at its extremities, then cloned in pHTT515 previously cut with ... hydroxylapatite column equilibrated with the dialysis buffer The column was washed with 0.2 m NaH2PO4 containing NaCl 0.1 m, until the A280 decreased to zero The enzyme was eluted with 0.5 m sodium phosphate ... equilibrated with the dialysis buffer The column was washed with 50 mm sodium phosphate buffer, pH 5.0, containing 0.1 m NaCl, until the A280 decreased to zero The enzyme was eluted with 50 mm...
Ngày tải lên: 23/03/2014, 10:21
luận văn ứng dụng sử dụng mô hình web là “kiến trúc mô hình một và hai” – “jsp model 1 and 2 architecture”
... "legiang" %> Tag With Body Tag With Body ... paramtag.jsp (tiếp theo) Tag With Attributes
Ngày tải lên: 27/03/2014, 22:31
Báo cáo khoa học: The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2 pot
... interacts with scaffold attachment factor B1 (SAFB1) [27] SAFB1 is a protein of the nuclear matrix first discovered approximately a decade ago It was reported with different names and was associated with ... cells were co-transfected with plasmids expressing FLAG, FLAG–SRPK1 or FLAG–SRPK1a together with GFP, GFP–SAFB1 or GFP–SAFB2 Whole-cell extracts were immunoprecipitated with an anti-FLAG IgG A) ... centrifugation and washed three times with immunoprecipitation buffer For co-immunoprecipitation of the kinase with the SAFB proteins, extracts of HeLa cells transfected with equal quantities of plasmids...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: Role of extracellular signal regulated kinases 1 and 2 in neuronal survival docx
... role of ERK1/2 in neuronal survival after exposure to DNA-damaging anticancer agents suggests that neurotoxicity induced during cancer treatment may be enhanced by drugs affecting protective ERK1/2 ... in neuronal protection in status epilepticus Inhibition of ERK1/2 activation with SL327 increased mortality in rats with pilocarpine-induced seizures [35] Ó FEBS 2004 Interestingly, in several ... was implicated as one of the killer mechanisms activated by NGF withdrawal in this system If trophicdeprived cells were rescued with NGF, Bim protein levels decreased This effect was mediated...
Ngày tải lên: 30/03/2014, 14:20
Diderot and the Encyclopædists (Vol 1 of 2) docx
... future, with cuffs dirty and torn, with holes in their stockings and holes in their shoes, with hair all unkempt, in shabby overcoats with many rents, or scanty black suits with starting seams, with ... quite content with life as it came If he found himself absolutely without food and without pence, he began moodily to think of abandoning his books and his pen, and of complying with the wishes ... father, "I am come to see if you are well, if you are satisfied with your superiors, with your food, with your companions, and with yourself If you are not well or not happy, we will go back...
Ngày tải lên: 31/03/2014, 18:20
solutions to the class 1 and 2 problems in transport phenomena (wiley_ 1960)(175s)
Ngày tải lên: 02/04/2014, 16:45
andrew s. glassner - principles of digital image synthesis vol 1 and 2
Ngày tải lên: 05/06/2014, 11:42
báo cáo hóa học: " Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes" pdf
... DNA sequence for HIF-1 binding in the hypoxia-response element is 5'-[A/ G]CGTG-3' flanked with or without a second consensus site 5'-[A/C]ACAG-3' [12] Mutations of the consensus sequences result ... Inc (CAT # sc-2625, Santa Cruz, CA) and end-labeled with γ[32P]ATP (Mandel/NEN Life Science, Guelph, ON) Nuclear proteins (5 μg) were incubated with μg poly-d [I-C] (Amersham Biosciences, Montreal, ... PCR fragment was cleaved with KpnI and BglII (Invitrogen) and cloned into a luciferase yellow reporter gene vector pGL3-promoter vector (Promega Madison, WI) cleaved with the same enzymes The...
Ngày tải lên: 19/06/2014, 22:20
Báo cáo hóa học: " Fabrication and ultraviolet photoresponse characteristics of ordered SnOx (x ≈ 0.87, 1.45, 2) nanopore films" potx
... bending and recombine with the negatively charged adsorbed oxygen ions [h++O2- ® O2(g)], resulting in an enhancement of photocurrent When the illumination is turned off, the films with higher surface ... fixed at V The structural properties were determined using a D8 DISCOVER X-ray diffractometer (XRD) with Cu Κa radiation The growth and surface morphologies were observed using a field-emission scanning ... Raman spectra of the SnOx nanostructures were measured using a Jobin Yvon LabRam HR 800 UV system with a 325 nm HeCd laser Results and discussions Surface topography Figure 2a, b is the top view...
Ngày tải lên: 20/06/2014, 23:20
Báo cáo hóa học: "Research Article Time-Frequency and Time-Scale-Based Fragile Watermarking Methods for Image Authentication Braham Barkat1 and Farook Sattar (EURASIP Member)2 1 Department 2 Faculty" pdf
... noise (This type of noise is typically seen on images with impulse noise model and represents itself as randomly occurring white and black pixels with value set to 255 or 0, resp.) Specifically, we ... Verification without Resorting to the Original Watermark Here we present how to check the integrity of the watermarked image content, and how to localize any tamper in the image, without knowing ... SNR decreases) with decreasing quality factor values In the same table, we also show the PSNR values obtained in this case These values confirm the degradation of the attacked image with decreasing...
Ngày tải lên: 21/06/2014, 08:20
CCNA 1 and 2 Companion Guide, Revised (Cisco Networking Academy Program) part 110 ppt
... managing Cisco IOS Software with ROMmon, 662–667 with TFTP, 660–661 Manchester encoding, 318 MANs (metropolitan-area networks) versus LANs and WANs, 52 mapping networks with CDP, 627 Mb (megabit), ... ip access-group command, 855 IP addresses, 32–33, 386 ARP, 408 default gateways, 411 operation within subnets, 410 assignment of, 407 BOOTP assignment, 414–415 DHCP assignment, 415–417 RARP assignment, ... 1102.book Page 1063 Tuesday, May 20, 2003 2:53 PM Layer 1063 bridges, 82 cabling, 205–206 comparing with WANs, 511 connection devices bridges, 218–219 hubs, 217 repeater, 216 switches, 221–222 connectivity,...
Ngày tải lên: 04/07/2014, 18:20