bone stromal cells as therapeutic targets in osseous metastasis
Báo cáo khoa học: Human telomeric G-quadruplex: The current status of telomeric G-quadruplexes as therapeutic targets in human cancer pdf
Ngày tải lên :
06/03/2014, 09:22
... -(CH2)3- with a terminal cationic nitrogen-containing group such as diethylamine, pyrrolidine or piperidine Structure-based drug discovery does have these few structures as starting points [42–47], ... T, Shin-ya K, Sashida G, Sumi M, Okabe S, Ohyashiki JH & Ohyashiki K (2006) Telomerase inhibition with a novel G-quadruplex-interactive agent, telomestatin: in vitro and in vivo studies in acute ... [30,31] This was indeed the observation in dominant-negative telomerase transfection experiments, but would be therapeutically challenging for human cancer treatment Initial findings using G-quadruplex...
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Chemokines as therapeutic targets in systematic inflammatory response syndrome
Ngày tải lên :
11/09/2015, 16:04
... suppression As inhibiting Fas-FasL signaling (e.g., Fas fusion protein (Chung et al., 2003), or Fas siRNA administration (Wesche-Soldato et al., 2005)), caspase inhibition (caspase gene deficiency and inhibitor ... IkappaB kinase kinases IL Interleukin JNK c-Jun N-terminal kinases LPS Lipopolysaccharide MAPK Mitogen-activated protein kinases XIV MCP-1 Monocyte chemotactic protein-1 MIP-1a Macrophage inflammatory ... feeding also induces a decrease in caspase expression and an increase in cathepsin B expression, indicating that ethanol abuse may prevent apoptosis of pancreatic cells and activate trypsinogen...
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Báo cáo khoa học: "Eag and HERG potassium channels as novel therapeutic targets in cancer" docx
Ngày tải lên :
09/08/2014, 03:24
... enhances firing and epinephrine secretion in rat chromaffin cells: the missing link to LQT2-related sudden death? FASEB J 2003, 17(2):330-2 96 Rosati B, et al: Glucose- and arginine-induced insulin secretion ... channels Increased expression of K+ channels on cell membrane results in increased influx of Ca2+ ions resulting in increased transition of cells through G1/S phase of cell cycle The channels in presence ... role in prolactin secretion [58] Further investigation of HERG channels in cancer invasion and metastasis revealed that, in addition to the high expression of herg gene and HERG protein in colorectal...
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ONCOGENOMICS AND CANCER PROTEOMICS – NOVEL APPROACHES IN BIOMARKERS DISCOVERY AND THERAPEUTIC TARGETS IN CANCER pot
Ngày tải lên :
30/03/2014, 09:20
... activated protein kinase (MEK) inhibitor such as CI-1040 was used to test the potential therapeutic agent in in vitro ovarian cancer cell line (51) This cell lines containing KRAS or BRAF mutations, ... tissues in the breast Noninvasive or also known as in situ’ indicates that the cancerous cells have not yet invaded other tissues within the breast There are several grading systems used to classify ... variant was found to be associated with increased breast cancer risk in all Chinese in Tianjin, Nanjing, Taiwan and Hong Kong This was also in agreement with three studies conducted in Japanese...
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Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
Ngày tải lên :
09/08/2014, 10:23
... as reported previously [16] Decreased and bones of TNF-transgenic mice stromal cellsstromal cell-derived factor-1 expression in bone marDecreased stromal cell-derived factor-1 expression in bone ... Figure cells inhibits stromal cell-derived factor expression by stromal TNF inhibits stromal cell-derived factor expression by ST2ST2 stromal cells ST2 cells, a bone marrow stromal cell line, were ... of CXCR4+ stained CD11b+ cells shown Black line, CXCR4 CKO cells; red line, control cells (b) Murine TNF (0.5 μg/injection, times/ day × days, intraperitoneally) or PBS was injected into 2-month-old...
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Báo cáo y học: "Intervertebral disc cells as competent phagocytes in vitro: implications for cell death in disc degeneration" pptx
Ngày tải lên :
09/08/2014, 10:23
... peroxidase and diaminobenzadine as the substrate and enhanced with avidin-biotin complex (Vector Laboratories, Burlingame, CA, USA) Mouse IgG was used as a negative control Immunopositivity was assessed ... medium (Invitrogen Corporation) supplemented with 10% FBS The J774 cell line (derived from murine macrophages, kindly donated by Robin May, University of Birmingham, UK) was maintained in DMEM/F-12 ... removing potential matrix-degrading enzymes, phagocytosis can trigger the release of antiinflammatory cytokines such as transforming growth factorbeta whilst inhibiting the production of proinflammatory...
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Báo cáo y học: " Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+ bone marrow cells, following gamma irradiation in cynomolgus macaques" potx
Ngày tải lên :
13/08/2014, 05:21
... resulting in the formation of clusters of GFP-positive cells within a single myeloid colony In our in vivo study, autologous HSC were injected into the bone marrow, whereas intravenous injection ... very few transduced cells in the bone marrow and SIV-DNA was not detected in peripheral blood cells In the three remaining animals, vector DNA was detected in peripheral blood cells (up to 500 copies ... cells with three plasmids: A a plasmid pGASE containing the eGFP gene under control of the PGK promoter; B a plasmid pSIV3+ containing viral genes; C a plasmid pGREV containing the VSV envelope...
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Exploring mesenchymal stem cell derived exosome and tocotrienol (t3) as therapeutic agents in drug induced liver injury (DILI)
Ngày tải lên :
09/09/2015, 11:21
... APAP APR AST ATP TTP BSA BTI CAT Apoptosis-inducing factor Alkaline phosphatase Alanine aminotransferase Activating protein-1 Acetaminophen (paracetamol) Acute phase response Aspartate aminotransferase ... TNF- TNFR1 TP Interleukin Inducible nitric oxide synthase Insulin, transferrin, selenium c-Jun-N-terminal kinase Liver function test Lipid peroxidation Mitogen activated protein kinase Monochlorobimane ... Proteins Removal the source of injury Healing and repair injury Maintain homeostasis Figure Acute phase response and induction of acute-phase protein expression by TNF- cytokines and IL6 cytokines...
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Báo cáo y học: "B cells as a therapeutic target in autoimmune disease" doc
Ngày tải lên :
09/08/2014, 01:21
... further increasing the antibody’s depleting activity [7] Rituximab has been used in the treatment of B-cell non-Hodgkin’s lymphoma as a single agent as well as in combination therapy, emphasizing ... immunoglobulin levels are usually maintained, possibly as a consequence of plasma cells being spared B-cell depletion in antibody-mediated diseases It is understandable that rituximab has been most ... confirm that, at least in some patients, plasma cells are not sufficient to maintain autoantibody levels and that continuous B-cell recruitment and activation are necessary to maintain autoantibody...
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Báo cáo y học: "Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis" ppsx
Ngày tải lên :
12/08/2014, 17:21
... was intraoperatively obtained from the nine RA patients and the 10 OA patients while undergoing joint arthroplasty Detailed patient characteristics, medication usage, including disease-modifying ... cascade, protein kinase cascade, and activation of mitogenactivated phosphate kinase (MAPK) Twenty-six of the 33 genes in phosphate metabolism belonged to protein amino acid phosphorylation Finally, ... telangiectasia mutated; BM: bone marrow; BMMC: BM-derived mononuclear cells; CCN: cyclin; CDC: cell division cycle; CDK: cyclindependent kinase; CRP: C-reactive protein; DMARDs: disease-modifying antirheumatic...
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Báo cáo sinh học: " In vitro evaluation of a double-stranded selfcomplementary adeno-associated virus type2 vector in bone marrow stromal cells for bone healing" pptx
Ngày tải lên :
14/08/2014, 19:22
... types including dividing and non-dividing cells [20] In addition, there is no conclusive evidence indicating pathogenicity of AAV vector in humans [21] AAV also induces long-term gene expression in ... been used as delivery methods in gene therapy and each serotype has a distinct affinity for certain cell types that is because of differences in cell binding and/or intracellular trafficking [21] ... human and rat bone marrow stromal cells it did not show GFP expression as strongly as transduced 293T cells at the same time point In contrast, strong GFP expression was detectable in all three...
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Tài liệu Báo cáo khoa học: Trophoblast-like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins pdf
Ngày tải lên :
20/02/2014, 23:20
... (nonspecific binding) [37] Following this incubation the medium was aspirated and the cells were washed twice with HBSS containing 5% (v/w) bovine serum albumin followed by two washes in HBSS Cells were ... maintained in medium containing 0.5 mgÆmL)1 G-418 Both cell lines were kindly provided by Dr M Krieger (MIT, Cambridge, MA, USA) Binding studies Binding studies of human HDL3 to choriocarcinoma ... streptomycin) and the incubation was continued for 36 h without rocking Control cells were infected with b-gal virus as described for SR-BI infected cells The expression rate of SR-BI was determined...
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Báo cáo khoa học: Receptor- and calcium-dependent induced inositol 1,4,5-trisphosphate increases in PC12h cells as shown by fluorescence resonance energy transfer imaging pot
Ngày tải lên :
30/03/2014, 03:20
... restored the intracellular calcium concentration, the increase in Ins(1,4,5)P3 was sustained In addition, the subsequent increase in calcium was less effective at enhancing the increase initial receptor-activated ... of these calcium increases were accompanied by increased Ins(1,4,5)P3, indicating that calcium can enhance receptor-induced Ins(1,4,5)P3 increases The peak Ins(1,4,5)P3 increases were similar ... receptorinduced Ins(1,4,5)P3 increases in CHO cells expressing m1 mAChR PC12h cells express m1 mAChR and CCh-induced increases in calcium and investigate the cell-type dependence production, we assayed...
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Báo cáo sinh học: "CGB and GNRH1 expression analysis as a method of tumor cells metastatic spread detection in patients with gynecological malignances" potx
Ngày tải lên :
18/06/2014, 22:20
... Laboratories GmbH, Pasching, Austria) were used to separate the cells during centrifugation at 1200 × g for 20 minutes at room temperature in a swinging bucket rotor Cells located in the interphase were ... (Table 3) as well as clinical data (Table 4) in studied tissues and blood was observed Discussion The critical role of circulating tumor cells in metastatic spread of carcinomas has already been ... silencing, showing that reduction of the hormone’s expression in vitro resulted in increased apoptosis rate of cancer cells [36] Furthermore elevated CGB level in serum was found to be associated...
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Báo cáo y học: " Immunohistochemical Characteristics of Bone Forming Cells in Pleomorphic Adenoma" ppt
Ngày tải lên :
08/08/2014, 16:23
... mesemchymal cells of true stromal tissue, including the possibility that one of the metaplastic factors was neoplastic cells producing BMP Therefore, we sought to determine the origin of the bone forming ... discussed bone formation mechanisms according to the histopathological findings of their bone forming pleomorphic adenoma case In their report, the bone tissue was formed in the so-called stromal ... bone forming cells in our present case using immunohistochemical technique with S-100 protein as a marker of myoepithelial cells, and Runx2 as a marker of bone forming cells S-100 protein is a well-known...
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Báo cáo y học: "Garden of therapeutic delights: new targets in rheumatic diseases" pptx
Ngày tải lên :
09/08/2014, 01:22
... protein kinases Mitogen-activated protein (MAP) kinases are stress-activated serine/threonine kinases that include the p38, ERK (extracellular regulating kinase), and JNK (c-Jun-N-terminal kinase) ... c-Jun N-terminal kinase; MAPKAPK, mitogen-activated protein kinase-activated protein kinase; MEK1/2, mitogen-activated protein kinase kinases types p38 inhibitors are effective in preclinical models ... respectively The cascade generally has three levels (shown on the left), including the MAP kinase kinase kinases (MAP3Ks), which activate the MAP kinase kinases (MAPKKs or MKKs), which, in turn, activate...
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Báo cáo y học: "The role of statins as potential targets for bone formation" pptx
Ngày tải lên :
09/08/2014, 03:24
... Mundy Figure In vitro effects Simvastatin, mevastatin and atorvastatin (but not pravastatin) were found to have identical effects to those seen with lovastatin Cerivastatin, however, was 10–100-fold ... statin use, bone mineral density and subsequent fractures [19] Since then a study in postmenopausal women has been published that indicated a significant increase in bone mineral density associated ... Further investigations found that statins stimulate bone formation both in vitro and in vivo in animal models of osteoporosis associated with increased expression of the BMP-2 gene in bone cells...
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Báo cáo y học: "Targeting Toll-like receptor signaling in plasmacytoid dendritic cells and autoreactive B cells as a therapy for lupus" pps
Ngày tải lên :
09/08/2014, 07:20
... helicases and/or topoisomerases) in order to bind more avidly to the TLR9 and to initiate downstream signaling Interestingly, unprimed follicular B cells, in contrast to marginal zone (MZ)-B cells, ... immunoglobulin switch regions) These motifs can block TLR9-induced activation in both a cis and trans manner Surprisingly, chromatin-containing immune complexes in lupus sera were capable of inducing ... indispensable role in lupus pathogenesis Interestingly, IFN priming induces higher IL-6 and IL-10 secretion from CpGDNA stimulated MZ-B cells [110] Although MZ-B cells, at least in murine lupus, may...
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Báo cáo y học: "High mobility group box-1 protein as a tumor necrosis factor-independent therapeutic target in rheumatoid arthritis." ppsx
Ngày tải lên :
09/08/2014, 10:23
... patients, remained unchanged in one, and increased in three, rendering the authors’ results statistically insignificant HMGB1 mRNA expression remained unchanged after infliximab therapy as indicated ... arthritis upon rHMGB1 administration, and the improvement of disease after HMGB1 inhibition implicate HMGB1 as a key mediator and potential therapeutic target in RA HMGB1 as a novel therapeutic The recent ... arthritis in animals and shares key histological features observed in RA, including pannus formation, synovial membrane hypertrophy, and mononuclear cell predominance within synovial tissue [7] The injection...
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Báo cáo y học: "Biomarkers as tools for improved diagnostic and therapeutic monitoring in systemic lupus erythematosis" potx
Ngày tải lên :
09/08/2014, 14:22
... association with lupus disease activity indices or other clinical features was determined High IFN scores were positively associated with increased disease activity as indicated by the SLEDAI and ... longitudinal analysis of individual patients indicated that ICOS was significantly elevated during the active phase when compared with clinical remission Sialic acid binding immunoglobulin-like lectin ... validated as a predictor of clinical response At least one pharmaceutical company is applying the IFN signature as a rapid indicator of clinical response in early phase I/II trials targeting IFNα...
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