bone reconstruction utilizing mesenchymal stem cell sheets for cell delivery

... Markers of stemness in equine mesenchymal stem cells: a plea for uniformity Theriogenology 2011 May;75(8):1431-43 PubMed PMID: 21196039 72 Ulloa-Montoya F, Verfaillie CM, Hu WS Culture systems for pluripotent ... to three times a week Cell sheets were formed in the presence of Ascorbic acid (Passage 1) and for each surgery, at least cell sheets were prepared and around two million cells /cm2 (which is ... cultured cells were mesenchymal stem cells Saline that was used for transporting the cartilage biopsy to the laboratory, aspirated bone marrow and culture media (without antibiotic) was tested for...

Ngày tải lên: 10/09/2015, 09:01

... cells Pancreatic stem cells Intraislet, nestin-positive cells, oval cells, duct cells Beta cells Skletal-muscle stem cells or satellite cells Muscle fibers Skeletal muscle fibers Stem cells of the ... mature blood cell components Mesenchymal stem cells Bone marrow, peripheral blood Bone, Cartilage, tendon, adipose tissue, muscle, marrow stroma, neural cells Neural stem cells Ependymal cells, astrocytes ... iv   1.5 Types of stem cells 14   1.5.1 Embryonic stem cells (ESCs) 15   1.5.2 Induced pluripotent stem (iPS) cells 15   1.5.3 Adult stem cells 16   1.5.4 Heterologous stem cells 17   1.6 MSCs...

Ngày tải lên: 10/09/2015, 09:10

... culture media would increase the intracellular iron content of MSCs 61   Average iron content per cell (pg /cell) 200 150 100 50 Average iron content per cell (pg /cell) 0µg/ ml 25µg/ ml 50µg/ ml 75µg/ ... help the distribution of the cells in 51   the joint, passive flexion/extension of the joint were performed MRI scanning was performed on the joint at 2- weekly interval for up to weeks postoperation ... than 98% of cells were negative for hematopoietic markers (CD14, CD31, CD34, and CD45), and endothelial marker (CD31) (figure 3.3) 58     Figure 3-3 Flow cytometry analysis of the stem cells surface...

Ngày tải lên: 10/09/2015, 09:10

... labeled cells (the average amount of the iron nanoparticles per cell was assumed as 10ng /cell; e.g 1, 10, 1000 µg iron nanoparticles were mixed with agarose as representative of 100, 1000, 100000 cells) ... the MSCs did not affect the proliferation rate of the cells over time 3.4.6 Differentiation potential of labeled MSC MSCs are multipotent stem cells, which can differentiate to different lineage ... pig’s knee explants with blank and scaffold only defect (A), 100 cells simulation (B), 1000 cells simulation (C), and 100,000 cells simulation (D) Left panel of each image is 3D-FSE sequence...

Ngày tải lên: 10/09/2015, 09:11

... labeled cell localization data (Arrowheads show the distribution of the labeled cells in the knee joint) MSCs were distributed in different places of the knee (figure 3.14) The labeled cells migrated ... mini-pig’s femoral condyle before defect creation (normal), one week after making the defect (before injection), immediately after injection, two and six weeks after the cells injection Upper panel ... site and arrows demonstrate the labeled stem cells) 72     Figure 3-16 MRI of the distribution of the labeled MSCs in the mini-pig knee joint The labeled cells migrated to the para-patellar fat...

Ngày tải lên: 10/09/2015, 09:11

Ngày tải lên: 10/09/2015, 09:11

... could detect labeled cells in vivo up to weeks, thus the SPIO labeling method of the cells can be a promising tool for real time evaluation and tracking of MSCs, providing data for a better understanding ... (189) This difference could be the result of lower loading of the cells (5 pg iron per cell) by Heymer et al., which is similar to our cells iron content labeled with lower labeling concentration ... MSCs is indeed feasible in vivo Cells migration and localization into different site of the inflammation was observed in the knee joint over time A majority of the cells moved to the surgery sites...

Ngày tải lên: 10/09/2015, 09:11

... it allows for integration of biophysical and biochemical factors essential in mimicking physiological conditions for cells after administration Key words: Mesenchymal stem cell (MSC), cell migration, ... mosaicplasty, and cell based therapy (autologous chondrocyte implantation, or matrix associated cell implantation)(29) Recently, cell based therapy such as mesenchymal stem cells (MSCs) become ... Tumor necrosis factor Transforming growth factor beta Transforming growth factor beta Transforming growth factor beta Bone morphogenetic protein Bone morphogenetic protein Bone morphogenetic protein...

Ngày tải lên: 10/09/2015, 09:11

... particular that of stem cells, and the biomaterials, for cartilage tissue engineering will be addressed 2.3.1 Stem cell based Approaches for Cartilage Tissue Engineering The aforementioned weaknesses ... hypertrophy and cell dedifferentiation, but donor site morbidity and fibrocartilage formation are still inevitable drawbacks Thus, mesenchymal stem cell (MSC) as an alternative cell source is ... encourage the investigation on stem cells with chondrogenic potential for articular cartilage repair Both pluripotent and multipotent stem cells have been reported for studying cartilage repair(Hwang...

Ngày tải lên: 09/09/2015, 08:12

... titanium substrates Culture of bone marrow-derived stromal cells (BMMSC) 57 Culture of bone chip-derived osteoblasts (BC-OB) 58 Culture of human mesenchymal stem cells (hMSC) 59 (Lonza, USA) Culture ... immobilized bone morphogenetic protein-2, 2nd International Congress on Stem Cells and Tissue Formation, 2008, Dresden, Germany 16 Chapter General Introduction The initiative for the modification of bone ... heterogeneous mesenchymal stromal cells [69] These cells can proliferate in vitro as plastic-adherent cells MSCs have a fibroflast-like morphology, can form colonies and can differentiate into bone, ...

Ngày tải lên: 14/09/2015, 08:37

... human mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications Curr Stem Cell ... Kitamura S & Nagaya N (2007) Effect of hypoxia on gene expression of bone marrowderived mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan ... of transplanted mesenchymal stem cells in a rat model of global heart failure Mol Biol Rep 36, 725–731 Li L, Zhang Y, Li Y, Yu B, Xu Y, Zhao S & Guan Z (2008) Mesenchymal stem cell transplantation...

Ngày tải lên: 18/02/2014, 04:20

... Therapeutic potential of mesenchymal stem cells producing interferonalpha in a mouse melanoma lung metastasis model, Stem Cells 26, (2008), 2332-8 Satija, N K et al., Mesenchymal stem cell- based therapy: ... cell- based therapy: a new paradigm in regenerative medicine, J Cell Mol Med 13, 2009 STEM CELL RESEARCH USING MESENCHYMAL (MESENCHYMAL STEM CELL - MSC) IN THE TREATMENT OF CANCER AGENCY - RECTUM RATS ... some characteristics of mesenchymal stem cells in cancer immunotherapy in mice Research results show that the mesenchymal stem cells injected into mice at the dose of 107 cells/ml can inhibit the...

Ngày tải lên: 20/03/2014, 09:21

... availability of various cellular sources for experimental investigations [6,7,10-15] including bone marrow-derived mesenchymal stem cells (BMDMSCs), hematopoietic stem/ progenitor cells, and cells of embryonic ... adipose-derived mesenchymal stem cells (ADMSCs) After culturing for 14 days, majority of isolated adipose-derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = ... staining, the cells were fixed with 1% paraformaldehyde Flow cytometric analyses were performed by utilizing a fluorescence-activated cell sorter (Beckman Coulter FC500 flow cytometer) Cell viability...

Ngày tải lên: 18/06/2014, 19:20

... of mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem ... hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem cells (ESC), mesenchymal stem cells (MSC), and various tissues ... serum free conditions for 14 days (Figure 3B) For co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated...

Ngày tải lên: 10/08/2014, 05:21

... of bone marrow-derived MSCs Flow cytometric analyses of bone marrow-derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 Figure Effect of simvastatin and bone ... al, which impair the stem cell functions to varying degrees, likely limiting the efficiency of stem cell therapy Therefore an approach to augment the angiogenic potency of bone marrow-derived ... from bone marrow by aspiration and expanded ex vivo before implantation Under specialized culture conditions, bone marrow-derived MSCs have the capacity to differentiate into cells such as bone, ...

Ngày tải lên: 10/08/2014, 05:21

... H, Seuwen K: Stem cell characteristics of human trabecular bone- derived cells Bone 2002, 30:699-704 De Bari C, Dell’Accio F, Tylzanowski P, Luyten FP: Multipotent mesenchymal stem cells from adult ... human mesenchymal stem cells generate osteosarcoma-like lesions in the lung Stem Cells 2007, 25:1586-1594 doi:10.1186/1423-0127-18-54 Cite this article as: Chang et al.: Mesenchymal stem cell ... Umbilical Cord Blood-Derived Mesenchymal Stem Cell Transplantation in Experimental Lupus Nephritis Cell Transplant 2011, 20:245-257 32 Parekkadan B, Milwid JM: Mesenchymal stem cells as therapeutics...

Ngày tải lên: 10/08/2014, 10:20

... Immunocytometry Systems, Mountain View, CA) For intracellular antigen detection, cells were first fixed in 2% paraformaldehyde (Sigma) for 15 minutes at 4°C and permeabilized with 0.1% saponin (Sigma) for ... injury Besides HSCs, there also existed another kind of stem cells called MSCs (Mesenchymal Stem Cells), they could differentiated into stroma cells and acted as the “niche” in the micro-environment[24] ... (1-20 nM) cells cultured for up days were used as templates for PCR reactions using specific primers for MMP-9 and ICAM-1 (B) The cDNAs from snc-RNA (20 nM) and ds-RNA (20 nM) cells cultured for up...

Ngày tải lên: 10/08/2014, 10:21

... pericytes? Cell Stem Cell 2008, 3:229-230 da Silva ML, Caplan AI, Nardi NB: In search of the in vivo identity of mesenchymal stem cells Stem Cells 2008, 26:2287-2299 Caplan AI, Bruder SP: Mesenchymal stem ... perivascular origin for mesenchymal stem cells in multiple human organs Cell Stem Cell 2008, 3:301-313 Delorme B, Chateauvieux S, Charbord P: The concept of mesenchymal stem cells Regen Med 2006, ... Transplantation of bone marrow stromal cells for treatment of central nervous system diseases Adv Exp Med Biol 2006, 585:49-64 10 Le Blanc K, Ringden O: Immunomodulation by mesenchymal stem cells and...

Ngày tải lên: 11/08/2014, 03:20