bone marrow peripheral blood stem cell
Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
... simvastatin on the cell viability of bone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under hypoxia stress, cells viability was ... of bone marrow- derived MSCs Flow cytometric analyses of bone marrow- derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 Figure Effect of simvastatin and bone ... of bone marrow- derived stem cells in the treatment of ischemic hindlimb Overall, this study demonstrates more pronounced angiogenic response, decreased muscle cell apoptosis and improved blood...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 10
... the stem cells and/or attract the endogenous or exogenous stem cells (endogenous from the bone marrow and exogenous by multiple intra-articular injection of the expanded autologous stem cells) ... (265, 266), or introducing new cell sources like debrided waste chondrocytes, Bone Marrow derived Mesenchymal Stem Cells (BM MSCs), or any combination of these cells (29, 214, 215, 267, 268) ... the bone marrow and expanded the cells the same as Wakitani’s approach however, we prepared the cell sheets (by useing Ascorbic acid) instead of cell suspension Seventy milliliters of venous blood...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 1
... mature blood cell components Mesenchymal stem cells Bone marrow, peripheral blood Bone, Cartilage, tendon, adipose tissue, muscle, marrow stroma, neural cells Neural stem cells Ependymal cells, astrocytes ... adult stem cells and their differentiation capacity (76) Cell type Tissue-Specific location Cells or tissues Produced Hematopoetic stem cells Bone marrow, peripheral blood, cord blood All mature blood ... cells Pancreatic stem cells Intraislet, nestin-positive cells, oval cells, duct cells Beta cells Skletal-muscle stem cells or satellite cells Muscle fibers Skeletal muscle fibers Stem cells of the...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 2
... culture media would increase the intracellular iron content of MSCs 61 Average iron content per cell (pg /cell) 200 150 100 50 Average iron content per cell (pg /cell) 0µg/ ml 25µg/ ml 50µg/ ml 75µg/ ... cytoplasm of the labeled cells 3.4.4 Iron content quantification in labeled-MSCs As shown in figure 3.6, mean iron content in labeled cells ranged from to 141 pg per cell The results showed ... than 98% of cells were negative for hematopoietic markers (CD14, CD31, CD34, and CD45), and endothelial marker (CD31) (figure 3.3) 58 Figure 3-3 Flow cytometry analysis of the stem cells surface...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 3
... labeled cells (the average amount of the iron nanoparticles per cell was assumed as 10ng /cell; e.g 1, 10, 1000 µg iron nanoparticles were mixed with agarose as representative of 100, 1000, 100000 cells) ... the MSCs did not affect the proliferation rate of the cells over time 3.4.6 Differentiation potential of labeled MSC MSCs are multipotent stem cells, which can differentiate to different lineage ... pig’s knee explants with blank and scaffold only defect (A), 100 cells simulation (B), 1000 cells simulation (C), and 100,000 cells simulation (D) Left panel of each image is 3D-FSE sequence...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 4
... labeled cell localization data (Arrowheads show the distribution of the labeled cells in the knee joint) MSCs were distributed in different places of the knee (figure 3.14) The labeled cells migrated ... site and arrows demonstrate the labeled stem cells) 72 Figure 3-16 MRI of the distribution of the labeled MSCs in the mini-pig knee joint The labeled cells migrated to the para-patellar fat ... (calculated based on Wakitani scoring system at table 3.1) were compared between the scaffold only and scaffold plus MSCs groups and showed a significant difference in the cell morphology (hyaline cartilage...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 6
... (189) This difference could be the result of lower loading of the cells (5 pg iron per cell) by Heymer et al., which is similar to our cells iron content labeled with lower labeling concentration ... chondrogenic differentiation Farrell et al (204) showed that SPIO labeled cells negatively affected the osteogenic differentiation of the cells in vivo In contrast, our study showed that iron labeling did ... MSCs is indeed feasible in vivo Cells migration and localization into different site of the inflammation was observed in the knee joint over time A majority of the cells moved to the surgery sites...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 7
... a limited capacity of self-repair Cell based therapy is one promising approach in the treatment of damaged cartilage Bone marrow (BM) derived mesenchymal stem cell (MSC) is a good candidate because ... mosaicplasty, and cell based therapy (autologous chondrocyte implantation, or matrix associated cell implantation)(29) Recently, cell based therapy such as mesenchymal stem cells (MSCs) become ... biochemical factors essential in mimicking physiological conditions for cells after administration Key words: Mesenchymal stem cell (MSC), cell migration, microfluidic device, cartilage, tissue culture...
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Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates
... in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal stem cells ... giant cell (MNGC), blood vessel (BV), lymphoid aggregates (LYM), bone marrow (BM) Fig 6.6: Histology of new bone formation (a) and (b) bone consists of mature bone (pink) and immature bone (cartilage) ... affinity cell- binding domains [15] Collectively, the activation of type I Col-specific integrins is said to have an osteogenic response to a bone cell line [16] and human bone marrow stem cells...
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báo cáo hóa học:" High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia" pot
... AML blasts collected from bone marrow aspirates in comparison to blasts from peripheral blood samples not differ basically This may indicate, that samples of peripheral blood with high amounts ... biological differences for both cell types In the present study, we aimed to investigate the profile of protein expression of blast populations from peripheral blood and bone marrow aspirates using a ... (85%) * 5.2 (81%) * 37.0 (87%) * B C D E F WBC = white blood cell count, * = bone marrow samples were adjusted to 1.000.000 mononuclear cells per assay Page of (page number not for citation purposes)...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... repair cells stemming from the bone marrow [14] Indeed, bone marrow has become a recognized source for progenitor cells of several cell types [15], including EC [13,16,17], epithelial cells [13,18-23], ... engrafted cells, where circulating blood cells, dead cells, cell fusion, or artifacts like autofluorescence might lead to misinterpretation Therefore, the reconstitution of lung epithelium by bone marrow ... http://respiratory-research.com/content/8/1/50 Figure Rat bone marrow- derived EPC in culture Rat bone marrow- derived EPC in culture A highly purified population of EPC was isolated from hindlimb bone marrow of male Sprague-Dawley...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
... investigate possible mechanism involved in the release of pyrogenic cytokines from peripheral blood mononuclear cells (PBMC) and the pyrogenic response in rabbits Results Febrile response to the ... been studied In T cells, SEB or SEC interacts with specific variable b (Vb) elements on a ⁄ b T cell receptor, such as Vb 3, 12, 13.2, 14, 15, 17 and 20 [38,39] Stimulation of a T cell receptor results ... superoxide also mediate signaling transduction [22] In nonphagocytic cells, such as fibroblasts, endothelial cells, vascular smooth muscle cells, cardiac myocytes and thyroid tissue, ROS are produced...
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Báo cáo khoa học: Effect of 5-lipoxygenase inhibitor MK591 on early molecular and signaling events induced by staphylococcal enterotoxin B in human peripheral blood mononuclear cells doc
... signaling systems efficiently utilized by cells to transmit and integrate a plethora of intracellular activities [6] Although the well regulated signal transduction is crucial for typical cell behavior, ... receptor in endothelial cells [16] Superantigens are known to activate large families of T cells based on expression of the Vb chain of the T cell receptor, which in turn increases cell proliferation ... directly proportional to cell proliferation, was quantitated as described in the Experimental procedures Table Effectiveness of MK591 on SEB stimulated cellular activities Each cell type was treated...
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báo cáo hóa học:" Combined intermittent hypoxia and surface muscle electrostimulation as a method to increase peripheral blood progenitor cell concentration" pot
... significant differences were found when bone marrow cells or SCs from peripheral blood were compared [18], nor when the comparison was made between bone marrow cells and adipose tissue-derived SCs ... Transendocardial, autologous bone marrow cell transplantation for severe, chronic ischemic heart failure Circulation 2003, 107:2294-2302 Cashen AF, Lazarus HM, Devine SM: Mobilizing stem cells from normal ... Journal of Translational Medicine 2009, 7:91 Background Stem cells (SCs) are primitive cells with the potential to differentiate into mature cells [1] An increase in SCs is observed after various...
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báo cáo hóa học:" yuDetecting the percent of peripheral blood mononuclear cells displaying p-STAT-3 in malignant glioma patients" pot
... substances are capable of reaching the immune system and peripheral blood stream Therefore, we hypothesized that the percent of peripheral blood mononuclear cells (PBMCs) displaying p-STAT-3 may be ... in immune cells in the cervical lymph nodes, which then are detected in the peripheral circulation Therefore, we measured p-STAT-3 in glioma patients' peripheral blood mononuclear cells (PBMCs) ... be increased in the peripheral blood in two ways: (1) a tumor with p-STAT-3 would subsequently induce pSTAT-3 in tumor-associated immune cells, which would then reenter systemic circulation or...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... method to induce human MDSC from healthy donor peripheral blood mononuclear cells (PBMC) by co-culture with human solid tumor cell lines Suppressor cells generated by this method demonstrate features ... myeloid-derived suppressor cells; (NOX2): NADPH oxidase; (NFκB): nuclear factor kappa B; (PBMC): peripheral blood mononuclear cells; (PGE): prostaglandin E2; (Treg): Page 19 of 20 regulatory T cells; (STAT3): ... Pan PY: Development and function of myeloid-derived suppressor cells generated from mouse embryonic and hematopoietic stem cells Stem Cells 2010, 28:620-632 Poschke I, Mougiakakos D, Hansson J,...
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báo cáo hóa học: " Vascular endothelial growth factor-A and chemokine ligand (CCL2) genes are upregulated in peripheral blood mononuclear cells in Indian amyotrophic lateral sclerosis patients" pot
... chemokine ligand-2; PBMCs, peripheral blood mononuclear cells and differentiation of VEGF receptor (VEGFR1), VEGFR2 and chemokine receptor (CCR2) expressing adult neural progenitor cell into neuronal ... sclerosis; VEGF-A, vascular endothelial growth factor-A; CCL2, chemokine ligand-2; PBMCs, peripheral blood mononuclear cells levels did not vary between definite, probable and possible ALS cases (Figure ... Indian ALS patients [9] We now measured the mRNA expression of VEGF-A and CCL2 in peripheral blood mononuclear cells (PBMCs) of these patients Subjects and methods 50 patients, born in North India...
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báo cáo hóa học:"Transplantation of selected or transgenic blood stem cells – a future treatment for HIV/AIDS?" pot
... problem is alleviated by the worldwide cooperation of stem cell registries in the Bone Marrow Donors Worldwide and European Marrow Donor Information System networks, which are currently in the process ... HIV resistance transgenes in stem cells Another approach to the provision of HIV-resistant blood cells is the introduction of resistance-conferring genes into stem cells before transplantation ... fact that cord stem cell transplantation in adults currently requires more than one cord blood unit Donor recruitment A major issue concerning the supply of CCR5-negative stem cells is the donor...
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