block copolymer morphologies as revealed by afm and tem

... thermophilus RNase HI (Tt-RNase HI) Gly39 and Gly97 are conserved as Gly41 and Gly100 in Tt-RNase HI, respectively Val76 is conserved as Val74 in Ec-RNase HI Asn90 is conserved as Gln96 in RNase HI from ... R97G-RNase HI, D136H-RNase HI, 5·-RNase HI and 6·-RNase HI were constructed by sitedirected mutagenesis using PCR as described previously [33] Plasmid pET500M or pET500M4x was used as template ... such as Asp12, Glu50, Asp72 and Glu133, are clustered in the vicinity of Asp136 It has been reported that the corresponding mutation (Asp134 fi His) stabilizes Ec-RNase HI by 7.0 °C in Tm as a...

Ngày tải lên: 18/02/2014, 13:20

...  43 000 This band appeared as a degradation product during freezing and thawing of the protein that was stored at )30 °C As revealed by immunoblotting, all the mutants, as well as the wildtype, ... synthesis by chitinase A Protein expression and hydrolytic activity of the wild-type chitinase A and mutants B C Fig SDS ⁄ PAGE analysis of the recombinant chitinase A and mutants Purified chitinases ... hydrolysis, as a function of time, revealed some differences between the nonmutated and mutated enzymes As with native chitinase A, (GlcNAc)2 did not act as a substrate and (GlcNAc)3 was a poor...

Ngày tải lên: 20/02/2014, 01:20

... shift slowly to blue against a temperature increase below 293 K, and the shift is faster and shows linear temperature dependence above 293 K In addition, the decrease in fluorescence inten- Fig ... used as a tem plate (Fig 5A, upper right) and 3.62 and 5.86 A to O10b and O10a, respectively, when the LH2 of Rps acidophila was used (Fig 5B, upper right) In the LH2s of Rs molischianum and ... of Ca2+-binding proteins, and is also known as the EF hand [16] Proteins containing the EF hand are divided into two classes according to their functions: signaling and buffering ⁄ transport...

Ngày tải lên: 30/03/2014, 02:20

... contra-lateral channel by 3.62 dB and shifting its onset by 6.5 μs The impression of a 45° lateralisation was created by attenuating the contralateral channel by 2.8 dB and delaying its onset by μs Stimuli ... sound lateralisation was created by intensity and phase differences between the left and right channel The impression of a 90° lateralisation to either direction was created by attenuating the contra-lateral ... Headphones were put on and the 30 - experiment was started after impedance measurement After the EEG experiment intelligence was assessed by the Coloured Progressive Matrices (CPM) [41] Task Participants...

Ngày tải lên: 11/08/2014, 16:22

... pyrophosphatase (Sigma), 0.7 nmol template DNA, and 52 nm T7 RNA polymerase prepared from the recombinant pAR1219 expression plasmid [58] Incubation was performed for h at 37 °C, and was followed by purification ... ensiformis 3¢-terminal 55-base sequence The remaining 5¢ region was assembled taking into account conserved D-loop bases and the base-pairing requirement of the D-loop and acceptor stems, while bearing ... sulphate was converted to l-canaline by arginase treatment, essentially as described previously [61] The arginase required for this was obtained as a crude extract from the leaves of Canavalia brasiliensis...

Ngày tải lên: 18/02/2014, 13:20

... Caspases-2, 8, 9, 10 and 12 function upstream within the apoptotic signaling pathways and are termed initiator caspases (group II) Caspases-3, 6, and 14 are activated by initiator caspases and ... subsite of caspase-7 ⁄ IEPD (F) Comparison of P4 Ile in the S4 subsites of caspase-7 and caspase-8 ⁄ IETD The caspase-7 residues are colored by atom type, whereas those of caspase-8 are shown ... in procaspase-7 and procaspase-3 have the sequences DSVD and ESMD, respectively, which implies that P3 Ser is physiologically acceptable by initiator caspases In some cells, caspase-3 was shown...

Ngày tải lên: 18/02/2014, 16:20

... ugly, and brutal ...

Ngày tải lên: 21/03/2014, 21:54

... AF506977) and the C gigas MT1 gene [22], as shown in Fig 2B The CvMT-I gene was found to have the same intron phase (1,1) as the CvMTA gene, whereas the C gigas MT1 gene had a (1,2) intron phase The ... trifluoroacetic acid) and the mass was determined by MALDI-TOF MS (Voyager-DE STR BioSpectrometry Workstation; Applied Biosystems) Cysteine content was determined by subtracting the mass of the native ... Briefly, the sample was deoxygenated with N2 gas and lL of 100 mM dithiothreitol was added before the sample was incubated at 45 °C for 60 min, after which lL of 0.2 M IAA was added and incubated at...

Ngày tải lên: 30/03/2014, 13:20

... emerged as a powerful and sensitive tool to analyze white matter disease Specifically, DTI has been utilized to assess axonal damage and demyelination in vivo in both MS and in animal models such as ... scores and SMI-32 staining was analyzed via Two-way ANOVA Highest severity score by disease phase and RA, λ⊥, λ|| map histograms were analyzed by oneway ANOVA All analyses were followed by Bonferroni ... permeabilized and blocked in 0.1% Triton X-100 and 10% goat serum for 60 minutes at room temperature Primary antibody was incubated overnight at 4° Slides were washed in C PBS followed by incubation...

Ngày tải lên: 19/06/2014, 22:20

... provided by only considering mental health or psychopathological aspects, at least when measured as parent reported emotional and behavioural problems on the CBCL The child’s QoL as measured by the ... comprised of positive and negative affect as an emotional appraisal of health and life circumstances, as well as an emotional state that is determined by inter-personal aspects, temperament, etc Although ... integrated system of multiinformant assessment of children and adolescents that has been used extensively in research and clinical practice Parents are asked to report on children’s emotional and behavioural...

Ngày tải lên: 20/06/2014, 15:20

... herbaceous root biomass (RHT), total Scots pine root biomass (RPT), stand root biomass including pine and herbs (RCT), herbaceous aerial biomass (HAB), and the VAST3 competition index (VAS) Variables ... 19] 2.3 Assessment of competition aboveand belowground Competition belowground was assessed by estimating the fine-root biomass and morphology of competitor plants (i.e Pinus sylvestris and herbaceous ... overstory, depending on pine number, size and distance Aboveground competition by pines was assessed by measuring the distance, the diameter at breast height (dbh) and the height of all surrounding pines...

Ngày tải lên: 08/08/2014, 00:21

... therapeutic and side effects, compared to the correlation between therapeutic effects as assessed by teachers and side effects as assessed by parents Methods Subjects Children between the ages of and ... consent was provided by the parents, and all children verbally agreed to participate in the trial with MPH Approval for the study was granted by the Research Ethics Board of Douglas Hospital Assessments ... study was a double-blind, placebo-controlled, crossover, randomized trial Baseline assessments were made following a two-week washout period and week before the trial began, while the child was not...

Ngày tải lên: 11/08/2014, 15:22

... patients, EASE based on the Gene Ontology (GO) database, which can classify large gene lists into functionally related gene groups and rank their importance, was performed EASE classified the ... (RA) is a systemic autoimmune disease characterized by chronic synovitis that is often pathogenic and destructive to articular cartilage and bone To understand the complex pathogenesis and heterogeneous ... GO systems: biological process, cellular component, and molecular function Up-regulated and down-regulated genes for the GO system biological process based on EASE are shown in Tables and 3,...

Ngày tải lên: 12/08/2014, 17:21

... Da MUP by gel electrophoresis, tandem mass spectrometry and peptide mass fingerprinting (PMF) Identification of 18,956 Da MUP by gel electrophoresis, tandem mass spectrometry and peptide mass fingerprinting ... electrophoresis as a low mobility band, and on SDS-PAGE as a higher mass band (Figure 6a) In both instances, the bands could be excised and digested with trypsin or endopeptidase LysC, generating ... previously been described as expressing non-urinary MUPs Transcription of the B6 gene 1/S7 gene has been described in lachrymal and parotid gland tissue [23], and the set of cDNAs and ESTs corresponding...

Ngày tải lên: 14/08/2014, 08:21

... the manuscript and A Sandoz for drawing the map of Albania This study was funded by a grant awarded by the French Embassy in Tirana, Albania, to Dr AJ Crivelli and Dr M Lutz, and by the Ministry ... species and one genus: S ohridanus (5), S letnica (5) and S peristericus (20), and Salmo sp (57) While the first two species are easily recognizable on the basis of both their distinct phenotype and ... Ohrid), the classification of S peristericus was based upon its very restricted distribution (see [18]) The other specimens were phenotypically indefinable and were therefore assigned as Salmo sp...

Ngày tải lên: 14/08/2014, 13:21

... haplotype was accounted for as the haplotype frequency The NJ tree was constructed as described above Median networks were constructed using an algorithm for speedy construction by hand [1] They ... supported by relatively high bootstrap values One of them consisted of the Slavina, Dubovina and X haplotypes and the other one of the G, F and Trompeta haplotypes (Fig 1) On the contrary, the C2 and ... of artificial selection However, an alternative model was also suggested by the same author [3]: this is based on the assumption that there was a geographical cline in the populations of 646 T Kavar...

Ngày tải lên: 14/08/2014, 13:21

... explained by some students that the report assignment was uninspiring and they generally could not reach the required depth, or it was neglected by teachers because much more in class time, effort and ... and teachers' perception in this regard was gauged through questions for the presentation assignment and questions for the reflection assignment And the result was positive The presentation assignment ... Himmel, J., 2007) as shown above Chapter 2: Literature Review and Practical Basis Its neighbor, the adjunct model, can be refuted as being noted by Davies (2003) as "[its] emphasis is placed on...

Ngày tải lên: 30/03/2015, 14:00

... approach as well as specific methods used in data collection and data analysis Chapter is Findings Analysis and Discussion The findings from the questionnaire and interview are presented, described and ... Misunderstanding in intercultural communication: Interactions in English as a lingua franca and the myth of mutual intelligibility In: Gnutzmann, C (Ed.) Teaching and Learning English as a Global ... Language Teachers and English as an International Language TESL Reporter 26/1, 29-34 Gnutzman, C (2000) Lingua franca In Byram, M (Ed.) The Routledge Encyclopedia of Language teaching and Learning...

Ngày tải lên: 04/08/2015, 09:39

... Frequencies and Percentages for Each Item Relative to Instrument Question 77 Means and Standard Deviations for Each Item Relative to Instrument Question 79 Means and Standard Deviations for Each Item ... 80 Means and Standard Deviations for Each Item Relative to Instrument Question 10 81 10 Means and Standard Deviations for Each Item Relative to Instrument Question 11 82 11 Means and Standard Deviations ... Society (TCPAS) Established in 1903, the ICPAS is the premier association for CPAs in Illinois The ICPAS is a professional organization of CPAs which, as part o f its activities, assists the...

Ngày tải lên: 30/09/2015, 13:41