biology and potential role in tumor immunity

... different roles in angiogenesis: Ang-1 and Ang-4 are agonist ligands for Tie2 and induce tyrosin phosphorylation of Tie2, while Ang-2 and Ang-3 are antagonist ligands They bind to Tie2 without inducing ... Ang-2 was mainly expressed in tumor stroma and had similar expression pattern in malignant and benign tumors (Figure 1C, D) Maspin expression was predominantly located in the cytoplasma and occasionally ... papillary cystadenocarcinoma; D: Ang-2 expression in ovarian borderline mucinous cystadenoma; E: Maspin expression in mucinous cystadenocarcinoma; F: Maspin expression in mucinous cystadenoma The...

Ngày tải lên: 10/08/2014, 10:21

... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... molecular chaperones in the folding of oxidized proteins Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein J Biol Chem 2001, 276:21337-21342 ... proinflammatory cytokines play a critical role in the initiation and progression of sepsis syndrome and that TNF-α, interleukin (IL)-1β, and IL-6 are important mediators of hemodynamic, metabolic, and immunologic...

Ngày tải lên: 13/08/2014, 11:22

... conditions [62] Interestingly, we observed that the insulin mRNA PTB binding site has an overlapping consensus CSD binding site PTB, to date, has only been implicated in stability of insulin and VEGF ... proteins may also play a role in post-transcriptional regulation of VEGF expression in the cytoplasm, in conjunction with another singlestrand RNA/DNA binding protein, PTB Conserved CSD/PTB binding ... (3¢) protein binding sites are underlined Both sequences are required for cytoplasmic CSD/PTB complex formation and recombinant CSD protein binding (B) A comparison of CSD protein RNA binding sites...

Ngày tải lên: 19/02/2014, 12:20

... than in OA and AS synovium CD147 was expressed predominantly on the macrophage-like cells and fibroblast-like synovial cells in the lining and sublining layers (Fig 2) MMP-1 was expressed predominantly ... CD147), matrix metalloproteinase-1 (RA MMP-1), tissue inhibitor of metalloproteinases-1 (RA TIMP-1) and blank contrast (RA blank) in the lining and sublining layers in RA synovium (× 400) The ... (green) and CD147+ (red) cells, and the co-expression of CD68+CD147+ cells (yellow) in the lining and sublining layers of rheumatoid arthritis synovium is apparent Immunohistochemistry staining of...

Ngày tải lên: 09/08/2014, 06:23

... the in vivo expression of 15-LOX-1 and -2 proteins in OA cartilage 13-HODE and 15-HETE inhibited IL-1β-induced MMP-1 and MMP-13 expression in chondrocytes To examine the effects of 15-LOX-1 and ... IL-1β-induced MMP-1 and MMP-13 expression by interfering with key signalling pathways In this context, 15LOX metabolites were shown to inhibit protein kinase C activity and translocation [20,49], and ... washed and incubated for an additional 24 hours in DMEM containing 0.5% FCS, before stimulation with either IL-1β alone or in combination with 13-HODE or 15-HETE 13-HODE and 15HETE, supplied in ethanol...

Ngày tải lên: 09/08/2014, 14:20

... pancreatic and prostate tumors Retinoblastoma protein, myosin regulatory light chain-interacting protein and retinoblastoma-binding protein 1-like miR-155 Overexpressed in CLL, B-cell, Hodgkin’s and ... Adenosine-to-Inosine editing of miR376 cluster in gliomas 63 Introduction and aims 63 3.2 Editing analysis of primary miRNAs in gliomas 3.3 Editing analysis of primary miRNAs in glioma cell lines and ... adenosine-uracil pair to an inosine-cytidine pair (Figure 1.5B) 35 A B Figure 1.5 Adenosine deamination to inosine by ADAR A Hydrolytic deamination converts adenosine to inosine B Base-pairing...

Ngày tải lên: 10/09/2015, 08:28

... pro-apoptotic activity of Bax and Bak by preventing the binding of these proteins to anti-apoptotic Bcl-2, suggesting that these pro-apoptotic proteins kill by binding and inhibiting their anti-apoptotic ... leading to cytochrome c release (Mihara, Erster et al 2003) Interestingly, this was achieved in the absence of a BH3 domain in p53, instead p53 binds to Bcl-2 using its proline-rich domain (Mihara, ... COX Va and COX Vb as cancer markers: 32 Interestingly, recent studies have demonstrated up-regulation and increased involvement of COX Va and Vb in a variety of cancers Autocrine gastrins-induced...

Ngày tải lên: 14/09/2015, 08:42

... C-terminal epitope of Vgf Unoccupied binding sites on the plates were blocked by incubation with casein Samples and standards were applied in duplicate and incubated overnight at 4°C Following ... tissue staining intensities influenced the measured values Average value density measurements from individual Vgf immunoreactive dorsal spinal cord neurons, reflecting immunostaining intensity, ... delimiting the cellular area of interest free hand, using predetermined criteria to define the region of interest The immune intensity of the cellular Vgf encompassing the L3-L5 regions of spinal...

Ngày tải lên: 03/11/2012, 10:52

... very-KIND-MAP2 interaction v-KIND KIND2 KIND2-1 KIND2-2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... the structural and functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across ... deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 domain (B) KIND2 domain anchors v-KIND to dendrites Flag-tagged v-KIND, DKIND1, DKIND2, DKIND1 + 2, DRasN, DGEF, KIND1 or KIND2 together with...

Ngày tải lên: 14/02/2014, 19:20

... wild-type occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon played a major role in the induction of mitochondria-mediated apoptosis and the ... occludin in apoptosis and invasion A C B D E F Fig Identification of a human occludin splice variant in liver cell lines (A) Occludin protein expression was examined with an antibody to the N-terminus ... cells In Chang cells, stained occludin was observed in the cytoplasmic region Additionally, in OccWT-overexpressing Hep3B and Huh7 cells, regions stained with antibody to hemagglutinin (HA), indicating...

Ngày tải lên: 18/02/2014, 18:20

... copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain swapping and forms ... C-terminus (box in Fig 1) at sites: 92, 75, 66 and 58 As histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although ... binding on the secondary structure was determined using CD in the far UV To prepare proteins without Cu2+, part of each protein 4252 Fig Comparison of copper binding by stefin A and stefin B using...

Ngày tải lên: 19/02/2014, 06:20

... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important role in leucine catabolism AUH was overproduced in E coli and characterized by measuring ... (c.719C>T) in exon and an insertion mutation c.613–614insA This insertion causes a frameshift that starts at Met205 and leads to the introduction of a stop codon after four amino acids The intra-assay ... Characterisation and mitochondrial localisation of AUH, an AU-specific RNA-binding enoyl-CoA hydratase Gene 228, 85–91 Nakagawa J & Moroni C (1997) A 20-amino-acid autonomous RNA-binding domain contained in...

Ngày tải lên: 19/02/2014, 07:20

... opaque with age and contain many inclusion bodies reactive with antibody to aB-crystallin, but not to b- and c-crystallin, suggesting an important role for aA-crystallin in maintaining lens transparency ... aB-Crystallin R120 corresponds to aA-crystallin R116 and both are conserved a-crystallin domain arginines R120G aB-crystallin permits intermediate filament self association in vitro, although binding ... aAcrystallin requires this domain [66] Attachment of R116C aA-crystallin to Hsp27 and aB-crystallin increases in comparison to wild type, while binding to cC-crystallin and bB2-crystallin decreases...

Ngày tải lên: 19/02/2014, 18:20

... (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations ... were removed and minced with a surgical knife The minced tissue was wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation ... were incubated at 37 °C in a humidified 5% CO2 incubator in the absence (control) and presence of increasing concentrations of inhibitors for 10 days, and the number of molting larvae was determined...

Ngày tải lên: 20/02/2014, 11:20

... Beyond initial training, there is a need for retraining, on-the-job training, continuing education, and advanced training in management Training in problem solving, supervision, management, and ... Challenges and Potential Solutions in India: Summary of a Joint Workshop by the Institute of Medicine, the Indian Nation DRUG-RESISTANT TUBERCULOSIS IN INDIA XDR TB has been reported in India However, ... (isoniazid and rifampicin), as well as any fluoroquinolone (levofloxacin, moxifloxacin, or ofloxacin) and at least one second-line injectable drug (kanamycin, amikacin, or capreomycin) Totally...

Ngày tải lên: 05/03/2014, 11:20

... Oncogenes and Related Genes Shanghai Cancer Institute, Shanghai, China Smith RK, Carroll PM, Allard JD & Simon MA (2000) MASK, a large ankyrin repeat and KH domain-containing protein involved in Drosophila ... variant A Fig Interaction of Vpr and VBARP proteins in vitro and in vivo (A) In vitro translated 35S-labeled VBARP-His isoforms were incubated with Vpr or Nef and immunoprecipitated using a-His (lanes ... ankyrin repeat proteins, using the consensus established by Kohl et al [10] and Mosavi et al [11], which contains ankyrin repeats from over 4000 proteins, indicated that the ankyrin repeat domains...

Ngày tải lên: 07/03/2014, 21:20

... Printing Office, Washington, D.C., 2004 SOLAR AND SPACE PHYSICS AND ITS ROLE IN SPACE EXPLORATION the fundamental role of solar and space physics research both in scientific exploration and in ... public, and the scientific and engineering communities The Council is administered jointly by both Academies and the Institute of Medicine Dr Bruce M Alberts and Dr Wm A Wulf are chair and vice ... modeling can also be a valuable tool for mission planning Insights obtained from theory and modeling studies provide a conceptual framework for organizing and understanding measurements and observations,...

Ngày tải lên: 14/03/2014, 10:20

... River - drainage system in Hanoi City The four rivers are playing a very important role in water drainage in Hanoi inner The lengths and basin areas of these rivers are shown in the following table ... biodegradable and tend to accumulate in the sediments of waterways in association with organic and inorganic matter in the sediments [8] In the present study, we examined the nutritive value and potential ... by using wastewater and river water for irrigation (kg ha-1) Two horizontal lines show the nutrition demand for N, P, K of rice and maize H and L indicated the demand supplying of water being...

Ngày tải lên: 22/03/2014, 12:20

... and 20 and in Recycling (code 370) over 1996-2000 in county 19 For the corresponding years, there are no enterprises registered in the corresponding industries and counties, and submitting financial ... are found in the following industries: Agriculture and forestry (code 111) in 1990-1992, Raising of fish (code 051) in 1988-1993, Fishery (code 052) in 1988-1993, Publishing, printing and reproduction ... enterprises with debt in financial institutions in industry Transport via pipelines (code 603) in 1997 and in industry Private households with employed persons (code 950) in 1996, 1998 and 2000 Enterprises...

Ngày tải lên: 22/03/2014, 20:21

... also contain a carboxy-terminal extension containing T-box and A-box motifs [124] In each zinc finger, four invariant cysteine residues coordinate tetrahedrically to a zinc ion, and both zinc fingers ... overlapping ligand-binding pockets The difference of the binding pocket is easy to understand by looking at the superimposition between PonA and BYI06830 shown in Fig Ligand-binding affinity Typically, ... for retaining the ligand-binding activity They found two high-affinity binding sites (Kd1 = 0.24 nm and Kd2 = 3.9 nm) The removal of GST had no effect on PonA binding, but altered DNA binding The...

Ngày tải lên: 23/03/2014, 04:20