biodiversity in the algal community

... representative NDH-2 protein sequences from the four different families (Fig 2) revealed high conservation in two domains showing most of the criteria for dinucleotide binding, including a bab fold and ... an antibody raised against the recombinant protein, AtuNDH-2 was detected in protein extracts from the complemented ANN0222 strain (Fig 6C) Most of the protein was present in membrane fractions, ... domain enriched in aromatic residues located between its two cofactor binding sites This domain has been proposed to be involved in the interaction with hydrophobic quinones of respiratory chains...

Ngày tải lên: 19/02/2014, 06:20

... significantly increased binding of tau to Fyn-SH2, thus suggesting that the interaction between tau and Fyn-SH2 is enhanced by increased tyrosine phosphorylation in cells To determine whether the tyrosine ... interacting proteins Seven such motifs are present in the proline-rich domain of the longest isoform of tau in the human central nervous system (CNS) Two distinct PXXP motifs in tau, residing within residues ... tyrosines present on tau [17–19], and tau also interacts with Fyn via its Src homology (SH)3 domain [20,21] The SH3 domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting...

Ngày tải lên: 14/02/2014, 14:20

... was cleared for printing in April 2003 During the study, the Joint Program Office–Biological Defense (JPOBD) was responsible for the acquisition of biological agents In the final stages of report ... personnel INDs, however, are far more difficult to use: They may be used under all the restrictions of IND use, including informed The interviews on which the report is based were conducted in ... pharmaceutical industry revenues For drugs and vaccines intended mainly for military use, the market is simply too small to interest private-sector investment in bringing such products through FDA licensing...

Ngày tải lên: 17/02/2014, 11:20

... HAI-1 The activity of HGFA is inhibited by naturally occurring protein inhibitors belonging to different classes, such as the Kunitz domain inhibitors HAI-1 and HAI2, and the serpin protein C inhibitor ... However, owing to the tight association of the cleavage product, the peptide bond is more rapidly resynthesized, so that the intact form of the Kunitz domain inhibitor predominates in the crystal ... According to this model, the intact KD1 peptide bond in the KD1–HGFA structure thus reflects the capture of the dominant form, owing to the more favorable rate of peptide bond resynthesis during...

Ngày tải lên: 15/03/2014, 11:20

... of integrins requires talin binding to the cytoplasmic tail of b integrin [13] Talin is an essential integrin-activating protein that connects the cytoplasmic tail of b integrins to the actin ... that the PTB-like F3 subdomain of the FERM is the C-terminal counterpart As such, in the dormant form of moesin, the F3 subdomain is masked by the N-terminal extended actin binding tail domain ... four-point-one ⁄ ezrin ⁄ radixin ⁄ moesin) domains FERM domains localize proteins to the plasma membrane, where they can interact with phosphoinositides and membrane proteins [21] The FERM domain...

Ngày tải lên: 16/03/2014, 05:20

... in- frame insertions resulting in the insertion of five amino acids into the Cdc37 protein Stop codons were inserted by the five codon insertion in four mutants, each adding only 1–3 extra amino acids ... Surprisingly, we discovered that expression of the N-terminal domain alone can sustain cellular viability These truncated proteins not contain the postulated Hsp90-binding domain, suggesting that binding ... conserved The N-terminal domain of Cdc37 is the most highly conserved region and is involved in client interactions [8] In the N-terminal 40 amino acids there is 80% identity between the S pombe...

Ngày tải lên: 16/03/2014, 22:20

... curve fitting was then applied within the original spectra after the subtraction of baselines resulting from neighbouring bands Similar to the deconvolution technique, the bandshapes of the single ... De Kruijff, B (1998) An investigation into the lipid interactions of peptides corresponding to the C-terminal anchoring domains of Escherichia coli penicillinbinding proteins 4, and Biochim Biophys ... with these results, hydropathy plot analysis of the GTAMRILGGVI sequence showed a progressive increase in hydrophobicity in moving from the N-terminus to the C-terminus (Fig 2), suggesting the...

Ngày tải lên: 17/03/2014, 10:20

... on the surface of PfFabG Whereas the consequences of mutating the corresponding residues in E coli FabG involved in the binding of E coli ACP have been investigated earlier, the number of binding ... 1.10 lm)1) in the presence of NADPH, and the number of binding sites increased from one to two, whereas in all PfFabG mutants examined except R187K, the number of binding sites remained the same, ... Table Binding constants (Ka) for interaction of wild-type PfACP with mutant PfFabG, in the absence and in the presence of reduced cofactor NADPH at 20 °C, obtained using the changes in protein fluorescence...

Ngày tải lên: 23/03/2014, 10:20

... protein [16], the glutamine synthetase involved in the synthesis of poly-lglutamate–glutamine polymers [17], serine ⁄ threonine protein kinases [18–20] and other virulence factors [21,22], points ... decaprenyl-phospho-arabinose into the arabinogalactan and (lipo)arabinomannan of the mycobacterial cell wall at the level of the Emb proteins – the putative arabinan synthases Specific mutations in the Emb proteins ... block the synthesis of decaprenyl-phospho-arabinose, it interferes with the utilization of the arabinose donor by inhibiting either arabinosyltransferase activity or the formation of an arabinose...

Ngày tải lên: 30/03/2014, 04:20

... with the model of BiP binding transiently in the ER to Ig chains during protein folding and processing The immunoblot results matched those found by Northern blot, in that most BiP protein was ... proteins [9,49] and in folding and assembly of immunoglobulin chains in cells that are highly active in terms of recombinant protein production and secretion The relative decrease in BiP mRNA in ... of DNA encoding heavy chain and with increasing amounts of light-chain encoding DNA, then immunoprecipitated the immunoglobulin chain (Fig 5) The results show that when heavy chain (c) expression...

Ngày tải lên: 31/03/2014, 08:20

... the case of the role of avb3 and avb5 integrins in promoting angiogenesis, in which antibody blockade suggested key roles for these integrins in angiogenesis [31,32], but mice lacking these integrins ... vitronectin are not affected by the absence of avb3, avb5 or both these integrins Figure Evaluating the role of av integrins in mediating microglial adhesion to vitronectin A Adhesion to vitronectin ... defects in adhesion to vitronectin, or in the subsequent activation responses This is in stark contrast to our finding with brain endothelial cells, where absence of the avb3 integrin leaves the...

Ngày tải lên: 19/06/2014, 22:20

... example, gene × smoking) in the conditional logistic regression models The significance of the interaction was determined using the Wald χ2 test of the interaction variable In the combined NHS-NHSII ... between the presence of the PTPN22 polymorphism and smoking in determining RA risk Their gene-smoking interaction analyses used data from the Swedish Environmental Investigations in RA incident ... did not affect risk estimates either and these were not included in the final models As is evident comparing the results of a model taking only the matching factors into account, adjustment for...

Ngày tải lên: 09/08/2014, 10:23

... assessed by monitoring the reproducibility of the G-band peak intensity at ~1590 cm-1 since it is the most prominent Raman peak indicative of intrinsic SWNT features [109] In brief, the relative standard ... Since >99% of the Mo and Co present in the as-received SWNT-containing powder was not detected in the DM-SWNT dispersions (relative to oxidized metal levels from the TGA of the SWNT-containing ... was effective in removing metals from the raw, asreceived SWNT-containing powder Herein, we present the characterizations of an as-received CoMoCAT SWNTcontaining powder using thermal gravimetric...

Ngày tải lên: 11/08/2014, 00:22

... grain yield-involved, genes involved in grain yield; GDMC-interaction, the grain yield-involved genes which negatively interacted with grain dry matter content; GDMC-involved, genes involved in ... Grain yield-involved, genes involved in grain yield; GDMC-interaction, the grain yield-involved genes which negatively interacted with grain dry matter content; GDMC-involved, genes involved in ... On the one hand, it detects the most relevant genes in Step using the stringent significance level of p < 0.0001 On the other hand, it also includes further important genes with the less stringent...

Ngày tải lên: 12/08/2014, 03:21

... HIV-1 inhibits the function of pDCs to allow maintenance of the virus within the host DC-mediated HIV-1 trans infection The most interesting aspect of HIV-1 infection in DCs is the ability of the ... harbour the virus in vivo during antiretroviral therapy [112], suggesting that it is the DCs in the lymph nodes that act as the long-term reservoir This thinking is further supported by other studies ... Tat protein, is inhibited [94] Tat binds to the 5' long terminal repeat sequence of the integrated genome in complex with two host proteins, cyclin T1 (CycT1) and cyclin-dependent kinase (CDK9),...

Ngày tải lên: 12/08/2014, 23:21

... receptor binding activity and was secreted from the cell [11] Presumably the absence of TM sequences in the Env precursor protein prevented the normal folding of the CasBrE receptor binding domain (RBD) ... results in the restoration of CasBrE SU-mCAT-1 binding activity capable of establishing superinfection interference and facilitating ecotropic receptor-dependent virus infection The finding that ... further explored the extent to which this interaction could influence superinfection interference by binding to mCAT-1 within the NSCs and prevent infection by a second ecotropic virus, Fr57E The...

Ngày tải lên: 13/08/2014, 01:20

... and which strains are involved It is virtually impossible, therefore, to develop vaccines directed against pandemic in uenza strains in advance Vaccines against new in uenza strains only become ... treatment appears to be the only therapeutic option for postinfluenza pneumonia Further insight into the underlying mechanisms in combined viral/bacterial infection and post -in uenza pneumonia may ... following influenza can be subdivided into combined viral/bacterial (left) and post-influenza pneumonia (right) During combined viral/bacterial pneumonia, the virus, the bacteria and the host all interact...

Ngày tải lên: 13/08/2014, 20:21

... resulting in the release of intracellular material into the surrounding environment and initiating pro-inflammatory responses In certain circumstances, this can lead to a break in tolerance to intracellular ... overlapping pathways, the ‘extrinsic’ and the ‘intrinsic’ pathway The extrinsic pathway of apoptosis is initiated in response to external stimuli received by the cell (Figure 1.1) Extrinsic signals include ... to enter the joint to promote the on-going inflammatory response, resulting in the gradual clinical improvement seen on RTX treatment However, this hypothesis does not explain why the clinical...

Ngày tải lên: 22/12/2014, 19:48

... gives them a 90% chance of winning 10 points, the highest chance at the highest possible points in the game We then remind them that they can send messages to auditors about which action they ... reporting, the auditor has a 70% chance of earning 10 points and a 30% chance of earning point, while the manager has a 10% chance of earning points and a 90% chance of earnings points If the players ... Krantz (1999) theorized that the traditional findings of Support Theory would reverse in settings where individuals had low knowledge of the subject of the probability assessment Their participants’...

Ngày tải lên: 06/01/2015, 19:41