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biocompatible nanoparticle labeling of stem cells and their distribution in brain

Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages

Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages

Cao đẳng - Đại học

... of LIF in the culture of porcine IPS cells 90 6.4 Importance of doxycycline in the culture media 91 6.5 Differentiation of porcine IPS cells into cardiomyocytes 92 6.6 Use of porcine cells ... Culture of cells transitioning from fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced ... GENERATION OF PORCINE INDUCED PLURIPOTENT STEM CELLS AND THEIR DIFFERENTIATION INTO CARDIAC LINEAGES TAN WAN CHIU GRACE (B.Eng.(Hons.), NTU) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING...
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Báo cáo Y học: Fluorescent analogs of UDP-glucose and their use in characterizing substrate binding by toxin A from Clostridium difficile pdf

Báo cáo Y học: Fluorescent analogs of UDP-glucose and their use in characterizing substrate binding by toxin A from Clostridium difficile pdf

Báo cáo khoa học

... presence of saturating concentrations of the metal cofactor Together, these data are indicative of two modes of substrate binding (i.e open and closed) The closed, active form of UDP-Glc binding is ... hydrolysis of UDP-Glc in the absence of toxin Inhibition studies were performed by varying the MUG and UDP-Glc concentrations and following the kinetics of the hydrolysis reaction Synthesis of MUG, ... 7.6 and 150 mM KCl containing varying concentrations of UDP-Glc (0–145 mM) were studied The variable concentrations of UDP-Glc were obtained by starting with a stock solution containing all of...
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Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) potx

Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) potx

Báo cáo khoa học

... from a human pancreatic cell line in vitro and in vivo Mol Endocrinol 15, 476 - 483 Dzierzak, E et al (1998); Embryonic beginnings of definitive hematopoietic stem cells Ann N Y Acad Sci 872, 256 ... Identification of novel circulating human embryonic blood stem cells Blood 96, 1740 - 1747 IItskovitz - Eldor, J and et al (2000).; Differentiation of human embryonic stem cells into embryoid ... endocrine precursor cell line Mol Endocrinol 14, 814 - 822 Jones, G M., et al (2000); Human embryonic stem cells technology Semin Reprod Med 18, 219 - 223 Orlic D et al (2001); Bone marrow cells...
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Báo cáo hóa học:

Báo cáo hóa học: " Research Article New Trace Bounds for the Product of Two Matrices and Their Applications in the Algebraic Riccati Equation" potx

Hóa học - Dầu khí

... Majorization and Its Applications, vol 143 of Mathematics in Science and Engineering, Academic Press, New York, NY, USA, 1979 17 C.-L Wang, “On development of inverses of the Cauchy and Holder inequalities,” ... /n 3.1 In this section, we obtain the application in the algebraic Riccati equation of our results including 3.1 Some of our results and 3.1 cannot contain each other Theorem 3.1 If 1/p and K ... References K Kwakernaak and R Sivan, Linear Optimal Control Systems, John Wiley & Sons, New York, NY, USA, 1972 D L Kleinman and M Athans, “The design of suboptimal linear time-varying systems,” IEEE Transactions...
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báo cáo khoa học:

báo cáo khoa học: "A Review of Metallothionein Isoforms and their Role in Pathophysiology" ppsx

Báo cáo khoa học

... laboratory animals and in humans The above findings indicate that differences in the cellular availability of zinc in both insulin producing b -cells and in insulin target cells are associated ... replace insulin in mammalian cells cultured in serum-free media [18] The actions of zinc are intracellular because zinc increases the phosphorylation state of the insulin receptor, and hence, protein ... pancreatic b -cells with zinc The other is MT 1A, a member of a protein family that links zinc and redox metabolism Changes in the availability of zinc ions modulate insulin signaling and redox processes...
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Báo cáo khoa hoc:

Báo cáo khoa hoc:" A review on SNP and other types of molecular markers and their use in animal genetics" ppt

Báo cáo khoa học

... that interesting to use, due to the fact that they are of the dominant type, the RAPDs and AFLPs have a great advantage in terms of ease of use in the laboratory Indeed, fingerprint types of patterns ... available, indications of high densities of SNPs in defined regions can be found A sequencing study of fragments of the leptin and amyloid precursor protein (APP) genes in 22 diverse individuals ... to in Tables I and II are still in use and the choice of one or another can be guided by the variety of parameters indicated, mainly according to the goal of the study and the importance of the...
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Genetic polymorphisms of matrix metalloproteinases and their inhibitors in potentially malignant and malignant lesions of the head and neck potx

Genetic polymorphisms of matrix metalloproteinases and their inhibitors in potentially malignant and malignant lesions of the head and neck potx

Báo cáo khoa học

... vital role in maintaining the integrity of healthy tissues Disturbance in balance of MMPs and TIMPs is found in various pathologic conditions, including rheumatoid arthritis, periodontitis and cancer ... metalloproteinase inhibitors (MMPIs) in cancer therapy Inhibition of MMP activity in the ECM has been involved in invasion of malignant cells Shah et al reported functional degradation of ECM and therapeutic ... leading factors to development of OSCC [65] Tissue inhibitors of metalloproteinase (TIMPs) Tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring inhibitors of MMPs which inhibit...
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Báo cáo y học:

Báo cáo y học: "Indigenous utilization of termite mounds and their sustainability in a rice growing village of the central plain of Laos" pdf

Báo cáo khoa học

... surface, underground and the area surrounding the mounds including standing trees in the study area in August and November in 2007, from October to December in 2008 and March in 2009 Their species were ... sustainability of their use been evaluated The objective of this study is to evaluate the possibility of sustainable use by measuring the change in volume of selected mounds and by investigating ... al.: Indigenous utilization of termite mounds and their sustainability in a rice growing village of the central plain of Laos Journal of Ethnobiology and Ethnomedicine 2011 7:24 Submit your next...
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Báo cáo y học:

Báo cáo y học: "The role of mast cells and fibre type in ischaemia reperfusion injury of murine skeletal muscles" pot

Báo cáo khoa học

... manifestation of the reperfusion injury In order to determine the usefulness of therapies against mast cells, it is important to know the degree to which mast cells are involved in IR injury of other ... obtained by counting a minimum of 100 mast cell profiles in tongue, skin and heart as well as skeletal muscle Using 100 X magnification all mast cell profiles that fell within a grid area of 1.35 ... blue staining, mast cells were easily distinguished from other cells, by their red or purple stained cytoplasmic granules respectively In general, they were intact but varied in size, and were...
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Báo cáo y học:

Báo cáo y học: "Evolutionary history and functional implications of protein domains and their combinations in eukaryotes" pptx

Báo cáo khoa học

... (c) Changes of domain combination networks and union networks in lineages of S cerevisiae and H sapiens during evolution Bold and dashed lines indicate γ of domain combination networks and union ... eukaryotes, in terms of the preference for generation of domain combinations Results Assignment of domains and their combinations We used the domains defined in the Pfam database [19] Of 7,459 domains ... Death domain CH 08 Euk Calponin homology (CH) domain zf-RanBP 07 Euk Zn-finger in Ran binding protein and others fn2 07 Deu Fibronectin type II domain Xlink 07 Deu Extracellular link domain F5_F8_type_C...
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Synthesis of ionic liquids and their applications in capillary electrophoresis

Synthesis of ionic liquids and their applications in capillary electrophoresis

Cao đẳng - Đại học

... zone and σ T is the total spatial variance of the concentration profile of the zone (band broadening) caused during the migration 1.2.3.2.2 Band broadening Band broadening is encountered in all ... Aiping, Ms Yuan Linlin, Mr Zhan Wei, Dr Wu Yuanshen and Dr Wang Tianlin for their friendship and assistance I owe my special thanks to Dr Wei Hongping of CE Resources (Singapore) for providing ... Separation of K+ and NH4+ in human urine ……………………….…………95 Fig 4-1 Schematic representation of the IL coating procedure …………………101 Fig 4-2 Influence of alkylation time and buffer pH on the EOF of CT110...
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Bacteria of the sulphur cycle An overview of microbiology, biokinetics and their role in petroleum and mining industries

Bacteria of the sulphur cycle An overview of microbiology, biokinetics and their role in petroleum and mining industries

Tổng hợp

... St-Germain, In situ treatment of acid mine drainage by sulfate reducing bacteria in open pits: scale-up experiences, in: Proceedings of the International Land Reclamation and Mine Drainage Conference ... overview of the bacteria of sulphur cycle and the instrumental role which they play in solving some of the environmental and processing problems encountered in the mining and petroleum industries ... sulphide level in oil reservoirs or model laboratory systems subjected to this treatment 2.2 Treatment of acid-mine drainage and bioleaching of sulphide minerals Mining and mineral processing generate...
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báo cáo hóa học:

báo cáo hóa học:" Preparation of TiO2 nanotube/nanoparticle composite particles and their applications in dye-sensitized solar cells" doc

Hóa học - Dầu khí

... sensitized dye, and enhance incident light harvest, the use of TNTs in DSSCs has been explored [14-15] In the present work, the effect of combining TiO2 nanoparticles with TNTs and the resulting effect ... thickness of 12 to 15 µm was deposited onto the pretreated conducting glass using the screen printing technique and sintered again at 450°C for 15 and at 500°C for 15 in air Assembly of the DSSCs ... purely of TiO2 nanoparticles Competing interests The authors declare that they have no competing interests Authors' contributions HWC and CHL presided over and fully participated in all of the...
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Connecting brain tumor stem cells with their primary tumor and exploring glycogen synthase kinase  3ß regulation of cell fate

Connecting brain tumor stem cells with their primary tumor and exploring glycogen synthase kinase 3ß regulation of cell fate

Tổng hợp

... Phosphoinositide 3-kinase PKB Protein kinase B PLK Polo-Like Kinase PTEN Phosphatase and tensin homologue PYK2 Proline-rich tyrosine kinase Rb Retinoblastoma REMBRANDT Repository of Molecular Brain ... hence their accurate terminology as glioma stem cells or -initiating cells1 4, 16, 17 Traditionally, the similar identification of these cells in clinical specimens has followed the studies involving ... mammalian GSK3α and GSK3β with the tyrosine and serine phosphorylation sites pointed out by arrowheads (adapted from Doble and Woodgett47) Insulin stimulation of cells results in inactivation of GSK3...
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Báo cáo y học:

Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

Y học thưởng thức

... labeled in vitro An ideal labeling method in vitro must possess high sensitivity and specificity, and long half-life At present, there are a lot of labeling methods including GFP labeling, Lacz labeling, ... labeling, BrdU labeling, Y chromosome labeling and DiI labeling GFP protein is stable GFP gene can be transfected into MSCs through adenovi- 81 rus vector, resulting in stable GFP expression in ... the stem cell homing is executed in two ways: (1) Cell necrosis after trauma induces the release of a series of signal molecules, and stem cells are motivated and migrate into peripheral blood and...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot

Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot

Báo cáo khoa học

... standard Heparin binding properties of HCII treated with inhibitors of post-translational modifications In order to investigate the in uence of post-translational modifications on heparin binding, ... Effect of individual carbohydrate chains of recombinant antithrombin on heparin affinity and on the generation of glycoforms differing in heparin affinity Arch Biochem Biophys 341, 212–221 47 Whinna, ... carbohydrate chain linked to Asn169, a position in proximity to a site involved in heparin binding by HCII [47,48] Inhibition of tyrosine sulfation had an even more profound effect on the interaction...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf

Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf

Sức khỏe giới tính

... fat cells, various brain and nervous system cells, insulin-producing cells of the pancreas, bone cells, hematopoietic cells, yolk sac, endothelial cells, primitive endodermal cells, and smooth and ... of the tissue of origin Sources of adult stem cells include bone marrow, blood, the eye, brain, skeletal muscle, dental pulp, liver, skin, the lining of the gastrointestinal tract, and pancreas ... biology and biomedical applications of stem cells The workshop will examine several aspects of stem cell research, including: the biological properties of stem cells in general, the current state of...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc

Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc

Báo cáo khoa học

... distinct narrow time interval of transcription initiation [25], and regulate transactivation of several endodermand hepatocyte-specific factors, including transthyretin, albumin and tyrosine aminotransferase ... Transcriptome in hepatic induction of AT-MSCs analyzed using genmapp software version 2.1 and visualized using ConPath Navigator, a tool that enables viewing and searching of results obtained by genmapp ... apolipoprotein A-I apolipoprotein A-II apolipoprotein B apolipoprotein C-I apolipoprotein C-II apolipoprotein D apolipoprotein E apolipoprotein F apolipoprotein H fatty acid binding protein 1, liver...
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báo cáo khoa học:

báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt

Báo cáo khoa học

... resuspended nanoparticles were detected with Odyssey Infrared Imaging System Cell labeling with IRDy800CW-MNPs To investigate the labeling of cells with nanoparticles, RAW264.7 cells were seeded into ... concentrations for 24 h The cell labeling effect was evaluated by staining cells with Prussian blue and measuring the iron-loading of cells The Prussian blue staining showed that the cells were effectively ... 774/789 nm), and investigated their applicability in cell labeling and in vivo imaging Results and discussions Preparation of IRDy800CW-MNPs M-2, 3-dimercaptosuccinic acid (DMSA) has often been...
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