binding of oxygen to myoglobinand haemoglobin
Báo cáo khoa học: Binding of cGMP to the transducin-activated cGMP phosphodiesterase, PDE6, initiates a large conformational change involved in its deactivation ppt
Ngày tải lên :
06/03/2014, 00:21
... [3H]cGMP binding to solubilized PDE species is similar to binding to membranous PDE species, i.e the properties of cGMP binding to membranous PDE species may be estimated by studying cGMP binding to ... affect the affinity of Pabc for cGMP Roles of cGMP binding in PDE6 regulation Binding of cGMP to Pabc is not involved PDE activation Binding of [3H]cGMP to Pabc implies that cGMP binding may be involved ... after binding of [3H]cGMP to 100% of Pabc), dissociation of [3H]cGMP bound to Pabc was followed with or without mm unlabeled cGMP (Fig 5A) We found that the level of [3H]cGMP binding to Pabc...
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Báo cáo khoa học: The binding of IMP to Ribonuclease A pptx
Ngày tải lên :
07/03/2014, 21:20
... determinants of the IMP binding to RNase A shows that the inhibitor binds to the enzyme in a novel mode The chemical characteristics of the IMP molecule seem to impose this binding mode of IMP Subsite ... complex showed that binding of IMP in a similar manner to AMP would place the carbonyl O6 of IMP 3.1– ˚ 3.5 A away from Od1 of Asn67, Oe1 of Gln69, and Od1 of Asn71 in the B2 At the pH of the crystallization ... molecular surface of the three IMP molecules The binding of AMP to RNase A In comparison to IMP, AMP is a more potent inhibitor of RNase A Thus, Ki values of 46 lm [34] and 80 lm ([35], have been reported...
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Báo cáo khoa học: Binding of berberine to human telomeric quadruplex – spectroscopic, calorimetric and molecular modeling studies pot
Ngày tải lên :
16/03/2014, 06:20
... intensity at 522 nm due to addition of quadruplex solution was used to construct the binding isotherm (inset of Fig 3) Analysis of this isotherm following : binding stoichiometry using Eqn (7) ... number of moles of berberine bound to mol of quadruplex (Cb ⁄ Cqua), n is the number of equivalent binding sites, and Kb is the affinity of ligand for those sites [52] Data were fitted to the McGhee– ... (s) for binding of berberine to the human telomeric quadruplex Fig S2 The natural logarithm of the ratio of the binding constant at a given osmolyte concentration (Ks) relative to the binding...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
Ngày tải lên :
16/03/2014, 11:20
... iron box of desA (nucleotides 7–12 of the iron box), explaining the regulation of desA expression by binding of DmdR1 to the iron box The same overlapping was found in S pilosus DmdR1 binds to the ... Therefore, binding of the iron regulator DmdR1 will interfere with RNA polymerase interaction and expression of the desferrioxamine cluster Indeed, binding of the pure DmdR1 protein to the S coelicolor ... mediated by binding of the regulatory protein DmdR1 to the iron box located in the promoter region of the desABCD cluster Electrophoretic mobility-shift assays of the desA promoters of both S coelicolor...
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Báo cáo khoa học: Binding of hemolin to bacterial lipopolysaccharide and lipoteichoic acid An immunoglobulin superfamily member from insects as a pattern-recognition receptor doc
Ngày tải lên :
31/03/2014, 09:20
... Specific binding (demonstrated by competition with unlabeled hemolin) accounted for 70% of the hemolin binding to M lysodeikticus, 46% of the binding to E coli, and 25% of the binding to S cerevisiae ... test binding of different moieties of LPS to M sexta hemolin Binding of hemolin to immobilized LPS from a smooth strain of E coli (026:B6) was measured in the presence of a 20-fold excess of different ... Thus, binding of hemolin to Gram-positive and Gram-negative bacteria as well as yeast appears to involve specific binding sites, with a lower degree of specific binding to yeast cells than to bacteria...
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Báo cáo y học: "Differential binding of chemokines to macrophages and neutrophils in the human inflamed synovium" pps
Ngày tải lên :
09/08/2014, 03:24
... receptor autoradiography showing chemokine binding to the inflamed nonrheumatoid synovium (a) Samples of tissue were incubated with 125I-CCL5 and subjected to autoradiography The chemokine binds to ... biological effects of chemokines are mediated by binding to G-protein-coupled, seven transmembrane domain, chemokine receptors [11] CC receptors (CCR) bind members of the class of chemokines with ... venule in the subintima of inflamed, nonrheumatoid synovium (b) CXCL8 (IL-8) binding to macrophages in a macrophage-rich infiltrate in rheumatoid tissue (c) CXCL8 binding to macrophages in a lymphocyte-rich...
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Tolerance Level of Dissolved Oxygen to Feed into Anaerobic Ammonium Oxidation (anammox) Reactor
Ngày tải lên :
05/09/2013, 10:17
... concentrations of the two reactors The maximum nitrogen conversion rate of each reactor at the influent DO of about 0.2 mg/L was set to an anammox activity value of 1.0, and for the other concentrations of ... necessary primarily to mix the influent and to remove the nitrogen gas bubbles that formed on the surfaces of the gel carriers HRT was set to hours The to in the reactor was monitored and adjusted ... conversion rates over time of two reactors In reactor 1, the nitrogen conversion rate had decreased from 2.9 to 1.3 kg-N/m3/d when influent DO of mg/L had been fed into reactor After that, the DO...
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Tài liệu Báo cáo khoa học: Inorganic phosphate regulates the binding of cofilin to actin filaments pdf
Ngày tải lên :
19/02/2014, 07:20
... pertinent to the regulation of actin dynamics in the cell Results Fluorescence measurements of the effect of Pi on the rate of cofilin binding to TRC–F-actin A convenient way to study the binding of ... inhibitory effect of Pi on the subtilisin digestion indicates stabilization of the D-loop 300000 Fluorescence (A.U.) µM cofilin 200000 no cofilin 100000 Monitoring the binding of cofilin to F-actin ... prevents, the binding of actophorin to actin filaments In addition, actophorin was reported to promote the dissociation of Pi from freshly polymerized F-actin [34] However, no attempt was made to study...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
Ngày tải lên :
22/02/2014, 04:20
... cytosol, which stimulated the binding to chromatin Suppression of the binding of LBR to chromatin by phosphorylation with a kinase(s) in a mitotic phase egg cytosol Fig Stimulation of the binding ... protein kinase inhibitor (PKI), a PKA-specific inhibitor, could not suppress the stimulation of the binding of NK to chromatin Ó FEBS 2002 Regulation of the binding of LBR to chromatin (Eur J Biochem ... Identification of chromatin binding regions of LBR To analyze the binding of LBR to chromatin, we used the N-terminal portion of LBR, because this portion is responsible for the binding to chromatin...
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Tài liệu Báo cáo Y học: Binding of gelsolin domain 2 to actin An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin pptx
Ngày tải lên :
22/02/2014, 07:20
... 355–375 1.3 mM ND No binding No binding No binding No binding 2.9 mM mM 40 mM 30 mM mM 2.0 mM 2.0 mM ND ND No binding 50 mM 2.0 mM Binding ND No binding mM 1.5 mM ? No binding mM –2 mM mM 12 ... [33] are able to bind 355–375 of actin simultaneously We measured tight binding (Kd 1.8 mM ) to 114–375 and weaker binding (Kd 10 mM ) to 226–375 of actin As the affinity for S2 to the entire ... & Weeds, A.G (1994) Characterization of the F-actin binding domains of villin: classification of F-actin binding proteins into two groups according to their binding sites on actin FEBS Lett 338,...
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Báo cáo khoa học: Phosphorylation-dependent binding of human transcription factor MOK2 to lamin A⁄C pot
Ngày tải lên :
07/03/2014, 01:20
... results showed that none of the tested mutations was able to disrupt binding to hsMOK2 in vitro or in vivo However, we observed an aberrant cellular localization of hsMOK2 into nuclear aggregates ... software (Bio-Rad) Results were expressed as a percentage of binding to JSAP1 (21–101) domain (mean ± SD of three different experiments) with the GST–JLP 1–141 protein Furthermore, deletion of ... Ser46 Analysis of phosphomimetic mutations on hsMOK2 capacity to bind DNA Binding of hsMOK2 to DNA may be affected, either positively or negatively, by phosphorylation Therefore, to determine whether...
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Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
Ngày tải lên :
07/03/2014, 09:20
... the binding of the third metal ion suggesting, like Pullen et al [9], that rearrangement of the flap domain in HsSTP might lead to binding of a third metal ion The implications of the M3 binding ... calculated structure factor amplitudes, respectively Rfree is an R-factor for an unrefined subset of the data (5% of the data) a the metal ions are close to the B-factors of the coordinating ˚ residues ... Patterson figure of ˚ merit was 19 (resolution cut-off 3.0 A) Subsequent phasing with shelxe resulted in a correlation coefficient of 54% and allowed us to build a model of the protein into the Table...
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Báo cáo khoa học: Binding of the volatile general anesthetics halothane and isoflurane to a mammalian b-barrel protein doc
Ngày tải lên :
07/03/2014, 16:20
... anesthetic halothane to the hydrophobic core of porcine odorant binding protein The binding of halothane to the porcine odorant binding protein hydrophobic core was followed by tryptophan fluorescence ... (DG°) exceeded the heat of binding (DH°) by more than a factor of two (Table 1), indicating that binding to porcine odorant binding protein is entropy driven, in contrast to the results obtained ... (http://www.microcal.com/) This software provides the best-fit values for the heat of binding (DH°), the stoichiometry of binding (n), and the association constant (Ka) from plots of the heat evolved per mol of anesthetic...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
Ngày tải lên :
08/03/2014, 10:20
... with a Ki of 0.03 mM almost identical to that of native GalNAc-T1 On the other hand, C212S was more resistant to the treatment The Ki of 0.65 mM, may be due to a lower reactivity of Cys214 ... site of interaction with UDP-GalNAc, Cys214 is also involved in binding PCMPS and DFP modification of the serine-mutants of GalNAc-T1 We also examined the sensitivity of the three serine mutants to ... evaluation of the function of the three serine mutants, the kinetic properties of the mutant enzymes were compared to those of the parent enzyme, P-DN42 As shown in Table 1, the Km values of all...
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Báo cáo Y học: Characteristics of binding of insulin-like growth factor (IGF)-I and IGF-II analogues to the type 1 IGF receptor determined by BIAcore analysis pptx
Ngày tải lên :
08/03/2014, 22:20
... 2002 high-af®nity binding proteins (IGFBPs) with 10-fold higher af®nity than their binding to IGF receptors IGFBPs thereby in¯uence the availability of IGF to bind to IGF receptors [12] Over the ... between binding anities of IGF-I analogues to recombinant human IGF1R and prevention of apoptosis by IGF-I analogues The dissociation constants (Kd) for binding of IGF-I and IGF-I analogues to rhIGF1R ... the transmembrane domain of the high-af®nity IGF receptor We determined that the af®nity of the recombinant receptor for IGF was comparable to that of native type IGF receptors as measured using...
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Báo cáo Y học: Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC Fluorescence resonance energy transfer and crystallographic study ppt
Ngày tải lên :
08/03/2014, 23:20
... lying on the top of A146, which is stabilized by a hydrogen bond with the carbonyl oxygen of ˚ ˚ this residue (2.8 A) and the NE2 atom of H256 (2.8 A) The fact that C2-C9 carbon atoms of the lipid ... according to two binding modes (see Fig 6) These structural considerations suggest that the FRET was probably due to a molecular binding of TLL to cis-PnA Ó FEBS 2002 Lipase binding to lipid, ... amounts of cisPnA in the presence and absence of NaTDC The excitation wavelength was set to 280 nm and the emission wavelength ranged from 300 nm to 500 nm The accessibility of tryptophan to cis-PnA...
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Báo cáo khoa học: Nucleosome positioning in relation to nucleosome spacing and DNA sequence-specific binding of a protein doc
Ngày tải lên :
16/03/2014, 10:20
... but fails to tetramerize [29] Binding of R3 protein to a plasmid DNA pU6LNS (two lac operators at a distance of 183 bp) was reported A B Fig Relative positions of the three lac operator sites ... properties of binding of R3 to DNA are similar to those of the wild-type protein [34] Therefore, it appears that the observed protection is due to a positioned nucleosome encroaching on half of the ... boundary effect of the R3 pair bound to L1 and L2 Turning a gene into an active state from a state of inactivation often involves binding of a single activator molecule to its site in a repressive chromatin...
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Báo cáo khoa học: Binding of non-natural 3¢-nucleotides to ribonuclease A ppt
Ngày tải lên :
16/03/2014, 18:20
... purification Binding of non-natural 3¢-nucleotides to RNase A [16] The advantages of the route in Scheme include the mild conditions and high yield of the phosphorylation step, the use of commercially ... thiocarbonyldiimidazole to give Scheme Synthetic route to dUMP and dUFMP FEBS Journal 272 (2005) 744–755 ª 2005 FEBS 745 Binding of non-natural 3¢-nucleotides to RNase A C L Jenkins et al Scheme Synthetic route to ... The pKa of the phosphoryl group contributes to this order, as dianionic 3¢-nucleotides are known to be more potent inhibitors of RNase A than monoanionic 3¢-nucleotides [9] The pKa value of dUMP...
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Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx
Ngày tải lên :
17/03/2014, 17:20
... the interaction of annexin II with the cell surface receptors Binding of tPA to surfaces of matrilysin-treated cells It has been shown that tPA binds to annexin II on the surfaces of human endothelial ... enhancement of tPA binding to the cell surface was coincident with the release of cleaved annexin II The enhancement of tPA binding to the cell surface by matrilysin was confirmed when the amount of tPA ... activator, tPA binds to some membrane proteins, including annexin II [41] The binding of tPA or urokinase-type plasminogen activator to the membrane receptors greatly increases plasminogen activator...
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Báo cáo khoa học: Energetic coupling along an allosteric communication channel drives the binding of Jun-Fos heterodimeric transcription factor to DNA docx
Ngày tải lên :
22/03/2014, 16:20
... the binding of TRE duplex to various constructs of the Jun-Fos heterodimer were obtained from the fit of a one-site model, based on the binding of a ligand to a macromolecule using the law of mass ... the binding of TRE duplex to various constructs of the Jun-Fos heterodimer were obtained from the fit of a one-site model, based on the binding of a ligand to a macromolecule using the law of mass ... the binding of TRE duplex to various constructs of the Jun-Jun heterodimer were obtained from the fit of a one-site model, based on the binding of a ligand to a macromolecule using the law of mass...
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