binding of effector molecules
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... activepumping of the agents out of the cells and their pas-sive uptake [10,31] and (b) competition between theactive pumping of the agents out of the cells and theirintracellular binding to receptors ... (2001) Characterization of binding of leukotriene C4 by human multidrug resistance protein1. Evidence of differential interactions with NH2-andCOOH-proximal halves of the protein. J Biol Chem276, ... Subsequently, all of the upper phase, includingpart of the oil cushion, was removed, leaving a fraction of the oil above the cell pellets. The cell pellets were dissolvedby addition of 0.1 mL of guanidine...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
... amount of precipitation inthe presence of high concentrations of Arg-HClin the absence of the chaperone precluded analysis of the effect of this concentration on the protectiveability of aB-crystallin.Reduction-induced ... most likelyemploys various methods of binding (or binding modes) in order to prevent the aggregation of stressedproteins. Some of these binding modes (or binding sites) are favoured by phosphorylation ... the amount of precipita-tion of catalase by 190 ± 5% (Fig. 3A). The presence of aB-crystallin at a 1.0 : 0.5 w ⁄ w ratio of cata-lase : aB-crystallin inhibited the precipitation of cata-lase...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... DiscussionAction of reversible effectors on AAA activity of BuChEThe investigation of the effects of the ligands(tyramine, serotonin and benzalkonium) on the AAAand esterase activities of BuChE was ... wild-type enzymereflects the binding of benzalkonium to PAS.Effects of benzalkonium on ASCh hydrolysisUnder our experimental conditions, a study of theinhibition of ASCh hydrolysis was not possiblebecause ... higherconformational plasticity of the active site gorge of D70G compared with that of the wild-type enzyme foracylation with neutral ester.Effects of benzalkonium on ATMA hydrolysisHydrolysis of ATMA by wild-type...
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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... indicated.(B) The glycoforms of the protein bands were analyzed by calcula-tion of the percentages of the di- (d), mono- (j) and nonglycosy-lated (m) isoform as arithmetic means of separate gel runs. ... (B) Signal intensities of the di- (d), mono- (j)and nonglycosylated isoform (m) of PrPCwere quantified and calcu-lated as percentages of the total signal. The glycoforms of the pro-tein bands ... thedi-glycosylated isoform of PrPCthan did antibodies tothe structured core region. However, the glycoformpatterns of mouse PrPCalways showed the highest sig-nal intensity of the di-glycosylated isoform,...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... targets).Effects of DNA cis-platination on sequence-specific DNA binding of p73 proteinsWe tested the influence of DNA modification withcisplatin on the binding of two p73 isoforms, p73d andp73b, ... inhibitsp53 binding to a synthetic p53 DNA -binding site. Here we demonstratethat the effects of global DNA modification with cisplatin on binding of the p53 or p73 proteins to various p53 DNA -binding ... probe(Fig. 6A). Addition of either of the unmodified frag-ments (70 ng per sample) resulted in a partial decrease(by 35–45%) of the R53band intensities due to binding of a portion of the p53 molecule...
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Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... phospholipid and membrane binding of theother members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, includingthose of the binding of ICto phospholipid membranes,are ... PtdIns(3,4,5)P3.Involvement of the CPY -binding sites of ICin itsmembrane binding As IC–CPY has no affinity for phospholipid membranes,to obtain additional information on the involvement of the CPY -binding sites of ... membranes.ResultsMembrane -binding properties of ICIn an attempt to detect and characterize the membrane binding of IC, a member of the PEBP family, we firstperformed a liposome -binding assay of this inhibitorfor...
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Tài liệu Báo cáo khoa học: Inorganic phosphate regulates the binding of cofilin to actin filaments pdf
... regulation of actin dynamics in the cell.ResultsFluorescence measurements of the effect of Pion the rate of cofilin binding to TRC–F-actinA convenient way to study the binding of cofilin toF-actin ... fluorescence intensity of TRC–F-actin isdecreased > 70% upon binding of cofilin, while thefluorescence intensity of G-actin changes little uponaddition of cofilin [21]. The binding of 5 lm cofilin ... our observations, the rate of cofilin binding to F-actin in the presence of 2–30 mm Pi is the same at pH 6.5 and 8.0. The influ-ence of Pi on the relative rates of cofilin binding atthese two pH...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... complex [20]. Hence, themechanism of binding of fosfomycin by reinforcement of aninitial binding site (mainly K22 and R397) through recruit-ment of secondary binding partners located in a flexibleloop ... involved in fosfomycin binding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not leadto a complete loss of fosfomycin binding. Therefore ... evidence from studies of small angleX-ray scattering [14] and the protective effect of UDPNAGon proteolysis of MurA [15]. Here we report the thermo-dynamic profile of UDPNAG binding to two MurA...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... retarda-tion. Iron loading of HepG2 cells resulted in increased expression of Nor3.2-reactive CD1d molecules at the plasma membrane. Expression of classical MHC class I and II molecules, ICAM-1 and ... appearance of Nor3.2-reactive CD1d molecules at the cell surface of iron-loaded HepG2 cells could be a consequence of CD1d misfolding in intracellular compartments with asubsequent release of the ... of CD1d molecules at the cellsurface of iron-loaded HepG2 cells in a non-nativeform may have implications at two different levels; atthe level of the hepatic cell itself and at the level of...
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Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
... infavour of a specific binding of phosphate to cyto-chrome c3[23] instead of a simple electrostatic effect of increased ionic strength at least up to 0.2 m concen-tration. The region of positively ... approximation of the redox centres of the donor and acceptor, and thatthe reduction potentials ensure a favourable drivingforce, which is one of the main determinants of therate of electron ... Experimental measure-ments of the reduction potentials of proteins involvedin complexes have been reported [5–7], but the effect of partner binding on the microscopic properties of theredox centres...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
... theamplification of the 3¢ end of the b-gal gene. The presence of similar amounts of genomic DNA in the reactions wascontrolled by concomitant addition of primers that amplifya 200 bp region of the RNA ... isolation by FACS of living cells expressing lowlevels of antigen.DiscussionThe potential of scFvs for demonstrating the presence of targeted protein antigens in the cytoplasm of mam-malian ... intracellularforms of antigen.To analyze how these scFvs linked to EGFP ord1-EGFP behave in case of low and constant expres-sion of b-gal, we took advantage of the availability of two cell lines,...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... using deletion mutants of the 3¢ terminus of the minus-strand RNA but deletionswere made on the basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minus-strand ... 3’domains of plus or minus-strand HCV RNA.(B) RNA products synthesized by HCV NS5Bin the presence of heparin with wild-type or5’ deleted (–)IRES RNA. Binding and replication of 3¢-end of HCV ... changingthe Not1-Age 1 fragment of the pCV-UTR4 with thecorresponding fragment deleted from all nucleotides of SLB1T. Astier-Gin et al. Binding and replication of 3¢-end of HCV minus RNAFEBS Journal...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
... Therefore, HP1may not participate in the binding of LBR to spermchromatin in eggs. In t he case of the binding of LBR toFig. 7 . Suppression of the b inding of LBR fragments to chromatin byphosphorylation ... of thearginine-serine repeat-region in the N-terminal portion of LBR by an LBR kinase inhibits the binding of p34 proteinCorrespondence to T. Horigome, Department of Biochemistry,Faculty of ... of binding of LBR tochromatin, beads bearing LBR fragments were preincu-bated with a DNA solution and then the binding tochromatin was examined (Fig. 3, h atched c olumn s). T he binding of...
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Tài liệu Báo cáo Y học: Binding of gelsolin domain 2 to actin An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin pptx
... ?–1–10 No binding ND No binding [51]18–28 No binding ND 3 mMa84–103 No binding No binding 1–2 mMa112–125 No binding 50 mM 4 mM [51]119–132 2.9 mM 2.0 mM 12 mM [51]347–365 2 mM Binding ... Weeds, A.G. (1994)Characterization of the F-actin binding domains of villin:classification of F-actin binding proteins into two groups accordingto their binding sites on actin. FEBS Lett. 338, ... F-actin binding of domain 2 with respect to the F-actin severing and cappingactivity of the whole gelsolin molecule.Keywords: actin; actin -binding proteins; cofilin; gelsolin.The organization of...
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Tài liệu Báo cáo Y học: EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates potx
... bound enzymeforms. Our study offers the first EPR description of important catalytic states of the dioxygenation process of flavonols and shows that the anaerobic binding of flavonolsproduces clear ... alter the EPR spectra of the enzymeindicating the absence of binding to the copper centre. Fromthe chemical structures of the tested flavonoids we concludethat the presence of a free 3-OH group ... specificity of 2,3QD for flavonol binding has beentested by anaerobically incubating the enzyme with differentflavonoids. The addition of a flavone (apigenin), of aflavanonol (taxifolin) and of a flavan-3-ol...
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- have been studied as potential key molecules in the pathogenesis of the precancerous intestinal metaplasia of the human stomach however
- in this series of four minireviews the field of scaffold proteins and proteins of similar molecular⁄cellular functions is overviewed by binding and bringing into proximity two or more signaling proteins
- concept of hybrid molecules
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