binding of effector molecules

... active pumping of the agents out of the cells and their pas- sive uptake [10,31] and (b) competition between the active pumping of the agents out of the cells and their intracellular binding to receptors ... (2001) Characterization of binding of leukotriene C4 by human multidrug resistance protein 1. Evidence of differential interactions with NH2-and COOH-proximal halves of the protein. J Biol Chem 276, ... Subsequently, all of the upper phase, including part of the oil cushion, was removed, leaving a fraction of the oil above the cell pellets. The cell pellets were dissolved by addition of 0.1 mL of guanidine...

Ngày tải lên: 18/02/2014, 13:20

... amount of precipitation in the presence of high concentrations of Arg-HCl in the absence of the chaperone precluded analysis of the effect of this concentration on the protective ability of aB-crystallin. Reduction-induced ... most likely employs various methods of binding (or binding modes) in order to prevent the aggregation of stressed proteins. Some of these binding modes (or binding sites) are favoured by phosphorylation ... the amount of precipita- tion of catalase by 190 ± 5% (Fig. 3A). The presence of aB-crystallin at a 1.0 : 0.5 w ⁄ w ratio of cata- lase : aB-crystallin inhibited the precipitation of cata- lase...

Ngày tải lên: 18/02/2014, 16:20

... Discussion Action of reversible effectors on AAA activity of BuChE The investigation of the effects of the ligands (tyramine, serotonin and benzalkonium) on the AAA and esterase activities of BuChE was ... wild-type enzyme reflects the binding of benzalkonium to PAS. Effects of benzalkonium on ASCh hydrolysis Under our experimental conditions, a study of the inhibition of ASCh hydrolysis was not possible because ... higher conformational plasticity of the active site gorge of D70G compared with that of the wild-type enzyme for acylation with neutral ester. Effects of benzalkonium on ATMA hydrolysis Hydrolysis of ATMA by wild-type...

Ngày tải lên: 18/02/2014, 17:20

... indicated. (B) The glycoforms of the protein bands were analyzed by calcula- tion of the percentages of the di- (d), mono- (j) and nonglycosy- lated (m) isoform as arithmetic means of separate gel runs. ... (B) Signal intensities of the di- (d), mono- (j) and nonglycosylated isoform (m) of PrP C were quantified and calcu- lated as percentages of the total signal. The glycoforms of the pro- tein bands ... the di-glycosylated isoform of PrP C than did antibodies to the structured core region. However, the glycoform patterns of mouse PrP C always showed the highest sig- nal intensity of the di-glycosylated isoform,...

Ngày tải lên: 19/02/2014, 02:20

... targets). Effects of DNA cis-platination on sequence- specific DNA binding of p73 proteins We tested the influence of DNA modification with cisplatin on the binding of two p73 isoforms, p73d and p73b, ... inhibits p53 binding to a synthetic p53 DNA -binding site. Here we demonstrate that the effects of global DNA modification with cisplatin on binding of the p53 or p73 proteins to various p53 DNA -binding ... probe (Fig. 6A). Addition of either of the unmodified frag- ments (70 ng per sample) resulted in a partial decrease (by 35–45%) of the R 53 band intensities due to binding of a portion of the p53 molecule...

Ngày tải lên: 19/02/2014, 05:20

... phospholipid and membrane binding of the other members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, including those of the binding of I C to phospholipid membranes, are ... PtdIns(3,4,5)P 3 . Involvement of the CPY -binding sites of I C in its membrane binding As I C –CPY has no affinity for phospholipid membranes, to obtain additional information on the involvement of the CPY -binding sites of ... membranes. Results Membrane -binding properties of I C In an attempt to detect and characterize the membrane binding of I C , a member of the PEBP family, we first performed a liposome -binding assay of this inhibitor for...

Ngày tải lên: 19/02/2014, 05:20

... regulation of actin dynamics in the cell. Results Fluorescence measurements of the effect of Pi on the rate of cofilin binding to TRC–F-actin A convenient way to study the binding of cofilin to F-actin ... fluorescence intensity of TRC–F-actin is decreased > 70% upon binding of cofilin, while the fluorescence intensity of G-actin changes little upon addition of cofilin [21]. The binding of 5 lm cofilin ... our observations, the rate of cofilin binding to F-actin in the presence of 2–30 mm Pi is the same at pH 6.5 and 8.0. The influ- ence of Pi on the relative rates of cofilin binding at these two pH...

Ngày tải lên: 19/02/2014, 07:20

... complex [20]. Hence, the mechanism of binding of fosfomycin by reinforcement of an initial binding site (mainly K22 and R397) through recruit- ment of secondary binding partners located in a flexible loop ... involved in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a single interaction would not lead to a complete loss of fosfomycin binding. Therefore ... evidence from studies of small angle X-ray scattering [14] and the protective effect of UDPNAG on proteolysis of MurA [15]. Here we report the thermo- dynamic profile of UDPNAG binding to two MurA...

Ngày tải lên: 19/02/2014, 13:20

... retarda- tion. Iron loading of HepG2 cells resulted in increased expression of Nor3.2-reactive CD1d molecules at the plasma membrane. Expression of classical MHC class I and II molecules, ICAM-1 and ... appearance of Nor3.2-reactive CD1d molecules at the cell surface of iron-loaded HepG2 cells could be a consequence of CD1d misfolding in intracellular compartments with a subsequent release of the ... of CD1d molecules at the cell surface of iron-loaded HepG2 cells in a non-native form may have implications at two different levels; at the level of the hepatic cell itself and at the level of...

Ngày tải lên: 19/02/2014, 16:20

... in favour of a specific binding of phosphate to cyto- chrome c 3 [23] instead of a simple electrostatic effect of increased ionic strength at least up to 0.2 m concen- tration. The region of positively ... approximation of the redox centres of the donor and acceptor, and that the reduction potentials ensure a favourable driving force, which is one of the main determinants of the rate of electron ... Experimental measure- ments of the reduction potentials of proteins involved in complexes have been reported [5–7], but the effect of partner binding on the microscopic properties of the redox centres...

Ngày tải lên: 19/02/2014, 17:20

... the amplification of the 3¢ end of the b-gal gene. The presence of similar amounts of genomic DNA in the reactions was controlled by concomitant addition of primers that amplify a 200 bp region of the RNA ... isolation by FACS of living cells expressing low levels of antigen. Discussion The potential of scFvs for demonstrating the presence of targeted protein antigens in the cytoplasm of mam- malian ... intracellular forms of antigen. To analyze how these scFvs linked to EGFP or d1-EGFP behave in case of low and constant expres- sion of b-gal, we took advantage of the availability of two cell lines,...

Ngày tải lên: 19/02/2014, 18:20

... using deletion mutants of the 3¢ terminus of the minus-strand RNA but deletions were made on the basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minus- strand ... 3’ domains of plus or minus-strand HCV RNA. (B) RNA products synthesized by HCV NS5B in the presence of heparin with wild-type or 5’ deleted (–)IRES RNA. Binding and replication of 3¢-end of HCV ... changing the Not1-Age 1 fragment of the pCV-UTR4 with the corresponding fragment deleted from all nucleotides of SLB1 T. Astier-Gin et al. Binding and replication of 3¢-end of HCV minus RNA FEBS Journal...

Ngày tải lên: 20/02/2014, 01:20

... Therefore, HP1 may not participate in the binding of LBR to sperm chromatin in eggs. In t he case of the binding of LBR to Fig. 7 . Suppression of the b inding of LBR fragments to chromatin by phosphorylation ... of the arginine-serine repeat-region in the N-terminal portion of LBR by an LBR kinase inhibits the binding of p34 protein Correspondence to T. Horigome, Department of Biochemistry, Faculty of ... of binding of LBR to chromatin, beads bearing LBR fragments were preincu- bated with a DNA solution and then the binding to chromatin was examined (Fig. 3, h atched c olumn s). T he binding of...

Ngày tải lên: 22/02/2014, 04:20

... ?– 1–10 No binding ND No binding [51] 18–28 No binding ND 3 m M a 84–103 No binding No binding 1–2 mM a 112–125 No binding 50 mM 4 mM [51] 119–132 2.9 m M 2.0 mM 12 mM [51] 347–365 2 m M Binding ... Weeds, A.G. (1994) Characterization of the F-actin binding domains of villin: classification of F-actin binding proteins into two groups according to their binding sites on actin. FEBS Lett. 338, ... F-actin binding of domain 2 with respect to the F-actin severing and capping activity of the whole gelsolin molecule. Keywords: actin; actin -binding proteins; cofilin; gelsolin. The organization of...

Ngày tải lên: 22/02/2014, 07:20

... bound enzyme forms. Our study offers the first EPR description of important catalytic states of the dioxygenation process of flavonols and shows that the anaerobic binding of flavonols produces clear ... alter the EPR spectra of the enzyme indicating the absence of binding to the copper centre. From the chemical structures of the tested flavonoids we conclude that the presence of a free 3-OH group ... specificity of 2,3QD for flavonol binding has been tested by anaerobically incubating the enzyme with different flavonoids. The addition of a flavone (apigenin), of a flavanonol (taxifolin) and of a flavan-3-ol...

Ngày tải lên: 22/02/2014, 07:20

Ngày tải lên: 06/03/2014, 00:20

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Ngày tải lên: 07/03/2014, 16:20