basic approaches to gene expression analysis of stem cells by microarrays

... averaged profile Expression profiles of both control and experimental groups are then scaled to the averaged profile of the control group The two main sources of heterogeneity in gene expression ... independent of the level of gene expression The two main sources of heterogeneity in gene expression variations — additive and multiplicative components — are excluded in our analysis by eliminating expression ... Differential gene expression analysis of active disease Statistical analysis of the difference of gene expression in samples from patients and 12 healthy controls gave the following results 1716 genes of...

Ngày tải lên: 09/08/2014, 01:23

... Comparison of apple and tomato expression Comparison of apple and tomato expression Expression of tomato and apple genes identified as changing during fruit development and similar by sequence ... apple and tomato microarrays was plotted for several of the genes identified The top five genes in each cluster by quality of the BLAST match between apple and tomato were plotted Several genes possibly ... one tomato sequence had homology to an apple gene and in the case of the tubulin genes to three apple genes For the tubulin genes the patterns of expression mostly differed between apple and tomato...

Ngày tải lên: 12/08/2014, 05:20

... germination of the seed (Figures 1L, 1M) A crosssection of the cotyledon shows differentiation of the cortical cells into a layer of palisade cells and the compact mesophyll cells The mesophyll cells of ... flax unigenes encoding transcription factors that are known embryogenesis regulators Tissue distribution of flax unigenes encoding ESTs with similarity to important regulators of embryogenesis ... adequate for in silico expression analysis Assembly of the ESTs resulted in 30,640 unigenes and 82% of these could be identified on the basis of homology to known and hypothetical genes from other...

Ngày tải lên: 11/08/2014, 11:22

... oligonucleotide microarrays to gain gene expression profiles of Nfia-/- mouse brains and of corresponding wild-type samples Relatively early and late changes in gene expression were measured by quantifying ... differentiation According to this model, nuclear factor IA (NFI-A) suppresses expression of genes related to oligodendrocyte precursor cells and activates expression of genes whose products are ... high levels of expression, leading to an overexpression at P16 By contrast, genes whose expression usually increases during the course of terminal differentiation after birth appear not to be activated...

Ngày tải lên: 14/08/2014, 07:21

... factor 1-α (Hif-1α), a heterodimeric transcription factor to induce expression of genes that in turn trigger hematopoiesis and development of structures forming the circulatory system, thereby ... 93 Gene expression profile of cranial neural tubes in embryos of control vii TABLE OF CONTENTS and diabetic mice…………………………………………………………………… 94 Effect of maternal diabetes on expression of genes ... GLOBAL GENE EXPRESSION ANALYSIS OF CRANIAL NEURAL TUBES IN EMBRYOS OF DIABETIC MICE JIANG BORAN MBBS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ANATOMY YONG LOO...

Ngày tải lên: 11/09/2015, 16:07

... arose by duplication of a common ancestral gene encoding these specific domains In vivo expression and response pattern of gcGLUT to short and long-term hypoxia Fig Phylogenetic analysis of gcGLUT ... phylogenetically more similar to GLUT3 than to other class I GLUTs Comparative analysis of the genomic organization of gcGLUT with different human GLUT genes showed that exons 4–9 of the gcGLUT gene, ... GLUT3 expression in specific tissues to enhance the uptake rate of glucose both to facilitate the supply of metabolic energy [9] and to protect cells from hypoxic injury [26] Fish often have to contend...

Ngày tải lên: 17/03/2014, 03:20

... Genomic structure and expression analysis of the Cc RNase Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of a cDNA sequence of 864 bp (Cc RNase ... Laboratories, Palo Alto, CA, USA) as described by the manufacturer In order to isolate the alternative Cc RNase Genomic structure and expression analysis of the Cc RNase cDNA, a modified version of ... structure and expression analysis of the Cc RNase Expression and purification of Cc RNase recombinant protein The ORF of the Cc RNase cDNA 02 sequence was amplified by PCR and then sub-cloned into the...

Ngày tải lên: 23/03/2014, 06:20

... for gene expression It is known that introns may participate in gene regulation, by modulating the level of expression and ⁄ or determining the specific pattern of expression of a gene [34–39] To ... regulation of cardosin B gene expression Comparison of the leader intron of the cardosin B gene with pistil-specific genes revealed the presence of putative regulatory elements A region of SLG13, a gene ... reported to bind to the basic pentacysteine protein, and may be involved in the regulation of the expression of a diversity of genes [51] Moreover, several inverted repeats are known to regulate gene...

Ngày tải lên: 30/03/2014, 09:20

... Two out of of these matched controls Table Enriched gene ontology terms for genes upregulated in bleomycin sulphate treated fibroblasts compared to controls Gene ontology term a Number of genes ... median centred Genes for which expression values were present in less than 80% of samples were excluded Significance Analysis of Microarrays (SAM) [17] was performed to identify genes differentially ... http://www.ro-journal.com/content/6/1/42 Page of Table Enriched gene ontology terms for genes down-regulated in bleomycin sulphate treated fibroblasts compared to controls Gene ontology term a Cell cycle 82 6.00E-16 Mitotic cell...

Ngày tải lên: 09/08/2014, 09:20

... Concordance of gene expression determined by microarrays and RT-PCR Expression ratios of representative genes in (a) cerebellum and (b) white adipose tissue identified as outliers by microarrays ... biological system as evidenced by the coordinate expression of functionally related genes To gain insight into the pathways most perturbed in our models, we analyzed the expression patterns of genes ... immune -gene expression, and in the induction of antiproliferative genes Lastly, our analysis further confirms that the relative levels of TR isoforms lead to dramatic differential effects on gene expression...

Ngày tải lên: 09/08/2014, 20:20

... 7) The use of regulatory elements of these highly expressed genes may be an excellent tool for conferring strong expression to a target gene in transgenesis approaches To identify genes uniquely ... shown to function as negative regulators of gene expression mediated by auxin response factor (ARF) A gene similar to auxin receptor TIR1 that promotes ubiquitin (Ub)-mediated degradation of Aux/IAA ... increase of photosystem I and II genes (CAB and PSI-E subunit) may be a mechanism to sustain photosystems susceptible to ROS attack [80] These results indicate that the activation of the photosynthetic...

Ngày tải lên: 11/08/2014, 11:21

... as transcription factors Expression analysis of BnWRKY genes in response to fungal pathogens-S sclerotiorum and A brassicae Because the divergence of paralogous genes is often after the sub-functionalization ... resistance in Arabidopsis and tobacco through modulation of SA- or JA-responsive gene expression similar to that induced by the TGA class of basic leucine-zipper transcription factors [39,40,45,81,112] ... examined the responses of a few additional canola genes that are proposed to be orthologs of Arabidopsis genes previously reported to respond to these hormones These Arabidopsis genes were two bZIP...

Ngày tải lên: 12/08/2014, 03:20

... regulated by Hfe disruption by mouse strain and organ studied Genes regulated by Hfe disruption identified by statistical analysis of microarrays (SAM) were filtered to summarize the number of upregulated ... measures of mRNA expression in livers of 35 adult BXD recombinant inbred male mice obtained with Agilent G4121A microarrays, was used to link expression of the genes in this list to genetic markers ... number of genes involved in the term The expression analysis systematic explorer (EASE) score is a modified Fisher exact P value [51] BP, biological process; GO, Gene Ontology Correlation of expression...

Ngày tải lên: 14/08/2014, 08:20

... Validation of selected genes by flow cytometry and immunocytochemistry Validation of selected genes by flow cytometry and immunocytochemistry (a) Flow cytometry analysis of prostate cancer cells co-stained ... cancer stem cell phenotype By comparing RNA expression patterns from stem and committed cells, independent of their disease status, 287 probesets showed significantly elevated expression in stem cells ... studies of prostate have been dominated by androgen receptor-regulated gene products derived from more abundant differentiated cells and the higher average gene expression in these cells is likely to...

Ngày tải lên: 14/08/2014, 08:21

... some stem cells can survive irradiation probably due to activation of some genes that are involved in stem cell protective mechanisms To select these genes, it is necessary to eliminate the genes ... Figure PCR analysis of expression of 10 selected genes differentially regulated in 30 Gy irradiated planarians and controls Real-time Real-time PCR analysis of expression of 10 selected genes differentially ... it is not possible to distinguish between contributions made by single cell types to the genetic profile Further studies of the expression of single factors are necessary to ascertain their specific...

Ngày tải lên: 14/08/2014, 20:22

... Induction of stem cells to cardiomyocytes 8! Use of Xenopus laevis in research 9! Microarray analysis of gene expression 13! Gene Ontology 15! Scope of this ... differential gene expression analysis of Nkx2-5 overexpression led to the discovery of 99 potential targets of Nkx2-5 Howard J Edenberg, Ph.D., Chair vii TABLE OF CONTENTS List of Tables ... Semi-quantitative RT-PCR profile of selected genes 89! Figure 3.9 – Standard curves for qPCR profiled genes 92! Figure 3.10 – qPCR expression profiles of selected genes (normalized to ODC) 98!...

Ngày tải lên: 24/08/2014, 12:29

... the analysis 2.2 Clustering Expression Profiles Microarrays provide a deluge of gene expression data by simultaneous measurement of expression levels of thousands of genes This large amount of gene ... Loading vectors of PCA Pi p-value of gene i S Covariance matrix of gene expression data t number of time-points X Gene expression data xx zi Scores vectors zi∆ Difference of scores for ith gene Z∆ Difference ... operation of bioprocesses The advent of microarray technology has created a deluge of gene expression data by virtue of its ability to measure the expression levels of thousands of genes simultaneously...

Ngày tải lên: 11/09/2015, 09:01

... Immunofluorescence localization of TTMP in HeLa cells 76 17 Effect of forced expression of TTMP on cell proliferation in CD18 pancreatic cancer cells 77 Effect of forced expression of ... region of the hTTMP gene 85 24 Deletion analysis of the 5’ flanking region of the hTTMP gene 86 25 Mutational analysis of the proximal promoter region of the hTTMP gene 87 26 Electrophoretic ... exocrine cells (1-5) Members of the EGF family of growth factors such as EGF, TGFα and betacellulin can bind to EGF receptors expressed on pancreatic islet cells, acinar cells, and ductal cells...

Ngày tải lên: 14/09/2015, 22:09

... development of 28 arterial plaque Figure 12 A total of models will be used, we can add ml of cells ~ x 105 cells to ml of RPMI 1640 with cycloheximide added for all flasks 48 Figure 13 An illustration of ... List of selected cellular genes, with respective gene 82 specific primers designed Table Tabulated results of real time CT values of the 29 85 selected genes subjected to the T test vi List of ... cell division cycle 2, G1 to S and G2 to M (CDC2) gene In relation to the atherosclerosis, the results of the selected bands generally seem to exhibit two main modes of action One is involved...

Ngày tải lên: 03/10/2015, 11:36

... 51 51 RESULTS Expression analysis of zfgfap-l Temporal expression analysis of zfgfap-l Expression analysis of zfgfap-l by whole-mount in situ hybridization Expression analysis of zfgfap-l in ... commitment of ectodermal cells to a neural progenitor fate (Campos-Ortega., 1993; Modolell., 1997) Proneural clusters are characterized by expression of proneural genes (belonging to the bHLH family) Cells ... and leads to the formation of two layers of cells- epiblast and hypoblast The cells of the epiblast and hypoblast converge to the dorsal side of the embryo to create the primordium of the body...

Ngày tải lên: 05/10/2015, 22:31