... dose B pseudomallei infected C5 7BL/6 mice died of septicemia resembling the characteristics of low dose infected BALB/ cmice C5 7BL/6 mice which survived after an initial infection were found to be ... primer: 5’-ACT GCC ACG GCA CAG TC-3’; reverse primer: 5’-CCG CTT CCT GAG GCT G-3’), and Glyseraldehyde-3-phosphate dehydrogenase (GAPDH, forward primer: 5’-TTC ACC ACC ATG GAG AAG GC-3’; reverse ... CFU/20 μl) of bacteria as indicated, control mice where inoculated with PBS At various time points after infection, mice were anesthetized and blood was collected by cardiac puncture from mice...
... tried to detect and characterize such an enzyme in various fungi As described below, we succeeded in characterizing the production and reaction mechanism of a novel secretory enzyme that cleaves b-aryl ... DHP-GOU (Ca alcohol type) For EC and CY, reactions were initiated by addition of 0.1 mL of EC or CY to 1.0 mL of VM containing 10 lg of GOU For HP and M, reactions were initiated by addition of 0.1 ... wood chips as the sole carbon source In addition, 2BW-1 also grew in the lignin-related compounds, p-hydroxybenzoic acid, gallic acid and vanillic acid, as a sole source of carbon Cell growth and...
... 5¢-ATAGTTTAGCGGCCG CTTACTTCCGGCGGATGATGAGCGAG-3¢ for e1–219, (b) 5¢-ATAGTTTAGCGGCCGCTTAGTGATGGTGATG GTGATGCTTCCGGCGGATGATGAGCGAG-3¢ for e1– 219HIS, (c) 5¢-ATAGTTTAGCGGCCGCTTACGGCTT CCGGCGGATGATGAGCGAG-3¢ for ... al AGCAGG-3¢ for c1 –218HIS (c1 –218 with a 6-HIS tag at its 3¢ end to facilitate purification), (c) 5¢-ATAG TTTAGCGGCCGCTTAGGGCTTGCGCTGGATGAGCA GG-3¢ for c1 –219, or (d) 5¢-ATAGTTTAGCGGCC GCTTAGTGATGGTGATGGTGATGGGGCTTGCGCT ... glycosylated, like native AChR [28] (Fig 2C) Even though we lack direct evidence about the site and structure of the glycosylation sites on the ECDs, indirect evidence of correct glycosylation of...
... Journal of Circadian Rhythms 2007, 5:7 http://www.jcircadianrhythms.com/content/5/1/7 Figure inbred mice Circadian period in hours of B6, C1 2A, and A/J strainsof Circadian period in hours of B6, C1 2A, ... and A/J strainsof inbred mice Error bars represent the SEM Daily profile of locomotorLD 12:1 2of representativecircadian Figure time the B6 and AhR strains in activity as a function ofmiceof ... Daily profile of locomotor activity of representative miceof the B6 and AhR strains in LD 12:12 as a function of circadian time Profiles were generated in the "Activity Profile" module in Clocklab...
... 32 raccoon F vacc C V+/W+ 33 raccoon F vacc C V-/W- 34 raccoon F vacc C V-/W+ 35 raccoon F vacc C V-/W+ 36 raccoon M vacc S V-/W- 37 raccoon M vacc S V-/W+ 38 raccoon M vacc C V-/W- 39 raccoon ... dog F vacc C V-/W- 10 dog F vacc C V+/W+ 11 dog M vacc C V+/W- 12 dog M vacc C V-/W- 13 dog F vacc C V+/W- 14 dog F 26 NI N V+/W- 15 dog F vacc S V+/W- 16 dog M vacc C V-/W- 17 dog M vacc C V-/W- ... record Clinical Form of distemper RT-nPCR dog M vacc C V-/W+ dog M vacc C V+/W+ dog M vacc C V-/W+ dog F vacc C V+/W- dog F 36 no N V-/W+ dog M vacc C V-/W- dog F vacc S V+/W- dog M vacc S V-/W+...
... ascorbic acid in 2.5 mM and ascorbic acid in 10 mM (Figure 6) Therefore, migration was inhibited according to ascorbic acid concentration in cancer cell and the treatment time Conclusion Ascorbic ... injecting ascorbic acid into mice injected with cancer cells led to an increased survival rate over mice injected with cancer cells only, both when ascorbic acid was provided preventively and ... dose concentration ascorbic acid given twice Group G: high dose concentration ascorbic acid twice for days, S-180 cells same as Group D, and then high dose concentration ascorbic acid given twice...
... statistically significant increase of IL-12 after the challenge Concentrations of the cytokine retained constant in infected mice, except the unexpected decline occurred on day The expression of IL-18 ... can conclude that basic model parameters were characterized and virus A/duck/Tuva/01/06 can be used to evaluate anti-influenza vaccines and therapeutics Figure 01/06 Gross pathology of BALB/ c ... terminator Cycle Sequencing according instructions of manufacturer Primers for sequence were obtained from E Hoffman (SJCRH, Memphis, TN) Sequence products were analyzed on automatic sequence analyzer...
... statistically significant increase of IL-12 after the challenge Concentrations of the cytokine retained constant in infected mice, except the unexpected decline occurred on day The expression of IL-18 ... can conclude that basic model parameters were characterized and virus A/duck/Tuva/01/06 can be used to evaluate anti-influenza vaccines and therapeutics Figure 01/06 Gross pathology of BALB/ c ... terminator Cycle Sequencing according instructions of manufacturer Primers for sequence were obtained from E Hoffman (SJCRH, Memphis, TN) Sequence products were analyzed on automatic sequence analyzer...
... Immunohistochemical Study of the Pancreatic Endocrine cells in the BALB/ c mice: An Unique Distributional Pattern of Glucagon 171 35 Fig Somatostatin- and PP-immunoreactive cells in the pancreas of the BALB/ c ... form and cyclic form This substance inhibited the secretion of the gastrin, cholecystokinin, 40 secretin, glucagon, insulin, motilin and gastric acid and the absorption of amino acid, glucose and ... which had been preincubated with its corresponding antigen and the relative frequency of occurrence of each type of immunoreactive cells was placed into one of five categories according to their...
... The cDNA was then used as a template for PCR reactions with primers specific for iNOS or GAPDH The sequences of the primers used to amplify iNOS were 5'-CCTCCTCC ACCCTACCAAGT-3' and 5'-CACCCAAAGTGCTTCA ... 5'-CACCCAAAGTGCTTCA GTCA-3' (Gene Bank Accession No NM010927), and the sequences of the primers used to amplify GADPH were 5'-AACGGATTTGGTCGTATTGG-3' and 5'-AGCCTTC TCCATGGTGGTGAAGAC-3' (Gene Bank Accession ... T, Nathan C Cloning and characterization of inducible nitric oxide synthase from mouse macrophages Science 1992, 256, 225-228 Xie QW, Kashiwabara Y, Nathan C Role of transcription factor NF-κB/Rel...
... GGC TCG CTC GGT GAC CCT AGT CTT T TCT GCA GGC GTA TGT ATC AGT CTC A GAA AGC CTA GAA AGT CTG AAT AAC T ATC AGC AGC GAC TCC TTT TCC GCT T AGC CCA CGT CGT AGC AAA CCA CCA A ACA CCC ATT CCC TTC ACA ... GAG CAA T TCA TGG GAT GAT GAT GAT AAC CTG CT CCC ATA CTT TAG GAA GAC ACG GAT T ATG TAC AGC ATG CAG CTC GCA TCC TGT GTC A AGT CAA ATC CAG AAC ATG CCG CAG AGG TCC A ACA AAA ATC ACT TGA GAG AGA TCA ... GAG AGA TCA T AGT AAT CCA TTT GCA TGA TGC TCT T ACC TGG TAG AAG TGA TGC CCC AGG CA CTA TGC AGT TGA CTA AGA TGT CAA A CCA AGA ACT TGC AGC TGA AG TGG GTC TAT TCC GTT GTG TC Size (bp) 300 388 446...
... naive and proteoglycan-immunized (not-yet-arthritic) miceof BALB/ cJ and BALB/ cByJ colonies (n = of each, four-group cross-comparison: naïve BALB/ cJ versus immunized BALB/ cJ, naïve BALB/ cByJ ... BALB/ cJ versus four-group cross-comparison: naïve BALB/ cJ versus immunized BALB/ (not-yet-arthritic) miceof BALB/ cJ and BALB/ cByJ expressed = of each, significant levels in spleen cells of naive ... hyaluronan and 'cartilage-specific' PG aggrecan, and the core protein of the human aggrecan molecule has over 100 predicted and at least 27 confirmed T-cell epitopes in BALB/ cmice [9,74] A number of...
... eosinophilic bronchitis without asthma Lancet 1989, 17(8651):1346-1348 Brightling CE: Chronic Cough Due to Nonasthmatic Eosinophilic Bronchitis ACCP Evidence-Based Clinical Practice Guidelines Chest ... determination of airway resistance and dynamic compliance, which are the gold standards in assessing bronchoconstriction Recently, unrestrained barometric plethysmography in conscious mice or rats ... in conscious mice might include the contribution of upper airway resistance and it could reflect airflow limitation in the upper airway, including the nasal cavity, larynx and pharynx, etc As...
... http://respiratory-research.com/content/8/1/6 Figure of Course HMPV and RSV infection in the BALB/ cmice Course of HMPV and RSV infection in the BALB/ cmice (A) Kinetics of HMPV replication in the respiratory tract ... infection Percentage of (A) CD8+, (B) CD4+, and (C) IFN-γ-producing CD8+ cells of total BAL lymphocytes as determined by FACS analysis of BAL cells from HMPV- or RSVinfected BALB/ cmice * P < 0.05, ... (D) Percentage of NK cells (DX5+/CD3-) of total BAL lymphocytes in the BAL of HMPV- or RSV-infected BALB/ Cmice (2 × 105 PFU/mouse), and (E) NK cell-mediated cytotoxicity in mice infected with...
... Escherichia coli O157:H7-infected BALB/ cmice Annals of Clinical Microbiology and Antimicrobials 2011 10:34 Page of Submit your next manuscript to BioMed Central and take full advantage of: • Convenient ... treatment of infected mice, seven groups each containing mice were used (Table 1) Mice received × LD50 of the CDC 26-98 strain of E coli O157:H7 and then were treated with the in vivo MIC equivalent ... eradicate the bacterial agent Page of The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of rifampicin and gentamicin for the CDC 26-98 strain E coli O157: H7...