... importantly, these results indicate that measurement of heme content is useful for evaluation of heme dynamics in cultured cells Role of heme metabolism in cellular heme content Regulatory role of free ... HO-1, siHO-1, which was reported by Miralem et al [53], was used HO-2 siRNA (target base 248–272), named siHO-2, was designed and synthesized by iGENE Therapeutics (Tsukubu, Japan), and scrambled ... HO-2 siRNA1 (target base 212–232) reported by other investigators [32], was also used GAPDH siRNA, named siGAPDH, was used as a control for siRNA When HeLa or HepG2 cells were 50% confluent, they...
Ngày tải lên: 19/02/2014, 05:20
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... incubated with or without organic selenium compound for at 37 °C in 35 mm glass-bottomed dishes (Matsunami, Osaka, Japan) After incubation, PMA was added to a final concentration of 100 ngÆmL)1 After ... Fukuta Y & Ishihara H (2001) Preparation of N,N-unsubstituted selenoureas and thioureas from cyanamide Tetrahedron Lett 42, 333–335 Koketsu M, Okayama Y, Aoki H & Ishihara H (2002) Facile synthesis...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... amino acid transporter Transporter cDNA (human) Transporter mRNA ⁄ GAPDH mRNA Vector Doxycycline + Name Gene symbol ETT ORCTL3 FLIPT1 EMT OCT1 SLC22A4 SLC22A13 SLC22A16 SLC22A3 SLC22A1 pEBTet pEBTet...
Ngày tải lên: 23/03/2014, 09:21
Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... hypoxia represents a decrease in O2 pressure in inspired gas and causes hypoxemia, which is a hemodynamic stress and could lead to pulmonary hypertension [23,24] Hypoxemia is a common manifestation ... each reporter construct and then incubated under normoxia or hypoxia for 48 h The two constructs, named HRESV40 and N-HRESV40, represent positive and negative controls for hypoxia Relative luciferase ... La Jolla, CA, USA), and then cloned into pCR-bluntII-TOPO (Invitrogen, Carlsbad, CA, USA), and named pCR-hHO1 and pCR-hHO2, respectively SP6 RNA polymerase was used for transcription of RNA probe...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo sinh học: "Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" doc
... also clustered to this group In addition, microRNAs of the 17~92 and paralog 106b~25 clusters (namely miR-19a, miR-19b, miR-20a, miR-25, miR-92, miR-93 and miR-106b) were identified among the ... miR-21 and miR-155 expression occurs upon in vivo differentiation of human CD8+ T lymphocytes Dynamic regulation of these microRNAs in mouse CD8+ T cells has been described upon in vitro activation ... http://www.translational-medicine.com/content/9/1/44 Page of 18 Neilson JR, Zheng GX, Burge CB, Sharp PA: Dynamic regulation of miRNA expression in ordered stages of cellular development Genes Dev 2007, 21:578-589...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học:" Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" pptx
... also clustered to this group In addition, microRNAs of the 17~92 and paralog 106b~25 clusters (namely miR-19a, miR-19b, miR-20a, miR-25, miR-92, miR-93 and miR-106b) were identified among the ... miR-21 and miR-155 expression occurs upon in vivo differentiation of human CD8+ T lymphocytes Dynamic regulation of these microRNAs in mouse CD8+ T cells has been described upon in vitro activation ... http://www.translational-medicine.com/content/9/1/44 Page of 18 Neilson JR, Zheng GX, Burge CB, Sharp PA: Dynamic regulation of miRNA expression in ordered stages of cellular development Genes Dev 2007, 21:578-589...
Ngày tải lên: 20/06/2014, 03:20
Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot
Ngày tải lên: 08/03/2014, 16:20
Báo cáo khoa học: Poly(ADP-ribose) polymerase-1 protects excessive DNA strand breaks from deterioration during repair in human cell extracts pot
... be interpreted as either PARP-1 dissociation or poly(ADP-ribosyl)ation However, crosslinking dynamics in the same reaction support the model in which PARP is being poly(ADP-ribosyl)ated first...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf
... Mem´ oria Samuel Solorzano Barruso References Manning G, Whyte DB, Martinez R, Hunter T & Sudarsanam S (2002) The protein kinase complement of the human genome Science 298, 1912–1934 Kamath RS,...
Ngày tải lên: 30/03/2014, 11:20
báo cáo hóa học:" Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines" pptx
... exciting new therapeutic option for malignant melanoma PhosphoSrc represents a promising pharmacodynamic marker for response to dasatinib and high levels of EphA2 may be a predictive marker for dasatinib ... inhibitor with potent antitumor activity in preclinical assays J Med Chem 2004, 47(27):6658-6661 Nam S, Kim D, Cheng JQ, Zhang S, Lee JH, Buettner R, Mirosevich J, Lee FY, Jove R: Action of the ... Palme H, et al.: Identification and validation of phospho-SRC, a novel and potential pharmacodynamic biomarker for dasatinib (SPRYCELtrade mark), a multi-targeted kinase inhibitor Cancer Chemother...
Ngày tải lên: 18/06/2014, 15:20
Báo cáo sinh học: " Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
... in alveolar and embryonal rhabdomyosarcoma Cancer Res 2006, 66:4742-4749 36 Puri N, Ahmed S, Janamanchi V, Tretiakova M, Zumba O, Krausz T, Jagadeeswaran R, Salgia R: c-Met is a potentially new ... of human melanoma Clin Cancer Res 2007, 13:2246-2253 37 Seiwert TY, Jagadeeswaran R, Faoro L, Janamanchi V, Nallasura V, El Dinali M, Yala S, Kanteti R, Cohen EE, Lingen MW, et al: The MET receptor...
Ngày tải lên: 18/06/2014, 19:20
Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
Ngày tải lên: 18/06/2014, 19:20
Báo cáo hóa học: " Expression of frog virus 3 genes is impaired in mammalian cell lines" pot
Ngày tải lên: 20/06/2014, 01:20
báo cáo hóa học:" Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
... in alveolar and embryonal rhabdomyosarcoma Cancer Res 2006, 66:4742-4749 36 Puri N, Ahmed S, Janamanchi V, Tretiakova M, Zumba O, Krausz T, Jagadeeswaran R, Salgia R: c-Met is a potentially new ... of human melanoma Clin Cancer Res 2007, 13:2246-2253 37 Seiwert TY, Jagadeeswaran R, Faoro L, Janamanchi V, Nallasura V, El Dinali M, Yala S, Kanteti R, Cohen EE, Lingen MW, et al: The MET receptor...
Ngày tải lên: 20/06/2014, 03:20
Báo cáo y học: " DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines" ppt
... have identified methylation QTLs genomewide, the majority of which act over very short distances, namely less than kb Furthermore, methylation patterns generally covary within individuals over distances ... to the human genome sequence (hg18) using BLAT [52] and MAQ [53] We selected 26,690 probes that unambiguously mapped to single locations in the human genome at a sequence identity of 100%, discarding...
Ngày tải lên: 09/08/2014, 22:23
Báo cáo y học: "LMP-420, a small-molecule inhibitor of TNF-alpha, reduces replication of HIV-1 and Mycobacterium tuberculosis in human cell" pps
... 76:11710-11714 Sha BE, Valdez H, Gelman RS, Landay AL, Agosti J, Mitsuya R, Pollard RB, Mildvan D, Namkung A, Ogata-Arakaki DM, Fox L, Estep S, Erice A, Kilgo P, Walker RE, Bancroft L, Lederman MM:...
Ngày tải lên: 10/08/2014, 05:20