atp dependent chromatin remodeling engines

... and (c) ATP- dependent chromatin remodeling complexes that reorganize nucleosomes (Sif, 2004) The ATP- dependent chromatin remodeling enzymes all belong to the SNF2 family of DNA -dependent ATPases, ... Vascular Remodeling 28 D Chromatin Remodeling Enzymes’ Roles in Development and Differentiation 31 i Introduction to Types of Chromatin Remodeling Enzymes 31 ii ATP- dependent Chromatin Remodeling ... proteins one through nine As an example of ATP- dependent chromatin remodeling, Brg1 and Brm1, the ATPase subunits of the SWI/SNF ATP- dependent chromatin remodeling complexes, will be briefly discussed...

Ngày tải lên: 24/08/2014, 11:38

... an ATP- dependent chromatin remodeling factor (reviewed in [25]) This ATP- dependent chromatin remodeling factor was found independently by two groups and was termed ACF (for ATP- utilizing chromatin ... H4 (ASF1 and CAF-1) and for H2A-H2B (NAP-1) ATP- hydrolysis is required for chromatin assembly, and may reflect a role for the ATPdependent chromatin remodeling factor ACF in the cell Linker histones ... it to DNA (C) ATP- dependent chromatin remodeling machines (such as ACF) are required in order to generate regular physiological spacing of nucleosomes in vitro In one report, the remodeling machine...

Ngày tải lên: 31/03/2014, 21:21

... to (A), except that the concentrations of ATP and the Me2+ (Mg2+, Ca2+, Mn2+, Zn2+) were both fixed at mM (C) EGTA-mediated reversal of (ATP ⁄ Mg2+) -dependent inhibition of peroxidase activity ... presence of 2.5 mm ATP These data provide the first evidence 1452 that the dual functions of 2-Cys Prx can be differentially regulated by ATP and Mg2+ The interaction with ATP modifies structural ... of F1 -ATP synthase J Biol Chem 276, 39505–39507 15 Wu G, Ortiz-Flores G, Ortiz-Lopez A & Ort DR (2007) A point mutation in atpC1 raises the redox potential of the Arabidopsis chloroplast ATP synthase...

Ngày tải lên: 18/02/2014, 17:20

... absence of ATP Relative activity (%) 100 82 56 20 in the absence of ATP under the conditions used These results indicated that the enzyme speci®cally degraded SulA3±169 in an ATP- dependent manner ... topologically quite different in the presence and absence of ATP Although the mechanism of ATP- dependent hydrolysis is not clear, ATP appears to somehow activate Lon protease so that the enzyme ... presence of ATP and 3-h incubation in the absence of ATP in this order Large, medium and small closed arrowheads indicate the fast, medium and slow cleavage sites in the presence of ATP (see Table...

Ngày tải lên: 21/02/2014, 03:20

... C, Rencurel C, Ferreira H & Owen-Hughes T (2003) Histone H2A ⁄ H2B dimer exchange by ATP- dependent chromatin remodeling activities Mol Cell 12, 1599–1606 11 Boeger H, Griesenbeck J, Strattan JS ... 55 56 glucocorticoid receptor during chromatin remodeling Mol Cell 14, 163–174 Krebs JE, Fry CJ, Samuels ML & Peterson CL (2000) Global role for chromatin remodeling enzymes in mitotic gene expression ... is included as a SWI ⁄ SNF-independent control The asterisk indicates the level of background with beads only Error bars indicate the values obtained from two independent cultures (C) Enrichment...

Ngày tải lên: 07/03/2014, 00:20

... inhibition of ATP- dependent ligases t t Vmax S L Oza et al ẵGSHẵSpd ATP abcKGSH KSpd KATP ẳ ẵGSH ẵSpd ATP ẵGSHẵSpd ẵGSH ATP ẵSpd ATP ẵGSHẵSpd ATP 1ỵ K ỵK ỵK ỵ cK ỵ ỵ ỵ bKGSH KATP aKSpd KATP abcKGSH ... inhibition of ATP- dependent ligases [GSH :ATP] mM 0.5:0.05 1/Rate (s) 1.2 0.8 0.75:0.075 1:0.1 0.4 1.5:0.15 2:0.2 0 1/[Spd] (mM1) Fig LineweaverBurk analysis of the variation of GSH and ATP at a xed ... of ATP- dependent ligases S L Oza et al 30 After correction for the background rate due to auto-oxidation of NADPH and hydrolysis of ATP in the coupling system, the net rates of endogenous ATPase...

Ngày tải lên: 07/03/2014, 04:20

... FEBS 433 Plant ATP- dependent phosphofructokinase 434 C Winkler et al FEBS Journal 274 (2007) 429–438 ª 2006 The Authors Journal compilation ª 2006 FEBS C Winkler et al Plant ATP- dependent phosphofructokinase ... archaeal, ATP- dependent 6-phosphofructokinase, an extremely thermophilic nonallosteric enzyme, from the hyperthermophile Desulfurococcus amylolyticus Arch Microbiol 173, 103–109 Plant ATP- dependent ... PPi-PFK might in fact correspond to ATP- dependent enzymes [40], experimental evidence to support this suggestion is lacking Here we report the purification of ATP- PFK from spinach leaves to electrophoretic...

Ngày tải lên: 07/03/2014, 11:20

... can be used to infer the presence of a functional Ser–Lys dyad in the proteolytic site )ATP +ATP )ATP +ATP 0 100 0 30 0 100 0 The crystal structure of the proteolytic domain of E coli Lon provided ... eight soluble proteolytic activities, one being ATP dependent Nature 292, 652 17 Goldberg, A.L., Moerschell, R.P., Chung, C.H & Maurizi, M.R (1994) ATP- dependent protease La (lon) from Escherichia ... Imanaka, T (2002) A membrane-bound archaeal Lon protease displays ATP- independent proteolytic activity towards unfolded proteins and ATP- dependent activity for folded proteins J Bacteriol 184, 3689–3698...

Ngày tải lên: 16/03/2014, 18:20

... poor ATPdependent duplex unwinding activity in vitro as shown previously for eIF4A [30] The unwinding in the absence of ATP is found significant, which is consistent with an intrinsic (ATP- independent) ... recombinant, LeIF protein from Leishmania infantum, and we demonstrate that it is an RNA -dependent ATPase and an ATP- dependent RNA helicase Sequence alignments show that LeIF is closely related to known ... vitro biochemical studies show that LeIF protein is an RNA -dependent ATPase that has the ability to unwind RNA ⁄ DNA heteroduplexes in an ATPdependent manner As is true of the other DEAD box proteins...

Ngày tải lên: 23/03/2014, 10:20

... yeast nuclear gene suv3 affecting mitochondrial post-transcriptional processes encodes a putative ATP- dependent RNA helicase Proc Natl Acad Sci USA 89, 6813–6817 Dziembowski A & Stepien PP (2001) ... helicase leads to premature aging disorder Werner syndrome); (b) the RFC4 protein, a DNA binding ATPase that acts as a processivity factor for DNA polymerase delta and epsilon and loads proliferating ... colonies were identified and subjected to sequencing Among 57 positive colonies 18 appeared to be independent clones and HBXIP (HBx interacting protein) proved to be the most frequently occurring...

Ngày tải lên: 23/03/2014, 15:21

... 2002 Chromatin remodeling in nuclear cloning (Eur J Biochem 269) 2285 N N A H1 B4 Stable chromatin Nucleoplasmin N N H1 B4 Less stable chromatin N B N TBP ISWI complex N N More relaxed chromatin ... diagrammatic examples of chromatin remodeling in Xenopus egg cytoplasm (A) Nucleoplasmin exchanges somatic linker histones H1 and H1° with egg linker B4 leading to less stable chromatin that is more ... basal transcription factor TATA binding protein (TBP) that is released from somatic chromatin by a chromatin remodeling protein complex containing ISWI, a member of the SWI2/SNF2 superfamily (Fig...

Ngày tải lên: 31/03/2014, 21:21

... data) Once the XPC– hHR23B complex binds to a DNA lesion of chromatin, some ATP- dependent chromatin remodeling factors may induce targeted chromatin reconfiguration around the lesion to assemble the ... presumably relies on the activity of one or more ATP- dependent chromatin remodeling complexes [50] Such activities may facilitate repair at different sites of chromatin by nucleosome movement, octamer ... analyses of NER in chromatin Results to date indicate that at least some, if not all, steps involved in NER requires active disruption of nucleosome structure by ATP- dependent chromatin remodeling complexes...

Ngày tải lên: 31/03/2014, 21:21

... and chromatin proteins Volume 377 includes genetic assays of histones and chromatin regulators, methods for the preparation and analysis of histone modifying and ATP- dependent chromatin remodeling ... Sequence Chromatin and Chromatin Remodeling Complexes By Rachel A Horowitz-Scherer and Christopher L Woodcock Recent progress in preparing and purifying defined sequence chromatin and chromatin remodeling ... Sequence Chromatin and Chromatin Remodeling Complexes By Rachel A Horowitz-Scherer and Christopher L Woodcock Recent progress in preparing and purifying defined sequence chromatin and chromatin remodeling...

Ngày tải lên: 11/04/2014, 01:17

... histones and chromatin regulators, methods for the preparation and analysis of histone modifying and ATP- dependent chromatin remodeling enzymes, and assays for transcription and DNA repair on chromatin ... and is important for transcriptional repression of certain SWI/SNF -dependent genes.84,85 The SWI/SNF ATP- dependent chromatin remodeling complex induces nucleosomal alterations that alleviate repression ... activation of the GAL1 gene is requirements from the same SAGA -dependent but Gcn5p-independent n,o chromatin- modifying complex Chromatin- modifying enzyme may Proteins related to yeast Gcn5p and...

Ngày tải lên: 11/04/2014, 01:19

... histones and chromatin regulators, methods for the preparation and analysis of histone modifying and ATP- dependent chromatin remodeling enzymes, and assays for transcription and DNA repair on chromatin ... visualizing chromatin and detecting chromatin interactions, enzymological assays for histone modifying enzymes, and immunochemical protocols for the in situ detection of histone modifications and chromatin ... variants, modified histones and defined chromatin segments, protocols for nucleosome reconstitution and analysis, and cytological methods for imaging chromatin functions in vivo Volume 376 includes...

Ngày tải lên: 11/04/2014, 02:02

... control regions show no open chromatin in this region similar to the B cell line GM-607 used as a negative control Taken all together our results suggest a potential chromatin remodeling process taking ... viral genes silencing Our experiments also demonstrate that these phenomena occur through chromatin remodeling and progressive closure of the CD3 genes promoter sites and are not the results ... migrated overnight at 50 V, and the radiolabeled protein complexes were detected by autoradiography Chromatin immunoprecipitation (ChIP) assay The ChIP assay was performed as previously described[28]...

Ngày tải lên: 18/06/2014, 18:20

... control regions show no open chromatin in this region similar to the B cell line GM-607 used as a negative control Taken all together our results suggest a potential chromatin remodeling process taking ... viral genes silencing Our experiments also demonstrate that these phenomena occur through chromatin remodeling and progressive closure of the CD3 genes promoter sites and are not the results ... migrated overnight at 50 V, and the radiolabeled protein complexes were detected by autoradiography Chromatin immunoprecipitation (ChIP) assay The ChIP assay was performed as previously described[28]...

Ngày tải lên: 20/06/2014, 01:20

... indicate that Tat recruits a component of the chromatin remodeling complex, BRG1, to the HIV-1 LTR Effect of acetylated Tat on recruitment of a chromatin remodeling complex The identification of BRG1 ... chromatin structure presents a significant barrier to transcription Various complexes, including HATs, covalently modify nucleosomal histone proteins through acetylation while ATP- dependent chromatin ... treatment [52] The disruption of nuc-1 occurred independent of transcription and was α-amanitin insensitive, suggesting that this chromatin remodeling was a pre-requisite for transcription, rather...

Ngày tải lên: 13/08/2014, 09:20

... what proteins interact with R3 methylation to achieve this remodeling Moved to expression Taking a step back from the analysis of chromatin remodeling at this level, Wendy Bickmore (MRC Human Genetics ... changes to DNA or the state of chromatin that have long-term influences on gene expression DNA methylation is considered a classic example of a repressive epigenetic chromatin mark In Xenopus embryos, ... and one chromatin remodeler, Fun30, a yeast homolog of the human protein SMARCAD1, contains these domains Overexpression of SMARCAD1 has been associated with genetic instability Fun30 has ATPase...

Ngày tải lên: 14/08/2014, 16:21

... activity (%) 100 * Pos ctrl 80 0.5mM ATP 60 1mM ATP * * 0.5mM ATP + 0.5mM 8-azido -ATP 40 ¤ 0.5mM ATP + 0.5mM 8-azido -ATP- B 20 ¤ 0.5mM 8-azido -ATP- B ¤ 0.5mM ATP + 0.5mM 8-azido-App(NH)p TP A o- ... ANP -dependent ATP binding on the NPR-A KHD S Joubert et al A Photolabeling 1250 * Ctrl + ANP + Ctrl ATP ANP ATP 1000 200 750 116 97 500 ¤ * 250 Ctrl ANP Ctrl + ATP ANP+ ATP B (-) (+) 97 kDa ATP ... et al ANP -dependent ATP binding on the NPR-A KHD 8-Azido-3¢-biotinyl -ATP inhibition of NPR-A GC activity To investigate the direct interaction of the NPR-A with ATP, we used 8-azido -ATP to which...

Ngày tải lên: 16/03/2014, 23:20