... separations of chiral compounds.[3, 6-8] Table 1.1 Common non-chromatography methods for chiral separation Category Separation of racemates by crystallization Chemical separation of racemates ... separation (C* denotes the chiral carbon) 24 Figure 1.9 Common configuration/view of quartz crystal microbalances with a holder and connector 28 Figure 1.10 AT- cut of a quartz ... crystallization Asymmetric transformation of racemates Formation and separation of diastereomer-resolving agents Separation via complexes and inclusion compounds Asymmetric transformation of diastereomers...
Ngày tải lên: 11/09/2015, 10:15
... used A number of methods have been attempted to bring proteins in an unsaturated state gradually into a supersaturated state The most commonly used method to crystallize proteins is the vapor ... precipitant Water diffuses from the drop to the reservoir solution leaving the drop with optimal crystal growth conditions The other methods include batch crystallization, micro-batch crystallization ... that a crystal is made up of similarly-shaped atoms that all have positive electron density, then there are statistical relationships between sets of structure factors These statistical relationships...
Ngày tải lên: 16/09/2015, 08:31
Structural basis of protein stability at poly extreme crystal structure of amya at 1 6 a resolution 3
... 3.1 The CD spectra of AmyA at different salt concentration The CD spectra monitored at 222 nm demonstrating the thermal denaturation of AmyA at different NaCl concentration 62 On the other hand, ... gel filtration chromatography Results from gel filtration chromatography with a Superdex-75 column at room temperature are consistent with the results from analytical ultracentrifugation In the ... any salt dependent oligomerization, we analyzed the quaternary structure of AmyA by analytical ultracentrifugation and gel filtration chromatography The sedimentation behavior of the AmyA protein...
Ngày tải lên: 16/09/2015, 08:31
Structural basis of protein stability at poly extreme crystal structure of amya at 1 6 a resolution 4
... be hydrated at low salt concentration This could be the physical explanation for the excess binding of calcium to AmyA at high salt The 77 coordination atoms to the calcium ions are tabulated below ... (http://pymol.org) Different atomic contacts were calculated using the WHAT IF program (Rodriguez et al, 1998) 70 Table 4.1 Crystal parameters and data collection statistics of hAmyA ... bridges are at different locations when compared to the salt bridges in lAmyA This difference is also to some extent due to the effect of crystal lattice formation as lAmyA and hAmyA crystals are...
Ngày tải lên: 16/09/2015, 08:31
Structural basis of protein stability at poly extreme crystal structure of amya at 1 6 a resolution 2
... incubated on ice for 30 minutes to increase the precipitation of heat denatured protein Any insoluble material was removed by centrifugation at 10,000g for 15 The supernatant was incubated at 65 ... proton donor protonates the glycosidic oxygen and the catalytic nucleophile attacks at C1 leading to formation of the first transition state The catalytic base promotes the attack of the incoming ... 1993) it was calculated that, in its native state at M NaCl, halophilic malate dehydrogenase (MDH) binds approximately 200 molecules of salt and almost 3000 molecules of water These values are...
Ngày tải lên: 16/09/2015, 08:31
A study of detection of enantiomers and chemical analogues by molecular imprinted polymer coated quartz crystal microbalance technique
... piezoelectric quartz crystal resonator is a precisely cut slab from a natural or synthetic crystal of quartz Quartz crystal in its perfect natural form can be seen in Fig.1.3 a) A quartz crystal microbalance ... resonator types depending on the cut angle with respect to the crystal lattice AT- cut crystals, which are predominately used for QCM devices, operate in the TSM and are Figure 1.4 AT- cut of a quartz ... desorption in MIP Figure 1.3 Schematic of a typical piezoelectric crystal Figure 1.4 AT- cut of a quartz crystal Figure 1.5 Schematic representation of a MIP-coated QCM sensor Figure 3.1 AFM images...
Ngày tải lên: 26/09/2015, 10:05
AN0826 crystal oscillator basics and crystal selection for rfPICTM and PICmicro® devices
... tuning CRYSTAL EQUIVALENT CIRCUIT We now look at how a quartz crystal operates internally and later we will see how they operate in crystal oscillators Understanding how the quartz crystal operates ... MCU oscillators generally operate below 30 MHz, which is the upper frequency limit of ATcut quartz crystals Series or parallel resonant? Colpitts Crystal Oscillator The Colpitts crystal oscillator ... capacitor) or crystal Initially, we will look at a few basic oscillator circuits that use a LC tuned circuit Later we will look at crystal basics and how crystal oscillators operate Figure shows...
Ngày tải lên: 11/01/2016, 14:27
Báo cáo khoa học: Crystal structures of the human SUMO-2 protein at 1.6 A and 1.2 A resolution ppt
... structure of human SUMO at high resolution by X-ray crystallography In this paper we present the crystal structure of a truncated SUMO-2 To facilitate crystallization, our strategy was to reduce ... using the SUMO-2/pET28a as a template The PCR was carried out for 25 cycles of 30 s at 95 °C, 30 s at 55 °C and 30 s at 72 °C, using two primers 5¢-GGAATTCCATATGGGAGTCAAGACTGAGAA CAAC-3¢ and 5¢-CCGCTCGAGTCAACCTCCCGTCT ... W.-C Huang et al (Eur J Biochem 271) Crystallization and data collection Crystallization was achieved by the hanging-drop vapour diffusion method at room temperature using the CryoII screen kits...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: ˚ Crystal structure at 3 A of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose pdf
... protein solution at 18 mgÆmL)1 concentration contained 0.1 M galactose and 0.01 M acetate buffer at pH 4.0 The reservoir solution contained 0.9 M ammonium sulfate and 0.1 M glycine buffer at pH 3.4 ... Hexagonal crystals grew to about 0.2 mm in a few weeks Sequence data were obtained as described previously [24] and in [27] The X-ray data were collected at the Synchrotron Radiation Source (SRS) at ... a glycosylating MLA sugar at Asn112 was seen to occupy (at least partially) the guanine-binding site of the RTA substrate analogue However, glycosylation appears not to affect the catalytic activity...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo y học: "Can ultrasonography make identification of asymptomatic hyperuricemic individuals at risk for developing gouty arthritis more crystal clear" pptx
... trials of patients with proven MSU crystal gouty arthritis are needed to further evaluate the use of US to predict the presence of asymptomatic gouty arthritis in an individual hyperuricemic patient ... hyperuricemic patient Abbreviations MSU, monosodium urate; NHANES, National Health and Nutrition Examination Survey; US, ultrasound Competing interests The author declares that she has no competing ... acute flares will influence our decision of when to initiate and commit to a long-term urate-lowering therapy and chronic antiinflammatory treatment is still to be determined US may serve as a noninvasive...
Ngày tải lên: 12/08/2014, 15:22
(Crystal Report – Tạo báo cáo)
... Delphi truyền vào Lưu ý, chạy report Crystal, trường param hỏi giá trị, ta cần nhập vào Crystal để hiển thị tạm thời - Special fields: trường đặt biệt có sẵn Crystal số trang, trang thứ mấy, ngày ... qua lại tab design tab preview - • lặp • Các format Suppress Suppress If Duplicated - Suppress : Hiển thị đối tượng hay không - Suppress If Duplicated : Không hiển thị đối tượng có trùng Tips - ... footer - Hướng dẫn thực hành Nhập môn CNPM Field Explorer Khi chọn xong đối tượng database cho vào report, Crystal cửa sổ Field Explorer Nếu không thấy cửa số Field Explorer, ta click vào button...
Ngày tải lên: 28/08/2012, 11:13
Công cụ Cut và Link file
... pos('\',name)0then repeat delete(name1,1, pos('\',name1)); until pos('\',name1)=0; assign(f,name);reset(f,1); write(' Hay cho dia moi roi an Enter de tiep tuc ');readln; writeln; repeat str(sof,extname); ... sûã dng Chẩy chûúng trònh bùçng cêu lïånh sau tẩi dêëu múâi ca DOS: Trang VIETBOOK ·1 CUTF_tïn tïåp cêìn Cut_ tïn ưí àơa mïìm: Dêëu _ úã trïn àûúåc sûã dng àïí tûúång trûng cho dêëu cấch sûã dng ... bam Enter de tiep tuc ');readln; writeln; sof:=sof+1; end; until eof(f); close(f); writeln('Da cat xong file:',name); wrireln('Tong cong chia ra:',sof+1,'file.'); END Dõch chûúng trònh vûâa soẩn...
Ngày tải lên: 06/09/2012, 14:25
Game Theory at Work - James Miller
... of your boats By destroying your boats, you limit your choices You can no longer take the easy way out of the battle by retreating Eliminating the option of retreating makes your threat credible ... fissionable materials could be challenging An easier method is to threaten to match any price cut If your rival considers cutting prices to steal your customers, then his justification for a price cut ... stupid A strictly stupid strategy is a strategy that gives you a lower payoff than at least one of your other strategies, regardless of what your opponent does Knowing that your opponent will never...
Ngày tải lên: 03/10/2012, 15:19
Hệ thống lập trình điều khiển máy cắt HL Wire Cut System ( Pci bản)
... số gia công sau: Cài đặt tham số gia công: V.F Biến tần Offset giá trị bù G rade Giá trị độ côn Ratio Tỷ lệ gia công Axis Chuyển đổi tọa độ Loop Gia công tuần hoàn Speed Bước tốc độ XYUV đẩy chỉnh ... riêng) Có hai điểm cần phải ý: 4.1 Khi lập trình đĩa lưu liệu đĩa lưu trình tự , liệu hình vẽ XX DAT liệu XX 3B lệnh 3B lưu vào đĩa ảo, mà đĩa ảo tắt máy sau khôi phục không bảo lưu, cần phải lưu ... hình vẽ, ổ cứng , đĩa mềm, ấn nút trở lại máy 4.2 Khi lập trình, Nếu muốn lôi liệu hình vẽ ( XX.DAT) lưu kho hình vẽ, ổ cứng , đĩa mềm để dùng trước hết để liệu hình vẽ điều chỉnh nhập vào đĩa...
Ngày tải lên: 24/10/2012, 16:56
Báo cáo y học: "Effects of Losartan on expression of connexins at the early stage of atherosclerosis in rabbits"
... the formation of the atherosclerotic plaque Previous study demonstrated that rats lack of Cx43 expression showed 50% lower rate of attack with atherosclerotic plaque compared with normal rats (9) ... with hematoxylin Western blot Protein extracts were prepared in lysis buffer and then repeatedly aspirated (20 times) through a 23-gauge needle followed by centrifugation at 4°C for 30 at 10,000 ... AngII, whether up-regulation of Cx43 can be antagonized by AT1 antagonists or whether suppressed smooth muscle cell proliferation and migration by AT1 antagonists are mediated through decreasing...
Ngày tải lên: 26/10/2012, 09:39
Báo cáo y học: "Mechanical complications and reconstruction strategies at the site of hip spacer implantation"
... Mechanical complications and reconstruction strategies The exact rate of mechanical complications following hip spacer implantation remains unknown Despite numerous reports about these complications, only ... on the operated extremity, the patient should be rather considered as a candidate for a resection arthroplasty and not for a spacer implantation For prevention of any spacer dislocation due to ... data available that have demonstrated that the insertion of a metallic endoskeleton significantly improves the mechanical properties of hip spacers or reduces the rate of mechanical complications...
Ngày tải lên: 26/10/2012, 09:53
báo cáo tiếng anh thương mại Machinery import activities at Việt NamPhat Co., Ltd Situation and suggestions.doc
... international cooperation, the Vietnamese government has allowed various business types, including state-owned enterprises, jointventures, business cooperation and private sector to participate ... specified in the import contracts), international business practices and international conventions, treaties that the parties participate in The laws and regulations of the exporting country can make ... at No 37/4, Phuongmai Road, Hoangmai, Hanoi A representative office was opened at No 21, 152 A Caolo Road, Ward, District, Hochiminh City in 2005 In september 2005, VNPhat Co., Ltd was relocated...
Ngày tải lên: 27/10/2012, 16:40