association analysis of candidate gene polymorphisms

... Family-based association study with DNA markers of the ENPP1 locus We selected three STR and four SNP markers of the ENPP1 locus to obtain a fairly complete coverage of the gene region (Fig 2) Analysis of ... Ginter EK: Population genetic study of the Alatyr Region of the Republic of Chuvashia Genetica 2002, 38:291-298 Livshits G, Karasik D, Kobyliansky E: Complex segregation analysis of the radiographic ... 3.2% of the population variation of FS1-OA The contribution of the whole PPi pathway to the genetic component of the disease development may be considerably larger To better understand the role of...

Ngày tải lên: 09/08/2014, 06:23

... dehydrin genes, part of the COR gene family, are regulated by the Cbf gene family and the Dreb2 gene via the cis-element DRE/CRT present in the promoter region of COR genes [19] Transcripts of HvDhn1, ... expression profiling, exploitation of the allelic and phenotypic variation of FT in rye by association studies has lagged behind [20,21,24] This study reports the first candidate gene- based association ... binding specificity of the Cbf gene family Effect sizes of markers, commonly expressed as percentage of the genetic variance explained by markers, are of primary interest in association studies...

Ngày tải lên: 11/08/2014, 11:21

... DEPARTMENT OF EPIDEMIOLOGY AND PUBLIC HEALTH YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNVERISTIY OF SINGAPORE Association Analysis of Genetic Variation of Estrogen Related Candidate Genes in Breast ... further explore the association between genetic variants in ESR2 and breast cancer risk 7.5 Candidate gene based genetic association study Candidate genes are chosen in genetic association studies ... of ER positive breast cancer Study III: Association analysis between genetic variants in ER cofactor genes and endometrial cancer risk The main objectives of these studies were: To identify genetic...

Ngày tải lên: 09/09/2015, 17:58

... frequencies have been shown to be useful as genetic markers for genetic association studies We selected SNPs in the human CYP19A1 gene as markers for the genetic association experiment (Fig 1) All SNPs ... Japanese individuals Figure Organization of the human CYP19A1 gene and location of SNPs The gene is approximately 130 kilobase pairs (kb) in length, and has a total of 11 exons Boxes indicate exons, ... deletion of the aromatase gene, exhibit reduced blood pressure (BP)[3] Thus, we hypothesized that aromatase is one of the factors affecting BP, and that the aromatase gene is a susceptibility gene...

Ngày tải lên: 08/08/2014, 16:23

... position of the gene Each of these SNPs had a minor allele frequency ≥ 19% Using the Haploview program [26], three of the five single nucleotide polymorphisms (SNPs) of the interleukin 13 gene were ... http://www.aacijournal.com/content/6/1/6 Page of 13 Table Multivariate analysis of the association of elevated total serum IgE at age 10 (>200 kU/L) with IL13 polymorphisms and prenatal factors, stratified ... derived from a combination of family income, parental occupation (socioeconomic status), and number of children in child’s bedroom Table Multivariate analysis of the association of inhalant serum IgE...

Ngày tải lên: 08/08/2014, 21:20

... that the stimulated PBMC are a source of mRNA for the target genes of interest The expression of other relevant genes, including IL-4, IL-10 and IFN-γ, generated satisfactory standard curves ... order to obtain homogeneity of variance Results Development of standard curves to correct for variable PCR efficiency One of the primary problems encountered using PCR to quantify gene expression ... influence, the CE values of the target genes are normalized to a reference gene using the CE for GAPDH The RE compared with GAPDH is determined by dividing the CE of the target gene by the CE for GAPDH...

Ngày tải lên: 09/08/2014, 01:23

... carriers of three or more risk alleles Page of Discussion In the present study, we examined for the first time the contribution of three polymorphisms in the promoter region of the IL18 gene for ... eNOS polymorphisms, in Italians [24,28] This evidence may indicate a Page of high reproducibility of the genetic associations with GCA among different populations and that the potential association ... Lackner K, Cambien F, Blankenberg S, Athero Gene Investigators: Genetic analysis of the interleukin-18 system highlights the role of the interleukin-18 gene in cardiovascular disease Circulation...

Ngày tải lên: 12/08/2014, 12:20

... combination of alleles at the same three functional polymorphisms [4] Other studies have left this matter more or less undefined because of the lack of convincing evidence of the relevance of all the polymorphisms ... from the combination of two functional polymorphisms, rs2004640 and rs10954213, and a SNP of unknown relevance [3], or from a gradation of the effects of three functional polymorphisms, the two ... not find any significant effect of this SNP or of any of the functional polymorphisms [26] These contrasting pieces of evidence not allow for a clear understanding of IRF5 cis-regulation and its...

Ngày tải lên: 12/08/2014, 15:23

... TSS of all the genes in a gene battery (or their orthologs in other species) The same procedure was also applied to a set of 2,000 random genes of the same species analyzed The TSS of a gene ... Assessment of the ancestral cores Assessment of the ancestral cores For each gene battery we show the probability of the genes to be part of the ancestral core for lineage b given that the genes are ... al R172.4 Figure (see previous page) Conservation of the gene batteries Conservation of the gene batteries (a) Conservation profiles of the gene batteries For each battery, the over-represented...

Ngày tải lên: 14/08/2014, 21:20

... genome-wide study of this gene family I undertook a comprehensive phylogenetic analysis of this gene family to determine how it evolved in A queenslandica and other sponges This analysis is presented ... exception of L oparinae (five gene sequences) and A queenslandica (six genes) In A queenslandica, the different numbers of cathepsin L genes are probably a consequence of species-specific gene duplication ... genomic organisation of A queenslandica silicatein genes…… 16 2.4.2 Conservation in the genomic structure of A queenslandica silicatein genes……… 20 2.4.3 Phylogenetic relationship of sponge silicateins……………………………………………………23...

Ngày tải lên: 13/10/2015, 14:07

... course of my research programme at the National University of Singapore i TABLE OF CONTENT Pages Acknowledgements i Table of contents ii Summary v List of Tables vi List of Figures vii List of Abbreviations ... MOLECULAR ANALYSIS OF THE GENE LAS17 MEDIATING T-DNA TRAFFICKING IN YEAST CELLS HARIPRIYA BATHULA (B.pharm, M.Tech) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL ... range of the Agrobacterium S cerevisiae is also the often used eukaryotic model organism by many scientists because of its ease of handling, the availability of vectors, and also the ease of transformation...

Ngày tải lên: 13/10/2015, 16:50

... Biology 2008, 8:7 Background Association genetics via LD mapping is an emerging field of genetic mapping that has the potential to reach resolution to the level of individual genes (alleles) underlying ... potential of automatic high-throughput analysis make them ideal candidates as molecular markers for construction of highdensity genetic maps, QTL fine mapping, marker-assisted plant breeding and genetic ... crop genetic improvement [18-22] Association analysis includes the estimation of nucleotide diversity, the assessment of linkage disequilibrium structure (LD) and/or the correlation between polymorphisms...

Ngày tải lên: 12/08/2014, 05:20

... mannose-binding lectin gene polymorphisms Polymorphisms in codons 54 and 57 of the MBL gene were analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) Briefly, for determination of polymorphisms ... polymorphism in codon 57 of the MBL gene Discussion MBL is one of the most important constituents of the innate immune system [10,11] Many studies have reported a deficiency of MBL in different populations ... Roberton D: Restricted polymorphism of the man- Page of (page number not for citation purposes) 21 22 nose-binding lectin gene of indigenous Australians Hum Mol Genet 2000, 9:1481-1486 Carty SM,...

Ngày tải lên: 09/08/2014, 08:22

... the association, previously reported in European Caucasians, of TXNDC3 with knee OA susceptibility in an East Asian population Table Meta -analysis: summary of association and heterogeneity of ... differences in the association of ASPN, CALM1, COL2A1, COMP, and FRZB with genetic susceptibility to osteoarthritis of the knee Arthritis Rheum 2007, 56:137-146 Ikegawa S: New gene associations in ... Carr A, Slagboom PE: Genetic association analysis of RHOB and TXNDC3 in osteoarthritis Am J Hum Genet 2007, 80:383-386 Kellgren JH, Lawrence JS: Radiological assessment of osteoarthrosis Ann...

Ngày tải lên: 09/08/2014, 10:23

... course of T cell mediated immune responses, are prime candidates for study in conditions where there is an imbalance in the T helper cytokine profile Polymorphisms in a range of human cytokine genes ... This suggests that this region of the gene could be of vital importance in the regulation of IFN-γ gene expression Although in the current study we did not observe an association between the polymorphic ... study we evaluated the distribution of single nucleotide polymorphisms in the 3'UTR region of two important candidate gene – IL-12 and IFNγ-but found no direct association with susceptibility to...

Ngày tải lên: 13/08/2014, 13:20

... associated with allergy and asthma [29] Candidate genes such as a cluster of cytokine genes (interleukins 3, 4, 5, 9, 13 and the β-chain of the IL-12 gene) , CD14 gene and genes coding for the corticosteroid ... nucleotide polymorphisms (SNPs) in candidate genes influencing the clinical expression of asthma and atopy (Table 2.1) To add to the complexity, the interaction of these genes and polymorphisms ... As some of these markers flank candidate cytokine genes, we postulate that polymorphisms found in the promoter or within the IL-12 and IL-13 genes as well as polymorphisms in the CD14 gene may...

Ngày tải lên: 30/09/2015, 14:23

... of genes warranting further scrutiny In order to obtain an expression profile of the genes which changed the most with age, we uploaded all of our array data into the array analysis program of ... Expression profile of the most significantly changed genes with age This profile was generated in the NHGRI website (https://arrayanalysis.ni h.gov), using distance-based analysis of total data ... levels of many of these genes identified in the array analysis Moreover, Western blot analysis of thymocyte lysates also confirmed that IgM increases with age (Figure 3) Flow cytometry analysis...

Ngày tải lên: 03/11/2012, 11:35

... (Qiagen, California, USA) Quantification of 16S rRNA gene and C12O gene and C23O gene by MPN-PCR The copy numbers of 16S rRNA gene, C12O gene and C23O gene in each bacterial community were measured ... PCR-DGGE banding patterns of 16S rRNA gene, C12O gene and C23O gene in test system A were notably different from those in test system C The PCR-DGGE profiles of C12O gene and C23O gene showed a relatively ... number of frond of S polyrrhiza in test systems A and B × 300 mL water in test system C or MPN-DNA copies mL PCR-DGGE analyses of 16S rRNA gene, C12O gene and C23O gene PCR-DGGE analyses of 16S...

Ngày tải lên: 05/09/2013, 10:15

... success of both the DNA elution and re-amplification depends on the amount of cDNA generated during the first round of PCR A good way of optimizing the first round PCR is to take advantage of known genes ... scintillation counting of isolated products on sections of the filter Virtual Northern blotting Semi-quantitative analysis of gene expression profiles, either by Northern blot analysis or by differential ... the intensity of the signals in each band by using appropriate software, often supplied by the manufacturer of the imaging equipment These programs allow integration of the intensity of the band...

Ngày tải lên: 25/10/2013, 22:20

... locus at any age of the animal irrespective of sex and eliminates the need for the milk of lactating females Fig.1 Agarose gel electrophoresis of PCR products of beta-lactoglobulin gene in Murrah ... and were sequenced in Genei PCR amplification and RFLP analysis of b-lg locus of 110 Murrah buffaloes revealed no polymorphism in the present study The DNA typing results of this study agreed ... of each primer,2.5 units of Taq.DNA polymerase,1X PCR buffer(10mM Tris – HCL, pH 9.0, 50 mM KCL and 1.5 mM MgCl2), 150 mM of each dNTP and 50 ng of genomic DNA After an initial denaturation of...

Ngày tải lên: 18/02/2014, 02:20