assays using synthetic or dyed substrates

... that an array of minor modifications forms the structural basis for enhanced stability in thermophilic xylanases Therefore, several factors, which are thought to be responsible for thermostability, ... [30] using a 1.4 A ˚ probe Van der Waals volumes were calculated using 1.4 A probes and without probes with the VOIDOO program [31] Numbers of hydrogen bonds were calculated for all xylanases using ... xylanases According to Torronen et al [8], the shape of the molecule resembles a ¨ ¨ right hand: two b-sheets and the a-helix form fingers and a palm, a long loop between the B7 and B8 strands forms a...

Ngày tải lên: 20/02/2014, 23:20

... THE RESULTS Contents Introductory Tutorial for SAP2000 Introduction Using This Manual Overview of the Program 1-2 Using this Tutorial 1-1 1-2 An Introductory Tutorial The Project 2-2 The Interface ... Introductory Tutorial C Click the drop-down box that reads “Import I/Wide Flange” in the Choose Property Type for Add area of the form Scroll down the list of import options until you find Import ... the deformed shape shown in Figure 33 Figure 33 Deformed Shape in an elevation view Figure 32 Deformed Shape form Step Graphically Review the Analysis Results - 33 Introductory Tutorial for SAP2000...

Ngày tải lên: 06/09/2012, 15:56

... can be exported to and imported from AutoCAD and other programs that use DXF, IFC, or IGES file formats Similar import/export capabilities are available for the ProSteel and FrameWorks Plus packages ... model supports or other localized behavior o Grounded (one-joint) link objects: Are used to model special support behavior such as isolators, dampers, gaps, multi-linear springs, and more Line ... in the interface, or exported to a Microsoft Access database file, a Microsoft Excel spreadsheet file, or a simple text file Data can be exported to create reports or to perform specialized calculations...

Ngày tải lên: 01/04/2014, 03:20

... individual isotropic B-factors followed Water molecules were either automatically identified by program CNS or manually introduced using program O The final refinement was performed using the TLS approach ... ¨ initializing 350-bp fragment and C Vonrhein for help with SHARP/ AUTOSHARP Moreover, we are grateful for the contributions of S Thorspecken, A Dorowski, B Phillips, S Jelakovic, C Schleberger ... conformational activation energy barrier during CD hydrolysis Acknowledgements We thank H Bender for drawing our attention to the enzyme, E Schiltz for amino-acid sequence analyses, M Ruckels for...

Ngày tải lên: 08/03/2014, 02:20

... (1989) Molecular Cloning: a Laboratory Manual, 2nd edn Cold Spring Harbor Laboratory Press, New York 36 Navaza, J (2001) Implementation of molecular replacement in AMORE Acta Crystallogr D Biol ... potential solution Euler angles, fractional coordinates, correlation coefficients and R factors for the best molecular replacement solution for each model, using P41212 as the space group of the 1Q9H ... solutions had correlation coefficients of 56.1% and 55.3%, and R-factors of 40.0% and 40.9% for 1CEL and 1GPI, respectively Refinement of the models produced by AMORE was performed by using the CCP4...

Ngày tải lên: 23/03/2014, 13:20

... – – – – – 12% – – – – – – – – [55] 96% 37% 85% 44% 84% 54% 86% Normal ND Normal ND Normal Normal ND – – – Altered Normal Normal Normal Altered Altered Altered – – – 55% 23% 123% 36% 45% 77% 12% ... with respect to (A) (the NH2-terminus is omitted for clarity) Cavities are colored by atom type (red stands for O, blue for N and yellow for S) The horizontal black lines delineate the approximate ... (a) Normal ND ND – Normal (a) Normal (a) Normal Normal ND ND – – – Misfolded ND – – We thus see that the pathway outlined by the cavities numbered 7–10 connects the bottom of the phosphorylation...

Ngày tải lên: 23/03/2014, 21:20

... a function of the longitudinal direction using NSOM has not been reported According to numerical studies [11] on bowtie ridge nanoaperture optical performance, the focused spot is a near-field ... surface because the forces used for feedback are fundamentally shortrange Another widely used method is the constant-height scan (CHS) [16,17], which is a simpler method for three-dimensional ... overcome tip-sample attractive force (around −80nN in Fig and −140nN in Fig 6) The force difference in Figs and is likely caused by the worn-out NSOM tip This attractive force will bend the NSOM cantilever...

Ngày tải lên: 06/05/2014, 08:54

... a function of the longitudinal direction using NSOM has not been reported According to numerical studies [11] on bowtie ridge nanoaperture optical performance, the focused spot is a near-field ... surface because the forces used for feedback are fundamentally shortrange Another widely used method is the constant-height scan (CHS) [16,17], which is a simpler method for three-dimensional ... overcome tip-sample attractive force (around −80nN in Fig and −140nN in Fig 6) The force difference in Figs and is likely caused by the worn-out NSOM tip This attractive force will bend the NSOM cantilever...

Ngày tải lên: 06/05/2014, 08:58

... When n is of the form j or j + l2 , j + l(l + 1), the transformation between c6 and c7 was also effectless; when n is of the form j (j + 1) or j (j + 1) + l2 , j (j + 1) + l(l + 1) or j(j + 1)2 , ... Lemma 2.12 14 For n of the form l2 or l(l + 1), we have { c ∈ Mn−1 : q− (Mn , c) = } = Sn−1 , { c ∈ Mn+1 : q+ (Mn , c) = } = Mn+1 Lemma 2.13 For n not of the form l2 or l(l + 1), we have { c ∈ ... planar three dimensional polyomino with normal vector ei We simply transform the unit squares into unit cubes and rotate the polyomino so that its normal vector becomes ei This trick will be used...

Ngày tải lên: 07/08/2014, 06:20

... images or as movies, but they are much more informative if they can actually be manipulated interactively in 3D The use of the VRML97 standard file format for interchanging 3-D models via the World ... solution were added and the chloroform-containing phase was collected After evaporation of the chloroform and dissolving of the lipids in isopropanol, spectrofluorometric readings were taken with ... including relative weightings for the external and internal forces and for additional 'pressure' forces; a choice between normal image gradient and 'gradient vector flow'; desired vertex spacing,...

Ngày tải lên: 11/08/2014, 00:22

... which was positive for AuNPs and negative for organelles, as shown in Figure 6(d) AuNPs in this grey image were easily identified using computer-based image analysis In this work, we developed ... and the diffusion kinetics for the recruitment of receptors to the binding site The other is the thermodynamic driving force for wrapping The thermodynamic driving force refers to the amount of ... images of AuNPs and cellular organelles were mapped by using a dark-field optical sectioning microscope [15] For long-term observation of Figure The scattering spectra for different nanoparticles...

Ngày tải lên: 11/08/2014, 00:22

... Written informed consent was obtained from the patient for publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief ... Germany Authors’ contributions PM analyzed and interpreted patient data regarding the cardiac disease and was a major contributor in writing the manuscript PM performed the transthoracic echocardiography ... fulminant PE One patient is our own case report The other report describes an extensive right atrial and ventricle thrombus formation encircling a temporary pacing wire in a patient with heparin-induced...

Ngày tải lên: 11/08/2014, 02:22

... to be mm or less for lateral translations and 8.4 mm or less for forward translations The MADBO values were 3.5° or less for flexion-extension rotational measurements and 1.9° or less for all axial ... fixed or random factor and (b) the type of error included (true score variance, systematic and /or random) [28] In the equation for calculating ICCs, this changed the denominator [20] For the ... numerical rating scores (NRS) for pain and Short Form questionnaire (SF36) scores indicated a near normal group (Tables and 2) We noted that for four variables (RyHead, RyThorax, RyPelvis, and...

Ngày tải lên: 13/08/2014, 14:20

... chest wall, the pectoralis major was retracted, and the pectoralis minor bluntly dissected from its origin from about the 2nd to the 5th rib Using a blunt probe, the inferior border of the 3rd interspace ... Transgene expression of fluorescent reporter proteins is not limited to single color applications The Lichtman laboratory has developed an elegant system of targeting fluorophore combinations to subpopulations ... photon absorption must be essentially simultaneous for effective fluorophore excitation This would have too low a rate of occurrence to be exploitable for fluorescence microscopy In order to augment...

Ngày tải lên: 24/08/2014, 12:57

... isolator with biaxial plasticity behavior • Base isolator with friction and /or pendulum behavior • SAP2000 • AVI file option for creating real time displays of nonlinear deformation behavi or Frame ... Fully formatted, customized reporting • DXF Import and Export Link to AutoCAD Key Features SAP2000 Interactive Modeling Powerful Modeling Capabilities Interactive Modeling SAP2000 • Work on Multiple ... excitation Sequential history cases Time history Windows AVI file Results can be combined with other loads for enveloping or step by step steel and concrete design Time History Analysis Analysis...

Ngày tải lên: 11/06/2014, 03:16

... first kind of order m Jm Bessel function of the first kind of order m kn wave number of the n-th mode L wave length Ls Smagorinsky length scale m normal vector m ˜ unit normal vector for the new ... the generation and transport of vorticity and turbulence cannot be investigated based on potential flow theory In order to obtain the turbulence and vorticity transport information as well as the ... operation of offshore structures (eg Floating Production Storage Offloading (FPSO) vessels or very large floating airports) in extreme waves, the stability of offshore structures such as spar platforms subjected...

Ngày tải lên: 13/09/2015, 20:56

... would like to thank our collaborator, Dr Glenn Prestwich, from the University of Utah, for the generous gift of his material, technical and advisory support His work and insights were critical ... introduced into this mesh by the incorporation of bioactive growth factors vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF-BB) and bone morphogenetic proteins (BMP-2) ... separation (TIPS) are straight forward and widely reported methods for fabricating scaffolding structures[18] Unfortunately, these methods lack precise control over porosity, pore size and architectural...

Ngày tải lên: 14/09/2015, 08:24

... forms for more practical purposes Keywords: Metamaterials, left-handed, S-shaped resonators, three-dimensional, freestanding, terahertz Thesis supervisor: MOSER Herbert Oskar Title: Professor ... performed a systematic theoretical study of such materials and had coined the word ―left-handed‖ for such class of materials due to the left handed triad formed by the electric field vector vector ... ―left-handed‖ for such media due to the left-handed triplet formed by the electric field intensity vector , magnetic field intensity vector and wave vector [1] The Poynting vector ( = x ) maintains...

Ngày tải lên: 06/10/2015, 20:42

... 5, 2010, pp.757-768 heat transfer past a vertical porous plate through a porous medium under oscillatory suction and heat source The study reported herein analyzes the effect of periodic suction ... flow of a viscous incompressible fluid past an infinite vertical porous plate embedded in a porous medium The governing equations for the velocity and temperature of the flow field are solved employing ... tables Mathematical formulation of the problem Consider the three dimensional flow of a viscous incompressible fluid past an infinite vertical porous plate embedded in a porous medium in presence...

Ngày tải lên: 05/09/2013, 14:58