assay based on detection of dyed oligosaccharides produced by hydrolysis

... conjunction with Pitch Determination Algorithms (PDA) in which the V/UV decision is made as part of the pitch determination problem, whereas the second includes solutions based on the value of ... robust V/UV detection based on adaptive noise classification and SNR estimation In this paper we present an extended version of this work Specifically, the performance of the classification system ... stationary unvoiced, nonstationary unvoiced, onset, nonstationary voiced, or stationary voiced A multilevel approach is used for the classification decision, starting with a VAD, followed by several...

Ngày tải lên: 21/06/2014, 20:20

... case of OSCC both the tests gave concordant result with an overall level of agreement of 88.3% (Cohen’s Table Concordance of results of HC-II assay and PCR test for detection of HPV infection in ... value (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 is negative for HR HPV; Cutoff ratio greater ... (22.7%) non chewers, 20/141 (14.2%) non smokers, 21/141 (14.9%) non drinkers were negative On the basis of location of the lesions in potentially malignant OSMF of the oral cavity, out of 42 positive...

Ngày tải lên: 12/08/2014, 01:22

... Determination of the HAV neutralization potency of human IG preparations by ARTA Determination of the HAV neutralization potency of human IG preparations by ARTA HAV-Bsd was treated with 1% of Lab ... http://www.virologyj.com/content/5/1/155 Figure Determination of the HAV neutralization potency of human IG preparations by ELISA Determination of the HAV neutralization potency of human IG preparations by ELISA ... at 4°C followed by h incubation at 37°C Four-fold dilutions of the neutralization reactions were titrated on 96-well plates containing Huh7-A-I cell monolayers After h adsorption at 35°C, cells...

Ngày tải lên: 12/08/2014, 04:21

... the initiation, promotion and progression of many diseases - 27 - such as cancer, tissue injury and inflammation (Floyd 1990) 8-OH-Gua has been commonly detected and identified by one of the already-existing ... chemicals These strains were kindly supplied by Dr T Nohmi of the National Institute of Health Sciences S9 fraction of phenobarbital/5,6-benzoflabone-pretreated male Sprague-Dawley (SD) rat liver ... 233-246 Yamada, M., Sedgwick, B., Sofuni, T and Nohni T (1995) Construction and characterization of mutants of Salmonella typhimurium deficient in DNA repair of O6-methylguanine, J Bacteriology,...

Ngày tải lên: 05/09/2013, 08:40

... Immunodetection of the yeast mitochondrial proteins was carried out with monoclonal and polyclonal antibodies by chemiluminescence The stained polyacrylamide gels and the fluorographs containing ... Protein concentrations were determined by the Bradford method [26] or the modified Lowry method [27] Electrophoretic analysis of DNA on agarose gels, restriction endonuclease analysis, ligation of DNA ... phenotype of the yeast strains with deletions of genes encoding various subunits of the bc1 complex was determined by plating the cells on solid media containing fermentable or nonfermentable carbon...

Ngày tải lên: 19/02/2014, 12:20

... three-dimensional structure of the isolated SBD alone has been determined by NMR as well as by X-ray crystallography of enzymes that contain this SBD [32–38] The CBM20 module consists of seven b-strand ... sequences of both families separately, taking into account the above-mentioned lack of structure–function information concerning CBM21 This was followed by attempts to identify the CBM20 sequence of ... evolutionary tree This provides a basis for the joining of the two CBMs into a common clan Results and Discussion Location of SBD modules in CBM20 and CBM21 With regard to the location of the...

Ngày tải lên: 07/03/2014, 21:20

... alterations occurring at the key transition from hyperplasia to dysplasia include loss of expression of MLH1 in group CRC and loss of expression of MGMT and ⁄ or aberrant expression of p53 in ... adenomas of the colorectum.109,127 By contrast, secretory mucin production in conventional colorectal adenomas is decreased, leaving expression of the transmembrane glycoprotein MUC1 only in areas of ... widely accepted notion that other components of the ‘canonical’ Wnt pathway can be invoked in the initiation of the subset of CRCs without APC mutation is unproven presence of unexp ect ed genetic...

Ngày tải lên: 22/03/2014, 17:20

... manner based on human intuition Therefore, we believe the hierarchy that we have built is consistent with human intuition, at least around the top level of hyperonymic concepts Conclusion We have ... 2004 Extraction of Hyperonymy of Adjectives from Large Corpora by using the Neural Network Model In Proceedings of the Fourth International Conference on Language Resources and Evaluation, Volume ... second vector or vice versa OVLP of F and T is defined as OVLP( F , T ) = EDR consist of hypernymic concepts represented by sentences On the other hand, our extracted hierarchies consist of hypernyms...

Ngày tải lên: 23/03/2014, 19:20

... market) are stronger and away from the over-the-counter (OTC) market (this too could be done by appropriate use of capital charges or by putting settlement of OTC contracts lower down on the priority ... on the problem list One of the reasons why the crisis hasn’t been more damaging is that most banks, especially large ones, went into the crisis with high bank capital A third feature of the ongoing ... along with a $160 billion fiscal policy package Aim: To cushion the real economy and keep it from feeding back negatively on the financial sector Element Large-scale liquidity injections (by...

Ngày tải lên: 29/03/2014, 03:21

... classification 96.10 NEC adjusts semiconductor production downward I 96.12 NEC postpones semiconductor production plant construction 97.1 97.4 NEC invests ¥ 40 billion for next generation semiconductor ... classification and navigation W ~ W I H Classification and Navigation X~; Conventional keyword -based retrieval does not consider logical relationships between keywords For example, the condition, "NEC ... 5W1H Information Extraction 5W1H extraction was done by a case -based shallow parsing (CBSP) model based on the algorithm used in the VENIEX, Japanese information extraction system (Muraki et al.,...

Ngày tải lên: 31/03/2014, 04:20

... 12 Authors' Contribution 13 Design and conception of the study (AGB, TMR); development of the methods for amplification of the ORF59/60 regions (AGB, TMR); Development of the QPCR assays and quantitative ... chloride concentrations were 4.0 mM for the RV2 assay and 2.0 mM for the OSM assay After activation of the polymerase by incubation for minute at 95°C, amplification was performed on a BioRad ... RRV Amplification of the ORF 59/60 junctional region of novel rhadinoviruses To obtain the ORF 59/60 junctional regions between the RDEL motif of ORF 60 and the PQFV motif of ORF 59 of MneRV2, PcyRV2,...

Ngày tải lên: 18/06/2014, 22:20

... 12 Authors' Contribution 13 Design and conception of the study (AGB, TMR); development of the methods for amplification of the ORF59/60 regions (AGB, TMR); Development of the QPCR assays and quantitative ... chloride concentrations were 4.0 mM for the RV2 assay and 2.0 mM for the OSM assay After activation of the polymerase by incubation for minute at 95°C, amplification was performed on a BioRad ... RRV Amplification of the ORF 59/60 junctional region of novel rhadinoviruses To obtain the ORF 59/60 junctional regions between the RDEL motif of ORF 60 and the PQFV motif of ORF 59 of MneRV2, PcyRV2,...

Ngày tải lên: 20/06/2014, 04:20

... Therefore, LAMP -based detection assays would be suitable for on- the-spot detection in the field or primitive laboratories The aim of this study was to develop a novel method for the detection of SBV in ... turbidity due to the accumulation of magnesium pyrophosphate, a by- product of the reaction Prior to the addition of SYBR Green I, the white turbidity of the reaction mixture by magnesium pyrophosphate ... Therefore, 63°C–65°C was considered the optimal temperature range for RTLAMP reaction for the detection of SBV Figure Determination of the optimal temperature of the LAMP Determination of the optimal temperature...

Ngày tải lên: 21/06/2014, 19:20

... expectation maximization (EM) method In this paper, we introduce the utilization of the GMM of the acoustic transfer function based on the ML estimation approach to deal with a room impulse response ... 42.6 30 10 50 Number of training data (words) 3-position 5-position 7-position 9-position Figure 7: Comparison of the different number of training data function is trained by (16) using the separated ... the localization accuracy of the methods using just one microphone decreases as the number of training positions increases On the other hand, the CSP algorithm based on two microphones has high...

Ngày tải lên: 21/06/2014, 22:20

... environment conditions such as monotony of road (MR), and the number of lanes (NL) The inferring of fatigue based on these features is developed by first collecting feature data through questionnaire ... that work only considered the EEG signal Further in that work, the final aggregation of several channels of information sources into one state has not considered the contribution variation of individual ... principle for solutions to these challenges is to “fuse” multiple kinds of signals of information about persons’ contexts, situations, goals, and preferences [12] Along this line of thinking, a few...

Ngày tải lên: 22/06/2014, 19:20

... beendonein the areaof definingProbabilityof Detection( POD) curvesfor variousforms of Non-Destructive Evaluation(NDE), andmost of this work hasbeenconcentrated the area in of crack detectionin ... [pc(dlla) When + p_(d21a)] the conditional = probability the probability of detection the probability of detection p_(dlla) (3.2-3) under density function the condition for damage p_(dl a) is evaluated ... procedures configurations, procedures configurations, moisture, shortcoming reliability conventional traditional of composite that temperature, for these Consider, design conservative assumes concepts,...

Ngày tải lên: 27/07/2014, 23:46

... assessed by microtiter plate assay Each point is the average of triplicate determinations at each of the target concentrations tested and the error bars represent one standard deviation A detection ... limit of 0.1 nM was obtained based on the value of the lowest concentration tested to be above the value of the negative control plus three times the standard deviation of the negative control ... deviation The detection limit was found to be as low as 10 CFU based on the value of the lowest CFU tested to be above the value of the negative control plus three times the standard deviation of the...

Ngày tải lên: 07/08/2014, 23:22

... a consensus of a number of whole-genome trees based on gene-content comparison among all archaeal genomes does not recover the monophyly of Euryarchaeota, as Halobacteriales are at the base of ... grouping based on the structure of these operons, except for the confirmation of closely related species Coefficient of correlation R = 0.88 Translation 44 29 14 0.00 0.00 14 29 Transcription 44 ... and concatenated into two large fusions, one consisting of 14 proteins of the transcription apparatus (3,275 amino-acid positions), the other consisting of 53 ribosomal proteins (6,377 positions)...

Ngày tải lên: 09/08/2014, 20:21

... risks of transfusion-transmitted viral infections based on rates of detection of recently infected donors Transfusion 2005, 45:254-264 Fabrizi F, Poordad FF, Martin P: Hepatitis C infection and ... increase the probability of detection Third, compared with two commercial HCV detection assays (BIOER and Kehua HCV fluorescence detection kits), the duplex primer/probe assay has many advantages ... RT-PCR assay was lower than that of the two commercial HCV detection assays Fourth, the sensitivity of the duplex primer/probe assay is high and can be compared with that of the CAP/CTM assay...

Ngày tải lên: 12/08/2014, 04:20

... numbers of pVP3 plasmid DNA were 2.76 × 1011 copies/ μL Development and optimization of FQ-PCR and conventional PCR After the optimization of FQ-PCR, we selected the final concentrations of each ... designed on the basis of the highly conserved VP3 region of GPV (GenBank Accession No U25749) Primers and probe were designed by using the Primer Premier software (version 5.0) The position and ... water From each dilution, μL was used as a template and subjected to the conventional PCR and FQ-PCR protocol The detection limit of the conventional PCR was determined based on the Fifty goslings...

Ngày tải lên: 12/08/2014, 04:20