application of porphyrin alkaloid modified gold nanoparticles

... influence of gold nanoparticle thus reveals the nontoxic nature of the gold nanoparticle in the bacterial system (E coli) Hence, it can be used for biological applications with least chances of cytotoxicity ... be modified and channelized for human benefit Proper knowledge of these interactions can lead to a safe era of nanotechnology without threat of human health risk Methods A) Preparation of Nanoparticles ... Glutathione modified Gold (Au) Nanoparticle C) Microscopic Study Typically, 3.0 mM of glutathione was dissolved in 40 mL of distilled water, and 1.0 mM of HAuCl4 was dissolved in 80 mL of methanol...

Ngày tải lên: 11/08/2014, 00:23

... result indicates that the gold nanoparticles can quench the fluorescence of AFP Fluorescence emission spectra of solution containing both AFP and gold colloid with different gold particle content ... rates of phosphors attached to gold nanoparticles Nano Lett 7, 1941–1946 (2007) L Ao, F Gao, B Pan, R He, D Cui, Fluoroimmunoassay for antigen based on fluorescence quenching signal of gold nanoparticles ... Although the fluorescence spectral properties of AFP have already been studied [20], the effect of gold nanoparticles on the fluorescence emission of AFP has seldom been reported Especially, when...

Ngày tải lên: 21/06/2014, 17:20

... with gold NPs in the same solution In agar-well-diffusion experiments, we also made a series of twofold dilutions of the freegentamicin solution and of the gentamicin–NP mixture The formation of ... the free antibiotic and its mixture with NPs were diluted twofold, the diameter of the zone of Fig a Zones of inhibition of the growth of E coli K12 on solid LB medium Wells 1, 3, and received ... Inhibition-zone diameter [mm] Fig a Zones of inhibition of the growth of E coli K12 upon application of gentamicin (1) and gentamicin–NP mixtures at particle concentrations of 0.1 (2), 0.5 (3), and 1.0 mM...

Ngày tải lên: 22/06/2014, 00:20

... M A Why gold nanoparticles are more precious than pretty gold: Noble metal surface plasmon resonance and its enhancement of the radiative and nonradiative properties of nanocrystals of different ... Federal Government, of a GOA grant from K.U Leuven and of long-term structural fundingÀMethusalem funding by the Flemish Government Professor J Billen of the Laboratory for Entomology of K.U Leuven, ... plasmon resonance (LSPR) of gold colloids J Phys Chem B 2004, 108 (37), 13963–13971 (35) Lee, K S.; El-Sayed, M A Gold and silver nanoparticles in sensing and imaging: Sensitivity of plasmon response...

Ngày tải lên: 18/09/2013, 21:27

... signals of the element inside Table lists the binding energy values of Fe, Si, O, N, and Ti resolved from XPS spectra of the above three different NPs In three cases, Page of 15 the value of binding ... Laboratory of Artificial Micro- and Nano-structures of Ministry of Education, Wuhan University, Wuhan 430072, People’s Republic of China Center for Electron Microscopy and School of Physics and ... reacted with the -OH of the APTES molecules leading to the formation of Si-O bonds and leaving the terminal -NH2 groups available for immobilization of TiO2 [41] The immobilization of TiO2 can be explained...

Ngày tải lên: 20/06/2014, 22:20

... stability of gold nanoparticles and their functionalization by cysteine Gold Bull 2009, 42:113-123 Jana NR, Gearheart L, Murphy CJ: Seeding growth for size control of 5-40 nm diameter gold nanoparticles ... Effect of CO flow rate on nanoparticle spectral profile Normalized UV-visible spectra of nanoparticles synthesized from a chloroauric acid concentration of 0.03 mM aerated at flow rates of 16.9, ... dependence of the plasmon absorption of colloidal gold nanoparticles J Phys Chem B 1999, 103:4212-4217 Jain PK, Qian W, El-Sayed MA: Ultrafast cooling of photexcited electrons in gold nanoparticle-thiolated...

Ngày tải lên: 21/06/2014, 03:20

... the conversion of the Au(III), X the value of any of the five parameters of each broth, Cov(X, Y) the covariance value and rxy the correlation coefficient of X and Y with a range of [-1, 1], E the ... illustrate the mechanism underlying biosynthesis of metal NPs To contribute to the determination of biocompounds involved in biosynthesis of gold nanoparticles (GNPs) by foliar broths, a statistical ... Original pH of the broths versus conversion of Au(III) the content of flavonoids, reducing sugars and proteins, as well as the anti-oxidant capability of the foliar broths should reflect the role of each...

Ngày tải lên: 21/06/2014, 17:20

... confirms that the particles are of pure gold TEM analysis of the diameters of 250 nanoparticles yields an average diameter of 5.2 nm The polydispersity of these nanoparticles is 1.1 nm After the ... behavior of the gold nanoparticles, a copper grid covered with gold nanoparticles was placed in an oven and the temperature was raised to and kept constant at 300 °C for 30 After the annealing, the gold ... on the study of coalescence behavior of the gold nanoparticles here Extensive HRTEM observations demonstrate that most gold particles have an elongated shape and there are two types of coalescence...

Ngày tải lên: 22/06/2014, 00:20

... brain, heart, and spleen were isolated, and organ weights of all mice were measured Preparation of Gold Nanoparticles Gold nanoparticles of diameter 3, 5, 8, 12, 17, 37, 50, and 100 nm were synthesized ... mL of 0.25 mM HAuCl4 to 90 mL of H2O and stirred for at 25 °C Two milliliters of 38.8 mM sodium citrate were added to the solution and stirred for min, followed by the addition of 0.6 mL of freshly ... lm, a step size of lm, 300 300 pixels, a scanning velocity of lm/ms, and a sampling rate of 80 kHz Laser power was set at 30 mW for 870 nm and 40 mW for 1064 nm The wavelengths of the pump and...

Ngày tải lên: 22/06/2014, 00:20

... NPs at doses of 0.5, 1.0, 4.0, and 11 nC/cm versus the v/v% ratio of MPTS/ OTS binary mixture The error bars represent the standard errors of the mean of the NNDs Fig SEM images of FIB nanolithography ... was the highest The lateral sizes of Au NPs were constant at diameters of *24 nm At lower doses Fig SEM images of FIB nanolithography aligned Au NPs at a dose of 0.5 nC/cm refilled with (a) 20 ... Consortium (OPC), and CRC Program of the Government of Canada for their kind financial support and the Western Nanofabrication Facility for the availability of FIB and SEM We also thank Todd Simpson...

Ngày tải lên: 22/06/2014, 00:20

... infrared typical PL of thiol stabilized Au nanoparticles [20] A difference of the positions of PL emission peaks of hybrids (1) and (2) is likely due to the different chemical structure of the organometallic ... organometallic complexes open a new access to the preparation of systems that can be easily used for the stabilization of gold nanoclusters Gold nanoparticles were prepared with a modified two-phase procedure, ... TEM micrograph of the same diluted sample with isolated Au nanocrystals of spherical shape and of an average apparent size of about nm The TEM pictures evidence the existence of highly perfect...

Ngày tải lên: 22/06/2014, 01:20

... protocol [27] The size of Gnps obtained by this process was 14 nm Preparation of Conjugated Gold Nanoparticles using Antibiotics as Template The combined reducing property of sodium borohydride ... microscope (JEOL JSM 5200) Experimental Procedures MIC Study of Free and Gnps Conjugated Antibiotics Preparation of Bare Gold Nanoparticles MIC of ampicillin, streptomycin and kanamycin along with their ... etc For successful application of nano-antibiotic conjugation, apart from better delivery, their activities should be evaluated properly because the amount of antibiotics often given for therapy...

Ngày tải lên: 22/06/2014, 06:20

... protocol [27] The size of Gnps obtained by this process was 14 nm Preparation of Conjugated Gold Nanoparticles using Antibiotics as Template The combined reducing property of sodium borohydride ... microscope (JEOL JSM 5200) Experimental Procedures MIC Study of Free and Gnps Conjugated Antibiotics Preparation of Bare Gold Nanoparticles MIC of ampicillin, streptomycin and kanamycin along with their ... etc For successful application of nano-antibiotic conjugation, apart from better delivery, their activities should be evaluated properly because the amount of antibiotics often given for therapy...

Ngày tải lên: 22/06/2014, 19:20

... film embedded with Ag nanoparticles is golden yellow in color and Au embedded composite film has pink color The rate of formation of Ag nanoparticles is higher than that of Au nanoparticles The surface ... consisting of metallic Ag without any AgO TEM images of Au nanoparticles of 3.3 and 5.4 mM HAuCl4 solutions for a dipping time of 30 are shown in Fig The particle size as well as the amount of Au ... the formation of Ag nanoparticles is very facile because the diffusion of Ag+ is higher than that of Au3+ and also due to hydrophilic nature of Ag [36] Spontaneous self-assembly of silicotungstate...

Ngày tải lên: 22/06/2014, 22:20

... rice genome The results of PCRs would confirm the appearance of the AtAOS2 gene in the transgenic rice genome MATERIALS AND METHODS 2.1 Extraction of rice DNA The leaves of one-month-old wild-type ... expression of soybean allene oxide synthases, Biosci Biotechnol Biochem 71(2), 491-498 Schaller, F (2001) Enzymes of the biosynthesis of octadecanoid-derived signaling molecules, Journal of experimental ... (Fig 5) Nguyen Thi Thuy Hanh, Han Oksoo 15 Application of PCR to identify the transgene (Arabidopsis thaliana allene oxide synthase2) Fig Alignment of rice AOS5 (AK066137) with the primer pair...

Ngày tải lên: 06/08/2014, 18:21

... rof sisylana rehtruf rof nesohc saw ASILE-P rof 001.0 fo ffotuc eht ,eroferehT tniop ffotuc no gnidneped yrav ot dnuof neeb evah sASILE fo seulav pS dna eS eht ,revoeroM BTP rof yllaicepse ,slamina ... ylsuoiverp sASILE rof detroper neeb sah hcihw ,tnemeerga fo level wol a dewohs sASILE owt eht rof eulav appak eht ,ASILE-P rof 001.0 fo ffotuc a gnisU stluseR ni evoba denoitnem snosaer eht rof sisylana ... yranireteV rof margorP 12 aeroK niarB eht yb trap ni dednuf saw yduts sihT stnemgdelwonkcA noitcefni BTP yfirev ot sdohtem rehto yb dewollof slamina laudividni rof ro dreh detcefni rof tset gnineercs...

Ngày tải lên: 07/08/2014, 18:21

... generator of heating of solutions of gold nanoparticles (GNPs) exposed to external radiofrequency (RF) fields at Thermographic results power outputs Thermographic results of heating of solutions of gold ... Gold Nanoparticles Figure µM/L gold nanoparticles with 67 Transmission electron microscopy of Panc-1 cells treated Transmission electron microscopy of Panc-1 cells treated with 67 µM/L gold nanoparticles ... water with increasing concentrations of GNPs treated at 200 W of power B RF treatment at 400 W of power C RF treatment at 600 W of power D RF treatment at 800 W of power Heating curves which conclude...

Ngày tải lên: 11/08/2014, 00:22

... to determine the diameter of nanoparticles The range of observed diameter of the synthesized gold nanoparticles was about 50 nm B Representative XRD pattern of gold nanoparticles synthesized ... pancreas of diabetic mice Histological sections of pancreas of experimental group of mice after treatment with gold nanoparticles for 45 days revealing the preventive effect of gold nanoparticles ... mice with gold nanoparticles (p < 0.05) Figure Influence of gold nanoparticles over the anti-oxidant system in experimental mice The gold nanoparticles restored the elevated level of antioxidant...

Ngày tải lên: 11/08/2014, 00:22

... cellular uptake of DNA -modified gold nanoparticles Nano Lett 2007, 7:3818-3821 24 Rosi NL, Giljohann DA, Thaxton CS, Lytton-Jean AK, Han MS, Mirkin CA: Oligonucleotide -modified gold nanoparticles ... http://www.jnanobiotechnology.com/content/8/1/21 Page of 11 Figure Verification of the activity of aptamer-conjugated AuNPs (A) The shift of the SPR maximum clearly indicates the formation of agglomerates in the presence of streptavidin ... detection of streptavidin, using aptamer-conjugated gold nanoparticles (A) ESEM image of nanoparticles bound to streptavidin immobilized on the nitrocellulose membrane (B) negative net charge of the...

Ngày tải lên: 11/08/2014, 00:22

... the role of surfactants in the formation of directed morphologies is a matter of thorough investigations of nucleation, growth and also of interaction energies of surfactants with that of the embryos ... detection/isolation of bacteria, also depends on the size of magnetic nanoparticles The size of Fe O nanoparticles should be such that it can allow the presence of sufficient number of ligands to achieve ... 30 mg of GA modified Fe3O4 (b) Cartoon representation of the interaction of bacteria with that of fish collagen List of abbreviations GA: Gum Arabic; CCGAMION: Collagen coated gum Arabic modified...

Ngày tải lên: 11/08/2014, 00:22