application of fusogenic virus liposomes to gene therapy of intractable diseases

... Adenovirus Life Cycle Entry of Adenovirus into Cells 31 Prem Seth Binding of Adenovirus to the Cell Receptor, and its Entry into the Endosomes 31 Adenovirus-Mediated Lysis of ... Adenovirus Life Cycle Entry of Adenovirus into Cells 31 Prem Seth Binding of Adenovirus to the Cell Receptor, and its Entry into the Endosomes 31 Adenovirus-Mediated Lysis of ... Role of Low pH and Penton Base 33 Vectorial Movement of the Adenovirus into the Nucleus 33 Conclusion 35 Early Gene Expression 39 Philip E Branton Adenovirus Genes...

Ngày tải lên: 10/04/2014, 10:58

... switching of human hemoglobin Gene therapy for β-thalassemia As a classic gene model for human genetics,β-globin gene has been extensively studied in the fields of gene structure, gene evolution, gene ... people to try to develop new viral vectors for βthalassemia gene therapy, such as lentivirus (LV), adeno associated virus (AAV),et al The well-known lentivirus is human immunodeficiency virus ... Kay MA: From virus evolution to vector revolution: use of naturally occurring serotypes of adeno-associated virus (AAV) as novel vectors for human gene therapy Curr Gene Ther 2003; 3: 281-304 [21]...

Ngày tải lên: 05/09/2015, 11:13

... possible to establish tolerance to the transgene product human factor IX by these early injections and to obtain long-term therapeutic levels in immunocompetent mice Here, the transgene products of ... vector after the second injection of viral vector Thus, the early exposure of the immune system to viral vector capsid allows a successful second application but does not induce an immunotolerance ... the existence of a "window of opportunity" to induce tolerance against the viral capsid in repeated exposures Page of (page number not for citation purposes) Genetic Vaccines and Therapy 2009,...

Ngày tải lên: 14/08/2014, 19:22

... suppression of HCV replication Baculoviruses appear to be useful viral vectors, not only for the abundant expression of foreign genes in insect cells, but also for efficient gene delivery to the hepatoma ... increased to be applicable for human gene therapy [19] The Epstein Barr virus (EBV) plasmid is a replicating episomal vector that has been developed to overcome the problem of rapid elimination of intracellularly-delivered ... development of effective anti-HCV drugs Gene silencing by RNA interference is a promising approach to elucidate gene function and to inhibit certain RNA viruses such as HCV [32-34] Delivery of siRNA to...

Ngày tải lên: 12/08/2014, 04:20

... one of the most frequently employed forms of gene delivery in somatic gene therapy [15, 16, 17] Additionally, RVVs are able to deliver genes to the germline, as established in animal transgenesis ... into hidden factors has been the notion of the existence of ‘inhibitory’ factors (IFs) associated with sperm cells These IFs are envisaged to prevent exogenous DNA uptake so as to protect the genetic ... if SMGT were to prove effective as a means to gene transfer, it would be fundamentally limited in the context of human germline gene therapy due to its unsuitability as a means of gene targeting...

Ngày tải lên: 03/11/2012, 10:01

... Samulski J, Leone P: Clinical protocol Gene therapy of Canavan disease: AAV-2 vector for neurosurgical delivery of aspartoacylase gene (ASPA) to the human brain Hum Gene Ther 2002, 13:1391-1412 McPhee ... to the forefront of gene therapy in the past decade The exponential progress of AAV-based vectors has been made possible by the advances in the knowledge of the virology and biology of this virus, ... two major hurdles to gene therapy, safety and efficacy, remain roadblocks to the widespread application of gene therapy as a standard medical treatment for disease Improvement of efficacy can be...

Ngày tải lên: 18/06/2014, 18:20

... Samulski J, Leone P: Clinical protocol Gene therapy of Canavan disease: AAV-2 vector for neurosurgical delivery of aspartoacylase gene (ASPA) to the human brain Hum Gene Ther 2002, 13:1391-1412 McPhee ... to the forefront of gene therapy in the past decade The exponential progress of AAV-based vectors has been made possible by the advances in the knowledge of the virology and biology of this virus, ... two major hurdles to gene therapy, safety and efficacy, remain roadblocks to the widespread application of gene therapy as a standard medical treatment for disease Improvement of efficacy can be...

Ngày tải lên: 20/06/2014, 01:20

... quantification of virus load as a measure of replication of HBV in patients with chronic liver disease Prior to the development of the polymerase chain reaction (PCR) a number of hybridisation ... Quantification of HBV DNA may be a more useful measure than HBeAg as genetic variants of HBV may continue to replicate at high level without secreting HBeAg Quantification of HBV DNA can be useful to assess ... Monitoring of HBV DNA loads in subjects receiving lamivudine therapy The level of HBV DNA was measured in 16 asymptomatic carriers (eight HBeAg positive and eight HBeAg negative) on daily dose of...

Ngày tải lên: 20/06/2014, 01:20

... continuity of effort to bring gene therapy into rheumatologic clinical practice Summary and future directions During the decade under review, the application of gene therapy in rheumatic diseases ... [31] The occurrence of leukemia in humans as a result of insertional mutagenesis using retrovirus vectors, coupled to the high cost of ex vivo gene therapy using passaged autologous cells, has ... little risk of vector escaping to nontarget organs Many of the principles described above in the context of RA also apply to Sjögren syndrome [88] Although adenovirus vectors transfer genes to the...

Ngày tải lên: 09/08/2014, 01:22

... according to response to therapy Effects of adalimumab therapy on synovial gene expression profiles Next, we investigated the effects of adalimumab therapy on global gene expression profiles of synovial ... Affymetrix GeneChip Operating Software (GCOS) The frequency of positive genes (genes with a flag present) was between 45% and 55% on each slide After scaling of all probe sets to a value of 100, ... mean and standard error of the mean of gene expression, relative to the mean gene expression in a standard sample normalized to *P < 0.05 CCL5, chemokine ligand 5; CTLA4, cytotoxic T-lymphocyte-associated...

Ngày tải lên: 09/08/2014, 14:20

Ngày tải lên: 12/08/2014, 04:21

... targets The poor response of the laboratory simian lentiviruses to NNRTIs prompted some to replace the reverse transcriptase (RT) gene of the simian lentivirus with a gene encoding HIV-1 RT [18] ... apes in the laboratory The simian AIDS model presents its own profile of response to HIV-1 drugs, rendering it difficult to treat with the ART protocols adopted for treatment of HIV1/AIDS For ... restriction factor to infection by primate lentiviruses [14] Upon clarification of these issues, this simian/human immunodeficiency virus (SHIV) chimera could become an extremely useful tool to model...

Ngày tải lên: 12/08/2014, 23:23

... multiple shRNA gene therapies may need to be combined with a Class I inhibitor to allow Tx cells to survive and mitigate the effects of HIV Methods This stochastic model of gene therapy for HIV ... pre-run for 100 days to mimic the natural course of infection prior to gene therapy This enabled HIV to accumulate random mutations and develop into a pool of variant strains to simulate natural ... In summary, resistance to gene therapy appears to differ from that for antiretroviral therapy Although HSC gene therapy aims to establish a large protected population of target CD4+ T cells,...

Ngày tải lên: 13/08/2014, 01:20

... fmol) of plasmid DNA harboring either HSVTK, Luc, or HSV-TK-Luc transgenes, respectively As all vectors were of equal size, equal amounts of DNA corresponded to equimolar amounts of plasmid Cytotoxic ... quantification of the cytotoxic effect of HSV-TK by BLI Correlation of enzymatic activities Fusion of two enzymes is the only way to guarantee stoichiometric, and thus correlated expression of both ... Positron-emission tomography of vector-mediated gene expression in gene therapy for gliomas Lancet 2001, 358:727-729 Page 12 of 13 (page number not for citation purposes) Genetic Vaccines and Therapy 2004,...

Ngày tải lên: 14/08/2014, 19:22

... efforts need to be made to assess and reduce the rate of transfer of retroviral genes Oncogene capture The mechanism of oncogene capture appears to be dependent on the generation of a chimeric ... chromosomal genes In contrast to oncogene activation, the oncogenic potential of some retroviruses maps to the env gene sequences For example, the SU protein (p55) of the polycythemic strain of Friend virus ... exposure to less cytokines needs to be fully explored 6) In general the limitations of vectors should be taken into account when designing gene therapy protocols For example, in the case of X-SCID, it...

Ngày tải lên: 14/08/2014, 19:22

... delivery of adenoviruses Baculoviruses (Autographa californica multiple nucleopolyhedrovirus) are emerging as vectors for gene therapy Compared with conventional viral vectors, the baculovirus has ... 34 3.1 Construction of Baculoviral Vectors…………………………35 3.2 In Vitro Sensitivity of Glioma Cells to Activated Prodrug…………36 3.3 Cytotoxic Effects of Prodrugs without Suicide Gene ……… …38 3.4 In ... Suicide Gene/ Prodrug System Used in Gene Therapy Gene therapy is one of the most promising new frontiers in medical therapeutic intervention, especially in tumor therapy Currently used applications...

Ngày tải lên: 12/10/2015, 17:34

... findings of this experiment, accurate gene sequencing is also a critical factor in the determination of the fate of gene expression Any mismatched base pair may lead to the inability of a gene to express ... although the deletion of the E1 and E2b genes and the use of homologous (rat) BMP genes not improve the osteogenic potential of direct BMP adenovirus vector gene therapy, the results of this study indicate ... needed to produce the helper-dependent vector and the possible roles of viral gene products in the process of bone formation need to be clarified further An adenoviral vector with deletions of E1,...

Ngày tải lên: 31/10/2012, 17:08

... and the aims of the study, it is necessary to limit the study to the areas which are manageable Benefits of the application of games in teaching grammar create the effectiveness of students’ ... Organization: The best way to gauge this is to pay attention to students for the first few days to see what length of time works the best for them Additionally, try to have everything ready to go before the ... wants to make sure activities appeal to all sorts learning styles, so even when using games to teach grammar he/she will want to vary the types of things he/she expects students to - Respect: To...

Ngày tải lên: 07/11/2012, 14:44

... extended to two risk factors, while the portfolio consists of vanilla options on stocks 3.1 One Risk Factor To facilitate a better understanding of the behaviour of generalized Pareto distribution ... stock and short stock In these cases, we have only one risk factor the price log returns To facilitate a better understanding of the behaviour of generalized Pareto distribution when applied to ... Equity stocks The risk factor under consideration is the one-day log-returns of the stock prices 3.1.1 UBS stocks We start with the analysis of UBS stock returns, a case of a highly liquid stock...

Ngày tải lên: 27/04/2013, 11:17