apoptosis and its role in inflammatory disease

... wild-type occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon played a major role in the induction of mitochondria-mediated apoptosis and the ... occludin in apoptosis and invasion A C B D E F Fig Identification of a human occludin splice variant in liver cell lines (A) Occludin protein expression was examined with an antibody to the N-terminus ... which inhibits IP3R and thus calcium release from the ER, we suggest that an increase in the intracellular calcium level is a possible explanation for occludin-induced apoptosis and reduced invasiveness...

Ngày tải lên: 18/02/2014, 18:20

... proteins, Vpr, has been shown to dysregulate several host cellular functions including apoptosis in infected and uninfected bystander target cells through its interaction with host cellular proteins ... found in Drosophila Drosophila MASK (dMASK) has been implicated in cell survival and may play a role in promoting proliferation and preventing apoptosis [8] dMASK mediates receptor tyrosine kinase ... measured and represented in relative light units (RLU) Figure represents one of three independent experiments understand the pathway(s) and mechanism(s) involved in VBARP and its regulation apoptosis...

Ngày tải lên: 07/03/2014, 21:20

... very-KIND-MAP2 interaction v-KIND KIND2 KIND2-1 KIND2-2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... the structural and functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across ... deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 domain (B) KIND2 domain anchors v-KIND to dendrites Flag-tagged v-KIND, DKIND1, DKIND2, DKIND1 + 2, DRasN, DGEF, KIND1 or KIND2 together with...

Ngày tải lên: 14/02/2014, 19:20

... copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain swapping and forms ... C-terminus (box in Fig 1) at sites: 92, 75, 66 and 58 As histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although ... binding on the secondary structure was determined using CD in the far UV To prepare proteins without Cu2+, part of each protein 4252 Fig Comparison of copper binding by stefin A and stefin B using...

Ngày tải lên: 19/02/2014, 06:20

... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important role in leucine catabolism AUH was overproduced in E coli and characterized by measuring ... (c.719C>T) in exon and an insertion mutation c.613–614insA This insertion causes a frameshift that starts at Met205 and leads to the introduction of a stop codon after four amino acids The intra-assay ... Characterisation and mitochondrial localisation of AUH, an AU-specific RNA-binding enoyl-CoA hydratase Gene 228, 85–91 Nakagawa J & Moroni C (1997) A 20-amino-acid autonomous RNA-binding domain contained in...

Ngày tải lên: 19/02/2014, 07:20

... case in point, formation of huntingtin-containing inclusion bodies in Huntington’s disease encourages cell survival, whereas monomers and small inclusion bodies of huntingtin, a protein possessing ... opaque with age and contain many inclusion bodies reactive with antibody to aB-crystallin, but not to b- and c-crystallin, suggesting an important role for aA-crystallin in maintaining lens transparency ... aB-Crystallin R120 corresponds to aA-crystallin R116 and both are conserved a-crystallin domain arginines R120G aB-crystallin permits intermediate filament self association in vitro, although binding...

Ngày tải lên: 19/02/2014, 18:20

... (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations ... were removed and minced with a surgical knife The minced tissue was wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation ... were incubated at 37 °C in a humidified 5% CO2 incubator in the absence (control) and presence of increasing concentrations of inhibitors for 10 days, and the number of molting larvae was determined...

Ngày tải lên: 20/02/2014, 11:20

... Printing Office, Washington, D.C., 2004 SOLAR AND SPACE PHYSICS AND ITS ROLE IN SPACE EXPLORATION the fundamental role of solar and space physics research both in scientific exploration and in ... principal operating agency of both the National Academy of Sciences and the National Academy of Engineering in providing services to the government, the public, and the scientific and engineering ... Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding engineers It is autonomous in its administration and in the...

Ngày tải lên: 14/03/2014, 10:20

... no membrane-spanning domains in PEX14 have been unambiguously identified and it was observed in mammalian cells and S cerevisiae that both the N-terminal and the C-terminal domain are at the cytosolic ... synthesized proteins in the cytosol due to the inability to import matrix proteins by the fraction of growing and dividing glycosomes The miscompartmentation of PTS-1 and PTS-2 proteins (GAPDH and ALD, ... approximately 0.5 to induce the expression of the protein and growth was continued for h Cells were collected by centrifugation (3000 g, 10 min, °C) and resuspended in cell lysis buffer containing 50 mM...

Ngày tải lên: 23/03/2014, 17:21

... development of inflammatory arthritis [14-16] This highlights the critical role of IL-1ra in the constitutive maintenance of peripheral tolerance, and in counterbalancing the proinflammatory effects ... Available online http://arthritis-research.com/content/6/1/26 known roles in tissue inflammation, and damage in innate immunity Besides its capacity to drive the production of IFN-γ and IL-2 by ... tolerance in the thymus Competing interests None declared References 5.* 10 11 12 13.* 14 15 16 Delon J, Stoll S, Germain RN: Imaging of T-cell interactions with antigen presenting cells in culture and...

Ngày tải lên: 09/08/2014, 01:23

... potential benefits of statin therapy on inflammatory airway disease After priming and intra-nasal ovalbumin challenge, reductions in inflammatory cell infiltrate and eosinophilia in bronchoalveolar ... by CRP remains in debate [14] Statin-induced reductions in CRP have been shown both in patients with and without established cardiovascular disease [15] Interestingly, these anti -inflammatory ... Kwak and colleagues first showed that statins inhibit interferon-γ (IFN-γ)-inducible MHC-II expression in various cell types including endothelial cells and macrophages, thereby inhibiting MHC-II-mediated...

Ngày tải lên: 09/08/2014, 06:22

... cancer cell lines, as does cell proliferation Thus, Per2 loss during intestinal tumorigenesis may, in part, act through upregulation of b-catenin, increasing intestinal b-catenin signaling and cell ... cell proliferation Also, increase in small-intestinal mucosa b-catenin in Per2m/m mice is associated with an increase in MYC protein, again a circadian regulated and b-catenin target gene [89] Furthermore, ... regulating the circadian clock include acetylation, phosphorylation, ubiquitination and sumoylation In terms of phosphorylation, Casein kinase epsilon (CK1ε) and Casein kinase delta (CK1ε and CK1δ),...

Ngày tải lên: 10/08/2014, 09:20

... HIF-1 Hexokinase-1 HIF-1 Hexokinase-2 HIF-1 Insulin-like growth factor-2 (IGF-2) HIF-1 IGF-binding protein-1 HIF-1 IGF-binding protein-2 HIF-1 IGF-binding protein-3 HIF-1 Interleukin-6 NF-IL6 ... cytokine with anti -inflammatory properties, it might counteract the effects of proinflammatory cytokines For example, the expression of IL-1 and IL-8 are also induced in hypoxic ECs [4,5] and ... subunits in pulmonary artery smooth muscle cells J Clin Invest 1997, 100:2347–2353 Author’s affiliation: Institute of Genetic Medicine and Departments of Pediatrics and Medicine, The Johns Hopkins...

Ngày tải lên: 12/08/2014, 18:20

... also increasingly involved in the development and delivery of a range of education and training programmes designed to help individuals working in HIV advance professionally, from administering ... Providing education and training opportunities for its membership is important for any professional society, and the IAS is looking at ways to strategically expand its role in this area in order ... cross-disciplinary linkages and dialogue, and to strengthen the focus on youth – began to be implemented in the planning for the XVI International AIDS Conference (AIDS 2006) in Toronto and will...

Ngày tải lên: 13/08/2014, 09:20

... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... is known, also indicates that PDI is a regulator of inflammatory cytokines Previous studies have demonstrated that proinflammatory cytokines play a critical role in the initiation and progression ... adrenomedullin and adrenomedullin-binding protein-1 in sepsis: down-regulation of proinflammatory cytokines Crit Care Med 2002, 30:2729-2735 Darby NJ, Creighton TE: Functional properties of the individual...

Ngày tải lên: 13/08/2014, 11:22

... transduced into OV90 cells 103 xiv Figure 33 Wild-type TG2 and N-terminal fibronectin binding domain of TG2 induce EMT in OV90 cells Figure 34 104 Wild-type TG2 and N-terminal fibronectin binding domain ... at its protease sensitive hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase ... Tyr583 are involved both in GDP and ATP binding to TG2 The residue Arg580, which is important for GTP/GDP binding, is not involved in ATP binding (Han et al 2010) By purifying the insulin-like growth...

Ngày tải lên: 24/08/2014, 10:58

... expressed predominantly in erythroid cells HRI is regulated by heme through the two heme-binding regions in HRI: an N-terminal domain of HRI and in an insert region in the protein kinase domain of HRI ... cytoplasmic splicing of mRNA encoding the Xbox binding protein1 (XBP1) in mammals and homologue of the mammalian activating transcription factor/cAMP-response element (CRE)-binding protein (HAC1) in yeast ... globin aggregation and 10 Figure eIF2 Kinases, GCN2, HRI, PKR, and PERK regulate translation in response to different stresses Each eIF2 kinase has a conserved protein kinase domain and distinct...

Ngày tải lên: 24/08/2014, 12:32

Ngày tải lên: 14/11/2014, 06:30

... knockdown MIN6 cell line Figure4.2 Increased betagranin storage in BIG3-deficient MIN6 cells Figure4.3 Increased insulin storage in BIG3-deficient MIN6 cells ix Figure4.4 Increased betagranin secretion ... protein recruitment distiguishes the cargo-loaded subdomains from the rest of TGN Curvature-inducing proteins, such as the Bin/amphiphysin/Rvs (BAR) domain family and the GRIP-domain-containing ... endosomes and partial PM, and has a role in both E-cadherin recycling and actin remodeling through activating Arf6 (Hiroi et al., 2006; Someya et al., 2001) FBX8 is named after its N-terminal F-box...

Ngày tải lên: 11/09/2015, 09:15

... genes coding for a range of pro -inflammatory mediators, including interleukin-6, interleukin-8, and tumor necrosis factor α (Schreck et al., 1991) The increased expression of these pro -inflammatory ... factors and H pylori - induced IL-8 production 30 2.6.3 H pylori infection and cell apoptosis 31 2.6.3.1 The intrinsic and extrinsic apoptotic pathways 32 2.6.3.2 Apoptosis in gastric epithelium induced ... Determining the subcellular localization of GGT in H pylori Examining the pathway of GGT in mediating the enhanced gastric epithelial cell apoptosis Identifying the possible role of GGT in the...

Ngày tải lên: 12/09/2015, 09:55